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Citations for Electron Microscopy Sciences :
51 - 100 of 1463 citations for 1 3 Nitro 10 11 dihydro 5H dibenzo b f azepin 5 yl ethanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2024Quote: ... infiltrated with 50%/50% propylene oxide/epon medium hard formulation (EMbed 812, Electron Microscopy Sciences; [11]), and then 100% epon medium hard ...
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bioRxiv - Developmental Biology 2024Quote: ... for nuclear antigens and R-buffer B (Electron Microscopy Sciences) for cytoplasmic antigens ...
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bioRxiv - Bioengineering 2022Quote: ... The dehydrated beads were immersed in 3/1 acetone/Embed 812 (Electron Microscopy Sciences) for 12 hr ...
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bioRxiv - Plant Biology 2024Quote: ... 4 µL of this sample at 2 µg Chl mL−1 was applied to a glow discharged (60 s, 25 mA) 400 mesh Cu grid with 5-6 nm formvar with 3-4 nm carbon (Electron Microscopy Sciences). The sample was negatively stained with 2% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... FFPE sections (3 um) were collected and underwent antigen retrieval in a citrate (pH 6.0, Electron Microscopy Sciences, 62706-10) or Tris-EDTA (pH 9.0 ...
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bioRxiv - Cell Biology 2021Quote: Samples were fixed in mixture of 4% paraformaldehyde (TAAB Laboratories Equipment Ltd, United Kingdom, cat. F/P001) and 1% glutaraldehyde (Electron Microscopy Science, USA, cat. 16310) in 0.1 M phosphate buffer (pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was applied to glow discharged holey carbon grids which was then back blotted before adding 3 µL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Biochemistry 2024Quote: ... Both sets of grids were then back blotted before adding 3 μL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Neuroscience 2024Quote: ... 1–3-day-old whole adult flies were fixed in 4% paraformaldehyde (Electron Microscopy Sciences) in PBST (PBS containing 1% Triton X-100 ...
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bioRxiv - Neuroscience 2023Quote: ... for 5 min and negative staining was performed with 1% uranyl acetate (EMS) for 1 min.
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bioRxiv - Bioengineering 2020Quote: ... were prepared by applying 3 µL of 10 mg/mL sample on pretreated lacey carbon 400 mesh TEM grids (Electron Microscopy Sciences). Following a 3 s blot ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then fixed using Karnovsky Fixative Reagent Grade (3% glutaraldehyde and 2% formaldehyde in 0.1 M phosphate buffer, pH 7.4) (Electron Microscopy Sciences, 15732-10) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: Primed and naïve hESCs were prepared as described in the above section and fixed for 10 min in a 3% paraformaldehyde solution (Electron Microscopy Science). Cells were then permeabilized for 7 min with ice-cold PBS supplemented with 0.5% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... 3 mL of LBv2 media without substance or test conditions (EMS [1:1,000](Sigma-Aldrich, #M0880) or MMS [1:10,000](Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Then cells were manually unroofed by spraying 1 mL of 3% paraformaldehyde (Electron Microscopy Sciences #15710) and 1% glutaraldehyde (Electron Microscopy Sciences #16216 ...
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bioRxiv - Immunology 2024Quote: ... At -60 °C samples were infiltrated with increasing concentrations (30, 60, and 100% for 1 h each) of Lowicryl (3:1 K11M/HM20 mixture; Electron Microscopy Sciences). After 5 h of 100% Lowicryl infiltration ...
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bioRxiv - Neuroscience 2024Quote: ... using a Leica ARTOS 3D ultramicrotome, 2-3 74 nm thick sections were collected (4 levels, ∼ 8-10 μm apart) on copper grids (EMS, cat# G150-Cu) and imaging on TEM - 120kv JEOL 1400-Flash equipped with a 4k Gatan OneView digital camera ...
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bioRxiv - Neuroscience 2022Quote: ... 1 mM DTT) and fixed by the addition of 3 ml PBS 1.33% paraformaldehyde (Electron Microscopy Sciences) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2021Quote: ... 3% paraformalydehyde (15754-S, EMS) and 0.1 M Sorensen’s phosphate buffer pH 7.2 solution ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 30µl/ml of solution B (Cat no.-14270-04, Electron Microscopy Sciences, USA) was added to the JB4-dye mix ...
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bioRxiv - Plant Biology 2024Quote: BEEM capsules (Size 00, 8mm I.D, Electron Microscopy Sciences, Cat. No. 70000-B)
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bioRxiv - Cell Biology 2024Quote: ... stained with Weigert’s iron Haematoxylin A & B solutions (EMS; 26367-02 and −03), Biebrich scarlet solution (EMS ...
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bioRxiv - Neuroscience 2023Quote: ... 70-nm thick sections from these areas were generated (4-6 levels,[∼[5–10 µm apart) and collected on silicon wafers (EMS, Pennsylvania) for imaging on a Zeiss Crossbeam 350 focused ion beam-scanning electron microscope (FIB-SEM) ...
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bioRxiv - Neuroscience 2024Quote: ... transcardially perfused with PBS for 5 min and then with 10 mL of 4% PFA solution (Electron Microscopy Sciences, Cat#15714) for immunohistochemistry or with 10 mL of 2.5% glutaraldehyde (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... was performed over the vS1 of the mouse and covered with a double-layered round glass coverslip (3 mm: 64-0720 [CS-3R], Warner Instruments, Hamden, CT; 5 mm: Electron Microscopy Sciences, Hatfield, PA, 72195-05), bonded with an optical adhesive (NOA71 ...
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bioRxiv - Genetics 2022Quote: ... Dissected gonads were fixed in 2 mL 3% paraformaldehyde (PFA) (10 mL 16% PFA, EM Grade, Electron Microscopy Sciences, Hatfield, PA Catalog No 15710) phosphate-buffered solution for 10 minutes at room temperature and then post-fixed with 2 mL 100% methanol (Gold-label from Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... The diluted sperm solution was mixed at a ratio of 1:1with 10 X concentrated 10 nm protein A-coated gold fiducials (Electron Microscopy Sciences). 2.7 μL of the mixture was applied onto the grids and blotted for 1.5 to 2.5 s in 100% relative humidity and 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... and immediately crosslinked for 10 min in 1% formaldehyde (Electron Microscopy Sciences, #15710). ChIP assays were performed as reported (Amin et al ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 mM MgSO4 supplemented with 1% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA), 100 μM 3-maleimidobenzoic acid N-hydroxysuccinimide ester (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 mM MgSO4 supplemented with 1% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA), 100 µM 3-maleimidobenzoic acid N-hydroxysuccinimide ester (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2024Quote: ... 10 mM MgSO4) supplemented with 1% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA), 100 µM 3-maleimidobenzoic acid N-hydroxysuccinimide ester (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for 10-15 minutes at 4 °C before fixation in 2% paraformaldehyde solution for 5 minutes (Electron Microscopy Sciences). In the case of intracellular staining for TCRζ ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were stained with 10% Uranyl acetate (Sisco Research Laboratory Pvt. Limited, 81405) for 5-min and Sato’s lead citrate (Electron Microscopy Sciences, 17800) stain for 1-min ...
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bioRxiv - Cell Biology 2022Quote: ... Weigert’s iron hematoxylin A and B (26044-05 and 26044-15; Electron Microscopy Sciences), Biebrich Scarlet-Acid Fucshin (HT151 ...
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bioRxiv - Immunology 2024Quote: ... Coverslips were placed cell-side down on BEEM capsules (Electron Microscopy Sciences, #70010-B) filled with EPON ...
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bioRxiv - Developmental Biology 2024Quote: ... for nuclear antigens or Buffer B (Electron Microscopy Sciences, cat number 50-311-78) for cytoplasmic antigens using a pressure cooker ...
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bioRxiv - Physiology 2024Quote: ... and 3% lead citrate (22410, EMS) for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... culture aliquots were taken and mixed 1:2 with Paraformaldehyde 3% Glutaraldehyde 0.35% In 0.1M Sodium Cacodylate Buffer pH 7.4 (Electron Microscopy Sciences). Cells were fixed for 30-60 min at RT and diluted in low fluorescence media ...
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bioRxiv - Genomics 2022Quote: ... 5% fixation buffer [5% paraformaldehyde (EMS, cat. no. 50-980-493), 1.25x dPBS] was added to each well ...
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bioRxiv - Cancer Biology 2023Quote: ... The next day cells were detached using detachment buffer (0.05% EDTA in PBS) for 5 minutes and harvested in 10% HI-FBS DMEM and fixed with 4% paraformaldehyde (PFA, no-15710, Electron microscopy sciences, USA) in PBS for 10 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: Primary neurons or astrocytes cultured on glass coverslips at the same plating density as was used for live cell imaging experiments were fixed for 10 minutes at 37°C/5% CO2 in a solution containing 2% paraformaldehyde (PFA; Electron Microscopy Sciences 15713) and 0.05% glutaraldehyde in PHEM buffer (EMS ...
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bioRxiv - Genomics 2022Quote: ... biological specimens were transferred to microporous specimen capsules (30 μm porosity, 12 mm diameter, 11 mm high, ref #70187-20, Electron Microscopy Science) and dehydrated in a graded series of ethanol in double-distilled water (50 ...
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bioRxiv - Biophysics 2022Quote: ... The continuous carbon layer was first deposited on mica support of 25 x 25 mm (Cat Nr 71850-11, Electron Microscopy Science). The carbon layer was floated on the water surface and then transferred to the cryo-EM grids by raising them to the water surface under the carbon layer ...
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bioRxiv - Immunology 2024Quote: ... Cells were rinsed twice in FACS buffer then fixed in 1% PFA (Electron Microscopy Sciences, 15714-5) for 1 hr at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... 10 ml of retromer at concentrations between 5 and 10 μg/mL were applied to continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences, Hatfield, PA) and washed twice with water ...
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bioRxiv - Biochemistry 2021Quote: ... and 3 µL of sample was applied onto glow-discharged C-flat grids (EMS CF-1.2/1.3-4Au-1. 50) for 3 sec before plunge freezing in liquid ethane using a Leica EM Grid Plunger (Leica Microsystems) ...
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bioRxiv - Cell Biology 2021Quote: Organoids were fixed in 3% glutaraldehyde and 1% paraformaldehyde in 0.08 M sodium acodylate buffer (all from Electron Microscopy Sciences) overnight with gentle rocking at 4°C ...
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bioRxiv - Physiology 2024Quote: Isolated TALs and DCTs were embedded into 1% agarose and fixed in 3% formaldehyde/glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) for 24 hours ...
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bioRxiv - Biophysics 2024Quote: ... This cell suspension (3 µl) was applied to Cflat-2/1 200 mesh copper or gold grids (Electron Microscopy Sciences) that were glow discharged for 30 seconds at 15 mA ...
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bioRxiv - Neuroscience 2024Quote: ... Devices were fabricated by thoroughly mixing polydimethylsiloxane (PDMS) and a curing agent in a 10:1 ratio by weight (Sylgard 184, Electron Microscopy Sciences Cat. 24236 – 10), degassing the mixture under vacuum ...