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Citations for Electron Microscopy Sciences :
801 - 850 of 854 citations for Integrin beta 1 binding protein 1 ITGB1BP1 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Four microliters of protein was loaded onto a glow-discharged CF200-CU carbon grid (Electron Microscopy Sciences, U.K.). After 5 min of incubation ...
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bioRxiv - Microbiology 2024Quote: ... A 3.5-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 s using a PELCO easiGlow Cleaning System (Ted Pella) ...
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bioRxiv - Neuroscience 2020Quote: ... Girds were immersed in 15µl beads of secondary antibody (Electron microscopy sciences, goat-anti-rabbit 15 nm ...
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bioRxiv - Cell Biology 2021Quote: Samples were fixed in mixture of 4% paraformaldehyde (TAAB Laboratories Equipment Ltd, United Kingdom, cat. F/P001) and 1% glutaraldehyde (Electron Microscopy Science, USA, cat. 16310) in 0.1 M phosphate buffer (pH 7.4 ...
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bioRxiv - Microbiology 2024Quote: ... This concentrate was diluted 1:2 with molecular grade water and 50 µL was fixed with 25% glutaraldehyde (stored at 4°C, Electron Microscopy Sciences, Hatfield, PA, USA) to a final concentration of 0.25% ...
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bioRxiv - Neuroscience 2024Quote: ... Devices were fabricated by thoroughly mixing polydimethylsiloxane (PDMS) and a curing agent in a 10:1 ratio by weight (Sylgard 184, Electron Microscopy Sciences Cat. 24236 – 10), degassing the mixture under vacuum ...
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bioRxiv - Biochemistry 2024Quote: ... 4 µl of sample were added to freshly glow-discharged 300 mesh 2/1 µm C-flat copper grids (Electron Microscopy Sciences, Hatfield, Pennsylvania, USA), blotted for 10 s at blot force 4 at 100% relative humidity and 4°C before being plunge frozen in a liquid mix of 37% (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... 4 μL of the protein solution was applied to a glow-discharged Formvar-carbon grid (EMS FCF200-Cu-UB) for 1 min followed by blotting ...
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bioRxiv - Biophysics 2023Quote: A volume of 3.5 μl of purified channel protein was applied onto glow-discharged copper holey carbon grids R1.2/1.3 (Q350CR1.3, Electron Microscopy Sciences), which was cleaned for 1 minute in an H2/O2 plasma using a Gatan Solarus 950 (Gatan) ...
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bioRxiv - Molecular Biology 2022Quote: 3 μl of protein solution were applied to glow-discharged carbon coated copper grid (300 mesh, Electron Microscopy Science) and let adsorb for 30 s ...
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bioRxiv - Immunology 2024Quote: ... A 3.5-μL drop of protein was deposited on a Quantifoil Au-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 40 seconds using a PELCO easiGlow Cleaning System (Ted Pella) ...
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bioRxiv - Plant Biology 2020Quote: ... After 1 hour the leaf discs were washed three times with sterile water and then mounted in Fluoromount-G ™ (Electron microscopy Sciences, Hatfield, PA, USA). Leaf discs were imaged with a Zeiss LSM800 Airyscan laser scanning confocal microscope (Carl Zeiss MicroImaging ...
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bioRxiv - Neuroscience 2020Quote: ... The midbrain and eyestalk neural tissue was dissected free from the exoskeleton and its enveloping sheath in ice-cold chromating solution comprising 1 part 25% glutaraldehyde (Electron Microscopy Sciences; Cat# 16220; Hatfield, PA), 5 parts 2.5% potassium dichromate (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies used were 6 nm anti-mouse immunogold particles (Electron Microscopy Sciences). Negative staining was carried out as previously described [17] ...
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bioRxiv - Neuroscience 2024Quote: ... and then with 0.8 nm gold particle goat anti-rabbit antibody (EMS 25100) overnight ...
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bioRxiv - Biophysics 2021Quote: The nanodisc incorporated protein was individually applied to freshly glow-discharged Quantifoil R1.2/1.3 Cu300 mesh grids (Electron Microscopy Sciences). Grids were blotted for 4 s under 100% humidity and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... 3.5 μl of protein solution were applied onto the glow discharged Quantifoil Cu R1.2/1.3 200 grids (Electron Microscopy Sciences). The grids were blotted for 3s and plunge frozen into liquid ethane using a Vitrobot (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: 3 μl of proteins (~0.05 mg/ml) diluted with PBS buffer were deposited onto carbon-coated grids (EMS CF200-Cu) glow-discharged for 15 seconds at 25 mA in air ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 1 mg/ml (final for protein) and applied to freshly glow-discharged C-flat 300 mesh copper grids (CF-1.2/1.3, Electron Microscopy Sciences) at 4℃ ...
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bioRxiv - Biochemistry 2023Quote: ... the same proteins were applied to holey grids with a 2 nm carbon film (Quantifoil Cu 2/2 200 mesh from EMS). Samples were frozen after a total of 145 min on the grids without carbon film and 150 min on the grids with carbon film ...
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bioRxiv - Genetics 2023Quote: ... Nucleic acid and protein staining were performed using 4% solutions of Uranyl Acetate (UA) (Electron Microscopy Sciences cat. 22400-4) and Phosphotungstic acid hydrate (PTA ...
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bioRxiv - Biochemistry 2024Quote: ... 3.5 µL of protein sample were applied to freshly glow discharged Quantifoil R1.2/1.3 Cu 200 mesh grids (EMS, Q225-CR1.3) and were frozen by plunging into liquid ethane using an FEI Vitrobot Mark IV (100% humidity ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated overnight at 4°C in a 1:100 dilution of Goat anti-rabbit IgG (H+L) Ultrasmall EM grade 0.8 nm colloidal gold (Aurion, Electron Microscopy Sciences, Fort Washington, PA, cat #25121-0.6ml). Following washes in IEM buffer ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated with anti-rabbit secondary antibody conjugated to gold particles (Electron Microscopy Sciences) in the blocking solution for 1 h ...
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bioRxiv - Cell Biology 2021Quote: Exocyst holocomplex was mixed with liposomes composed of 95 mol% POPC and 5 mol% PI(4,5)P2 and protein A coated 5 nm gold fiducials (Electron Microscopy Sciences) for 3 minutes and to a final protein concentration of 1 μM ...
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bioRxiv - Cell Biology 2022Quote: ... proteins in gels were transferred to nitrocellulose membranes and subsequently immobilized by 0.1% glutaraldehyde (15 min) (Electron Microscopy Sciences; Hatfield, PA). After blocking in Tris-Buffered Saline containing 0.1% Tween 20 (TBS-T ...
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bioRxiv - Biochemistry 2020Quote: ... Cryo-EM grids were prepared by applying 3.5 μl of protein to the glow-discharged Quantifoil R1.2/1.3 200-copper mesh grids from Electron Microscopy Science (Q2100-CR1.3). The grids were blotted for 3 s ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was applied to glow discharged holey carbon grids which was then back blotted before adding 3 µL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μL protein solution at 0.01 mg/mL was added to a glow-discharged carbon-coated copper grid (Electron Microscopy Science). Grids were stained in 2% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... Both sets of grids were then back blotted before adding 3 μL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Bioengineering 2024Quote: ... A small drop (2.5 mL) of the protein solution was applied to the carbon side of a glow-discharged grid (EMS, CF300-CU) and left on the grid for 1 min ...
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bioRxiv - Bioengineering 2024Quote: ... 5 µl of protein complex was deposited on a holey copper grid (QUANTIFOIL R1.2/1.3, 200 mesh, EMS cat# Q250-CR1.3) or a carbon film copper grid (QUANTIFOIL R 1.2/1.3 ...
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bioRxiv - Plant Biology 2024Quote: ... The primary antibodies were visualized with anti-rabbit IgG (10nm; SKU.25109, Electron Microscopy Sciences) and anti-chicken (15 nm ...
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bioRxiv - Biochemistry 2021Quote: ... A volume of 4 µl protein samples was applied to glow discharged Quantifoil R1.2/1.3 grids (Electron Microscopy Sciences, Hatfield, PA, USA) using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
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bioRxiv - Neuroscience 2021Quote: ... and immediately placed on dry ice for protein or RNA extraction or fixed in fresh 4% paraformaldehyde (PFA, #1570-S, Electron Microscopy Science) for histological study ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
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bioRxiv - Biochemistry 2023Quote: ... protein was diluted to a concentration of 25 nM and applied to CF400-copper ultra-thin support carbon grids (Electron Microscopy Sciences) that were glow discharged with a PELCO easiGlow (Ted Pella ...
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bioRxiv - Biochemistry 2023Quote: ... the protein samples were diluted to 0.05 mg/mL in their respective dialysis buffers and 3.5 μL of the protein samples were applied to freshly glow-discharged formvar-reinforced carbon grids (EMS: FCF-200-AU-EC), washed with water ...
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bioRxiv - Biochemistry 2024Quote: ... 10 μl sample aliquots (protein with liposomes or liposomes alone) were adsorbed to glow-discharged 400-mesh carbon-coated copper grids (Electron Microscopy Sciences, EMS, USA) and stained with 0.75% uranyl formate and 1% uranyl acetate ...
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bioRxiv - Neuroscience 2024Quote: ... A secondary antibody (goat anti-mouse IgG conjugated to 15 nm colloidal gold; Electron Microscopy Sciences) diluted 1:20 in 0.1% BSA in PBS was added for 1.5 h ...
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bioRxiv - Biochemistry 2024Quote: ... 10 μl sample aliquots (protein with liposomes or liposomes alone) were adsorbed to glow-discharged 400-mesh carbon-coated copper grids (Electron Microscopy Sciences, EMS, USA) and stained with 0.75% uranyl formate and 1% uranyl acetate ...
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bioRxiv - Microbiology 2022Quote: ... Secondary antibodies used were goat-anti-Rabbit IgG conjugated to 10nm gold particles (Electron Microscopy Sciences, Cat. # 25109). We also labeled the samples with secondary gold-conjugated antibody alone to test for the non-specific labeling and found virtually no labeling in each case (data not shown) ...
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bioRxiv - Molecular Biology 2023Quote: ... segments were rinsed thoroughly with PBS and incubated overnight with the secondary antibody Ultra small (0.8 nm) Gold conjugated (EMS) in blocking buffer at RT ...
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bioRxiv - Cell Biology 2024Quote: ... segments were rinsed thoroughly with PBS and incubated overnight with the secondary antibody Ultra small (0.8 nm) Gold conjugated (EMS) in blocking buffer at RT ...
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bioRxiv - Neuroscience 2023Quote: ... 3.5 μL of CopiaGag capsid protein sample was applied to a 300-mesh copper grid with continuous 2 nm carbon film (Electron Microscopy Sciences, QUANTIFOIL® C2-C15nCu30-50) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
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bioRxiv - Cell Biology 2021Quote: Secondary antibodies: donkey anti-rabbit IgG (H&L) conjugated with 25-nm gold particles (Electron Microscopy Science Aurion #25708, PA, USA), donkey anti-mouse IgG (H&L ...
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bioRxiv - Developmental Biology 2024Quote: Larvae were dissected to optimally visualize the body wall muscles in ice-cold HL3.1 buffer and fixed with 4% paraformaldehyde in HL3.1 for 20 minutes for all antibodies except GluRIIA for which samples were fixed in Bouin’s fixative (Electron Microscopy Sciences, 15990) for 5 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with a secondary antibody conjugated with electron-dense colloidal gold nanoparticles of 10 nm size (Aurion, Electron Microscopy Sciences). GFP (1:500 ...
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bioRxiv - Microbiology 2023Quote: ... The grids were washed twice with blocking buffer before labeling with secondary antibodies (goat anti-rat or goat anti-rabbit, 10nm, Electron Microscopy Sciences), rinsed three times in 1X PBS ...