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Citations for Electron Microscopy Sciences :
651 - 700 of 3026 citations for trans 2 2 4 Methoxyphenyl 2 oxoethyl cyclohexane 1 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Tissues were submerged in 1.5% uranyl acetate in absolute methanol at -90 °C for 2 days and infiltrated with HM20 Lowicryl resin (Electron Microscopy Sciences) over 2 days at -45 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were washed with acetone and infiltrated with increasing concentrations (25, 50, 75 and 100%; 2 hours each) of EPON resin (Electron Microscopy Sciences). 100% EPON solution was exchanged two times in 12-hour steps ...
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bioRxiv - Biophysics 2022Quote: ... They were then kept at room temperature for ∼2 h before being applied to carbon-coated 200-hex copper grids (EMS, Hatfield PA). Freshly ionized grids were floated on a 10 ul drop of sample for 1 minute ...
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bioRxiv - Biophysics 2022Quote: U-2 OS cells expressing pRFPs-mito were seeded into 96-well optical polymer base microplates and fixed with 2% paraformaldehyde (Electron Microscopy Sciences) and 2.5% glutaraldehyde (Electron Microscopy Sciences ...
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bioRxiv - Physiology 2020Quote: 1-2 tracheal rings were removed post-mortem and embedded in Peel-A-Way embedding molds containing Tissue-Tek OCT (Electron Microscopy Sciences). Molds were placed in a container filled with dry ice until frozen ...
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bioRxiv - Genetics 2021Quote: ... 3 μl of the diluted 3C library was deposited onto a plasma-clean Quantifoil R2/2 holey carbon grid (Electron Microscopy Sciences), blotted with a filter paper for 2 seconds ...
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bioRxiv - Immunology 2021Quote: ... EM grids were prepared by staining 1:20 and 1:100 dilutions (in PBS plus 250 mM NaCl) of lyophilised and resuspended sample with 2 % uranyl formate solution on carbon-coated CF400-CU-UL grids (Electron Microscopy Sciences) as described earlier ...
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bioRxiv - Neuroscience 2020Quote: ... the slice was placed in a fixative consisting of 2% formaldehyde (diluted from 8% formaldehyde, EM grade, Electron Microscopy Sciences 157-8) and 2 % glutaraldehyde (diluted from 25% glutaraldehyde ...
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bioRxiv - Microbiology 2021Quote: ... Denatured RNA was refolded in the presence of 2 μM of LNA ASO by incubating the mixture in 340 mM sodium cacodylate buffer (Electron Microscopy Sciences) and 5 mM MgCl2+ ...
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bioRxiv - Biophysics 2022Quote: ... Holey gold films with ~2 μm holes were nanofabricated as described previously (Marr et al., 2014) on 300 mesh Maxtaform copper-rhodium grids (Electron Microscopy Sciences). Specimens with 10 mM ATP were prepared by first applying 0.4 μL of 50 mM ATP in buffer (70 mM Tris-HCl pH 7.4 ...
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bioRxiv - Neuroscience 2020Quote: ... Rbp4-Cre;Ai14;Snap25+/+ and Rbp4-Cre;Ai14;Snap25fl/fl brains at P18 were perfused with 0.1 M phosphate buffer containing 2% PFA (Electron Microscopy Sciences, 15714) and 2.5% glutaraldehyde (Electron Microscopy Sciences ...
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bioRxiv - Cell Biology 2021Quote: Mice were sacrificed by cervical dislocation and sciatic nerve were exposed and fixed by adding fixative solution (2% PFA (15710, Electron Microscopy Sciences), 2.5% Glutaraldehyde (16220 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were gradually returned to 175 mM PB in decreasing fructose concentrations over 16 hrs and then post-fixed in 2% GA in 150 mM sodium cacodylate buffer (CB, Electron Microscopy Sciences) for 2 hrs.
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bioRxiv - Pathology 2021Quote: Liver tissue samples were fixed in 0.1 M cacodylate-buffered Karnovsky fixative containing 2.5% glutaraldehyde and 2% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) overnight at room temperature with a subsequent post-fixation in 1% osmium tetroxide (Electron Microscopy Sciences) ...
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bioRxiv - Biochemistry 2020Quote: ... The grids were washed by dipping in two separate drops of water followed by two drops of 2% uranyl acetate (Electron Microscopy Sciences). Grids were examined at the Northwestern Electron Probe Instrumentation Center (EPIC ...
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bioRxiv - Plant Biology 2021Quote: ... LPI-1 from growth chamber plants or young leaves of tissue culture plants were processed for primary fixation at 25°C in 2 % glutaraldehyde (Electron Microscopy Sciences, EMS) prepared with Sorensen’s Phosphate buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then fixed using Karnovsky Fixative Reagent Grade (3% glutaraldehyde and 2% formaldehyde in 0.1 M phosphate buffer, pH 7.4) (Electron Microscopy Sciences, 15732-10) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... They were carefully placed on a fine layer of resin between 2 sheets of ACLAR® embedding films (EMS, cat# 50425-25) for polymerization at 55 °C for 72 h ...
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bioRxiv - Neuroscience 2022Quote: ... was perfused with 10 mL of 1x PBS followed by 30 mL of fixative 2% PFA/0.075% GA in PBS (32% PFA Alfa Aesar, 3% GA Electron Microscopy Science). Group 2 (indirect PFA ...
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bioRxiv - Bioengineering 2022Quote: ... the cholangiocyte organoids were fixed with TEM fixative containing 2% paraformaldehyde/2.5 glutaraldehyde in 100mM cacodylate buffer (Electron Microscopy Sciences, Hartfield, PA) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... followed by ice-cold EM fixative (1.25% glutaraldehyde [Electron Microscopy Sciences, 16220], 2% paraformaldehyde [Electron Microscopy Sciences, 19210], 0.1M sodium cacodylate buffer). Brains were removed and stored for 8 days in EM fixative at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... About 4 µl of aerated and hypoxia samples were pipetted on glow-discharged Quantifoil Au extra thick EM-grids (R2/2, 200 mesh, Electron Microscopy Sciences). The extra fluid was blotted (blot force of 7 s and blot time 5 sec ...
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bioRxiv - Microbiology 2023Quote: 3.5 µL of Vipp1 at a final concentration of 60 µM was incubated for 90 seconds on a plasma cleaned holey R2/2 Quantifoil copper grid (Electron Microscopy Science) before plunge freezing in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Biophysics 2023Quote: ... Cells were washed with pre-warmed (37°C) PBS and permeabilized for 2 minutes using 0.3% glutaraldehyde (GA) + 0.25% Triton X-100 (EMS, 16220 and ThermoFisher, 28314) in pre-warmed (37 °C ...
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bioRxiv - Plant Biology 2024Quote: Leaf tissue was collected from the interveinal region of the youngest fully expanded leaves and fixed in 2% glutaraldehyde (Electron Microscopy Sciences, EMS) and 2.5 % paraformaldehyde (Ted Pella Inc) ...
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bioRxiv - Biochemistry 2023Quote: ... Resuspended culture was mixed with 10 nm gold beads as fiducial markers and then applied onto glow-discharged copper R2/2 200 mesh Quantifoil holey carbon grids (Electron Microscopy Sciences) in Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... One end of the tube was clamped with a hemostat and the tube was filled with room temperature 2% paraformaldehyde (PFA) (Electron Microscopy Sciences) with a syringe and metal cannula ...
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bioRxiv - Cell Biology 2022Quote: 10 μl of concentrated Pseudotyped SARS-CoV-2 S lentiviral particles were incubated for 30 min on carbon coated grids (EMS #CF200-CU). Excess solution was removed by back blotting ...
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bioRxiv - Cell Biology 2024Quote: Media were formulated as previously described [2] to create disease progression from Normal Fasting (NF) to Early Metabolic Syndrome (EMS or MASLD) and Late Metabolic Syndrome (LMS or late stage MASLD) ...
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bioRxiv - Microbiology 2023Quote: ... and 1% tannic acid (Electron Microscopy Sciences, Hatfield, PA). After 2 hours ...
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bioRxiv - Biophysics 2024Quote: ... a 20-μL drop of 1% phosphotungstic acid (1%; Electron Microscopy Sciences SKU 19502-1), and three 20-μL drops of deionized water were sequentially placed on a piece of parafilm ...
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bioRxiv - Biophysics 2022Quote: ... The grids were then stained with 50 μL aqueous uranyl acetate solution (2% v/v) (Electron Microscopy Sciences, Philadelphia, USA. Product No: 22400) for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... Brains of 3–7day old male flies were dissected in 1X PBS medium (BPP3920, Fisher Sci) and fixed in 2% paraformaldehyde (PFA, Electron Microscopy Sciences #15710) in PBT for 60 min at room temperature (RT) ...
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bioRxiv - Plant Biology 2021Quote: ... LPI-1 from growth chamber plants or young leaves of tissue culture plants were processed for primary fixation at 25°C in 2 % glutaraldehyde (Electron Microscopy Sciences, EMS) prepared with Sorensen’s Phosphate buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Fibril assemblies were next analyzed via transmission electron microscopy at an applied concentration of 0.4 mg per mL, with fibrils absorbed to glow-discharged 300 mesh (Ladd Research Industries, Inc.) and stained with 2% uranyl acetate (Electron Microscopy Sciences, Inc.) in water ...
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bioRxiv - Bioengineering 2022Quote: ... The tethered EVs were fixed in a 2 % glutaraldehyde (MilliporeSigma, Burlington, MA) and 0.1 M sodium cacodylate solution (Electron Microscopy Sciences, Hatfield, PA) for 3 hr ...
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bioRxiv - Plant Biology 2024Quote: Leaf tissue was collected from the interveinal region of the youngest fully expanded leaves and fixed in 2% glutaraldehyde (Electron Microscopy Sciences, EMS) and 2.5 % paraformaldehyde (Ted Pella Inc) ...
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bioRxiv - Bioengineering 2022Quote: ... VLP samples were fixed with 2% (v/v) glutaraldehyde in water solution on 400 mesh Formvar-coated copper grids (EMS Cat#FF400-Cu). After fixation ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were incubated with Aurion blocking solution for one hour and washed 2 × 15 mins with incubation solution (Electron microscopy sciences, Cat # 25558). Then the slices were incubated with goat anti-BirA in incubation solution (1:50 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were rinsed in 0.15 M cacodylate buffer 3x for 20 minutes each before incubation in a 2% OsO4 in 0.15 M cacodylate buffer for 1.5 hours in the dark (19152, Electron Microscopy Sciences, Hatfield, PA, USA). Post-OsO4 incubation ...
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bioRxiv - Cell Biology 2024Quote: Primary neurons or astrocytes cultured on glass coverslips at the same plating density as was used for live cell imaging experiments were fixed for 10 minutes at 37°C/5% CO2 in a solution containing 2% paraformaldehyde (PFA; Electron Microscopy Sciences 15713) and 0.05% glutaraldehyde in PHEM buffer (EMS ...
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bioRxiv - Developmental Biology 2022Quote: ... Staining was performed with 1% aqueous tannic acid (EMS #21700) followed by 1% aqueous uranyl acetate (Analar #10288 ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were stained successively with 1% tannic acid (EMS) and 1% uranyl acetate (Serva ...
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Photoreceptor disc incisures form as an adaptive mechanism ensuring the completion of disc enclosurebioRxiv - Cell Biology 2023Quote: ... tadpoles were treated with 1% tannic acid (Electron Microscopy Sciences) and 1% uranyl acetate (Electron Microscopy Sciences) ...
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bioRxiv - Microbiology 2020Quote: Double CsCl gradient-purified HAdV particles were adhered to Collodion and 2% (v / v) amyl acetate film-covered grids (300 mesh Formvar/carbon-supported copper support films, Electron Microscopy Sciences, Hatfield, USA). Viral particles were negatively stained with 2% (v / v ...
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bioRxiv - Genetics 2022Quote: ... Dissected gonads were fixed in 2 mL 3% paraformaldehyde (PFA) (10 mL 16% PFA, EM Grade, Electron Microscopy Sciences, Hatfield, PA Catalog No 15710) phosphate-buffered solution for 10 minutes at room temperature and then post-fixed with 2 mL 100% methanol (Gold-label from Fisher ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed with FACS buffer and once with PBS before fixing with 200 mL 2% paraformaldehyde (PFA; Electron Microscopy Sciences, Cat# 15710) in PBS overnight or longer ...
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bioRxiv - Systems Biology 2023Quote: ... Slides were washed twice for 5 min in wash buffer and fixed for 5 min in diluted glutaraldehyde solution 2% (Electron Microscopy Sciences, Hatfield, PA) in PBS-low barium (Ionpath ...
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bioRxiv - Biochemistry 2023Quote: ... 2.5mM Mg(OAc)2 wash buffer to an optical density of 2 A260/mL (corresponding to 40 nM of 80S) and deposited on copper grids (Electron Microscopy Sciences, CF400-CU). Grids were glow-discharged at 15 mA for 30s 4 uL of sample were incubated on the glow-discharged grids for 1min ...
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bioRxiv - Immunology 2020Quote: ... Grids were negatively stained using 1% phosphotungstic acid (Electron Microscopy Sciences) for 1 min and the excess removed using a filter paper ...