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Citations for Electron Microscopy Sciences :
651 - 700 of 821 citations for N acetylglucosamine 1 phosphodiester alpha N acetylglucosaminidase NAGPA Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Formvar carbon film on 200-mesh copper grids (FCF200-Cu) and uranyl formate (16984-59-1) from Electron Microscopy Sciences were used for sample preparation for negative-stain transmission electron microscopy (TEM).
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bioRxiv - Physiology 2024Quote: Isolated TALs and DCTs were embedded into 1% agarose and fixed in 3% formaldehyde/glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) for 24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... Subsequently, sections were incubated in DAPI (1:1000, 10 mins) and mounted with Fluoromount G (Electron Microscopy Sciences, Hatfield, PA).
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bioRxiv - Molecular Biology 2024Quote: ... and cover-slipped with ThermoFisher Gold Seal Cover Glass 24 x 60mm No.1 thickness (Electron Microscopy Sciences, 63770-01). Sections were imaged using a Leica CTR6000/B microscope to quantify sulfatide accumulation in different brain areas.
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bioRxiv - Biophysics 2024Quote: ... 2.5 µl of sample was applied to 300 mesh Quantifoil R 2/1 copper grids (Electron Microscopy Sciences, Hatfield PA) that had been previously glow discharged (15 mA ...
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bioRxiv - Biochemistry 2024Quote: The specimen was vitrified using 3 μL of freshly purified BasC-Nb71 complex in 0.17% DM applied to glow-discharged Quantifoil R 0.6/1 Cu 300 mesh grids (Electron Microscopy Sciences), at a concentration of 2.2 mg/ml ...
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bioRxiv - Genetics 2023Quote: AG437 pezo-1(R2405P) early L4 hermaphrodites were washed three times in M9 and soaked in 48 mM Ethyl methane sulfonate (EMS) solution for four hours at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... pelleted and washed with PBS before being resuspended in 1 mL of 4% PFA (Electron Microscopy Sciences 30525-89-4) + 0.0075% glutaraldehyde (Sigma G6257 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were then incubated in 0.1 M sodium cacodylate buffer supplied with 1% OsO4 and 0.8% potassium ferricyanide (Electron Microscopy Sciences), for 2 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL of the infected and control cultures were fixed in paraformaldehyde (Electron Microscopy Sciences, ref. 15714, 1% final conc.) and glutaraldehyde (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... 3 µl of 1 mg/ml 70S ribosome RafH complex was applied on a glow discharged 300 CF300-Cu grids (EMS), the excess sample was blotted ...
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bioRxiv - Biochemistry 2023Quote: ... A sample of 3.0 μM of FH2 and 10.6 μM of actin monomers was applied to holey grids without a carbon film (Quantifoil Au 2/1 300 mesh from EMS). After diluting 4-fold ...
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bioRxiv - Cell Biology 2023Quote: ... samples were washed in water and stained for 1 h at room temperature in 2% uranyl acetate (Electron Microscopy Sciences), and then washed again in water and dehydrated in a graded series of ethanol ...
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bioRxiv - Bioengineering 2023Quote: Purified DNA origami structures (1–10 nM) were adsorbed onto freshly glow discharged copper grids (Electron Microscopy Sciences, Hatfield, PA) and incubated for 4 minutes ...
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bioRxiv - Genetics 2023Quote: The two epididymides of mature males were recovered in 1 mL of 0.1 M sodium cacodylate buffer (pH 7.4, Electron Microscopy Sciences) and the sperm were allowed to swim for 15 min at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... transcardial perfusion was performed using 1× phosphate-buffered saline (PBS) and 4% paraformaldehyde (PFA; Electron Microscopy Sciences, the United States). The mouse liver and kidney were dissected and postfixed in 4% PFA for 6 hours at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... an overlay of rat tail collagen type 1 solution (2.5 mg/mL) in NF medium (or EMS for disease conditions) was deposited on top of the hepatocytes inside the borders of the oval-well ...
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bioRxiv - Pathology 2024Quote: ... the sections were heated to 650C for 1-2 hours to deparaffinize and treated with Histoclear II (Electron Microscopy Sciences) twice for 15 minutes each ...
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bioRxiv - Neuroscience 2024Quote: ... 500ul 1% neutral red dye in PBS was injected intraperitoneally 2 hours prior to cardiac perfusion with ice cold fixation buffer (2.5% glutaraldehyde, 1% paraformaldehyde, in 0.2 M sodium cacodylate buffer (Electron Microscopy Sciences). Lesion tissues were then dissected out and cut into 2mmx2mm sections ...
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bioRxiv - Immunology 2024Quote: Neutrophils migrating under the agarose for 1 h in 8 well chamber dishes were fixed using 4% paraformaldehyde (PFA, EMS #) in PHEM buffer for 20 min at 37 °C followed by agarose removal from the wells ...
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bioRxiv - Microbiology 2024Quote: ... 1 mL of culture was sampled (Fig. S1) and chemically fixed by adding paraformaldehyde (PFA; Electron Microscopy Science; EM grade) to a final concentration of 2% ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were washed in water and stained for 1 hour at room temperature in 2% uranyl acetate (Electron Microscopy Sciences), washed again in water and dehydrated in a graded series of ethanol ...
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bioRxiv - Physiology 2024Quote: ... pH 7.4 for 2-weeks followed by incubation in a PBS solution containing 1% osmium tetroxide (Electron Microscopy Sciences, 19170) and 2.5% potassium dichromate (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: Enucleated adult eyes were fixed for 1 hour at 4°C in 4% paraformaldehyde (PFA, Electron Microscopy Science, Hatfield, PA) prepared in phosphate-buffered saline (1X PBS ...
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bioRxiv - Microbiology 2024Quote: ... sections were cut with an ultra 45° Diatome™ diamond knife on a Leica Ultracut UCT microtome and collected on formvar support film slots 2×1 mm cu grids (EMS). Sections were counterstained with 2% ethanolic uranyl acetate ...
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bioRxiv - Biophysics 2024Quote: ... 3 µL of each sample (∼1 mg/mL) was applied to a freshly glow discharged (15mA, 1 min) holey carbon copper grid (apo-37C: Cflat (EMS) R1.2/1.3 ...
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bioRxiv - Biophysics 2024Quote: ... 2.5% glycerol) was applied to glow discharged gold grids (Au-flat, 0.6 µm/1 µm, 300 mesh, Electron Microscopy Sciences). The sample was blotted with the minimum blot time that can be set (0 s) ...
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bioRxiv - Biochemistry 2024Quote: ... AFM samples were prepared by adsorbing 10 μl of 5 times diluted sample onto mica (Muscovite mica, V 1 quality, EMS) for 45 minutes ...
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bioRxiv - Biophysics 2024Quote: ... This cell suspension (3 µl) was applied to Cflat-2/1 200 mesh copper or gold grids (Electron Microscopy Sciences) that were glow discharged for 30 seconds at 15 mA ...
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bioRxiv - Bioengineering 2024Quote: ... mice were perfused transcardially with heparinized saline (10 units mL−1 of heparin) and 4% paraformaldehyde (PFA) that was freshly prepared from 32% PFA aqueous solution (Cat# 15 714, EMS), using a peristaltic pump at a rate of 7 mL min−1 ...
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bioRxiv - Cell Biology 2024Quote: ... Sapphire discs with attached frozen hTERT-RPE-1 cells and type-A planchettes containing worms were transferred to cryo-vials filled with anhydrous acetone containing 1% (w/v) osmium tetroxide (EMS) and 0.1% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... and placed on a slot (1 × 2 mm) EM copper grids covered with a formvar film (Electron Microscopy Sciences, FF2010-Cu). Grids were placed (section face-down ...
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bioRxiv - Biophysics 2021Quote: The purified bison NHA2ΔN protein was individually applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences). Grids were blotted for 3.0 s or 3.5 s under 100% humidity and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... The slices were then transferred to capped glass vials and postfixed with 1% osmium tetroxide diluted in PBS from a 4% aqueous solution (EMS 19170) for 30 minutes on a rotator ...
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bioRxiv - Microbiology 2020Quote: ... Diluted S protein was mixed with a 1.5-fold molar excess of ACE2 and the mixture was incubated on ice for 1 minute before 4.8 uL of the protein mixture was deposited on a CF400-Cu grid (Electron Microscopy Sciences) before being stained with methylamine tungstate (Nanoprobes) ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM EDTA) and 5 μL of the mixture was applied to carbon-coated 300 mesh Cu grids (Electron Microscopy Sciences) after glow discharging for 1 min at −15 mA in an EasiGlo glow discharger (Ted Pella) ...
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bioRxiv - Cell Biology 2021Quote: ... 4 mM NaHCO3, 1 mM NaH2PO4, 5 mM trehalose, 10 mM sucrose, 5 mM HEPES, pH 7.5, prepared by Electron Microscopy Sciences) and then bathed in whole organ ex vivo culture medium with 10 µg/ml isradipine (Selleck Chemical LLC ...
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bioRxiv - Biochemistry 2020Quote: ... 4μL of the resulting solution was deposited on glow-discharged Quantifoil grids (R2/1, Cu 200-mesh grid, Electron Microscopy Sciences). The solution on the grid was then blotted using a Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of sample (0.5 mg/ml) were applied to freshly glow-discharged (see above) C-flat 2/1-3C-T grids (Electron Microscopy Sciences) and plunged into liquid ethane after 2 s blot time with relative blot force set to 21 ...
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bioRxiv - Synthetic Biology 2021Quote: ... 10 µL of resuspended cells were pipetted onto agarose pads in 13 x 1 mm silicone isolator wells (Electron Microscopy Sciences) and covered with a No ...
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bioRxiv - Plant Biology 2021Quote: Marchantia thalli were cut in small pieces (1×2 mm) and fixed immediately in buffered phosphate 0,1 M paraformaldehyde 4% (Electron Microscopy Sciences), glutaraldehyde 2,5% (TAAB Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were resin infiltrated in ethyl alcohol and Spurr’s resin epoxy mixtures using an automated EMS Lynx 1 EM tissue processor (Electron Microscopy Sciences). Processed samples were infiltrated with two changes of fresh Spurr’s resin for 24 hours and the samples were then oriented in the resin ...
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bioRxiv - Neuroscience 2021Quote: ... or to fit a stack of custom laser-cut quartz glass coverslips (three coverslips with #1 thickness each (Electron Microscopy Sciences), cut to a ‘D’-shape with maximum dimensions of 5.5 mm medial-lateral and 7.7 mm anterior-posterior ...
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bioRxiv - Systems Biology 2020Quote: ... rinsed with 0.1M sodium cacodylate buffer pH 7.4 (5X1min) on ice and then were posted fixed with 1% osmium tetroxide (19150, Electron Microscopy Sciences) containing 0.8% potassium ferrocyanide ...
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bioRxiv - Systems Biology 2020Quote: ... the La2-DAB solution was removed and cells were rinsed with 0.1M sodium cacodylate buffer pH 7.4 (5X1min) on ice and then were posted fixed with 1% osmium tetroxide (19150, Electron Microscopy Sciences) containing 0.8% potassium ferrocyanide ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μL of the EV sample was adsorbed for 1 min to a carbon-coated grid (# CF400-CU, Electron Microscopy Sciences) that had been made hydrophilic by a 20-sec exposure to a glow discharge (25mA) ...
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bioRxiv - Neuroscience 2021Quote: ... or to fit a stack of custom laser-cut quartz glass coverslips (three coverslips with #1 thickness each (Electron Microscopy Sciences), cut to a ‘D’-shape with maximum dimensions of 5.5 mm medial-lateral and 7.7 mm anterior-posterior ...
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bioRxiv - Cell Biology 2021Quote: ... cells were gently washed with 30 μL of PBS and fixed with 1% (v/v) paraformaldehyde (Electron Microscopy Sciences 15710-S) diluted in PBS at room temperature for 20 min ...
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bioRxiv - Cancer Biology 2022Quote: ... at 37 °C for 20 minutes prior to fixation in periodate–lysine–paraformaldehyde (PLP) buffer (1% paraformaldehyde (PFA; Electron Microscopy Science), 0.01M sodium periodate ...
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bioRxiv - Biophysics 2022Quote: ... 3.5 μL of purified TTP at 1.5 mg mL-1 was applied to a 400-mesh copper lacey carbon-coated grid (Electron Microscopy Sciences) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...