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Citations for Electron Microscopy Sciences :
6501 - 6550 of 7095 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... fixed with 4% paraformaldehyde (PFA, EMS), and then permeabilized and blocked with blocking solution (0.5% TritonX-100 ...
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bioRxiv - Cell Biology 2019Quote: ... or 1% Triton X-100 and 3.2% paraformaldehyde (EM grade; EMS) for 10 min ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were fixed for 1.5 hours at room temperature in 2.5% glutaraldehyde and paraformaldehyde (EMS) in 0.1 M sodium cacodylate buffer ...
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bioRxiv - Cell Biology 2019Quote: ... infiltrated in Lowicryl HM20 resin (Electron Microscopy Science) for 10 h at −45°C ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were washed and mounted using Fluoromount-G™ (Electron Microscopy Sciences) before imaging.
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bioRxiv - Cell Biology 2019Quote: ... Immunolabelled sections were mounted in Fluorogel (Electron Microscopy Sciences, PA; Cat 17985-10) and visualized using a Zeiss ApoTome microscope ...
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bioRxiv - Cell Biology 2019Quote: ... and samples were embedded in Durcupan ACM resin (Electron Microscopy Science). Polymerization was performed by incubating samples at 60°C overnight ...
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bioRxiv - Cell Biology 2019Quote: ... cells were fixed at 10-15 min post-damage for 45 min with 4% paraformaldehyde (PFA; Cat No – RT-15710, Electron Microscopy Sciences) in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... for 10 minutes at RT and washed twice with ice-cold PBS before mounting onto glass slides with 5 µl Fluoromount-G mounting medium (Electron Microscopy Sciences).
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bioRxiv - Cell Biology 2019Quote: ... followed by three times rinsing in pure acetone (EMS, Hatfield, USA) (20 min each time) ...
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bioRxiv - Cell Biology 2019Quote: ... washed three times with ice-cold PBS and fixed with 2% paraformaldehyde (Electron Microscopy Sciences) for 20 minutes at RT ...
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bioRxiv - Cell Biology 2019Quote: ... worm samples were rinsed in pure acetone (EMS, Hatfield, USA) for three times (20 min each time ...
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bioRxiv - Cell Biology 2019Quote: ... before adding 25 μL 16 % paraformaldehyde (Electron Microscopy Sciences 15710-S) (final PFA = 4 % ...
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bioRxiv - Cell Biology 2019Quote: ... organoids were washed 3 times with PBS and fixed for 30 min in 4% paraformaldehyde (Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.5% uranyl acetate (Agar Scientific, Stansted, UK) in acetone (EMS, Hatfield, USA) at -90 °C ...
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bioRxiv - Cell Biology 2019Quote: The following reagents/primary antibodies were used for immunofluorescence analyses: paraformaldehyde solution (10%) was purchased from Electron Microscopy Sciences (Hatfield, PA); mouse anti-TfR2 ...
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bioRxiv - Genetics 2019Quote: ... the kidneys were embedded in tissue freezing medium (Electron Microscopy Sciences) and cryo-cut at 10 μm (Leica CM3050S) ...
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bioRxiv - Microbiology 2019Quote: ... 5uL of sample was applied on a glow-discharged carbon-coated grids (300 mesh Cupper, EMS). After two washes with water and one wash with a drop of 0.75% uranyl formate ...
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bioRxiv - Microbiology 2019Quote: Equal numbers of bacteria were resuspended in sodium phosphate buffer (pH 6.8) and treated for 15 min at room temperature with 1% formaldehyde (Electron Microscopy Sciences Cat #15710) as previously described (29 ...
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bioRxiv - Microbiology 2019Quote: ... wild-type 81116 strain cell culture grown to OD600 = 5 was applied onto glow-discharged C-flat holey carbon films 2/2 200 mesh (EMS). A Leica EM GP (Leica ...
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bioRxiv - Microbiology 2019Quote: ... of purified protein at a concentration of 1 mg ml-1 was deposited onto glow-discharged C-flat holey carbon films 1.2/1.3 200 mesh (EMS). A Vitrobot Mark III (FEI ...
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bioRxiv - Microbiology 2019Quote: ... then fixed with 2% glutaraldehyde and 2% paraformaldehyde in PBS at pH 7.4 (Electron Microscopy Sciences) for 3 h at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... a drop of purified Pandoravirus massiliensis particles fixed with 2.5% glutaraldehyde in 0.1M cacodylate buffer was adsorbed onto formvar carbon films on 400 mesh nickel grids (FCF400-Ni, EMS). Grids were stained for 10 seconds with 1% molybdate solution in filtered water at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... cells were fixed in 2% paraformaldehyde (Cat# 15714S; Electron Microscopy Sciences, Hatfield, PA), and GFP positive cells quantified by flow cytometry (Intellicyt iQue Screener PLUS ...
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bioRxiv - Microbiology 2019Quote: ... plasmids encoding the DENV protein variants were transfected individually into human HEK-293T cells and allowed to express for 22 h before fixing cells in 4% paraformaldehyde (Electron Microscopy Sciences), and permeabilizing with 0.1% (w/v ...
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bioRxiv - Physiology 2019Quote: 1-2 tracheal rings were removed post-mortem and embedded in Peel-A-Way embedding molds containing Tissue-Tek OCT (Electron Microscopy Sciences). Molds were placed in a container filled with dry ice until frozen and stored at −80 °C long-term ...
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bioRxiv - Neuroscience 2019Quote: ... then moved into pure EPON resin (Electron Microscopy Sciences) (exchanged after 2 hrs ...
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bioRxiv - Physiology 2019Quote: ... Remaining adherent MΦs were fixed in 4% formaldehyde (Electron Microscopy Sciences) for 10 min followed by DAPI staining ...
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bioRxiv - Cell Biology 2019Quote: ... blocked for 30 minutes in filtered blocking solution containing: 0.5% gelatin (EMS), 0.5% BSA (MP biomedical ...
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bioRxiv - Cell Biology 2019Quote: ... resuspended in 1 ml fixative (40 mM KHPO4 pH 7.0 containing 1% EM grade glutaraldehyde and 0.2% EM grade formaldehyde from Electron Microscopy Sciences (EMS, Munich, Germany) and incubated for 3 min.
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bioRxiv - Cell Biology 2019Quote: ... resuspended in 1 ml fixative (40 mM KHPO4 pH 7.0 containing 1% EM grade glutaraldehyde and 0.2% EM grade formaldehyde from Electron Microscopy Sciences (EMS, Munich, Germany) and incubated for 3 min.
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bioRxiv - Cell Biology 2019Quote: ... resuspended in freshly prepared 2% KMnO4 (EMS) and incubated for 3 min ...
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bioRxiv - Cell Biology 2019Quote: ... 95% and 100% (v/v) ethanol (EMS). After a final wash in water-free ethanol ...
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bioRxiv - Cell Biology 2019Quote: ... cells were washed for 3 × 10 min with propylene oxide (EMS) and embedded in epon resin (Embed-812 ...
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bioRxiv - Cell Biology 2019Quote: ... and embedded in epon resin (Embed-812, EMS). Cells were infiltrated at room temperature with 25% resin in propylene oxide for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... pure resin at 30°C for 2 × 12 h and pure resin with 3% accelerator (BDMA, EMS) at 30°C for 12 h ...
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bioRxiv - Cell Biology 2019Quote: ... Sectioning was done on a Leica UC6 ultramicrotome and sections were collected on carbon coated 200 mesh copper grids (Electron Microscopy Sciences, Hatfield, PA). Grids with 90 nm thick sections were placed on a drop of water ...
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bioRxiv - Cell Biology 2019Quote: ... the staining agents 2% OsO4 (Osmiumtetraoxid, Electron Microscopy Sciences, Hatfield, PA, USA) and 3% potassium ferrocyanide (C6N6FeK4 ...
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bioRxiv - Cell Biology 2019Quote: HeLa cells grown on 25-mm photoetched coverslips (Electron Microscopy Sciences, Hatfield, PA) were transfected with 250 ng mApple-M1 Spastin and 100 ng mEmerald-SKL plasmids for ~16 hours in the presence of 15 μM oleic acid ...
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bioRxiv - Neuroscience 2019Quote: ... Embedding was performed in Durcupan ACM resin (Electron Microscopy Sciences, Hatfield, PA, USA). First ...
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bioRxiv - Neuroscience 2019Quote: ... NaNO2 (# S25560) and NaCl (# 7647-14-5) which is followed by perfusion with 40 mL of cold 4% paraformaldehyde (Electron Microscopy Sciences, #RT-15714) in 1X-phosphate buffered saline (PBS) ...
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bioRxiv - Neuroscience 2019Quote: ... pH-7.3 until they were completely saturated and later incubated in 1:1 mixture of 30% sucrose solution and optical cutting temperature (OCT) compound (Electron Microscopy Sciences, #62550-01) for 1-2 hours before embedding in OCT ...
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bioRxiv - Neuroscience 2019Quote: ... embedded in mounting media (Tissue-Tek® O.C.T. Compound, Electron Microscopy Sciences, Hatfield, PA, USA), and rapidly frozen on dry ice ...
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bioRxiv - Cell Biology 2019Quote: ... EM-grade paraformaldehyde (15714) and glutaraldehyde (16365) were purchased from Electron Microscopy Sciences (Hatfield, PA, USA). Fura-2/AM (F0888) ...
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bioRxiv - Microbiology 2019Quote: ... Gelatine blocks were prepared by spinning down this cell suspension at room temperature for 10 min at ∼ 5000 × g and an aliquot of the cell pellet was taken and made it to adhere at the bottom of the gelatine capsule (Electron Microscopy Sciences) and then capsule was filled with 100% LR White resin ...
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bioRxiv - Developmental Biology 2019Quote: ... gmIs18 or vab-8(e1017)gmIs18 worms were mutagenized for 4 hours by incubation in 0.05 M ethyl methane sulfonate (EMS). Worms were washed in M9 buffer (22mM KH2P04 ...
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bioRxiv - Cancer Biology 2019Quote: ... and surface antibodies were fixed in place by a 15-minute incubation with 1.5% paraformaldehyde (PFA; Electron Microscopy Sciences). Subsequently ...
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bioRxiv - Bioengineering 2019Quote: ... reduced with ferrocyanide and block-stained in 1% uranyl acetate (all reagents from Electron Microscopy Sciences). The material was then dehydrated through a graded ethanol series and embedded in Eponate12 (Ted Pella) ...
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bioRxiv - Immunology 2019Quote: ... Sections were counterstained in Van Gieson’s solution for 5 min and dehydrated (all reagents were purchased from Electron Microscopy Sciences, Hatfield, PA). All images were acquired with microscope Eclipse 80i.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and coverslipped with Eukitt mounting medium (Electron Microscopy Sciences, PA, USA).