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Citations for Electron Microscopy Sciences :
551 - 600 of 916 citations for Methyl 2 chloro 3 trifluoromethyl benzoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... cells were fixed in a final concentration of 2% paraformaldehyde (Cat# 15714S; Electron Microscopy Sciences) and % GFP positive cells were enumerated by flow cytometry (Intellicyt iQue Screener PLUS ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were post-fixed with a 2% paraformaldehyde and 2.5% glutaraldehyde solution (Electron Microscopy Science) in 0.15 M cacodylate buffer (pH 7.4 ...
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bioRxiv - Developmental Biology 2022Quote: ... the whole embryo was fixed in 2% EM-grade PFA in PBS (Electron Microscopy Sciences) for 1hr at room temperature or intestines were isolated by dissection under wholemount microscope (Leica microsystems ...
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bioRxiv - Bioengineering 2024Quote: ... glow-discharged with 25 mA for 2 min using an EMS 100X (Electron Microscopy Sciences) glow-discharged unit prior to sample application ...
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bioRxiv - Molecular Biology 2023Quote: ... mounted using DAPI (4ʹ,6- diamidino-2-phenylindol) Fluoromount G mounting medium (Electron Microscopy Science) and imaged with an Axio Observer microscope (Zeiss).
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bioRxiv - Cell Biology 2023Quote: ... 8% methanol and 2% water dissolved in glass-distilled acetone (Electron Microscopy Sciences, Hatfield, USA) at –90°C for 64 h and at –45°C for 24 h ...
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bioRxiv - Biophysics 2023Quote: ... and stained with 2% uranyl acetate and Reynolds lead citrate (Electron Microscopy Sciences, Hartfield, PA). Measurements were performed on the perpendicular cross-sections of 100 hyphae per culture condition using a JEOL JEM-1400 electron microscope (Peabody ...
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bioRxiv - Cell Biology 2023Quote: ... Post-fixation was performed using 2% (w/v) osmium tetroxide (Electron Microscopy Sciences, Hatfield, UAS) for 30 min in chilled cacodylate buffer ...
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bioRxiv - Biophysics 2024Quote: ... samples were thawed and inactivated for 2 hours at room temperature by adding PFA (EMS) to a final concentration of 4 % (v/v) ...
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bioRxiv - Immunology 2022Quote: ... The cells were washed and fixed in with 2% PFA (15710-S, Electron Microscopy Sciences) diluted in 0.2% FACS buffer for 15 minutes at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... for 2 days at room temperature (peritoneal cavity) or in 4% paraformaldehyde (Electron Microscopy Sciences) overnight at 4 °C (muscle tissue).
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bioRxiv - Cell Biology 2022Quote: ... 2 h in the dark in 0.5% (w/v) uranyl acetate (EMS, Hatfield, PA, USA) dissolved in 0.05 N maleic acid buffer (pH 5.2) ...
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bioRxiv - Plant Biology 2022Quote: ... picked up on a copper slot grid 2 x 1 mm (EMS, Hatfield, PA, US) coated with a polystyrene film (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were post-fixed with 2% osmium tetroxide (OsO4)/1.5% potassium ferrocyanide (EMS, Sigma, USA) for 1 hour on ice and washed several times ...
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bioRxiv - Cell Biology 2023Quote: Part of the sample collected as described above was fixed with 2% paraformaldehyde (EMS grade) and 0.5% glutaraldehyde (EMS grade ...
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bioRxiv - Cell Biology 2024Quote: ... specimens underwent post-fixation with 4% osmium tetroxide and 2% potassium ferrocyanide (Electron Microscopy Sciences) for 1 h ...
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bioRxiv - Bioengineering 2024Quote: ... 2 mg/mL sample was applied to the glow discharged gird (Electron Microscopy Sciences, 25486), followed by removing the extra sample amount using filter paper and quickly plunging into liquid ethane ...
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bioRxiv - Biophysics 2024Quote: ... 8% methanol and 2% water dissolved in glass-distilled acetone (Electron Microscopy Sciences, Hatfield, USA) at –90°C for 64 h and at –45°C for 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were dehydrated through a series of increasing concentration of ethanol (30%, 50%, 70%, 90%, 3 times 100% anhydrous) and embedded in EMBed 812 epoxy resin (EMS, Hatfield, PA). After resin polymerization ...
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bioRxiv - Bioengineering 2020Quote: ... were prepared by applying 3 µL of 10 mg/mL sample on pretreated lacey carbon 400 mesh TEM grids (Electron Microscopy Sciences). Following a 3 s blot ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
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bioRxiv - Cell Biology 2022Quote: ... falciparum 3D7 culture was pelleted by centrifugation (1500g, 3 min, RT) and resuspended in freshly prepared PBS containing 4% EM-grade para-formaldehyde (PFA, Electron Microscopy Sciences) and 0.1% EM-grade GA (Sigma ...
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bioRxiv - Biochemistry 2020Quote: Freshly prepared filament samples (3 μL) were applied to glow-discharged Lacey carbon film grids (LC200-Cu; Electron Microscopy Sciences, Hatfield PA). The grid was blotted for 2.0 s using Whatman #1 filter paper (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
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bioRxiv - Biophysics 2020Quote: ... the head was placed onto the scanner directly after cleaving the glued-on 3 mm Muscovite Mica disk (Electron Microscopy Sciences, Hatfield). The concentrated sample was diluted in imaging buffer to 60 µg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue was rinsed five times for 3 min in DDH2O and then dehydrated through graded alcohols into acetone and flat-embedded in Durcopan resin (Electron Microscopy Sciences) between mylar strips and placed in a 60°C oven for 48h.
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bioRxiv - Biophysics 2023Quote: ... and cryo-ET grids were prepared by applying 3 μL of sample to glow-discharged (30 s) Lacey carbon grids (Electron Microscopy Sciences). Grids were blotted on both sides for 6 s with 0 blotting force and vitrified immediately by plunge freezing into liquid nitrogen-cooled liquid ethane with an FEI Mark IV Vitrobot cryo-sample plunger ...
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bioRxiv - Genomics 2023Quote: ... the cells were washed five times with PBS-T and post-fixed with 3% paraformaldehyde and 0.1% glutaraldehyde (Electron Microscopy Sciences #16120) in 1X PBS for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were gradually dehydrated in an ethanol series (50%,75%,95%, 3×100%) and then embedded in EMbed-812 epoxy resin (Electron Microscopy Sciences), followed by polymerization for 48h at 60°C.Thin sections (70nm ...
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bioRxiv - Molecular Biology 2023Quote: Primed and naïve hESCs were prepared as described in the above section and fixed for 10 min in a 3% paraformaldehyde solution (Electron Microscopy Science). Cells were then permeabilized for 7 min with ice-cold PBS supplemented with 0.5% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... The buffer was removed and samples stained with post-fixative for 3 hours (Osmium tetroxide, Ted Pella; Tannic Acid, Electron Microscopy Sciences). After washing with distilled water ...
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bioRxiv - Microbiology 2023Quote: Negatively stained samples were made by standard methods: 3 µl of sample was placed on a glow-discharged grid with ultrathin carbon layered on lacey carbon (Electron Microscopy Sciences), washed with three drops of water and stained with 1% uranyl acetate ...
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bioRxiv - Immunology 2022Quote: ... and 3 µL of the complex/detergent mixture was immediately applied to QuantiFoil 300 mesh 1.2/1.3 grids (Electron Microscopy Sciences, Hatfield, PA) that had been freshly glow discharged with PELCO easiGLOW (Ted Pella ...
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bioRxiv - Immunology 2024Quote: ... At -60 °C samples were infiltrated with increasing concentrations (30, 60, and 100% for 1 h each) of Lowicryl (3:1 K11M/HM20 mixture; Electron Microscopy Sciences). After 5 h of 100% Lowicryl infiltration ...
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bioRxiv - Plant Biology 2024Quote: ... 4 µL of this sample at 2 µg Chl mL−1 was applied to a glow discharged (60 s, 25 mA) 400 mesh Cu grid with 5-6 nm formvar with 3-4 nm carbon (Electron Microscopy Sciences). The sample was negatively stained with 2% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... amylovora strain Ea110 were collected at 3 dpi and fixed in paraformaldehyde/ glutaraldehyde (2.5% of each compound in 0.1 M sodium cacodylate buffer) (Electron Microscopy Sciences, Hatfield, PA) at 25 C overnight ...
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bioRxiv - Biochemistry 2024Quote: 3 μg of purified NHE9*CC sample was applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences) and blotted for 3.0s with a 20s waiting time prior ...
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Protein identification using cryo-EM and artificial intelligence guides improved sample purificationbioRxiv - Biochemistry 2024Quote: ... 3 µL of the diluted sample was applied to a freshly glow discharged 400 carbon square mesh grid (Electron Microscopy Sciences, EMS) and allowed to adsorb onto the grid for 30 seconds ...
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Protein identification using cryo-EM and artificial intelligence guides improved sample purificationbioRxiv - Biochemistry 2024Quote: ... 3 µL of the diluted sample was applied to a freshly glow discharged 400 carbon square mesh grid (Electron Microscopy Sciences, EMS) and allowed to adsorb onto the grid for 30 seconds ...
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bioRxiv - Cancer Biology 2021Quote: ... and on a 30 μL droplet of 2% uranyl acetate solution (Electron Microscopy Sciences, Hatfield, PA) for 10 additional minutes at RT ...
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bioRxiv - Cell Biology 2020Quote: ... The coverslips were then moved immediately into unroofing buffer containing 2% glutaraldehyde (Electron Microscopy Sciences, 16019). With syringe ...
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bioRxiv - Biochemistry 2020Quote: ... cells at room temperature were fixed for 10 minutes with 2% formaldehyde (Electron Microscopy Sciences 15710), and permeabilized for one hour with 0.1% Triton X-100 (Biorad 1610407 ...
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bioRxiv - Neuroscience 2021Quote: ... sapphire disks were placed into 12-well plate containing 1 mL of 2% glutaraldehyde (EMS #16530) and 1 mM CaCl2 (Sigma-Aldrich # 63535 ...
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bioRxiv - Physiology 2020Quote: ... and stained with freshly prepared 2% aqueous solution of Osmium Tetroxide (Electron Microscopy Sciences, Cat#19150) in 0.1 M sodium cacodylate buffer (pH 7.4) ...
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bioRxiv - Cell Biology 2020Quote: ... and manually unroofed with 19-gauge needle and syringe using 2% paraformaldehyde (Electron Microscopy Sciences 15710) in intracellular buffer ...
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bioRxiv - Neuroscience 2022Quote: ... mice were sacrificed by transcardial perfusion with Karnovsky’s fixative: 2% glutaraldehyde (Electron Microscopy Sciences (EMS 16000) and 4% paraformaldehyde (EMS 15700 ...
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bioRxiv - Neuroscience 2022Quote: ... mice were sacrificed by transcardial perfusion with Karnovsky’s fixative: 2% glutaraldehyde (Electron Microscopy Sciences (EMS 16000) and 4% paraformaldehyde (EMS 15700 ...
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bioRxiv - Microbiology 2022Quote: ... cells were washed with 1XPBS and fixed for 15 min with 2% paraformaldehyde (Electron Microscopy Sciences) in 1XPBS ...
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bioRxiv - Cell Biology 2022Quote: ... The sample was subsequently imaged by FLIM or stored in 2% glutaraldehyde (Electron Microscopy Sciences, 16019) at 4°C until EM sample preparation.
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bioRxiv - Cell Biology 2022Quote: ... and manually unroofed with 19-gauge needle and syringe using 2% paraformaldehyde (Electron Microscopy Sciences, 15710) in the intracellular buffer ...