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Citations for Electron Microscopy Sciences :
751 - 788 of 788 citations for Dickkopf related protein 1 DKK 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: Purified receptor was diluted to ∼5 ug mL−1 and applied to freshly glow-discharged EM carbon coated copper grid (Electron Microscopy Sciences, CF400-Cu,) for 30 sec ...
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bioRxiv - Biochemistry 2022Quote: ... A 2.3-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 seconds using a PELCO easiGlow™ Glow Discharge Cleaning System ...
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bioRxiv - Microbiology 2021Quote: ... A 2.4-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 seconds using a PELCO easiGlow™ Glow Discharge Cleaning System ...
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bioRxiv - Neuroscience 2022Quote: ... EGFP expression in OB was detected using a dual fluorescent protein flashlight (Electron microscopy sciences, Hatfield, PA, USA) prior to surgery ...
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bioRxiv - Cell Biology 2022Quote: ... 2-3 μl of protein was applied to glow-discharged carbon-coated copper grids (EMS; CF-400-Cu) and incubated for 45 s at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 3μL of protein was deposited on holey carbon copper grids (QUANTIFOIL R 1.2/1.3-2nm, 300 mesh, EMS) which had been glow-discharged for 20s at 15 mA using PELCO easiGlow (TedPella Inc) ...
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bioRxiv - Biophysics 2023Quote: ... 5 μL of protein sample was applied to freshly-ionized carbon-coated copper mesh grids (Electron Microscopy Science). After 15 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Four microliters of protein was loaded onto a glow-discharged CF200-CU carbon grid (Electron Microscopy Sciences, U.K.). After 5 min of incubation ...
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bioRxiv - Immunology 2023Quote: ... A 2.4-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 s using a PELCO easiGlow Glow Discharge Cleaning System ...
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bioRxiv - Cell Biology 2021Quote: Samples were fixed in mixture of 4% paraformaldehyde (TAAB Laboratories Equipment Ltd, United Kingdom, cat. F/P001) and 1% glutaraldehyde (Electron Microscopy Science, USA, cat. 16310) in 0.1 M phosphate buffer (pH 7.4 ...
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bioRxiv - Genetics 2019Quote: ... 4 µL of sample at a final concentration of 0.01 mg/mL for high salt conditions or 0.1 mg/mL for low salt conditions were absorbed for 30 seconds on a glow-discharged carbon coated grid (Electron Microscopy Sciences, ref CF300-CU). The grids were then negatively stained for 1 minute using 2% uranyl formate ...
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bioRxiv - Molecular Biology 2022Quote: 3 μl of protein solution were applied to glow-discharged carbon coated copper grid (300 mesh, Electron Microscopy Science) and let adsorb for 30 s ...
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bioRxiv - Biochemistry 2023Quote: ... 4 μL of the protein solution was applied to a glow-discharged Formvar-carbon grid (EMS FCF200-Cu-UB) for 1 min followed by blotting ...
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bioRxiv - Biophysics 2023Quote: A volume of 3.5 μl of purified channel protein was applied onto glow-discharged copper holey carbon grids R1.2/1.3 (Q350CR1.3, Electron Microscopy Sciences), which was cleaned for 1 minute in an H2/O2 plasma using a Gatan Solarus 950 (Gatan) ...
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bioRxiv - Plant Biology 2020Quote: ... After 1 hour the leaf discs were washed three times with sterile water and then mounted in Fluoromount-G ™ (Electron microscopy Sciences, Hatfield, PA, USA). Leaf discs were imaged with a Zeiss LSM800 Airyscan laser scanning confocal microscope (Carl Zeiss MicroImaging ...
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bioRxiv - Neuroscience 2020Quote: ... The midbrain and eyestalk neural tissue was dissected free from the exoskeleton and its enveloping sheath in ice-cold chromating solution comprising 1 part 25% glutaraldehyde (Electron Microscopy Sciences; Cat# 16220; Hatfield, PA), 5 parts 2.5% potassium dichromate (Sigma Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... and midbrain and eyestalk neural tissue was dissected free from the exoskeleton and enveloping sheath in an ice-cold fixative solution containing 1 part 25% glutaraldehyde (Electron Microscopy Sciences; Cat# 16220; Hatfield, PA) to 5 parts 2.5% potassium dichromate (Sigma Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... Aliquots of 3.5 μl of the overnight actin/dynamin reaction mixture with 10 nm gold fiducials was loaded onto glow-discharged gold C-flat grids (Electron Microscopy Sciences, CF-1.2/1.-4Au-50) for 20 sec ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated overnight at 4°C in a 1:100 dilution of Goat anti-rabbit IgG (H+L) Ultrasmall EM grade 0.8 nm colloidal gold (Aurion, Electron Microscopy Sciences, Fort Washington, PA, cat #25121-0.6ml). Following washes in IEM buffer ...
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bioRxiv - Biophysics 2021Quote: The nanodisc incorporated protein was individually applied to freshly glow-discharged Quantifoil R1.2/1.3 Cu300 mesh grids (Electron Microscopy Sciences). Grids were blotted for 4 s under 100% humidity and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... 3.5 μl of protein solution were applied onto the glow discharged Quantifoil Cu R1.2/1.3 200 grids (Electron Microscopy Sciences). The grids were blotted for 3s and plunge frozen into liquid ethane using a Vitrobot (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: 3 μl of proteins (~0.05 mg/ml) diluted with PBS buffer were deposited onto carbon-coated grids (EMS CF200-Cu) glow-discharged for 15 seconds at 25 mA in air ...
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bioRxiv - Biochemistry 2023Quote: ... the same proteins were applied to holey grids with a 2 nm carbon film (Quantifoil Cu 2/2 200 mesh from EMS). Samples were frozen after a total of 145 min on the grids without carbon film and 150 min on the grids with carbon film ...
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bioRxiv - Genetics 2023Quote: ... Nucleic acid and protein staining were performed using 4% solutions of Uranyl Acetate (UA) (Electron Microscopy Sciences cat. 22400-4) and Phosphotungstic acid hydrate (PTA ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 1 mg/ml (final for protein) and applied to freshly glow-discharged C-flat 300 mesh copper grids (CF-1.2/1.3, Electron Microscopy Sciences) at 4℃ ...
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bioRxiv - Cell Biology 2021Quote: Exocyst holocomplex was mixed with liposomes composed of 95 mol% POPC and 5 mol% PI(4,5)P2 and protein A coated 5 nm gold fiducials (Electron Microscopy Sciences) for 3 minutes and to a final protein concentration of 1 μM ...
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bioRxiv - Cell Biology 2022Quote: ... proteins in gels were transferred to nitrocellulose membranes and subsequently immobilized by 0.1% glutaraldehyde (15 min) (Electron Microscopy Sciences; Hatfield, PA). After blocking in Tris-Buffered Saline containing 0.1% Tween 20 (TBS-T ...
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bioRxiv - Biochemistry 2020Quote: ... Cryo-EM grids were prepared by applying 3.5 μl of protein to the glow-discharged Quantifoil R1.2/1.3 200-copper mesh grids from Electron Microscopy Science (Q2100-CR1.3). The grids were blotted for 3 s ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was applied to glow discharged holey carbon grids which was then back blotted before adding 3 µL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μL protein solution at 0.01 mg/mL was added to a glow-discharged carbon-coated copper grid (Electron Microscopy Science). Grids were stained in 2% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... Both sets of grids were then back blotted before adding 3 μL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Biochemistry 2021Quote: ... A volume of 4 µl protein samples was applied to glow discharged Quantifoil R1.2/1.3 grids (Electron Microscopy Sciences, Hatfield, PA, USA) using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
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bioRxiv - Neuroscience 2021Quote: ... and immediately placed on dry ice for protein or RNA extraction or fixed in fresh 4% paraformaldehyde (PFA, #1570-S, Electron Microscopy Science) for histological study ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
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bioRxiv - Biochemistry 2023Quote: ... protein was diluted to a concentration of 25 nM and applied to CF400-copper ultra-thin support carbon grids (Electron Microscopy Sciences) that were glow discharged with a PELCO easiGlow (Ted Pella ...
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bioRxiv - Biochemistry 2023Quote: ... the protein samples were diluted to 0.05 mg/mL in their respective dialysis buffers and 3.5 μL of the protein samples were applied to freshly glow-discharged formvar-reinforced carbon grids (EMS: FCF-200-AU-EC), washed with water ...
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bioRxiv - Neuroscience 2023Quote: ... 3.5 μL of CopiaGag capsid protein sample was applied to a 300-mesh copper grid with continuous 2 nm carbon film (Electron Microscopy Sciences, QUANTIFOIL® C2-C15nCu30-50) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...