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Citations for Electron Microscopy Sciences :
901 - 950 of 1733 citations for Acetamide N 2 3 dihydro 2 3 hydroxy 2 quinolinyl 1 3 dioxo 1H inden 5 yl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Cells were then washed with FACS buffer and once with PBS before fixing with 200 mL 2% paraformaldehyde (PFA; Electron Microscopy Sciences, Cat# 15710) in PBS overnight or longer ...
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bioRxiv - Biochemistry 2023Quote: ... 2.5mM Mg(OAc)2 wash buffer to an optical density of 2 A260/mL (corresponding to 40 nM of 80S) and deposited on copper grids (Electron Microscopy Sciences, CF400-CU). Grids were glow-discharged at 15 mA for 30s 4 uL of sample were incubated on the glow-discharged grids for 1min ...
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bioRxiv - Physiology 2024Quote: ... The sections were cover-slipped in Fluoromount-G™ Slide Mounting Medium with DAPI (4′,6- diamidino-2-phenylindole; Electron Microscopy Sciences, Hatfield, PA) and examined under a brightfield microscope to identify irregularities in the luminal surface in eTNAP mice ...
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bioRxiv - Neuroscience 2022Quote: Postnatal mouse were transcardially perfused with 15ml (P14) or 25ml (>P21) of 2% PFA freshly prepared from 32% PFA solution (Electron Microscopy Sciences, 50-980-495). Perfused brains were dissected out ...
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bioRxiv - Neuroscience 2021Quote: ... cultures were washed in imaging buffer to remove non-internalised HRP and fixed with a solution of 2 % glutaraldehyde (Electron Microscopy Sciences, Hatfield, USA, 16019) in phosphate buffered saline ...
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bioRxiv - Neuroscience 2023Quote: ... cultures were washed in imaging buffer to remove non-internalised HRP and fixed with a solution of 2 % glutaraldehyde (Electron Microscopy Sciences, Hatfield, USA, 16019) and 2% PFA in 0.1 M in phosphate buffer (PB) ...
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bioRxiv - Neuroscience 2023Quote: ... isoflurane overdose was used to deeply anesthetize mice and cold PBS was transcardially perfused for 2 minutes followed by 7 minutes of perfusion with 4%PFA freshly made from 32% (Electron Microscopy Sciences Cat #15714-S) at a rate of 7 mL/min ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were fixed for at least 2 hours (at room temperature) to overnight (at 4° C) in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) in artificial sea water ...
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bioRxiv - Neuroscience 2022Quote: ... tissue was dehydrated through an ethanol series (70% ethanol, 90% ethanol, 2 x 100% ethanol; 8 minutes each) and mounted in Entellan (Electron Microscopy Science, Hatfield, PA, USA).
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bioRxiv - Neuroscience 2024Quote: mice were perfused 7 dpI and 10 dpl with 25 mL of 2% PBS-paraformaldehyde freshly prepared from 32% PFA solution (Electron Microscopy Sciences, 50-980-495). Perfused brains were dissected out ...
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bioRxiv - Neuroscience 2024Quote: ... 70 nm ultrathin sections were taken and post-stained with 2% aqueous uranyl acetate and lead citrate (22400, 22405, 17800, 17900, 21140, Electron Microscopy Sciences, Hatfield, PA, USA). Samples were imaged on a TEM (Jeol JEM-1400 Plus ...
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bioRxiv - Cell Biology 2021Quote: Cells were cultured on cell-tight C-2 cell disks (Sumitomo Bakelite, Tokyo, Japan; #MS-0113K) and fixed for 2 h on ice in 2.5% glutaraldehyde (Electron Microscopy Science, Hatfield, PA; #111-30-8) in 0.1 M phosphate buffer ...
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bioRxiv - Pathology 2021Quote: ... Sections were stained with hematoxylin and eosin (H&E; Fisherbrand™ 517-28-2, Waltham, MA) or safranin-O and fast green (Saf-O; Electron Microscopy Sciences® 20800, Hatfield, PA), following manufacturer protocols ...
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bioRxiv - Neuroscience 2023Quote: ... 3.5 μL of CopiaGag capsid protein sample was applied to a 300-mesh copper grid with continuous 2 nm carbon film (Electron Microscopy Sciences, QUANTIFOIL® C2-C15nCu30-50) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The sample attached to the grid was stained by immediately tapping the grid on 2% w/w uranyl acetate (Electron Microscopy Sciences, Hatfield, PA, USA, Cat. No. 22400) solution droplets twice ...
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bioRxiv - Developmental Biology 2022Quote: ... MGs were stained for 1h in Oil Red O (ORO) solution (Electron Microscopy Sciences, Hatfield, PA) at room temperature (RT) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We stained them en bloc with 1% aqueous uranyl acetate (Electron Microscopy Sciences, cat n. 22400) for 16 hours at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... 5 μL of 1% uranyl acetate in water (Electron Microscopy Sciences 22400-1) was added to the grid ...
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bioRxiv - Neuroscience 2021Quote: ... retinal sections were fixed with glutaraldehyde (2.5% in PBS at pH 7.4 for 1h at RT; Electron Microscopy Services-EMS), rinsed with PBS and then with double-distilled water ...
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bioRxiv - Cell Biology 2022Quote: ... Glutaraldehyde (Electron Microscopy Science, cat n°16220). The following primary antibodies were used in this study ...
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bioRxiv - Cell Biology 2021Quote: ... fixed for 5 min with 1% glutaraldehyde (Electron Microscopy Sciences).
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bioRxiv - Neuroscience 2022Quote: ... and a 5-mm-diameter #1 glass coverslip (Electron Microscopy Sciences) was placed over the window and sealed to a custom-made metal ring using cyanoacrylate and dental cement ...
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bioRxiv - Biophysics 2021Quote: A 10 μl volume of 5 times diluted aggregated sample (initial αS concentration = 50 µM and N-protein concentration = 0.2 – 1 μM) was deposited onto freshly cleaved mica (Muscovite mica, V-1quality, EMS, US) and left to rest for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... 4 mM NaHCO3, 1 mM NaH2PO4, 5 mM trehalose, 10 mM sucrose, 5 mM HEPES, pH 7.5, prepared by Electron Microscopy Sciences) and then bathed in whole organ ex vivo culture medium with 10 µg/ml isradipine (Selleck Chemical LLC ...
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bioRxiv - Microbiology 2021Quote: ... after receiving 1 μl of 5× concentrated 10 nm Au fiducial solution (EMS) in the back-side and being blotted from the back ...
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bioRxiv - Neuroscience 2023Quote: ... for 5 min and negative staining was performed with 1% uranyl acetate (EMS) for 1 min.
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bioRxiv - Neuroscience 2020Quote: ... Cat N° SND75 (microgroove length: 75 μm) and Cat N° XC150 (microgroove length: 150 μm)) and 7.8mil Aclar 33C sheets (Electron Microscopy Sciences, Hatfield, PA, USA, Cat N° 50425-25) were sterilized in 70% ethanol and left to air-dry in the laminar flow ...
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bioRxiv - Cell Biology 2022Quote: ... mouse eyeballs were fixed o/n in 4% (w/v) PFA (171010; EMS) at 4°C ...
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bioRxiv - Genomics 2022Quote: ... 5% fixation buffer [5% paraformaldehyde (EMS, cat. no. 50-980-493), 1.25x dPBS] was added to each well ...
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bioRxiv - Immunology 2024Quote: ... Cells were rinsed twice in FACS buffer then fixed in 1% PFA (Electron Microscopy Sciences, 15714-5) for 1 hr at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... washed and fixed O/N in 4% paraformaldehyde/phosphate buffered saline (PBS) solution (EMS). Left lung lobes were then treated for paraffin inclusion ...
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bioRxiv - Cancer Biology 2020Quote: Cells cultured on coverslips were fixed in 4% PFA (Electron Microscopy Sciences, Cat. N. 15710) in PBS for 10 min followed by incubation in blocking solution for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... cells were fixed with PFA 4% in PBS 1X (Electron Microscopy Sciences, cat n°15700) for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with ddH2O three times 5 min and incubated in 1% thiocarbohydrazide (EMS, cat# 2231-57-4) diluted in ddH2O for 20 min ...
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bioRxiv - Biochemistry 2024Quote: ... 5 μL of sample was applied to a glow discharged QF-R2/1 Cu 200M grid (Electron Microscopy Sciences). Grids were frozen into -185 °C liquid ethane using a Leica EM GP2 automatic plunge freezer equilibrated to 80% humidity and 10 °C with a 10 second sample incubation time and a 2.5 second blot time on Whatman 1 paper ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the chips were spiked with the CitiFluorTM AF1 mountant solution (Electron microscopy sciences, cat. N° 17970.25) before confocal imaging with ZEISS LSM880.
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bioRxiv - Biophysics 2021Quote: ... after which 5-μL were deposited in a glow-discharged Quantifoil grid (200-mesh copper R2/1, Electron Microscopy Sciences), followed by manual blotting and plunge freezing in a 50/50 ethane propane mix (Airgas ...
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bioRxiv - Biochemistry 2024Quote: ... AFM samples were prepared by adsorbing 10 μl of 5 times diluted sample onto mica (Muscovite mica, V 1 quality, EMS) for 45 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... fine-tipped forceps (Dumont #5, EMS). Holding the coverslip vertically ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM EDTA) and 5 μL of the mixture was applied to carbon-coated 300 mesh Cu grids (Electron Microscopy Sciences) after glow discharging for 1 min at −15 mA in an EasiGlo glow discharger (Ted Pella) ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μL of the EV sample was adsorbed for 1 min to a carbon-coated grid (# CF400-CU, Electron Microscopy Sciences) that had been made hydrophilic by a 20-sec exposure to a glow discharge (25mA) ...
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bioRxiv - Microbiology 2022Quote: ... LSUCC0664 was grown to 106 cells mL-1 and 5 µL of culture was loaded onto a glow discharged 300 mesh carbon filmed grid (EMS:CF300-cu). We removed excess liquid with filter paper after 2 minutes and stained with 2% uranyl acetate (TED Pella Cat ...
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bioRxiv - Microbiology 2024Quote: ... subtilis colonies were inoculated into 5 mL of LB medium supplemented with 5 µL of ethyl methanesulfonate (EMS), and cultured at 37°C overnight ...
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bioRxiv - Developmental Biology 2024Quote: ... Dumont tweezers (Style 5, Electron Microscopy Sciences) were used ...
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bioRxiv - Bioengineering 2022Quote: ... 5% normal donkey serum (Electron Microscopy Sciences) in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... and 5 mm (Electron Microscopy Sciences, 7229605) glass coverslips were prepared by plasma etching with oxygen for 1 minute (GLOW Plasma System ...
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bioRxiv - Cancer Biology 2024Quote: ... then resuspended in 60 µl PBS with 1% BSA and 5 µl of cells was added to each well of a multi-well microscope slide (Electron Microscopy Sciences) pre-coated with 0.1% poly-L-lysine (Sigma Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... and 5 mL of 4% Paraformaldehyde (PFA) (EMS) diluted in RNase free PBS ...
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bioRxiv - Neuroscience 2023Quote: Mice (female; n=4) at 15 dpl were perfused with PFA prepared in 0.1 M Sorensen’s buffer (Electron Microscopy Sciences). The spinal cords were harvested ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was added to the plates mentioned above and incubated for 1 h at 37°C with 5% CO2 after which cells were fixed using 4% PFA (Electron Microscopy Sciences, Hatfield, PA) and incubated at room temperature for 30 minutes ...