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Citations for Electron Microscopy Sciences :
51 - 100 of 110 citations for L Cysteinesulfinic acid monohydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: TSCs and TGCs were grown on poly-L-lysine–coated #1.5 glass coverslips (Electron Microscopy Sciences, Hatfield, PA) for confocal microscopy ...
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bioRxiv - Bioengineering 2022Quote: ... followed by approximately 1 L of 4% (w/v) paraformaldehyde (PFA) fixative (19208, Electron Microscopy Sciences, Hatfield, PA) in 1X PBS.
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bioRxiv - Developmental Biology 2023Quote: ... was used to counterstain sections and coverslips were mounted using Fluorogel l (Electron Microscopy Sciences, Hatfield, PA, USA). Confocal Microscopy image acquisition was taken on a LSM 710 microscope (Zeiss ...
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bioRxiv - Molecular Biology 2023Quote: ... 3μL of protein was deposited on holey carbon copper grids (QUANTIFOIL R 1.2/1.3-2nm, 300 mesh, EMS) which had been glow-discharged for 20s at 15 mA using PELCO easiGlow (TedPella Inc) ...
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bioRxiv - Neuroscience 2023Quote: ... A small longitudinal incision was then made using a diamond dissecting blade (Type M-DL 72029 L; EMS) along the glue line adjacent to the neuron of interest ...
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bioRxiv - Cell Biology 2021Quote: ... 2 mg/mL tannic acid and 0.5 mg/mL saponin in HKB) for 40 min on a thermanox plastic coverslip (EMS, USA), followed by washing in HKB buffer and then water ...
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bioRxiv - Microbiology 2020Quote: ... and stained at room temperature for 1 hour with 1% (wt/vol) low-molecular weight tannic acid (Electron Microscopy Science) in 01 ...
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bioRxiv - Molecular Biology 2023Quote: ... The output list of CandiSNP was screened for nonsynonymous amino acid changes and the G/C to A/T transitions that were likely to be caused by EMS, with a special focus on the chromosome with the highest SNP density with an allele frequency of 1 ...
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bioRxiv - Genetics 2023Quote: ... Nucleic acid and protein staining were performed using 4% solutions of Uranyl Acetate (UA) (Electron Microscopy Sciences cat. 22400-4) and Phosphotungstic acid hydrate (PTA ...
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bioRxiv - Plant Biology 2023Quote: ... The output list of CandiSNP was screened for non-synonymous amino acid changes and the G/C to A/T transitions that were likely to be caused by EMS, with a particular focus on the chromosome with the highest density of SNPs with an allele frequency of >0.75.
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bioRxiv - Plant Biology 2021Quote: ... The roots of 7 to 10 day old seedlings were collected and fixed for 30 min with 2-(N-morpholino) ethane sulfonic acid (MES) buffer solution pH 5.7 containing 4% paraformaldehyde (32% methanol-free solution; Electron microscopy Sciences). pH was adjusted to 5.7 with KOH solution.
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bioRxiv - Cell Biology 2021Quote: ... cells were stained with 500 μM nucleic acid intercalator iridium (191Ir and 193Ir, Fluidigm) in PBS with 1.6% PFA (Electron Microscopy Sciences) for 1 h at room temperature or overnight at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then plated as attached OPCs on 0.01% Poly-L-Lysine coated plates (Electron Microscopy Sciences, 19320-B) in the same media ...
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bioRxiv - Cell Biology 2019Quote: HEK293 Flp-ln T-REx cells were grown on poly-L-lysine-coated #1.5 glass coverslips (Electron Microscopy Sciences). Cells were treated with 1 μg/mL doxycycline for 24 h to induce expression of BirA* or BirA*-CNAα ...
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bioRxiv - Cell Biology 2019Quote: ... HeLa cells were grown on 22mm glass coverslips (#1.5) pre-treated with 0.01% poly-L-lysine (Electron Microscopy Sciences) and then coated with 10μg/ml laminin (Cultrex ...
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bioRxiv - Cell Biology 2021Quote: ... donkey anti-mouse IgG (H&L) conjugated with 15-nm gold particles (Electron Microscopy Science Aurion #25817, PA, USA).
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bioRxiv - Bioengineering 2022Quote: ... the first microfluidic device was placed on a clean poly-L-lysine coated slide (PLL slide, Electron Microscopy Science) and baked for 2 hours at 75°C oven to strengthen the bonding ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells plated on glass bottom dishes and platelets immobilized on poly-L-lysine (Electron Microscopy Sciences, Hatfield, PA)-coated on glass bottom dishes were fixed with 4.0 % paraformaldehyde (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 100 μL of the collected hemolymph was applied to poly-L-lysine slides (Electron Microscopy Sciences, Hatfield, PA, USA) and allowed to rest for ten minutes at room temperature on a flat surface (Fig ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 μL of the collected hemolymph was applied to poly-L-lysine slides (Electron Microscopy Sciences, Hatfield, PA, USA) and allowed to rest for ten minutes at room temperature on a flat surface ...
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bioRxiv - Biophysics 2021Quote: ... at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde, 2% paraformaldehyde, and 0.1% tannic acid prepared in 0.1 M phosphate buffer, pH 7, 15720, Electron Microscopy Sciences, Inc., PA). We washed the cells once with 0.1 M pH 7.2 phosphate buffer ...
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bioRxiv - Microbiology 2023Quote: ... The buffer was removed and samples stained with post-fixative for 3 hours (Osmium tetroxide, Ted Pella; Tannic Acid, Electron Microscopy Sciences). After washing with distilled water ...
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bioRxiv - Biochemistry 2020Quote: 5 μL of purified sample was applied to glow-discharged (10s) Quantifoil R 1.2/1.3 grids (Au 400; Electron Microscopy Sciences) and plunge-frozen in liquid nitrogen-cooled liquid ethane ...
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bioRxiv - Cell Biology 2021Quote: ... Washing and staining was performed by preparing a piece of parafilm with two 15-μL droplets of 1× folding buffer and two 15-μL droplets of 2% aqueous uranyl formate (electron microscopy sciences (EMS)) stain solution ...
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bioRxiv - Immunology 2021Quote: ... A 4-μl drop of the diluted sample was applied to a freshly glow-discharged carbon-coated copper grid (400-mesh, EMS) for approximately one minute ...
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bioRxiv - Biophysics 2023Quote: ... 2.5 μl of ∼3 mg/ml BmrCD was placed onto the Quantifoil UltrAuFoil grids (R1.2/1.3, 300 mesh, Electron Microscopy Sciences) which were glow discharged for 20 sec at 25 mA ...
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bioRxiv - Biochemistry 2023Quote: ... 3 µl of 1 mg/ml 70S ribosome RafH complex was applied on a glow discharged 300 CF300-Cu grids (EMS), the excess sample was blotted ...
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bioRxiv - Bioengineering 2023Quote: 5 μL of D-GQDs/exo loaded on the Poly-L-Lysine coated slides (63410; Electron Microscopy Sciences, PA, USA) and was fully covered with 18 mm × 18 mm square cover glasses (2845-18 ...
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bioRxiv - Immunology 2023Quote: ... sections prepared from mouse lungs frozen in OCT were stained with Sirius Red (0.1% Sirius Red in saturated picric acid, Electron Microscopy Sciences, 26357-02) for 60 min followed by a 2-min 0.01 N HCl wash ...
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bioRxiv - Cell Biology 2020Quote: ∼5-6μl of the resuspended 100,000 ×g pellet fraction was spread onto glow discharged Formvar-coated copper mesh grids (Electron Microscopy Sciences) and stained with 2% Uranyl acetate for 2 min ...
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bioRxiv - Cell Biology 2021Quote: Secondary antibodies: donkey anti-rabbit IgG (H&L) conjugated with 25-nm gold particles (Electron Microscopy Science Aurion #25708, PA, USA), donkey anti-mouse IgG (H&L ...
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bioRxiv - Microbiology 2020Quote: ... purified EVs were resuspended in 30 μL D-MEM (0% FCS) and mixed with a low volume of Glutaraldehyde 25% (final concentration = 2.5%) (16220, Electron Microscopy Sciences). After 10 min of fixation at RT ...
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bioRxiv - Cell Biology 2019Quote: ... The grids were blocked again for 1 h at room temperature and incubated for 2 h with 1:20 Goat-Anti-Rabbit IgG antibodies (H+L) (EMS 25104) and 1:10 Goat-Anti-Mouse IgG (H+L ...
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bioRxiv - Cell Biology 2022Quote: ... For immunofluorescence analysis cells were seeded (1.5 x 105 HeLa cells) directly on poly-L-lysine coated coverslips (Electron Microscopy Sciences), which were placed in a 12-well plate ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were euthanised with an overdose of MS-222 (1g/L in seawater) and fixed in 4% paraformaldehyde (Electron microscopy sciences) as per Gillis et al ...
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bioRxiv - Biophysics 2023Quote: Four μL of 5 μM GLS2 were applied to plasma-cleaned grids (300 mesh UltraAu Foil R1.2/1.3, Electron Microscopy Sciences, EMS) for a 30-second incubation ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-rabbit IgG (H+L) Ultrasmall EM grade 0.8nm colloidal gold (1:100 IG; Aurion, Electron Microscopy Sciences Cat# 25101).
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bioRxiv - Cell Biology 2024Quote: A drop (1-2 μl) of undiluted EVs (PEG/SEC-enriched EVs from 100 uninjured egfp(RNAi) animals) was placed on a formvar (EMS 15810)-coated hexagonal grid (EMS G300-Cu ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then washed thrice with PBS and smear dried on a Poly-L-lysine coated slide (Electron Microscopy Science: 63410), mounted with Prolong Diamond Antifade Mountant (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... fixed in 2 % PFA and allowed to attach on poly-L-lysine-coated 12 mm glass coverslips (Electron Microscopy Sciences, Hatfield, Pennsylvania) for 30 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... sectioned (thickness of 7-10 µm) and put at the center of poly-L-lysine covered glass slides (63478-AS, Electron Microscopy Sciences).
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bioRxiv - Biochemistry 2020Quote: ... An aliquot (approximately 4 μL) of diluted sample was placed on an ultra-thin carbon-coated 200-mesh copper grid (CF200-Cu-UL, Electron Microscopy Sciences, Hatfield ...
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bioRxiv - Cell Biology 2021Quote: ... The grids were washed with two 20 μL drops of ultrapure water and then stained with two 20 μL drops of 1% (w/v) uranyl acetate (Electron Microscopy Sciences); the first for 10 sec and the second for 1 min ...
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bioRxiv - Cell Biology 2022Quote: ... Peptide fibrils were obtained as described a ove and 5 μl of the sample was spotted on non-glow discharged carbon film 300 mesh copper grids (CF300-CU from Electron Microscopy Sciences) and incubated for 1 min ...
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bioRxiv - Biochemistry 2019Quote: ... were prepared by applying 3 – 5 μL of in vitro assembled PLP (with or without PC portal rings) onto 300-mesh carbon coated copper grids (Electron Microscopy Sciences). Samples were absorbed for 1 minute and washed with 2-3 drops of water ...
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bioRxiv - Biophysics 2020Quote: 4 μl of 22 μM tau aggregation samples were adsorbed onto glow-discharged 200 mesh formvar carbon coated copper grids (Electron Microscopy Sciences) and stained with aqueous uranyLess (Electron Microscopy Sciences) ...
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bioRxiv - Microbiology 2022Quote: Purified eCIS samples were briefly centrifuged to remove impurities and 5 μL of supernatant was applied onto freshly glow discharged carbon-coated copper grids (CF400-CU, 6 nm, 400 mesh, Electron Microscopy Sciences). Samples were allowed to adsorb for 2 minutes before being blotted off by filter paper and grids were washed twice with filtered distilled water ...
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bioRxiv - Microbiology 2023Quote: ... Sample aliquots (40 µl) were taken at regular time points over 40 h and fixed with 10 µl of 1% glutaraldehyde each (Electron Microscopy Sciences). The number of food vacuoles in 10 cells was counted under light microscopy ...
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bioRxiv - Bioengineering 2023Quote: A 80 × 50 × 20 mm (L × W × T) block of PMMA (Acrylic Plastic 24210-08, Electron Microscopy Sciences, Hatfield, PA 19440, USA) was cast and cured in a mold for 24 hours ...
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bioRxiv - Cell Biology 2022Quote: 10 μl of concentrated Pseudotyped SARS-CoV-2 S lentiviral particles were incubated for 30 min on carbon coated grids (EMS #CF200-CU). Excess solution was removed by back blotting ...