Labshake search
Citations for Electron Microscopy Sciences :
5051 - 5100 of 7095 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... 8 µm – 10 µm cryosections were collected on poly-L-lysine coated glass slides (EMS), and unfixed eye cups sections were immediately fixed with 2% PFA for 2 minutes ...
-
bioRxiv - Developmental Biology 2021Quote: ... animals were embedded in epoxy resin (EMS, Hatfield, UK). Ultrathin (65 nm ...
-
bioRxiv - Developmental Biology 2021Quote: ... then 15% sucrose and 50% Tissue Freezing Medium (TFM) (Electron Microscopy Sciences #72592). The fixed and dehydrated glands were frozen in TFM indirectly over liquid nitrogen ...
-
bioRxiv - Neuroscience 2021Quote: ... 2% glutaraldehyde (EMS 16000), p.H 7.4 ...
-
bioRxiv - Biochemistry 2021Quote: ... The sample was applied to glow discharged holey carbon grids which was then back blotted before adding 3 µL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
-
bioRxiv - Immunology 2021Quote: ... The cells were further washed twice with 1x permeabilization buffer (eBioscience) before fixation in 1.6% PFA solution (Electron Microscopy Sciences) containing 125 nM Iridium (Fluidigm ...
-
bioRxiv - Neuroscience 2021Quote: All animals were trained in sound-attenuating behavioral boxes (ENV-018MD-EMS: Med Associates). A light-pipe lickometer was located on one side of the box ...
-
bioRxiv - Neuroscience 2021Quote: ... At room temperature the samples were post fixed in 1% osmium tetroxide (EMS 19100) for 1 hour ...
-
bioRxiv - Physiology 2021Quote: ... Cells were then incubated with the DNA intercalator Iridium (Fluidigm, 201192A) at a dilution of 1:4,000 in 2% paraformaldehyde (Electron Microscopy Sciences, 15714) in DPBS (Corning ...
-
bioRxiv - Microbiology 2021Quote: ... EV sample (4 μL) was applied to glow discharged Lacey grids (S166-3, EMS) prior plunge-freezing (EMGP ...
-
bioRxiv - Developmental Biology 2021Quote: ... IL2: klp-6pro:GFP) were mutagenized for 4 h at room temperature in 70 mM ethyl methansulfonate (EMS, Sigma). Nonclonal F2 progeny were visually inspected for altered cellular morphologies using a Nikon SMZ1500 stereomicroscope with an HR Plan Apo 1.6× objective ...
-
bioRxiv - Developmental Biology 2021Quote: Micropatterned coverslips were fixed with 4 % paraformaldehyde (Electron Microscopy Sciences 15713) in warm medium for 30 min ...
-
bioRxiv - Biochemistry 2021Quote: ... samples were mounted to a metal disc using thermally conductive copper tape (Electron Microscopy Sciences, Hatfield, PA, USA), primed in a 55 °C oven for 2 mins ...
-
bioRxiv - Biochemistry 2021Quote: ... Clearium mounting medium was purchased from Electron Microscopy Sciences (Hatfield, PA, USA).
-
bioRxiv - Biochemistry 2021Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... We used separate surgical micropunches (EMS Rapid Core Instruments) to punch out the three brain regions of interest from 100□μm slices ...
-
bioRxiv - Biochemistry 2021Quote: ... sarkosyl and 4 μl of the suspension was applied directly to a glow-discharged C-flat™ Holey Carbon CF-2/2-4C Cu 400 mesh cryo-EM grid (Electron Microscopy Sciences) in the chamber of the Leica GP2 plunging robot ...
-
bioRxiv - Biochemistry 2021Quote: ... they were rinsed three times with PBS and fixed with 4 % PFA (Electron Microscopy Sciences) for 20 min at room temperature ...
-
bioRxiv - Biochemistry 2021Quote: ... Samples of the complex (2 μL) were applied to glow-discharged (1 min, easiGlow, Pelco) R2/2 300-mesh Quantifoil holey carbon grids (Electron Microscopy Sciences). The grids were blotted for 2 s with Whatman 41 filter paper and flash-frozen by plunging into liquid ethane using a Leica EM CPC manual plunger ...
-
bioRxiv - Biochemistry 2021Quote: ... the DACR clone #A4 at early passage was mutagenized again (0.3 mg/ml EMS for 72 h). After recovery ...
-
bioRxiv - Biochemistry 2021Quote: 100.106 haploid HAP1 cells at 60 % confluency were treated for 72 h with 0.3 mg/mL ethyl methanesulfonate (EMS, Sigma-Aldrich) directly added to the cell medium ...
-
bioRxiv - Biochemistry 2021Quote: ... 2 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 1/4 300 mesh Cu, Electron Microscopy Sciences) followed by plunging into liquid ethane using an FEI Vitrobot Mark IV set to a blot time of 22 and a blot force of 22 for QYNNENNFV and 24 for GSTVYAPFT ...
-
bioRxiv - Biochemistry 2021Quote: ... 1.8 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 2/1 200 mesh Cu, Electron Microscopy Sciences) and plunge frozen into liquid ethane using a FEI Vitrobot Mark IV using a blot time of 25-30 seconds and a blot force of 22 ...
-
bioRxiv - Biochemistry 2021Quote: ... 2 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 2/1 200 mesh Cu, Electron Microscopy Sciences) and plunge frozen into liquid ethane using a FEI Vitrobot Mark IV ...
-
bioRxiv - Biochemistry 2021Quote: ... An UltrAuFoil grid (1.2/1.3-µm, 300 mesh, Electron Microscopy Sciences, catalog no. Q350AR13A) was glow-discharged in a PELCO easiGlow for 15 s at 25 mA ...
-
bioRxiv - Biochemistry 2021Quote: Cells were plated on micro cover glasses (Electron Microscopy Sciences 72226-01). After confluence ...
-
bioRxiv - Biochemistry 2021Quote: ... UltrAuFoil R 1.2/1.3 300 mesh grids (Electron Microscopy Sciences, Q350AR13A) were rendered hydrophilic by pretreatment with a O2/H2 gas mixture using a Solarus 950 plasma cleaning system (Gatan ...
-
Cryo-EM reveals disrupted human p97 allosteric activation by disease mutations and inhibitor bindingbioRxiv - Biochemistry 2021Quote: ... Protein vitrification was achieved using the Leica GP2 plunge freezer on Quantifoil (R1.2/1.3, 200 or 300 mesh-Cu) holey carbon grids (Electron Microscopy Sciences, PA, USA). Firstly ...
-
bioRxiv - Biochemistry 2021Quote: ... purchased from Electron Microscopy Sciences, (Hatfield ...
-
bioRxiv - Biochemistry 2021Quote: ... in the presence of 1 mM ATPγS for 5 min prior to Vitrobot freezing on 300 mesh R 2/2 Quantifoil copper grids (Electron Microscopy Sciences). All three datasets were collected on a FEI Titan Krios TEM with a GATAN post column energy filter (20 eV zero-loss filtering ...
-
bioRxiv - Neuroscience 2021Quote: ... and a layer of transparent nail polish (Electron Microscopy Sciences, Hatfield, PA) was applied and allowed to cure for 10 – 15 min ...
-
bioRxiv - Biochemistry 2021Quote: ... The grids were then rotary shadow cast with 80 % platinum – 20 % palladium (EMS sciences inc) followed by an overlayer of carbon.
-
bioRxiv - Biochemistry 2021Quote: ... cells were washed with 1X PBS and fixed in 4% paraformaldehyde (PFA) solution (diluted from 16% PFA, Electron Microscopy Sciences) in PBS for 15 min ...
-
bioRxiv - Microbiology 2021Quote: ... 50 ml of lake water was fixed with paraformaldehyde (final concentration 1%, without methanol, Electron Microscopy Sciences) overnight at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... and samples were deposited on a TEM Carbon coated grids (80 mesh square grid, EMS; Ted Pella, Inc., Redding, CA, USA) and incubated for 2 min ...
-
bioRxiv - Immunology 2021Quote: ... cells were washed and fixed at 4°C for 10 min in 4% paraformaldehyde (Electron Microscopy Sciences). The fixed cells were washed and resuspended in PBS containing 5% FBS ...
-
bioRxiv - Microbiology 2021Quote: ... approximately 4 μL of purified virus sample was applied to a glow-discharged 300-mesh copper grid coated with continuous carbon film (Electron Microscopy Sciences, Hatfield, PA). The grid was plunged into a liquid ethane container that is further cooled by a liquid nitrogen bath ...
-
bioRxiv - Microbiology 2021Quote: Four μL of purified virus was applied to a Formvar- and carbon-coated 400-mesh copper grid (Electron Microscopy Sciences, Hatfield, PA) for 25 seconds ...
-
bioRxiv - Developmental Biology 2021Quote: ... Incisors were sagittally embedded in a hard-formulation epoxy embedding medium (EpoFix, EMS, Hatfield, PA, USA). Samples were ground and polished with an EcoMet 30 grinder polisher (Buehler ...
-
bioRxiv - Developmental Biology 2021Quote: ... We collected unfertilized oocytes and embryos in M2 medium and fixed them in 4% paraformaldehyde (Electron Microscopy Sciences) 1x PBS for 30 min at room temperature ...
-
bioRxiv - Cell Biology 2021Quote: ... 10-μm lightly fixed3 pancreas sections were mounted onto 22x22 mm glass coverslips (Electron Microscopy Sciences) coated in 0.1% Poly-L-lysine (Sigma ...
-
bioRxiv - Developmental Biology 2021Quote: ... slides were coverslipped using Fluoromount G (Electron Microscopy Sciences, USA). For Lhx1 staining ...
-
bioRxiv - Developmental Biology 2021Quote: ... whole globes dissected from embryonic 13-LGS were immersed in freshly prepared cold 4% paraformaldehyde (Electron Microscopy Sciences, USA) in 0.1M sodium phosphate buffer ...
-
bioRxiv - Biochemistry 2021Quote: Samples (4 μL) were applied to glow-discharged continuous carbon film supported copper grids (CF300-CU-50 grids, Electron Microscopy Sciences). After 1 min incubation ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... stained with uranyl acetate (Electron Microscopy Sciences) and lead citrate ...
-
bioRxiv - Neuroscience 2021Quote: ... with a final dehydration in propylene oxide for 5 min at room temperature prior to resin infiltration with a 1:1 mixture of propylene oxide and EPON (Embed-812, 14121, Electron Microscopy Sciences, Hatfield, PA, USA) for 1 h and two fresh replacements of 100 % EPON (first for 1 h ...
-
bioRxiv - Neuroscience 2021Quote: ... the samples were covered with BEEM capsules (70000, Electron Microscopy Sciences, Hatfield, PA, USA) filled with fresh EPON resin and cured in an oven at 60 °C for 48 h ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Samples containing purified MCPs were applied and fixed onto 400 mesh Formvar-coated copper grids (EMS Cat# FF400-Cu). Fixation was done using 2% (v/v ...
-
bioRxiv - Zoology 2021Quote: ... specimens were dehydrated and embedded in Epon/Araldite epoxy embedding media (Electron Microscopy Science, 13940) according to the standard protocol previously described12,75.
-
bioRxiv - Zoology 2021Quote: ... sponge tissues were fixed for 2 hours at 4 °C with 2.5% glutaraldehyde (Electron Microscopy Science, 16020) and post-fixed for 1 hour at room temperature (RT ...