Labshake search
Citations for Electron Microscopy Sciences :
551 - 600 of 739 citations for Gins Complex Subunit 2 Psf2 Homolog Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... They were subsequently transcardially perfused with 15 ml of 0.1M sodium phosphate buffer pH 7.4 and then 60 ml of 2% paraformaldehyde (15710-S, Electron Microscopy Sciences) 1% glutaraldehyde (G7526 ...
-
bioRxiv - Microbiology 2023Quote: Genomic libraries were constructed and sequenced by MiSeq using paired-end sequencing (2 × 300 bp) for 17 strains generated by EMS mutagenesis and one strain ...
-
bioRxiv - Neuroscience 2023Quote: ... samples were washed 3–4 times 10 min each with fresh acetone chilled to −90°C and left at the same temperature for 6 hours in acetone containing 2% osmium (Science services (EMS)) and 0.2% uranyl acetate (Serva) ...
-
bioRxiv - Genetics 2023Quote: ... the supernatant was discarded and the pellet resuspended in primary fixating buffer (2% glutaraldehyde/0.1 M sodium cacodylate buffer, pH 7.4, Electron Microscopy Sciences) for 30 min at 4 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... the cells were fixed in 0.1 M cacodylate buffer (pH 7.4) with 2% glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) and 2% paraformaldehyde (Electron Microscopy Sciences ...
-
bioRxiv - Cell Biology 2022Quote: ... EVs were fixed by positioning the grid on top of a 20 µL drop of 2% paraformaldehyde (EMS, 157-4) for 10 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... the cells on nanostructures were washed with PBS and fixed in 0.1 M sodium cacodylate buffer (pH 7.2) supplemented with 2% glutaraldehyde (Electron Microscopy Sciences) and 4% paraformaldehyde (PFA ...
-
bioRxiv - Microbiology 2022Quote: ... 2 mL of the infected and control cultures were fixed in paraformaldehyde (Electron Microscopy Sciences, ref. 15714, 1% final conc.) and glutaraldehyde (Sigma ...
-
bioRxiv - Biochemistry 2023Quote: ... A sample of 3.0 μM of FH2 and 10.6 μM of actin monomers was applied to holey grids without a carbon film (Quantifoil Au 2/1 300 mesh from EMS). After diluting 4-fold ...
-
bioRxiv - Cell Biology 2023Quote: ... samples were washed in water and stained for 1 h at room temperature in 2% uranyl acetate (Electron Microscopy Sciences), and then washed again in water and dehydrated in a graded series of ethanol ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples were washed in water and stained for 1 hour at room temperature in 2% uranyl acetate (Electron Microscopy Sciences), washed again in water and dehydrated in a graded series of ethanol ...
-
bioRxiv - Biochemistry 2024Quote: Prom1 and Ttyh1 EVs were vitrified on 300-mesh gold Quantifoil R 1.2/1.3 + 2 nm Carbon grids (Electron Microscopy Sciences). Briefly ...
-
bioRxiv - Neuroscience 2024Quote: ... en bloc staining protocol (Kislinger et al., 2020a) (Kislinger et al., 2023) including post-fixation in 2% osmium tetroxide (EMS), 1.5% potassium ferrocyanide (Sigma ...
-
bioRxiv - Genetics 2024Quote: ... The coverslip was mounted onto a concave microscope slide to reduce media evaporation (2-Well Concavity Slide, Electron Microscopy Sciences). Images were acquired using an AX70 Olympus epifluorescence microscope across 11 z-planes with 0.5 µm of interplanar distance ...
-
bioRxiv - Microbiology 2024Quote: ... sections were cut with an ultra 45° Diatome™ diamond knife on a Leica Ultracut UCT microtome and collected on formvar support film slots 2×1 mm cu grids (EMS). Sections were counterstained with 2% ethanolic uranyl acetate ...
-
bioRxiv - Immunology 2024Quote: ... or 0.5 µl of 0.48 mM n-dodecyl-B-D-maltopyranoside (DDM) solutions immediately before deposition onto plasma-cleaned Quantifoil 2/1 grids (EMS). Grids were either blotted for 3 or 4 s before being plunged into liquid nitrogen-cooled liquid ethane using a Vitrobot mark IV (Thermo Fisher Scientific).
-
bioRxiv - Immunology 2024Quote: ... and cells were fixed and stained with solution containing final concentrations of 2% paraformaldehyde (PFA; Electron Microscopy Sciences, #15713-S) in DPBS ...
-
bioRxiv - Pathology 2024Quote: ... the sections were heated to 650C for 1-2 hours to deparaffinize and treated with Histoclear II (Electron Microscopy Sciences) twice for 15 minutes each ...
-
bioRxiv - Molecular Biology 2024Quote: ... a 2 μL sample was spotted onto a freshly glow-discharged carbon film 300 mesh copper grid (Electron Microscopy Sciences), incubated for 1 min ...
-
bioRxiv - Physiology 2024Quote: ... pH 7.4 for 2-weeks followed by incubation in a PBS solution containing 1% osmium tetroxide (Electron Microscopy Sciences, 19170) and 2.5% potassium dichromate (Sigma-Aldrich ...
-
bioRxiv - Physiology 2024Quote: Atrial and ventricular tissue samples were cut into pieces of about 1mm3 and fixed in a mixture of 2% glutaraldehyde (16316-10, EMS) and 2% formaldehyde (15700 ...
-
bioRxiv - Biochemistry 2024Quote: ... mesh 300 grids with 2 nm amorphous carbon layer on top were glow discharged for 30 s at 15 mA (EMS-100 Glow Discharge System ...
-
bioRxiv - Biophysics 2024Quote: ... This cell suspension (3 µl) was applied to Cflat-2/1 200 mesh copper or gold grids (Electron Microscopy Sciences) that were glow discharged for 30 seconds at 15 mA ...
-
bioRxiv - Developmental Biology 2024Quote: Larvae were fixed for 2 hours at room temperature or overnight at 4° C in 4% paraformaldehyde (EMS catalog # 15710) and 4% sucrose (MP Biomedicals catalog # 802536 ...
-
bioRxiv - Neuroscience 2024Quote: ... A secondary antibody (goat anti-mouse IgG conjugated to 15 nm colloidal gold; Electron Microscopy Sciences) diluted 1:20 in 0.1% BSA in PBS was added for 1.5 h ...
-
bioRxiv - Microbiology 2020Quote: ... and placed on a slot (1 × 2 mm) EM copper grids covered with a formvar film (Electron Microscopy Sciences, FF2010-Cu). Grids were placed (section face-down ...
-
bioRxiv - Molecular Biology 2021Quote: BF PolIE RNAi cells were grown with or without doxycycline for 24 hours then incubated with 2 mM of ethylmethanesulfonate (EMS; Sigma) or DMSO (vehicle ...
-
bioRxiv - Cancer Biology 2021Quote: ... slides were washed twice for 5 minutes in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
-
bioRxiv - Zoology 2021Quote: Sets of oocytes including some number of visibly infected individuals were fixed in 2% glutaraldehyde in 0.2 M sodium cacodylate buffer (SCB, Electron Microscopy Sciences) for one hour and post-fixed in 2% osmium tetroxide in SCB for one hour (Bozzola and Russell ...
-
bioRxiv - Microbiology 2021Quote: ... 0.1% uranyl acetate and 2% H2O followed by en bloc Osmium-Thiocarbohydrazide-Osmium staining75,76 and embedding in Durcupan ACM (Electron Microscopy Sciences). Serial block-face imaging was conducted in a Zeiss Sigma 3View system with variable pressure with 30 nm x 70 nm resolution at 5.5 kV ...
-
bioRxiv - Cell Biology 2021Quote: Osteoclasts plated on bone slices for 3 days were unroofed as described above and fixed for 10 min in a 0.2M sodium cacodylate buffer (pH 7.4) containing 2% paraformaldehyde (Electron Microscopy Sciences 15710) and 2.5% glutaraldehyde (Electron Microscopy Sciences 16220) ...
-
bioRxiv - Plant Biology 2021Quote: ... The roots of 7 to 10 day old seedlings were collected and fixed for 30 min with 2-(N-morpholino) ethane sulfonic acid (MES) buffer solution pH 5.7 containing 4% paraformaldehyde (32% methanol-free solution; Electron microscopy Sciences). pH was adjusted to 5.7 with KOH solution.
-
bioRxiv - Biochemistry 2020Quote: ... 3 µl of sample (0.5 mg/ml) were applied to freshly glow-discharged (see above) C-flat 2/1-3C-T grids (Electron Microscopy Sciences) and plunged into liquid ethane after 2 s blot time with relative blot force set to 21 ...
-
bioRxiv - Biophysics 2020Quote: ... Approximately 3.2 μL of RNCs were incubated on plasma-cleaned 300-mesh holey carbon grids (C-flat R2/2, Electron Microscopy Science) for 1 min ...
-
bioRxiv - Biophysics 2020Quote: ... A 4% aqueous osmium tetroxide solution (0.1575 M OsO4 in ampules at 2 mL each) was purchased from Electron Microscopy Sciences. 2,2’-Bipyridine 99+% (bipy ...
-
bioRxiv - Cell Biology 2020Quote: ... 3 μl of diluted sample was deposited onto a glow-discharged (Edwards S150B, 30sec, 30-50 mA, 1,2kV, 10-2 mbar) continuous carbon grid (CF400-CU-UL, Electron Microscopy Sciences). After one minute at RT ...
-
bioRxiv - Biophysics 2022Quote: ... then cells were allowed to adhere for 15 min in media prior to chemical fixation in 2% PFA and 0.2% glutaraldehyde (Electron Microscopy Sciences). FActin was stained by permeabilizing cells with 0.1% Triton-X-100 prior to incubation with 3.3 μM phalloidin-AlexaFluor647 (Invitrogen ...
-
bioRxiv - Plant Biology 2021Quote: Marchantia thalli were cut in small pieces (1×2 mm) and fixed immediately in buffered phosphate 0,1 M paraformaldehyde 4% (Electron Microscopy Sciences), glutaraldehyde 2,5% (TAAB Laboratories ...
-
bioRxiv - Neuroscience 2021Quote: Glass vials were prepared prior to the experiments with a 2-mm layer of Sylgard 184 (Electron Microscopy Sciences, Hatfield, PA) at the bottom ...
-
bioRxiv - Neuroscience 2020Quote: ... 20 mM NaCl and incubated with secondary 15 nm gold-tagged goat-anti-rabbit IgG F(ab’)2 (Electron Microscopy Sciences) diluted 1:10 in 20 mM Tris pH 7.4 ...
-
bioRxiv - Cell Biology 2022Quote: ... Samples were taken prior to the experiment (‘Pre’) and at each time point by diluting 50 μl of cells 1:1 with 2% glutaraldehyde (EMS, 16220) and incubating at 4°C to allow cells to sediment ...
-
bioRxiv - Bioengineering 2020Quote: ... bone pieces were washed with double distilled water for 2 minutes and embedded in Epon epoxy resin dissolved in acetone (Embed 812, EMS, USA). The Epon embedding was carried out in 5 steps ...
-
bioRxiv - Immunology 2020Quote: ... slides were washed twice for 5 minutes in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
-
The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... atala eye was immersed for prefixation in 2.5% Glutaraldehyde/2% paraformaldehyde in 0.1M Sodium Cacodylate buffer (pH 7.4) (Electron Microscopy Sciences, PA, USA) for 2h at room temperature ...
-
bioRxiv - Neuroscience 2021Quote: ... Copper grids containing thin sections were post-stained for 20 min in 2% aqueous uranyl acetate (Electron Microscopy Sciences; Cat #22400) and for 5 min in Reynolds lead citrate (Reynolds ...
-
bioRxiv - Physiology 2021Quote: The mouse liver was cut into 2-3 mm3 cubes and immediately immersed in a fixation solution containing 2.5% glutaraldehyde (Electron Microscopy Science) in 0.1 M cacodylate buffer pH 7.4 for 1 hour at room temperature and then overnight at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... The samples were transfer into slicksealTM microcentrifuge tube (National Scientific, CA170S-BP) and fixed with 2% paraformaldehyde (Electron Microscopy Science, Nm15710) in phosphate-buffered saline (PBS ...
-
bioRxiv - Microbiology 2020Quote: ... 3 μL of sample was applied to glow-discharged (30 s) Quantifoil copper grids (R2/2, 200 mesh, Electron Microscopy Sciences), blotted on both sides for 4 s ...
-
bioRxiv - Systems Biology 2023Quote: ... slides were washed twice for 5 minutes in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
-
bioRxiv - Biochemistry 2023Quote: ... was applied four times to glow-discharged Quantifoil R3.5/1 grids coated with a layer of 2 nm ultrathin carbon (Au 400 2nm C, Electron Microscopy Sciences) with 45 seconds incubation followed by a manual blot between each application ...