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Citations for Electron Microscopy Sciences :
51 - 100 of 1371 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... the cells were fixed with 1 ml of the fixative (2% paraformaldehyde (Electron Microscopy Sciences) dissolved in 250 mM HEPES [pH 7.4] ...
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bioRxiv - Microbiology 2021Quote: ... Cells were fixed at 18 hpi for 2 h on ice in 2.5% glutaraldehyde (EMS) in 0.1 M cacodylate buffer (CB ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were washed 2 times with 0.1 M Sodium cacodylate (Electron Microscopy Sciences, Hatfield, PA), and post-fixed on ice in 1% v/v reduced Osmium tetroxide ...
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bioRxiv - Immunology 2022Quote: ... The cells were washed and fixed in with 2% PFA (15710-S, Electron Microscopy Sciences) diluted in 0.2% FACS buffer for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... cells were fixed in a final concentration of 2% paraformaldehyde (Cat# 15714S; Electron Microscopy Sciences) and % GFP positive cells were enumerated by flow cytometry (Intellicyt iQue Screener PLUS ...
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bioRxiv - Biochemistry 2020Quote: ... cells at room temperature were fixed for 10 minutes with 2% formaldehyde (Electron Microscopy Sciences 15710), and permeabilized for one hour with 0.1% Triton X-100 (Biorad 1610407 ...
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bioRxiv - Microbiology 2022Quote: ... cells were washed with 1XPBS and fixed for 15 min with 2% paraformaldehyde (Electron Microscopy Sciences) in 1XPBS ...
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bioRxiv - Immunology 2020Quote: ... Cells were fixed using 300-400 µl of 2% paraformaldehyde (Electron Microscopy Sciences, catalog 15712-S), then CD107a surface expression was measured using a modified LSR Fortessa (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixated in a 96U plate in 250 µl of 2% paraformaldehyde (PFA, EMS #15710) in PBS at room temperature for 20 min ...
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bioRxiv - Genetics 2020Quote: ... cells were relaxed using 50mM KCl and then fixed in Karnovsky’s fixative: 2% Glutaraldehyde (EMS, #16000) and 4% paraformaldehyde (EMS ...
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bioRxiv - Genomics 2020Quote: ... and the cells were stained with reduced osmium (2% osmium tetroxide and 1.5% potassium ferrocyanide, EMS) for 30 minutes on ice to further enhance contrast ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed in 4% paraformaldehyde and 2% sucrose in PBS for 10 mins (EMS Diasum). Fixed cells were permeabilized/blocked in 0.2% (v/v ...
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bioRxiv - Bioengineering 2022Quote: ... the coverslips with cells were incubated in 2% osmium tetroxide (Electron Microscopy Sciences, Hatfield PA, US) for 2 h ...
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bioRxiv - Neuroscience 2023Quote: Cells were grown on aclar and then fixed in Karnovsky’s fixative: 2% Glutaraldehyde (EMS Cat# 16000) and 4% para-formaldehyde (EMS Cat# 15700 ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated 2 min on 2% paraformaldehyde (Electron Microscopy Sciences), then incubated for 30 sec on 2% uranyl acetate (aqueous ...
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bioRxiv - Immunology 2019Quote: ... prior to deposition onto 2/2 Quantifoil grids (EMS) that were glow discharged for 10 s directly preceding the deposition in a Vitrobot (Thermo Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... CF-2/2-4C (CF-224C-100) (Electron Microscopy Sciences) cryoEM grids ...
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bioRxiv - Genomics 2021Quote: ... and the cells were fixed with 2 ml of 4% PFA in PBS (Electron Microscopy Sciences, 15710) for 20 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... NIH/3T3 and IMR-90 cells were fixed with 2% paraformaldehyde (PFA) (Electron Microscopy Sciences, cat# 15710) for 8-10 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then fixed for 10 min at room temperature with 2% PFA (Electron Microscopy Sciences) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... 2% paraformaldehyde (EMS) in 0.1 M sodium cacodylate buffer (EMS) ...
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bioRxiv - Neuroscience 2021Quote: ... 2% glutaraldehyde (EMS), 2.2mM CaCl2 diluted in 50 mM MOPS buffer (pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: Quanifoil R 2/2 200 mesh copper grids (EMS #Q2100-CR2) were glow discharged at 25mA for 25s using a Pelco easiGlow system (Ted Pella) ...
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bioRxiv - Neuroscience 2021Quote: ... A small piece (∼2 × 2 mm) containing V1 was cut out and prepared for EM by staining sequentially with 2% osmium tetroxide (EMS) in Sodium Cacodylate buffer ...
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bioRxiv - Biochemistry 2023Quote: ... the same proteins were applied to holey grids with a 2 nm carbon film (Quantifoil Cu 2/2 200 mesh from EMS). Samples were frozen after a total of 145 min on the grids without carbon film and 150 min on the grids with carbon film ...
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bioRxiv - Bioengineering 2022Quote: Degassed 2/2-3C C-flat grids (Electron Microscopy Sciences, Hatfield, PA) were glow discharged for 45 s at 30 mA ...
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bioRxiv - Biophysics 2024Quote: ... Quantifoil R 2/2 copper 200 mesh grids (Electron Microscopy Sciences #Q2100CR2) were glow-discharged for 45 seconds at 15 mA using a PELCO easiGLOW Glow Discharge System to apply a negative charge to their surface ...
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bioRxiv - Neuroscience 2021Quote: ... 2% glutaraldehyde (EMS, USA), 0.2% picric acid (EMS ...
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bioRxiv - Neuroscience 2021Quote: ... 2% glutaraldehyde (EMS 16000), p.H 7.4 ...
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bioRxiv - Developmental Biology 2023Quote: ... and 2% glutaraldehyde (EMS) in 0.1 M cacodylate buffer containing 5 mM CaCl2 (pH 7.4) ...
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bioRxiv - Cell Biology 2023Quote: ... 2% osmium tetroxide (EMS) at at −90℃ for 11hours ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed twice with CyFACS and fixed overnight at 4°C with 2% PFA (Electron Microscopy Sciences) in metal contaminant-free PBS (Rockland) ...
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bioRxiv - Molecular Biology 2020Quote: ... infected and uninfected cells were fixed by rapidly transferring Aclar slabs into Karnovsky’s fixative (2% Glutaraldehyde (EMS cat.#16000) and 4% PFA (EMS cat.#15700 ...
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bioRxiv - Immunology 2020Quote: ... The cells were washed and then fixed in FACS buffer supplemented with 2% PFA (15710-S, Electron Microscopy Sciences) for 15 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were washed with cold flow cytometry buffer and fixed with 2% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA). The samples were stored at 4°C wrapped in foil up to 1 week prior to flow cytometric analysis ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed 3X with CyFACS buffer and fixed overnight at 4°C with 2% PFA (Electron Microscopy Sciences) in metal contaminant-free PBS ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... cells were rinsed with PBS containing calcium and magnesium and fixed using 2% paraformaldehyde (PFA) (Electron Microscopy Sciences # 15710) in PBS for 20 min ...
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bioRxiv - Cancer Biology 2023Quote: ... The next day cells were detached using detachment buffer (0.05% EDTA in PBS) for 5 minutes and harvested in 10% HI-FBS DMEM and fixed with 4% paraformaldehyde (PFA, no-15710, Electron microscopy sciences, USA) in PBS for 10 min at room temperature ...
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bioRxiv - Biophysics 2022Quote: C-Flat 2/2-3C or 1.2/1.3-3C grids (Electron Microscopy Sciences) were glow discharged at 10 mA for 90 seconds ...
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bioRxiv - Cell Biology 2022Quote: ... R 2/2 carbon film gold support Quantifoil grids (Q2100AR2; Electron Microscopy Sciences) were loaded into the vacuum chamber and carbon was deposited under high vacuum with stage rotation ...
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bioRxiv - Microbiology 2021Quote: ... Three μL of cell culture were applied to Cflat-2/1 200 mesh copper or gold grids (Electron Microscopy Sciences) glow discharged for 30 seconds at 15 mA ...
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bioRxiv - Cell Biology 2022Quote: Isolated mouse islets and MIN6 cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde in 0.1M Sodium Cacodylate buffer (cat. no. 15960-01 Electron Microscopy Sciences) for 2 hours at 4°C and then stained in 1.0% osmium tetroxide (cat ...
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bioRxiv - Plant Biology 2023Quote: ... and the resulting cell pellets were fixed using a mixture of 2% [v/v] paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) and 2% [v/v] glutaraldehyde (Agar Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were fixed in 0.1 M cacodylate buffer (pH 7.4) with 2% glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) and 2% paraformaldehyde (Electron Microscopy Sciences ...
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bioRxiv - Neuroscience 2024Quote: ... Neurons were then fixed with 2% glutaraldehyde and 2% paraformaldehyde (Electron Microscopy Sciences, #15710) in 0.1 M sodium cacodylate buffer ...
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bioRxiv - Developmental Biology 2023Quote: Lungs were fixed in EM fixation buffer (2% glutaraldehyde, 2% paraformaldehyde (Electron Microscopy Sciences), 0.1% calcium chloride in 0.1M sodium cacodylate buffer pH 7.2 ...
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bioRxiv - Neuroscience 2021Quote: ... 2% paraformaldehyde (Electron Microscopy Sciences) in cacodylate buffer (0.15 M sodium cacodylate [Electron Microscopy Sciences] and 0.04%CaCl2 [MilliporeSigma]) ...
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bioRxiv - Synthetic Biology 2019Quote: ... 2% glutaraldehyde (Electron Microscopy Sciences) and incubated on a roller for 20 mins at room temperature (RT) ...
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bioRxiv - Neuroscience 2020Quote: ... and 2% paraformaldehyde (P16148 EMS) in sodium cacodylate buffer 0.15 M (pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... 2 % paraformaldehyde (Electron Microscopy Sciences), 1 mM CaCl2 in PBS ...