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Citations for Electron Microscopy Sciences :
51 - 100 of 1124 citations for 2 Amino 3 chloro 5 nitro 6 picoline since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... culture aliquots were taken and mixed 1:2 with Paraformaldehyde 3% Glutaraldehyde 0.35% In 0.1M Sodium Cacodylate Buffer pH 7.4 (Electron Microscopy Sciences). Cells were fixed for 30-60 min at RT and diluted in low fluorescence media ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 µl sample (5 x diluted) was placed to freshly glow-discharged carbon EM grid (400 mesh copper, Electron Microscopy Sciences). After incubating for 2-minute ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then washed 3 × 5 minutes in PBS and mounted to slides using FluoroGel with DABCO (Electron Microscopy Sciences, #17985) and 1.5 thickness coverglass (Fisherbrand ...
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bioRxiv - Microbiology 2024Quote: Clinical samples and biofilm samples fixed using the SEM fixation buffer were subsequently subjected to a series of washing steps (3 repetitions for 5 mins each) with 0.1M Cacodylate buffer pH 7.4(Electron Microscopy Sciences, USA) and distilled water (Gibco) ...
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bioRxiv - Biophysics 2024Quote: ... This cell suspension (3 µl) was applied to Cflat-2/1 200 mesh copper or gold grids (Electron Microscopy Sciences) that were glow discharged for 30 seconds at 15 mA ...
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bioRxiv - Plant Biology 2023Quote: Roughly 30,000 Arabidopsis bak1-5 bkk1-1 cerk1-2 (bbc) seeds were mutagenized using 0.2% ethyl methanesulfonate (EMS). Mutagenized M1 seeds were sown on soil and allowed to grow to set seeds ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then negatively stained for 1 min with 5 μL of 2% uranyl acetate (Electron Microscopy Sciences, 541-09-3 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated in 4’,6-diamidino-2-phenylindole (DAPI) and mounted onto slides with Fluoro-Gel fluorescence mounting medium (Electron Microscopy Sciences Cat# 1798502). Expression was observed via confocal microscopy.
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bioRxiv - Physiology 2024Quote: ... The sections were cover-slipped in Fluoromount-G™ Slide Mounting Medium with DAPI (4′,6- diamidino-2-phenylindole; Electron Microscopy Sciences, Hatfield, PA) and examined under a brightfield microscope to identify irregularities in the luminal surface in eTNAP mice ...
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bioRxiv - Physiology 2021Quote: The mouse liver was cut into 2-3 mm3 cubes and immediately immersed in a fixation solution containing 2.5% glutaraldehyde (Electron Microscopy Science) in 0.1 M cacodylate buffer pH 7.4 for 1 hour at room temperature and then overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of sample was applied to glow-discharged (30 s) Quantifoil copper grids (R2/2, 200 mesh, Electron Microscopy Sciences), blotted on both sides for 4 s ...
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bioRxiv - Microbiology 2023Quote: ... Ileum pieces were rinsed 3 times for 5 minutes in PB buffer and then postfixed with a fresh mixture of osmium tetroxide 1% (EMS, Hatfield, PA, US) with 1.5% potassium ferrocyanide (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... the samples were rinsed 3 times for 5 min each in PB buffer and postfixed with a fresh mixture of 1% osmium tetroxide (EMS, Hatfield, PA, US) and 1.5% potassium ferrocyanide (Sigma ...
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bioRxiv - Genetics 2021Quote: ... 3 μl of the diluted 3C library was deposited onto a plasma-clean Quantifoil R2/2 holey carbon grid (Electron Microscopy Sciences), blotted with a filter paper for 2 seconds ...
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bioRxiv - Neuroscience 2021Quote: ... PCW18 and PCW19 spinal cords were dissected out of the vertebral column and fixed for 2-3 hours at 4°C with 4% paraformaldehyde (PFA, Electron Microscopy Sciences), washed with PBS and transferred to a 30% sucrose solution for 48-72 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then fixed using Karnovsky Fixative Reagent Grade (3% glutaraldehyde and 2% formaldehyde in 0.1 M phosphate buffer, pH 7.4) (Electron Microscopy Sciences, 15732-10) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... was perfused with 10 mL of 1x PBS followed by 30 mL of fixative 2% PFA/0.075% GA in PBS (32% PFA Alfa Aesar, 3% GA Electron Microscopy Science). Group 2 (indirect PFA ...
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bioRxiv - Genetics 2023Quote: ... pH 6 (64142-07, Electron Microscopy Sciences). The tissue sections were then incubated in the Click-iT Edu Reaction Cocktail for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... 002% NaN3, and 2 mM EDTA in PBS 1X) and centrifuged (500 × g, 5 min) before adding paraformaldehyde (PFA, EMS) at a final concentration of 16% for 10 min at room temperature in FCB ...
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bioRxiv - Immunology 2020Quote: ... slides were washed twice with MIBI wash buffer and fixed for 5 min in 2% glutaraldehyde solution (Electron Microscopy Sciences) in low-barium PBS ...
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bioRxiv - Neuroscience 2024Quote: ... using a Leica ARTOS 3D ultramicrotome, 2-3 74 nm thick sections were collected (4 levels, ∼ 8-10 μm apart) on copper grids (EMS, cat# G150-Cu) and imaging on TEM - 120kv JEOL 1400-Flash equipped with a 4k Gatan OneView digital camera ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were washed twice for 5 minutes in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
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bioRxiv - Immunology 2020Quote: ... slides were washed twice for 5 minutes in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
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bioRxiv - Systems Biology 2023Quote: ... slides were washed twice for 5 minutes in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
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bioRxiv - Immunology 2024Quote: ... slides were washed twice for 5 min in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low- barium PBS for 5 min ...
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bioRxiv - Immunology 2024Quote: ... slides were washed twice for 5 min in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... and 1.5% K3Fe(CN)6 (EMS, Hatfield, USA) at 4°C followed by a contrasting step with 1% thiocarbohydrazide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... and then perfused with RPMI media containing 5 U/mL heparin (Sagent Pharmaceuticals, Cat. #NDC 25021-400-10) followed by 3% paraformaldehyde (PFA) (Electron Microscopy Science, Cat. # 15714-S) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... the grid was stained one time for 3 minutes and three times for 1-minute each using 2% uranyl acetate (Electron Microscopy Sciences, Hatfield, PA). Excess stain was wicked away in between the steps ...
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bioRxiv - Genetics 2022Quote: ... Dissected gonads were fixed in 2 mL 3% paraformaldehyde (PFA) (10 mL 16% PFA, EM Grade, Electron Microscopy Sciences, Hatfield, PA Catalog No 15710) phosphate-buffered solution for 10 minutes at room temperature and then post-fixed with 2 mL 100% methanol (Gold-label from Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... 3% paraformalydehyde (15754-S, EMS) and 0.1 M Sorensen’s phosphate buffer pH 7.2 solution ...
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bioRxiv - Neuroscience 2023Quote: ICC(3, 1) = (BMS-EMS)/(BMS+(k-1)*EMS),
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bioRxiv - Biophysics 2021Quote: ... at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde, 2% paraformaldehyde, and 0.1% tannic acid prepared in 0.1 M phosphate buffer, pH 7, 15720, Electron Microscopy Sciences, Inc., PA). We washed the cells once with 0.1 M pH 7.2 phosphate buffer ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for 10-15 minutes at 4 °C before fixation in 2% paraformaldehyde solution for 5 minutes (Electron Microscopy Sciences). In the case of intracellular staining for TCRζ ...
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bioRxiv - Neuroscience 2022Quote: ... was performed over the vS1 of the mouse and covered with a double-layered round glass coverslip (3 mm: 64-0720 [CS-3R], Warner Instruments, Hamden, CT; 5 mm: Electron Microscopy Sciences, Hatfield, PA, 72195-05), bonded with an optical adhesive (NOA71 ...
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bioRxiv - Cell Biology 2023Quote: ... with red anti-Alfa FluoTag for 30 min before being washed 3 times with PBS and fixed with 2% methanol-free PFA (Electron Microscopy Sciences, cat#157-8). Coverslips were directly mounted using Prolong Diamond Antifade mounting medium (ThermoFischer ...
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bioRxiv - Biophysics 2024Quote: ... the cells were fixed for 10 minutes in PEM buffer (80 mM PIPES, 5 mM EGTA, 2 mM MgCl2) containing 4% formaldehyde (FA) (32% sol. in glass ampoules, Electron Microscopy Sciences, #50980494) with 4% sucrose (Sigma ...
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bioRxiv - Cell Biology 2024Quote: Primary neurons or astrocytes cultured on glass coverslips at the same plating density as was used for live cell imaging experiments were fixed for 10 minutes at 37°C/5% CO2 in a solution containing 2% paraformaldehyde (PFA; Electron Microscopy Sciences 15713) and 0.05% glutaraldehyde in PHEM buffer (EMS ...
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bioRxiv - Neuroscience 2023Quote: ... a cylinder of tissue 2 mm in diameter and 3 mm in height for each area was collected with a tissue punch (EMS-core sampling tool, 69039-20, EMS), and ejected into PCR tubes filled with 100 µL of RNAlater and kept at −80°C for long-term storage ...
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bioRxiv - Physiology 2024Quote: ... and 3% lead citrate (22410, EMS) for 10 minutes ...
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bioRxiv - Genomics 2022Quote: ... 5% fixation buffer [5% paraformaldehyde (EMS, cat. no. 50-980-493), 1.25x dPBS] was added to each well ...
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bioRxiv - Plant Biology 2023Quote: ∼6 000 seeds were mutagenized using 0.4 % ethyl methanesulfonate (EMS) in 100 mM phosphate buffer ...
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bioRxiv - Microbiology 2021Quote: ... After that, Samples were infiltrated in graded mixtures (1:3, 1:1, 3:1) of resin (EMS, Resin Mixture ...
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bioRxiv - Biochemistry 2024Quote: CryoEM grids were prepared by applying 3.5 μL of 0.6 mg/mL NX11Q21.5-DNA48 in TBS with 2.5% glycerol to glow-discharged 400 mesh C-flat grids (CF-2/2-4C, 2 μm holes, 2 μm spacing; Electron Microscopy Sciences). The grids were blotted with a blot force of 0 for 6.5 seconds at 100% humidity and 20 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated 2 min on 2% paraformaldehyde (Electron Microscopy Sciences), then incubated for 30 sec on 2% uranyl acetate (aqueous ...
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bioRxiv - Molecular Biology 2023Quote: ... The output list of CandiSNP was screened for nonsynonymous amino acid changes and the G/C to A/T transitions that were likely to be caused by EMS, with a special focus on the chromosome with the highest SNP density with an allele frequency of 1 ...
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bioRxiv - Plant Biology 2023Quote: ... The output list of CandiSNP was screened for non-synonymous amino acid changes and the G/C to A/T transitions that were likely to be caused by EMS, with a particular focus on the chromosome with the highest density of SNPs with an allele frequency of >0.75.
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bioRxiv - Biophysics 2022Quote: ... CF-2/2-4C (CF-224C-100) (Electron Microscopy Sciences) cryoEM grids ...
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bioRxiv - Neuroscience 2021Quote: ... 2% paraformaldehyde (EMS) in 0.1 M sodium cacodylate buffer (EMS) ...
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bioRxiv - Neuroscience 2021Quote: ... 2% glutaraldehyde (EMS), 2.2mM CaCl2 diluted in 50 mM MOPS buffer (pH 7.4 ...