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Citations for Electron Microscopy Sciences :
451 - 492 of 492 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... VLP samples were fixed with 2% (v/v) glutaraldehyde in water solution on 400 mesh Formvar-coated copper grids (EMS Cat#FF400-Cu). After fixation ...
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bioRxiv - Cancer Biology 2023Quote: 3.105 cells were seeded onto glass slides and fixed for 12min at room temperature with a solution of 4% paraformaldehyde (PFA, Electron Microscopy Sciences, 15710) diluted in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... dehydrated in a graded series of ethanol ranging from 30% to absolute solution and embedded in resin (EMBed-812, Electron Microscopy Sciences, USA). 80 nm thick sections from at least four blocks from Ctr iPSC-derived MYOrganoids in presence or absence of Ctr fibroblasts were stained with uranyl acetate and lead citrate ...
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bioRxiv - Cell Biology 2024Quote: ... The filters were then removed from the culture dishes and incubated overnight in 4% paraformaldehyde solution at 4 °C (Electron Microscopy Sciences #15713). In preparation for paraffin embedding and sectioning ...
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bioRxiv - Neuroscience 2024Quote: ... were deeply anesthetized by isoflurane and transcardially perfused with ice cold 1X PBS (∼5–7 ml) followed by 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences: 15713) in 1X PBS (∼5–10 ml ...
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bioRxiv - Neuroscience 2024Quote: Three weeks after cervical AAV injection and one to fourteen days after sham or intracortical injury animals underwent transcardial perfusion with 0.9% saline and 4% paraformaldehyde (PFA) solutions in 1× phosphate-buffered saline (PBS) (15710, Electron Microscopy Sciences, Hatfield, PA). Whole brains were dissected and fixed overnight in 4% PFA at 4°C and washed three times in PBS pH 7.4 ...
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bioRxiv - Physiology 2024Quote: ... Hearts were perfused for 3 min with PBS at 37°C to wash out the blood followed by a fixative solution of Glutaraldehyde 3% in 0.1M Cacodylate buffer pH7.4 (Electron Microscopy Sciences-cat#16538-15) for less than 5 min ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were initially washed with 1X Assay buffer (GOLGI-ID kit) and then fixed with 4% of 16% paraformaldehyde solution (Electron Microscopy Sciences, 15710) at room temperature for 10 minutes before staining the cells for detection of Golgi bodies using the manufacture’s manual for “Staining Adherent Fixed Cells” ...
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bioRxiv - Biophysics 2024Quote: Cryo-EM samples of concentrated peptide solutions were prepared on either 300 mesh 2/2 Quantifoil or C-flat EM grids (Electron Microscopy Sciences, USA), on which 10 nm protein A-conjugated colloidal gold particles (Au–NP ...
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bioRxiv - Cell Biology 2024Quote: Primary neurons or astrocytes cultured on glass coverslips at the same plating density as was used for live cell imaging experiments were fixed for 10 minutes at 37°C/5% CO2 in a solution containing 2% paraformaldehyde (PFA; Electron Microscopy Sciences 15713) and 0.05% glutaraldehyde in PHEM buffer (EMS ...
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bioRxiv - Immunology 2021Quote: ... was added to anti-CD16/32 solution at a 1 : 100 dilution for dead cell exclusion and fixed with 4% paraformaldehyde (15710, Electron Microscopy Sciences, Lucerne, Switzerland) in PBS for 20 min on ice after staining.
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bioRxiv - Neuroscience 2022Quote: ... specimens were dehydrated using serially graded ethanol solutions (30%, 50%, 70%, 90% and 100%) and infiltrated and embedded in Spurs resin (Electron Microscopy Sciences, Hatfield, PA) following the manufacturer’s recommendations ...
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bioRxiv - Systems Biology 2022Quote: ... 6F- Volume was completed to 8 ml with room temperature 1 X PBS and then 500 μl of 16 % PFA solution (Electron Microscopy Sciences, cat. # 15710) was added ...
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bioRxiv - Cell Biology 2021Quote: ... The sections are again washed thrice in PBS and stained with DAPI for 5 minutes and mounted with Fluoromount-G solution (17984-25, Electron Microscopy Sciences, Pennsylvania, USA). Using a Zeiss Axio Imager Z1 ApoTome Microscope ...
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bioRxiv - Neuroscience 2021Quote: ... and tran-scardially perfused with 20ml of 0.01M phosphate buffered saline (1X PBS) followed by 20ml of 4% paraformaldehyde (diluted from a 32% aqueous paraformaldehyde solution with PBS; Electron Microscopy Sciences [EMS], Hatfield, Pennsylvania) at a flow rate of 10-14ml/min ...
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bioRxiv - Neuroscience 2023Quote: ... rats were transcardially perfused with an isotonic solution at 9.25% in distilled water as a prewash and 4% paraformaldehyde (PFA; fixative, Cat No. 15714-S, Electron Microscopy Sciences, Hatfield, PA, USA) using the Perfusion Two TM Automated Pressure Perfusion System (Leica Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with heparin (12.5 U/ml) followed by 25 ml RT fixative solution consisting of 4% PFA and 2.5% glutaraldehyde (both Electron Microscopy Science, Hatfield, PA, USA) in PBS at a flow rate of 2 ml/min ...
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bioRxiv - Developmental Biology 2024Quote: ... and pancreas were harvested at the experimental endpoint and immediately fixed in 4% paraformaldehyde in 1X PBS (16% solution, Electron Microscopy Sciences, cat# 15711) at 4°C overnight ...
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bioRxiv - Systems Biology 2023Quote: ... Slides were washed twice for 5 min in wash buffer and fixed for 5 min in diluted glutaraldehyde solution 2% (Electron Microscopy Sciences, Hatfield, PA) in PBS-low barium (Ionpath ...
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bioRxiv - Cell Biology 2022Quote: Four μm kidney cortices optimal cutting temperature (OCT) compound embedded sections were incubated with 100 μl freshly prepared Oil Red O solution (Electron Microscopy Science, Hatfield, PA) for 15 minutes and counterstained with Hematoxylin Harris solution (VWR ...
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bioRxiv - Neuroscience 2024Quote: ... Cat# 70011044) and fixation with 4% paraformaldehyde (PFA) (dilution 1:4; Paraformaldehyde 16% Aqueous Solution EM Grade, Electron Microscopy Sciences, Hatfield, PA; Cat# 15710) were performed 6 hours after saline or LPS injection ...
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bioRxiv - Neuroscience 2024Quote: ... samples were incubated in a 2% OsO4 in 0.15 M cacodylate buffer solution containing 2 mM calcium chloride (19152, Electron Microscopy Sciences, Hatfield, PA, USA) for 2 hours in the dark ...
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bioRxiv - Bioengineering 2024Quote: ... A 3 µL droplet of the D-GQD solution (5 µM) was placed on the carbon-coated copper TEM grid (Purchased from Electron Microscopy Sciences; PA, USA), and air-dried ...
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bioRxiv - Cell Biology 2024Quote: ... fixed for 20 min in 4% EM-grade paraformaldehyde (PFA) diluted in phosphate-buffered saline (PBS) from an 8% PFA Aqueous Solution (Electron Microscopy Sciences, 157-8) and blocked for 1 h in PBS plus 0.2% Triton X-100 and 4% normal goat serum (NGS ...
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bioRxiv - Biophysics 2020Quote: ... 5 uL of DNA origami solution (5 nM) was deposited onto fresh glow discharged carbon film with copper grids (CF200H-CU; Electron Microscopy Sciences Inc., Hatfeld, PA), and the sample was then allowed to absorb onto the surface for 30s ...
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bioRxiv - Genetics 2020Quote: ... Then the cells were counted and diluted to 106 cells/ml and fixed with 4% paraformaldehyde aqueous solution (Electron Microscopy Sciences #15710, Formaldehyde methanol-free) for 10 minutes at room temperature (RT) ...
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bioRxiv - Neuroscience 2022Quote: Postnatal mouse were transcardially perfused with 15ml (P14) or 25ml (>P21) of 2% PFA freshly prepared from 32% PFA solution (Electron Microscopy Sciences, 50-980-495). Perfused brains were dissected out ...
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bioRxiv - Developmental Biology 2021Quote: ... The eye imaginal discs were dissected in cold 1X solution of Phosphate Buffered Saline (PBS) and fixed using 4% p-formaldehyde (Electron Microscopy Sciences, Catalog no. 15710). Fixed tissues were washed using PBS-T [0.2% Triton X-100 (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... the cultured neurons were fixed for 10-15 min by transferal of the coverslip into a dish with 4% paraformaldehyde (16% aqueous solution, 15710; Electron Microscopy Sciences, Hatfield, Pennsylvania, USA) and 0.2% glutaraldehyde (25% aqueous solution ...
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bioRxiv - Bioengineering 2020Quote: Animals were implanted with a stainless-steel probe or a Fiber F1/F2 for 4 weeks and then anesthetized with a ketamine (20 mg/mL)/xylazine (2 mg/mL) solution and transcardially perfused with phosphate-buffered saline (PBS) (Fisher BP661-10) followed by 4% paraformaldehyde (PFA) (Electron Microscopy Sciences Cat. #15714-S) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... and tran-scardially perfused with 20ml of 0.01M phosphate buffered saline (1X PBS) followed by 20ml of 4% paraformaldehyde (diluted from a 32% aqueous paraformaldehyde solution with PBS; Electron Microscopy Sciences [EMS], Hatfield, Pennsylvania) at a flow rate of 10-14ml/min ...
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bioRxiv - Neuroscience 2021Quote: ... cultures were washed in imaging buffer to remove non-internalised HRP and fixed with a solution of 2 % glutaraldehyde (Electron Microscopy Sciences, Hatfield, USA, 16019) in phosphate buffered saline ...
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bioRxiv - Neuroscience 2023Quote: ... cultures were washed in imaging buffer to remove non-internalised HRP and fixed with a solution of 2 % glutaraldehyde (Electron Microscopy Sciences, Hatfield, USA, 16019) and 2% PFA in 0.1 M in phosphate buffer (PB) ...
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bioRxiv - Neuroscience 2023Quote: ... the coverslips were rinsed with 1X PBS and then fixed with freshly prepared 4% paraformaldehyde (from 32% Paraformaldehyde Solution, Electron Microscopy Science Cat# 15714-S) for 30 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... A portion of each EV sample (10 μl) was fixed in a 1:1 solution using 4% EM-grade paraformaldehyde (Electron Microscopy Sciences, 157-4-100) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The 2 ml of EMS supplemented M9 buffer was added to the 2 ml worm suspension (final concentration of EMS in solution is ∼50 mM). The worms were incubated for 4 hours at room temperature constantly mixing on a nutator ...
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bioRxiv - Neuroscience 2024Quote: mice were perfused 7 dpI and 10 dpl with 25 mL of 2% PBS-paraformaldehyde freshly prepared from 32% PFA solution (Electron Microscopy Sciences, 50-980-495). Perfused brains were dissected out ...
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bioRxiv - Developmental Biology 2022Quote: ... with commercial antigen unmasking solution (VECTOR, Catalog #: H-3300) and underwent immunostaining procedure using the Sequenza™ Immunostaining Center System (Electron Microscopy Sciences, Catalog #: 71407-01)(Zhao et al. ...
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bioRxiv - Neuroscience 2020Quote: ... The midbrain and eyestalk neural tissue was dissected free from the exoskeleton and its enveloping sheath in ice-cold chromating solution comprising 1 part 25% glutaraldehyde (Electron Microscopy Sciences; Cat# 16220; Hatfield, PA), 5 parts 2.5% potassium dichromate (Sigma Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... 50 uL of the gelation solution was added to the samples before immediately transferring them to silicone incubation chambers (Electron Microscopy Sciences, Cat. No. 70324-05) and sealing with a glass microscope slide ...
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bioRxiv - Neuroscience 2020Quote: ... and then deeply anesthetized by Fatal-plus injection (0.075 mL of 97.5 mg/mL solution in NaCl) followed by a standard transcardial perfusion using PBS (50 mL) followed by PFA (Electron Microscopy Sciences, 50 mL, 4% in PBS).
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was discarded and the pellet was fixed in 2.5% glutaraldehyde and 4% PFA (16% stock solution, EM grade, Electron Microscopy Sciences in 0.1 M PIPES (pH 7.2)) for minimum 2h ...