Labshake search
Citations for Electron Microscopy Sciences :
51 - 100 of 129 citations for Mouse Protein DEK DEK ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2022Quote: ... Mouse embryonic stem cells were plated on Human recombinant laminin 511-coated coverslips (Electron Microscopy Sciences, Cat. 72196-25). Cells were then fixed using 4% formaldehyde (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... including Goat-anti-Human IgG (H&L) and Goat-anti-Mouse IgG (H&L) (Electron Microscopy Sciences, Hatfield, PA) (1:20 dilution in 1% BSA ...
-
bioRxiv - Cell Biology 2020Quote: ... infiltrated with Epon resin (Embed-812 kit, EMS 14121), and flat-embedded in the same resin between two pieces of ACLAR embedding film with a 50 μm thickness (EMS 50426 ...
-
bioRxiv - Biophysics 2021Quote: A 10 μl volume of 5 times diluted aggregated sample (initial αS concentration = 50 µM and N-protein concentration = 0.2 – 1 μM) was deposited onto freshly cleaved mica (Muscovite mica, V-1quality, EMS, US) and left to rest for 5 min ...
-
bioRxiv - Biophysics 2021Quote: The nanodisc incorporated protein was individually applied to freshly glow-discharged Quantifoil R1.2/1.3 Cu300 mesh grids (Electron Microscopy Sciences). Grids were blotted for 4 s under 100% humidity and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 3.5 μl of protein solution were applied onto the glow discharged Quantifoil Cu R1.2/1.3 200 grids (Electron Microscopy Sciences). The grids were blotted for 3s and plunge frozen into liquid ethane using a Vitrobot (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2019Quote: A protein sample at 1 mg/ml concentration was plunge-frozen on 200 mesh quantifoil R1.2/1.3 grids (Electron Microscopy Sciences) using a Mark IV Vitrobot (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: 3 μl of proteins (~0.05 mg/ml) diluted with PBS buffer were deposited onto carbon-coated grids (EMS CF200-Cu) glow-discharged for 15 seconds at 25 mA in air ...
-
bioRxiv - Biochemistry 2023Quote: ... the same proteins were applied to holey grids with a 2 nm carbon film (Quantifoil Cu 2/2 200 mesh from EMS). Samples were frozen after a total of 145 min on the grids without carbon film and 150 min on the grids with carbon film ...
-
bioRxiv - Genetics 2023Quote: ... Nucleic acid and protein staining were performed using 4% solutions of Uranyl Acetate (UA) (Electron Microscopy Sciences cat. 22400-4) and Phosphotungstic acid hydrate (PTA ...
-
bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 1 mg/ml (final for protein) and applied to freshly glow-discharged C-flat 300 mesh copper grids (CF-1.2/1.3, Electron Microscopy Sciences) at 4℃ ...
-
bioRxiv - Systems Biology 2023Quote: Mouse hearts were briefly perfused with 10ml cold PBS and then fixed with 4% paraformaldehyde (Electron Microscopy Sciences, Cat# 15710) (overnight ...
-
bioRxiv - Biophysics 2021Quote: The purified bison NHA2ΔN protein was individually applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences). Grids were blotted for 3.0 s or 3.5 s under 100% humidity and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2020Quote: ... Diluted S protein was mixed with a 1.5-fold molar excess of ACE2 and the mixture was incubated on ice for 1 minute before 4.8 uL of the protein mixture was deposited on a CF400-Cu grid (Electron Microscopy Sciences) before being stained with methylamine tungstate (Nanoprobes) ...
-
bioRxiv - Cell Biology 2021Quote: Exocyst holocomplex was mixed with liposomes composed of 95 mol% POPC and 5 mol% PI(4,5)P2 and protein A coated 5 nm gold fiducials (Electron Microscopy Sciences) for 3 minutes and to a final protein concentration of 1 μM ...
-
bioRxiv - Cell Biology 2022Quote: ... proteins in gels were transferred to nitrocellulose membranes and subsequently immobilized by 0.1% glutaraldehyde (15 min) (Electron Microscopy Sciences; Hatfield, PA). After blocking in Tris-Buffered Saline containing 0.1% Tween 20 (TBS-T ...
-
bioRxiv - Biochemistry 2020Quote: ... Cryo-EM grids were prepared by applying 3.5 μl of protein to the glow-discharged Quantifoil R1.2/1.3 200-copper mesh grids from Electron Microscopy Science (Q2100-CR1.3). The grids were blotted for 3 s ...
-
bioRxiv - Biochemistry 2021Quote: ... The sample was applied to glow discharged holey carbon grids which was then back blotted before adding 3 µL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
-
bioRxiv - Biochemistry 2021Quote: ... 3 μL protein solution at 0.01 mg/mL was added to a glow-discharged carbon-coated copper grid (Electron Microscopy Science). Grids were stained in 2% (w/v ...
-
bioRxiv - Biochemistry 2024Quote: ... Both sets of grids were then back blotted before adding 3 μL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
-
bioRxiv - Microbiology 2022Quote: ... with two-part conduction silver epoxy kit (EMS, 12642-14). For imaging ...
-
bioRxiv - Microbiology 2021Quote: ... with two-part conduction silver epoxy kit (EMS, 12642-14). For imaging ...
-
bioRxiv - Cell Biology 2022Quote: ... and Spurr low viscosity embedding kit (Electron Microscopy Sciences, 22050) was used to embed the VOs in resin ...
-
bioRxiv - Cell Biology 2023Quote: EMbed 812 Embedding Kit (Electron Microscopy Science, Cat no. 14121)
-
bioRxiv - Bioengineering 2023Quote: ... mouse tissues were perfused with 10 mL of cold PBS and 5mL of 4%paraformaldehyde (PFA) (Electron Microscopy Sciences, CAT# 15710). Brains were post-fixed overnight in 4% PFA at 4 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... A volume of 4 µl protein samples was applied to glow discharged Quantifoil R1.2/1.3 grids (Electron Microscopy Sciences, Hatfield, PA, USA) using a Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2020Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
-
bioRxiv - Neuroscience 2021Quote: ... and immediately placed on dry ice for protein or RNA extraction or fixed in fresh 4% paraformaldehyde (PFA, #1570-S, Electron Microscopy Science) for histological study ...
-
bioRxiv - Biochemistry 2021Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
-
bioRxiv - Molecular Biology 2021Quote: 4 µL of 0.05 mg/mL SARS-CoV-2 spike protein was applied onto a freshly glow discharged (1 min at 10 mA) CF300-Cu-50 grid (Electron Microscopy Sciences), incubated for 1 min ...
-
bioRxiv - Biochemistry 2022Quote: ... a 3-μl aliquot of the protein sample was applied to a glow-discharged Quantifoil holey carbon grid (Au 300 2/1 mesh) (Electron Microscopy Sciences), blotted for 2 s ...
-
bioRxiv - Cell Biology 2023Quote: ... The diluted sperm solution was mixed at a ratio of 1:1with 10 X concentrated 10 nm protein A-coated gold fiducials (Electron Microscopy Sciences). 2.7 μL of the mixture was applied onto the grids and blotted for 1.5 to 2.5 s in 100% relative humidity and 4 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... protein was diluted to a concentration of 25 nM and applied to CF400-copper ultra-thin support carbon grids (Electron Microscopy Sciences) that were glow discharged with a PELCO easiGlow (Ted Pella ...
-
bioRxiv - Neuroscience 2022Quote: ... Silver enhancement (Aurion R-gent Silver enhancer kit, Electron Microscopy Sciences) was performed following the manufacturer’s directions ...
-
bioRxiv - Neuroscience 2023Quote: ... The resin was prepared from the Epon 812 kit (EMS, USA) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... and infiltrated with EPON resin (Electron Microscopy Sciences, EMbed 812 Kit): ascending resin:propylene oxide with pure resin overnight and again the next morning ...
-
bioRxiv - Cancer Biology 2023Quote: ... A Differential Quik III Stain Kit (Electron Microscopy Sciences, Hatfield, PA) was used to stain the cells trapped in the membrane or matrigel ...
-
bioRxiv - Neuroscience 2023Quote: ... samples were embedded with an EMBed-812 embedding kit (EMS, USA) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Immuno-gold was modeled at the same size as secondary gold (6-nm anti-mouse and 10-nm anti-rabbit, Electron Microscopy Sciences) and colored similarly to the closest membrane as indicated in the Figure Legends ...
-
bioRxiv - Cell Biology 2023Quote: ... labeled with a secondary anti-rabbit or anti-mouse gold (depending on the primary antibody used) at RT for 1 hour (1:200 goat-anti-rabbit or goat-anti-mouse, Electron Microscopy Sciences), washed in 1% PBS with 3 sequential 50 µL drops ...
-
bioRxiv - Bioengineering 2023Quote: ... the grids were incubated in a drop of Goat-anti-Mouse IgG coupled with 10 nm gold nanoparticles (Electron Microscopy Sciences, 25512) diluted at 1:100 in PBS containing 0.1% BSA for 1 h ...
-
bioRxiv - Biochemistry 2023Quote: ... the protein samples were diluted to 0.05 mg/mL in their respective dialysis buffers and 3.5 μL of the protein samples were applied to freshly glow-discharged formvar-reinforced carbon grids (EMS: FCF-200-AU-EC), washed with water ...
-
bioRxiv - Immunology 2023Quote: ... sections prepared from mouse lungs frozen in OCT were stained with Sirius Red (0.1% Sirius Red in saturated picric acid, Electron Microscopy Sciences, 26357-02) for 60 min followed by a 2-min 0.01 N HCl wash ...
-
bioRxiv - Plant Biology 2024Quote: ... embedded using a Quetol 651 - NSA Kit (no. 14640, Electron Microscopy Sciences), and polymerized at 60°C ...
-
bioRxiv - Bioengineering 2022Quote: ... Then the sample was incubated with cleavage solution (EMS of the MiSeq kit) at 37°C for 3 × 15 min ...
-
bioRxiv - Cell Biology 2022Quote: ... and embedded in epoxy resin (Embed 812 kit for Electron Microscopy, EMS Company) using standard preparative procedure for ultrastructural analyses of tissues and cells ...
-
bioRxiv - Neuroscience 2022Quote: ... and finally embedding using the Technovit 7100 kit (Electron Microscopy Sciences Cat# 14653). Briefly ...
-
bioRxiv - Neuroscience 2022Quote: Postnatal mouse were transcardially perfused with 15ml (P14) or 25ml (>P21) of 2% PFA freshly prepared from 32% PFA solution (Electron Microscopy Sciences, 50-980-495). Perfused brains were dissected out ...
-
bioRxiv - Cancer Biology 2022Quote: ... and embedded in resin mixture of EMbed 812 Kit (Electron Microscopy Sciences cat#: 14120), cured in a 60°C oven ...
-
bioRxiv - Zoology 2022Quote: ... Samples were embedded with Spurr's Low Viscosity Embedding Media Kit (Electron Microscopy Sciences #14300), following the “Firm” formulation provided by the manufacturer ...