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Citations for Electron Microscopy Sciences :
751 - 800 of 895 citations for Primary Human Brain Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Cells were grown on glass coverslips in 12-well plates and fixed with 4% formaldehyde (Electron Microscopy Sciences) in PBS ...
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bioRxiv - Immunology 2023Quote: ... Cell post-fixation was as follows: 1 hour at 4°C with 1% osmium tetroxide (Electron Microscopy Sciences) and 0,8% potassium ferricyanide in PBS ...
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bioRxiv - Immunology 2024Quote: ... Cells were fixed by adding a warm double strength fixative solution (5% glutaraldehyde (GA, Electron Microscopy Sciences, #16310) and 4% paraformaldehyde (PFA ...
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bioRxiv - Cell Biology 2024Quote: Cells were plated on glass bottom 96-well plates and fixed using 4% paraformaldehyde (15710, Electron Microscopy Sciences) in DPBS (14190250 ...
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bioRxiv - Cell Biology 2019Quote: Cells were washed briefly in PBS and fixed for 10 minutes in PBS containing 4% paraformaldehyde (Electron Microscopy Sciences) at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... cells expressing GCD1-γ-HA and Gcn3-α-HA were fixed via treatment with 3.7% paraformaldehyde (EMS, Hatfield, USA) for at least 30 minutes followed by 45 minutes incubation in spheroplasting buffer (100 mM phosphate buffer pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then plated as attached OPCs on 0.01% Poly-L-Lysine coated plates (Electron Microscopy Sciences, 19320-B) in the same media ...
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bioRxiv - Cell Biology 2019Quote: HEK293 Flp-ln T-REx cells were grown on poly-L-lysine-coated #1.5 glass coverslips (Electron Microscopy Sciences). Cells were treated with 1 μg/mL doxycycline for 24 h to induce expression of BirA* or BirA*-CNAα ...
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bioRxiv - Cell Biology 2019Quote: ... HeLa cells were grown on 22mm glass coverslips (#1.5) pre-treated with 0.01% poly-L-lysine (Electron Microscopy Sciences) and then coated with 10μg/ml laminin (Cultrex ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed twice with CyFACS and fixed overnight at 4°C with 2% PFA (Electron Microscopy Sciences) in metal contaminant-free PBS (Rockland) ...
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bioRxiv - Synthetic Biology 2021Quote: ... media was aspirated from the MEA wells and cells were fixed with 2.5% glutaraldehyde (Electron Microscopy Sciences, PA, USA) and 2% paraformaldehyde (Electron Microscopy Sciences ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were grown on glass coverslips with or without collagen and fixed with 4% paraformaldehyde/PBS (Electron Microscopy Sciences). For the invadopodia assay on collagen-coated coverslips ...
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bioRxiv - Cell Biology 2021Quote: ... The attached cells were fixed with methanol and stained using a DIFF stain kit (Electron Microscopy Sciences, Hatfield, PA). Images of adherent cells were captured using the Keyence BZ-X710 microscope ...
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bioRxiv - Microbiology 2021Quote: ... Percentage of EGFP expressing cells was calculated after 24 hours by fixing samples with 4% paraformaldehyde (Electron Microscopy Sciences) and analyzing with imaging flow cytometry ...
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bioRxiv - Microbiology 2021Quote: ... 4 ml of cell suspension was spun down at 10000 g and pellets were fixed in glutaraldehyde solution 2.5% (EMS) and in osmium tetroxide 1% (EMS ...
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bioRxiv - Cell Biology 2022Quote: Cells seeded on 0.17 thickness 12 mm coverslips (Carl Roth) were fixed in 4% paraformaldehyde (PFA, Electron Microscopy Sciences)/PBS for 30 min ...
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bioRxiv - Cell Biology 2019Quote: Cells in plates or coverslips were washed three times with PBS and fixed in 4% paraformaldehyde (Electron Microscopy Sciences) in PBS for 20 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... and lysed by vortexing for 10 minutes with cell disruptor beads (0.1-mm diameter; Electron Microscopy Sciences, Hatfield, PA). Lysate was transferred to an 8ml glass vial with an additional 1ml cold methanol rinse of the lysis tube ...
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bioRxiv - Microbiology 2022Quote: ... Then the cells were washed three times with PBS and either immediately fixed with 4% formaldehyde (Electron Microscopy Sciences) in PBS for microscopy ...
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bioRxiv - Immunology 2022Quote: Cells on 96-well glass bottom plates were washed in PBS0 and fixed in 4% formaldehyde (Electron Microscopy Sciences) for 5 min at RT ...
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bioRxiv - Genetics 2019Quote: ... with 15 µl subsequently loaded on a CELLOMETER™ Disposable Cell Counting Chamber (Electron Microscopy Sciences, Hatfield, PA, USA) and analyzed under a Zeiss Axioskop 2 (Zeiss ...
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bioRxiv - Molecular Biology 2020Quote: ... infected and uninfected cells were fixed by rapidly transferring Aclar slabs into Karnovsky’s fixative (2% Glutaraldehyde (EMS cat.#16000) and 4% PFA (EMS cat.#15700 ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were then resuspended in 1 mL of 4% PFA (Electron Microscopy Sciences, cat. #15714S, diluted in PBS) and incubated at 4°C for 15 minutes with rocking ...
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bioRxiv - Biophysics 2021Quote: ... Cells were fixed for 20 min with a mixture of prewarmed (37°C) 0.25% glutaraldehyde (Electron Microscopy Sciences 16220) and 4% formaldehyde (Electron Microscopy Sciences 15714 ...
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bioRxiv - Immunology 2020Quote: ... The cells were washed and then fixed in FACS buffer supplemented with 2% PFA (15710-S, Electron Microscopy Sciences) for 15 minutes at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... Fixation of cells was performed with 3.7% (w/v) pre-warmed paraformaldehyde (PFA, EM Grade, Electron Microscopy Sciences, US) freshly diluted in PBS for 20 minutes at room temperature unless stated otherwise ...
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bioRxiv - Physiology 2023Quote: ... A few scraped cells were replated at a lower density on poly-D-lysine coated coverslips (Electron Microscopy Sciences) for imaging following fixation 24 hours after replating.
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bioRxiv - Microbiology 2023Quote: ... Bacteria cells were placed in 100 mesh copper grids containing the support carbon-coated Formvar film (Electron Microscopy Sciences) and air dried ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed and fixed for 20 min with 4% paraformaldehyde (PFA) in PBS (157-4, Electron Microscopy Sciences). For immunofluorescent detection and click chemistry ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were washed once with PBS+ and then fixed in PBS containing 4% paraformaldehyde (Electron Microscopy Sciences, 15713) for 30 minutes at room temperature ...
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bioRxiv - Genomics 2023Quote: ... Cells were harvested by centrifugation and fixed at room temperature while nutating in 4% paraformaldehyde (Electron Microscopy Sciences #15710). Cells were washed with PBS with 2mM EDTA and permeabilized at 45°C with 2% w/v sodium lauroyl sarcosine (Bioworld #41930024-3 ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed 3X with CyFACS buffer and fixed overnight at 4°C with 2% PFA (Electron Microscopy Sciences) in metal contaminant-free PBS ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were washed with cold flow cytometry buffer and fixed with 2% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA). The samples were stored at 4°C wrapped in foil up to 1 week prior to flow cytometric analysis ...
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bioRxiv - Cell Biology 2022Quote: Chemical fixation: Cells were fixed with 2,5 per cent glutaraldehyde (GA)/2,0 per cent paraformaldehyde (PFA) (Electron Microscopy Sciences) in 0.1M NaCac buffer (pH 7.4 ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... cells were rinsed with PBS containing calcium and magnesium and fixed using 2% paraformaldehyde (PFA) (Electron Microscopy Sciences # 15710) in PBS for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... Cells on transwell filters in well plates were fixed with 4% paraformaldehyde (#15710, Electron Microscopy Sciences, Hatfield, PA, USA) in PBS for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells plated on glass bottom dishes and platelets immobilized on poly-L-lysine (Electron Microscopy Sciences, Hatfield, PA)-coated on glass bottom dishes were fixed with 4.0 % paraformaldehyde (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... until the majority of cell nuclei were visible and mounted onto a formvar-supported slot grid (Electron Microscopy Sciences). Sections were imaged using a Hitachi H7500 TEM at 75 kV and 2048×2048 pixel ...
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bioRxiv - Neuroscience 2023Quote: Cells were pre-fixed with 2% paraformaldehyde/2.5% glutaraldehyde and post-fixed in 2% Osmium tetroxide (OsO4; EMS, USA). After block staining with 1.5% potassium ferrocyanide (sigma ...
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bioRxiv - Cell Biology 2024Quote: 1 × 106 cells in HBS were fixed using 4% paraformaldehyde (PFA) (cat no. 15710; Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... cells were first washed with PBS for 5 minutes and then fixed with 4% paraformaldehyde (Electron Microscopy Sciences, PA, USA) in PBS containing 0.1% Triton X-100 (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... cells were rinsed with pre-warmed HBSS and fixed using pre-warmed 2.5 % (w/v) glutaraldehyde (Electron Microscopy Sciences, 16220), 0.1 % tannic acid (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... trypsinized cells in FACS buffer (PBS with 1% BSA) were fixed using 4% paraformaldehyde (cat no. 15710; Electron Microscopy Sciences) for 20 min at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Cell were then fixed for 10 minutes at room temperature in PBSM + 4% paraformaldehyde (Electron Microscopy Sciences 50-980-492). Following fixation ...
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bioRxiv - Microbiology 2021Quote: ... Three μL of cell culture were applied to Cflat-2/1 200 mesh copper or gold grids (Electron Microscopy Sciences) glow discharged for 30 seconds at 15 mA ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended cells were passed through a 40 µm filter and fixed with 3mL of 1.33% formaldehyde (Electron Microscopy Sciences #15710) for 10 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... the media is removed from the wells and the cells are fixated via a 4% paraformaldehyde (PFA, Electron Microscopy Sciences) solution incubation for 10 minutes ...
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bioRxiv - Bioengineering 2019Quote: Smooth muscle cells (A10 and MOVAS) were fixed in methanol-free 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, cat. no. 15710) for 10 mins diluted in PBS++ (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: Cells were seeded at 0,2 x 106 cells/mL in 24-well plates containing 12 mm round coverslips (#1.5 mm thickness, Electron Microscopy Science) with 1 mL per well and stimulated as described above ...
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bioRxiv - Immunology 2021Quote: ... The cells were further washed twice with 1x permeabilization buffer (eBioscience) before fixation in 1.6% PFA solution (Electron Microscopy Sciences) containing 125 nM Iridium (Fluidigm ...