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Citations for Electron Microscopy Sciences :
551 - 600 of 810 citations for D Iditol 1 13C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... the sample was then placed on a coverslip and treated with Alcian blue 1% (Electron Microscopy Sciences) to improve attachment ...
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bioRxiv - Microbiology 2023Quote: ... 3 µL of the purified CVB5 mutant virus sample at 4.5 mg mL-1 concentration was loaded onto Quantifoil R 1.2/1.3 grids (EMS), that were previously glow-discharged for 30 s in a GloCube Plus device (Quorum Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then rinsed with Milli-Q filtered water and stained with 1% aqueous uranyl acetate (EMS) for 15-30 min ...
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bioRxiv - Physiology 2023Quote: ... stained with DAPI (1 mg/ml in PBS) and mounted with Fluorogel mounting medium (EMS, Hatfield, PA), Immunostaining was visualized by with a Zeiss LSM980 confocal microscope.
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bioRxiv - Neuroscience 2024Quote: ... Brains were then fixed overnight at 4°C in 1% paraformaldehyde (20% PFA, Electron Microscopy Sciences, #15713). Immunohistochemistry was performed as described previously (McFarland et al. ...
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bioRxiv - Biochemistry 2024Quote: ... The assembled complex (4 µL) was applied to C-flat R2/1 holey carbon copper grids (EMS) after glow discharging the grids at 11 mA for 30 sec (Pelco) ...
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bioRxiv - Cell Biology 2024Quote: ... the sections were stained with a 0.5% uranyl acetate and 1% lead citrate solution (both from EMS). The final observation was performed using a Zeiss Leo 906 (Carl Zeiss ...
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bioRxiv - Immunology 2024Quote: ... Cells were rinsed twice in FACS buffer then fixed in 1% PFA (Electron Microscopy Sciences, 15714-5) for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were postfixed for 1 h at 4°C in the dark with 0.1 M Sorensen’s Phosphate Buffer pH 7.4 containing 1% (w/v) osmium tetroxide (Science Services EMS) and 0.8% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... gold EM grids with Quantifoil holey carbon film (Quantifoil, R2/1, 300 mesh, gold; Electron Microscopy Sciences, Q3100AR1 ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5 – 1 x 105 cells were washed with CSM and fixed with 1.6% PFA (Electron Microscopy Sciences) in CSM for 10 min at room temperature (RT) ...
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bioRxiv - Cell Biology 2020Quote: ... Fixed samples were washed three times by the addition of fresh 0.1 M cacodylate buffer (pH 7.2) buffer and post-fixed in 1% osmium (EMS) in 0.1 M cacodylate buffer (pH 7.2 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then post-fixed with 1% OsO4 in Cacodylate buffer (0.1 M, pH 7.2, EMS, Hatfield, PA), rinsed in the same buffer and pelleted ...
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bioRxiv - Neuroscience 2020Quote: ... and 1 μL of virus mixed with Fast Green FCF Solution 2.5% (Electron Microscopy Sciences, Hatfield, PA, USA). The mouse was placed on a heating blanket (37 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... Foreguts were subjected to antigen retrieval in 1× R-universal epitope recovery buffer (Electron Microscopy Sciences 62719-10) for 1 hour at 60–65°C ...
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bioRxiv - Neuroscience 2021Quote: ... animals underwent transcardial perfusion with 0.9% saline and 4% paraformaldehyde solutions in 1×-PBS (15710, Electron Microscopy Sciences). Whole brains and spinal cords were dissected and fixed overnight in 4% paraformaldehyde at 4°C and washed three times in PBS pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... These fragments were embedded in 100% Araldite resin solution (Araldite: DDSA: DMP30; 12:9:1; Electron Microscopy Sciences) and baked overnight at 60°C ...
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bioRxiv - Bioengineering 2021Quote: ... Formvar/carbon-coated copper grids and uranyl acetate (1% solution) for TEM were purchased from Electron Microscopy Sciences. Silicon wafers for scanning electron microscopy (SEM ...
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bioRxiv - Bioengineering 2022Quote: ... followed by approximately 1 L of 4% (w/v) paraformaldehyde (PFA) fixative (19208, Electron Microscopy Sciences, Hatfield, PA) in 1X PBS.
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bioRxiv - Bioengineering 2022Quote: Cells were fixed with 1% (for Piezo1) or 4 % (for phalloidin) paraformaldehyde (PFA, Electron Microscopy Sciences 15713-S) for 10 minutes and washed two times with PBS ...
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bioRxiv - Cell Biology 2022Quote: ... excess culture medium was wicked away from the edges of the coverslip using Whatman #1 filter paper (EMS). Total blotting time did not exceed 5 seconds ...
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bioRxiv - Cell Biology 2024Quote: ... tissue was rinsed in 0.1N acetate buffer (pH 5.0-5.2) at room temperature and then en bloc stained overnight with 1% uranyl acetate (EMS) in acetate buffer at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Temporal bones were immediately removed and fixed via perfusion of 1 ml cold 4% PFA (Electron Microscopy Science) in 1X PBS through the oval and round windows ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 μl of the purified G6PDH-OpcA sample at a protein concentration of approximately 1 mg/ml were applied to glow-discharged C-Flat grids (R1.2/1.3 3Cu-50) (EMS) and directly plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... mouse hindlimbs were fixed in 1 x PBS containing 1.5% glutaraldehyde/1.5% formaldehyde (Electron Microscopy Sciences, Cat#: 15950), 0.05% tannic acid at 4 °C for overnight with gentle agitation ...
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bioRxiv - Plant Biology 2023Quote: Roughly 30,000 Arabidopsis bak1-5 bkk1-1 cerk1-2 (bbc) seeds were mutagenized using 0.2% ethyl methanesulfonate (EMS). Mutagenized M1 seeds were sown on soil and allowed to grow to set seeds ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS for 2h at room temperature and stored in 1% PFA in PBS at 4°C and finally fixed with 2.5% GA and 0.05% malachite green (EMS) in 0.1 M sodium cacodylate buffer ...
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bioRxiv - Biophysics 2023Quote: ... and 1 mM dithiothreitol (DTT) were applied to glow-discharged C-Flat grids (R1.2/1.3 3Cu-50) (EMS) and immediately plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then negatively stained for 1 min with 5 μL of 2% uranyl acetate (Electron Microscopy Sciences, 541-09-3 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were then post-fixed with 1% glutaraldehyde in 0.15 M Sorensen’s phosphate buffer (SPB; Electron Microscopy Sciences) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed 3 times in ddH2O and incubated on ice for 1 hour in a solution composed of 1.5% potassium ferrocyanide and 1.5% osmium tetroxide (EMS) diluted in ddH2O ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were centrifuged and the pellet was resuspended with PBS containing 1% formaldehyde (Electron Microscopy Sciences, cat# 15714) for further crosslinking ...
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bioRxiv - Biophysics 2024Quote: 30μL of polymerized tau sample was deposited onto freshly-cleaved muscovite mica (V-1 quality, Electron Microscopy Sciences) and adsorbed for 3 minutes ...
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bioRxiv - Immunology 2024Quote: ... for 1 min at room temperature and fixed in a solution containing 1.6% Paraformaldehyde (PFA) (Electron Microscopy Science) and cryopreserved in a solution containing 0.5% BSA and 10% dimethyl sulfoxide (DMSO) ...
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bioRxiv - Cancer Biology 2024Quote: Mouse tissue was cut into 1 mm3 pieces and fixed with 2.5% glutaraldehyde solution (EMS, Hatfield, PA, US) in phosphate buffer (PB ...
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bioRxiv - Developmental Biology 2024Quote: ... nuclei were counterstained with Hoechst (1:3000) and the slides were mounted using mounting media (Electron Microscopy Sciences). Primary antibodies used are described in Table S1 and secondary antibodies were Alexa Fluor-conjugated secondary antibodies (1:1000).
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bioRxiv - Neuroscience 2021Quote: ... The antigen retrieval was performed in 1 x Citrate buffer pH 6.0 in ddH2O (Electron Microscopy Sciences, 64142-08). 1 x Citrate buffer pH 6.0 in ddH2O was warmed up to 95°C and applied over samples for 20 min and let it cool at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... This was polymerised for 24 h at 60°C inside a closed gelatin capsule (size 1; Electron Microscopy Sciences) half-filled with previously hardened resin to ensure the insect was situated in the center of the final resin block ...
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bioRxiv - Plant Biology 2020Quote: ... 2.5% in phosphate buffer (PB 0.1 M [pH 7.4]) for 1h at RT and post-fixed in a fresh mixture of osmium tetroxide 1% (EMS) with 1.5% of potassium ferrocyanide (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... 2.5% in phosphate buffer (PB 0.1 M [pH 7.4]) for 1h at RT and post fixed in a fresh mixture of osmium tetroxide 1% (EMS) with 1.5% of potassium ferrocyanide (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... the tissues were washed with ddH2O at room temperature and then placed in the 0.22 µm-Millipore-filtered 1% thiocarbohydrazide (TCH) (#21900 EMS) in ddH2O solution for 20 minutes ...
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bioRxiv - Immunology 2020Quote: Pyroptotic BMDMs were fixed for 1 hour at room temperature (RT) in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, 15710), then incubated with PBS containing 50 mM NH4Cl (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... Tendons of interest were identified by collecting 1 μm sections stained with an epoxy tissue stain (Electron Microscopy Sciences). Ultrathin sections containing tendons of interest were cut at 80 nm ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with 10 nm gold-conjugated goat anti-guinea pig IgG (1:200, Electron Microscopy Sciences, #25329) in blocking buffer for 3 hr ...
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bioRxiv - Cell Biology 2022Quote: ... four to five serial sections were picked up per 2 × 1 mm formvar and carbon coated slot grid (EMS). Because the Aclar coverslip ...
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bioRxiv - Microbiology 2024Quote: ... The samples were then rinsed with 0.1 M cacodylate buffer and fixed with 1 % osmium tetroxide (Electron Microscopy Sciences) in 0.1 M sodium cacodylate buffer for 30 min at room temperature ...
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bioRxiv - Physiology 2023Quote: ... One microliter of this resuspension is added to 1 mL of Mammalian Ringer’s Solution (Electron Microscopy Sciences #11763-10) containing isolated myofibers from the flexor digitorum brevis muscle (FDB ...
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bioRxiv - Biophysics 2023Quote: ... MMA/graphene was applied onto 300 mesh gold-coated Quantifoil R 0.6/1 copper grids (Electron Microscopy Sciences, USA) by using the grids to scoop out the bilayer ...
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bioRxiv - Cell Biology 2024Quote: 1 × 106 cells in HBS were fixed using 4% paraformaldehyde (PFA) (cat no. 15710; Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... 5 μL of sample was applied to a glow discharged QF-R2/1 Cu 200M grid (Electron Microscopy Sciences). Grids were frozen into -185 °C liquid ethane using a Leica EM GP2 automatic plunge freezer equilibrated to 80% humidity and 10 °C with a 10 second sample incubation time and a 2.5 second blot time on Whatman 1 paper ...