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Citations for Electron Microscopy Sciences :
751 - 800 of 1176 citations for 2 Amino 6 chloro 9 3 5 di O p toluoyl beta D 2 deoxyribofurnanosyl purine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Slices were incubated with Aurion blocking solution for one hour and washed 2 × 15 mins with incubation solution (Electron microscopy sciences, Cat # 25558). Then the slices were incubated with goat anti-BirA in incubation solution (1:50 ...
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bioRxiv - Immunology 2024Quote: ... 3 µL complex was mixed with 1 µL 20 µM lauryl maltose neopentyl glycol (LMNG) and immediately applied to glow discharged C-Flat™-2/1-3Cu-T50 (Electron Microscopy Sciences) holey grids at 4 °C and 100 % humidity inside a Vitrobot Mark IV ...
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bioRxiv - Developmental Biology 2024Quote: About ∼50 million H1-GATA6+/+ cells at day 2 or day 4 of cardiac differentiation were fixed with 1% formaldehyde (Electron Microscopy Sciences 15710) for 8 min at RT with gentle agitation ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were rinsed in 0.15 M cacodylate buffer 3x for 20 minutes each before incubation in a 2% OsO4 in 0.15 M cacodylate buffer for 1.5 hours in the dark (19152, Electron Microscopy Sciences, Hatfield, PA, USA). Post-OsO4 incubation ...
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bioRxiv - Neuroscience 2024Quote: ... at 1 : 1 for 30 min and 2 : 1 for 30 min and twice with pure Durcupan (Electron Microscopy Sciences, Hatfield, PA, USA) for 30 min each ...
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bioRxiv - Molecular Biology 2023Quote: ... The output list of CandiSNP was screened for nonsynonymous amino acid changes and the G/C to A/T transitions that were likely to be caused by EMS, with a special focus on the chromosome with the highest SNP density with an allele frequency of 1 ...
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bioRxiv - Plant Biology 2023Quote: ... The output list of CandiSNP was screened for non-synonymous amino acid changes and the G/C to A/T transitions that were likely to be caused by EMS, with a particular focus on the chromosome with the highest density of SNPs with an allele frequency of >0.75.
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bioRxiv - Microbiology 2020Quote: Double CsCl gradient-purified HAdV particles were adhered to Collodion and 2% (v / v) amyl acetate film-covered grids (300 mesh Formvar/carbon-supported copper support films, Electron Microscopy Sciences, Hatfield, USA). Viral particles were negatively stained with 2% (v / v ...
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bioRxiv - Cell Biology 2021Quote: ... were applied to carbon-coated and glow discharged (2 minutes at 7x10-1 mbar) 300-mesh copper EM grids (Electron Microscopy Sciences; CF300-CU) and incubated for four minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed with FACS buffer and once with PBS before fixing with 200 mL 2% paraformaldehyde (PFA; Electron Microscopy Sciences, Cat# 15710) in PBS overnight or longer ...
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bioRxiv - Microbiology 2022Quote: ... thick resin sections (250 nm) were cut and placed on a slot (1 × 2 mm) EM copper grids covered with a formvar film (Electron Microscopy Sciences, FF2010-Cu). Grids were decorated with fiducial marker and stained with Hoechst to visualize nuclear regions ...
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bioRxiv - Biochemistry 2023Quote: ... 2.5mM Mg(OAc)2 wash buffer to an optical density of 2 A260/mL (corresponding to 40 nM of 80S) and deposited on copper grids (Electron Microscopy Sciences, CF400-CU). Grids were glow-discharged at 15 mA for 30s 4 uL of sample were incubated on the glow-discharged grids for 1min ...
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bioRxiv - Neuroscience 2022Quote: Postnatal mouse were transcardially perfused with 15ml (P14) or 25ml (>P21) of 2% PFA freshly prepared from 32% PFA solution (Electron Microscopy Sciences, 50-980-495). Perfused brains were dissected out ...
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bioRxiv - Neuroscience 2021Quote: ... cultures were washed in imaging buffer to remove non-internalised HRP and fixed with a solution of 2 % glutaraldehyde (Electron Microscopy Sciences, Hatfield, USA, 16019) in phosphate buffered saline ...
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bioRxiv - Neuroscience 2023Quote: ... cultures were washed in imaging buffer to remove non-internalised HRP and fixed with a solution of 2 % glutaraldehyde (Electron Microscopy Sciences, Hatfield, USA, 16019) and 2% PFA in 0.1 M in phosphate buffer (PB) ...
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bioRxiv - Neuroscience 2023Quote: ... isoflurane overdose was used to deeply anesthetize mice and cold PBS was transcardially perfused for 2 minutes followed by 7 minutes of perfusion with 4%PFA freshly made from 32% (Electron Microscopy Sciences Cat #15714-S) at a rate of 7 mL/min ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were fixed for at least 2 hours (at room temperature) to overnight (at 4° C) in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) in artificial sea water ...
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bioRxiv - Neuroscience 2022Quote: ... tissue was dehydrated through an ethanol series (70% ethanol, 90% ethanol, 2 x 100% ethanol; 8 minutes each) and mounted in Entellan (Electron Microscopy Science, Hatfield, PA, USA).
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bioRxiv - Neuroscience 2024Quote: mice were perfused 7 dpI and 10 dpl with 25 mL of 2% PBS-paraformaldehyde freshly prepared from 32% PFA solution (Electron Microscopy Sciences, 50-980-495). Perfused brains were dissected out ...
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bioRxiv - Microbiology 2024Quote: ... This concentrate was diluted 1:2 with molecular grade water and 50 µL was fixed with 25% glutaraldehyde (stored at 4°C, Electron Microscopy Sciences, Hatfield, PA, USA) to a final concentration of 0.25% ...
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bioRxiv - Neuroscience 2024Quote: ... 70 nm ultrathin sections were taken and post-stained with 2% aqueous uranyl acetate and lead citrate (22400, 22405, 17800, 17900, 21140, Electron Microscopy Sciences, Hatfield, PA, USA). Samples were imaged on a TEM (Jeol JEM-1400 Plus ...
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bioRxiv - Biochemistry 2024Quote: ... 4 µl of sample were added to freshly glow-discharged 300 mesh 2/1 µm C-flat copper grids (Electron Microscopy Sciences, Hatfield, Pennsylvania, USA), blotted for 10 s at blot force 4 at 100% relative humidity and 4°C before being plunge frozen in a liquid mix of 37% (v/v ...
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The hedgehog signaling pathway is expressed in the adult mouse hypothalamus and modulated by fastingbioRxiv - Neuroscience 2021Quote: ... C57Bl/6 mice using 1.0 mm Militex Biopsy Punch (Electron Microscopy Sciences). There was a minimum of 6 animals per treatment group ...
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bioRxiv - Neuroscience 2020Quote: ... After 3 rinses in Cacodylate buffer (EMS, 11650), serial cuts of 80 µm thick were made with vibratome ...
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bioRxiv - Cell Biology 2022Quote: ... 3 times in propylene oxide (Electron Microscopy Sciences), 3 times in 50% resin (24g Embed 812 ...
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FUS controls muscle differentiation and structure through LLPS mediated recruitment of MEF2 and ETV5bioRxiv - Neuroscience 2024Quote: ... After 3 rinses in Cacodylate buffer (EMS, 11650), muscles were post fixed in 0.5% osmium and 0.8% potassium ferrocyanide in Cacodylate buffer 1h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... and 3% lead citrate solution (Electron Microscopy Sciences) for 5 min each ...
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bioRxiv - Cell Biology 2021Quote: Cells were cultured on cell-tight C-2 cell disks (Sumitomo Bakelite, Tokyo, Japan; #MS-0113K) and fixed for 2 h on ice in 2.5% glutaraldehyde (Electron Microscopy Science, Hatfield, PA; #111-30-8) in 0.1 M phosphate buffer ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... cells were transferred to poly-D lysine-coated coverslips (Electron Microscopy Sciences) where ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... cells were transferred to poly-D lysine-coated coverslips (Electron Microscopy Sciences) and allowed to adhere ...
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bioRxiv - Neuroscience 2023Quote: ... 3.5 μL of CopiaGag capsid protein sample was applied to a 300-mesh copper grid with continuous 2 nm carbon film (Electron Microscopy Sciences, QUANTIFOIL® C2-C15nCu30-50) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies used were 6 nm anti-mouse immunogold particles (Electron Microscopy Sciences). Negative staining was carried out as previously described [17] ...
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bioRxiv - Developmental Biology 2023Quote: The abt4-1 (ttg2-6) mutant was isolated from an ethyl methanesulfonate (EMS)-mutagenesis mutant population in gl2-3 mutant background (Salk_039825 ...
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bioRxiv - Cell Biology 2024Quote: Cell lysates were first mixed with 6 nm gold fiducials (Electron Microscopy Sciences) to facilitate tilt series alignment during data processing ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then fixed with 3% PFA (EMS, 15710) in PBS for 10 minutes at RT ...
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bioRxiv - Cell Biology 2024Quote: ... and post-stained with 3% Reynold’s lead citrate (EMS) for 1 minute ...
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bioRxiv - Bioengineering 2024Quote: ... incubated with 3% glutaraldehyde (#16539-60; Electron Microscopy Sciences) for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 3% lead citrate (Electron Microscopy Sciences 22410). Grids were imaged at 2900x on a FEI Tecnai T12 transmission electron microscope with a 16 Mpx camera (Advanced Microscopy Techniques Corp).
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bioRxiv - Cell Biology 2022Quote: ... fine-tipped forceps (Dumont #5, EMS). Holding the coverslip vertically ...
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bioRxiv - Synthetic Biology 2022Quote: ... The sample attached to the grid was stained by immediately tapping the grid on 2% w/w uranyl acetate (Electron Microscopy Sciences, Hatfield, PA, USA, Cat. No. 22400) solution droplets twice ...
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bioRxiv - Molecular Biology 2022Quote: Cells were plated on poly-D-lysine coated glass coverslips (Electron Microscopy Sciences) and fixed with 4% paraformaldehyde for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... H were fixed with warm 3% PFA (Electron Microscopy Sciences) in PBS containing 0.4% w/v Sucrose for 15 minutes at room temperature and quenched with 0.25% ammonium chloride (SIGMA ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed with 3% paraformaldehyde (Electron Microscopy Sciences 15714) with 0.02%-0.1% glutaraldehyde (GA ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 minute and then 3% Lead Citrate (EMS 22410) for 6 minutes ...
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bioRxiv - Microbiology 2021Quote: ... fixed in 3% (vol/vol) glutaraldehyde (Electron Microscopy Sciences [EMS]) for approx ...
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bioRxiv - Microbiology 2021Quote: ... fixed in 3% (vol/vol) glutaraldehyde (Electron Microscopy Sciences [EMS]) for approx ...
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bioRxiv - Cell Biology 2020Quote: ... 3 hours before fixation with 2.5 % glutaraldehyde (Electron microscopy Sciences) in 0.1M Na+- cacodylate for 1 hour or overnight at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... for 3 hours and subsequently treated with 1% glutaraldehyde (EMS) for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... Fixation was conducted using 3% formaldehyde solution (Electron Microscopy Sciences) in PHEM buffer (1.8% PIPES ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were fixed using 3% paraformaldehyde (Electron Microscopy Sciences) in 1X PBS for 10 minutes ...