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Citations for Gold Biotechnology :
51 - 100 of 145 citations for Tripartite Motif Containing Protein 55 TRIM55 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... NGM dishes containing 50 µg/mL ampicillin (A-301-25, Gold Biotechnology, St. Louis, MO) and 1 mM IPTG (isopropyl-β-D-thiogalactoside ...
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bioRxiv - Biophysics 2023Quote: ... The bacteria cells were lysed in buffer containing 50 mM HEPES pH 7.4 (#H400, Goldbio), 1.5 M NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Amersham) and protein expression induced with 1 mM final IPTG (Goldbio) for 4 hr at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The supernatant was filtered and passed over Protein A resin (GoldBio), washed with 10 mM sodium phosphate pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was purified using glutathione agarose resin (Gold Biotechnology Inc.). The protein was subjected to TEV cleavage to remove the GST tag and then further purified using a Mono Q 5/50 GL anion exchange column (GE Healthcare Life Sciences) ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then incubated on ice with serum free media containing 10 nM EGF (Gold Biotechnology) for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... the medium was replaced with fresh medium containing 25 μg/ml d-luciferin (GoldBio LUCK-2G). For the luminescence-based readouts of 3D assays ...
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Transcriptional Isoforms of NAD+ Kinase regulate oxidative stress resistance and melanoma metastasisbioRxiv - Cancer Biology 2023Quote: ... mice were injected intraperitoneally with 100μL of DPBS containing D-luciferin monopotassium salt (40μg/mL, Goldbio) 5 min before imaging ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were injected intraperitoneally with 100µL of DPBS containing D-luciferin monopotassium salt (40µg/mL, Goldbio) 5 min before imaging ...
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bioRxiv - Biochemistry 2022Quote: ... The digested protein was passed over a 2-mL Ni-HTC (GoldBio) gravity column to separate the tag-free NnlA ...
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bioRxiv - Immunology 2021Quote: ... The cleaved product was incubated with 1mL of Protein A resin (GoldBio) per 10mg of initial IgG and incubated at room temp for 1 hr to bind any uncleaved IgG and digested Fc ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Bacterial cells were grown in LB broth Miller (Difco) containing chloramphenicol (25 μg/mL) (Gold Biotechnology, USA) at 37 °C to OD600 = 1.7 ...
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bioRxiv - Plant Biology 2023Quote: ... The primary transformants were screened on ½ MS plates containing 15 µg ml-1 hygromycin B (Gold Biotechnology). After 2 weeks ...
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bioRxiv - Microbiology 2023Quote: ... Media containing reduced L-cysteine or reduced glutathione were prepared fresh and supplemented with 2mM TCEP (Goldbio) prior to experimentation ...
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bioRxiv - Microbiology 2020Quote: ... clarified cell lysates were purified by protein G affinity chromatography (Gold Biotechnology, Inc.). The b12 Fab ...
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bioRxiv - Immunology 2020Quote: ... The RBD was purified using protein A agarose resin (GoldBio, Cat# P-400) and cleaving the Fc domain using HRV3C protease (made in house ...
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bioRxiv - Biochemistry 2019Quote: ... centrifuged and resuspended in buffer (1XHBSS and 20mM HEPES, pH 7.4) containing 0.5mg/ml luciferin (LUCNA-1G/GOLDBIO). The cells were then seeded in a 96 well plate at a density of 6 × 104 cells /100μl/ well ...
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bioRxiv - Cell Biology 2021Quote: ... Linear fragments were transformed into BY4742 and selected on YPD agar containing nourseothricin sulfate (100 μg/ml, GoldBio).
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bioRxiv - Synthetic Biology 2022Quote: ... The medium was then changed to PBS containing 100 μg/mL of D-luciferin (Gold Biotechnology, LUCNA-500) to obtain luminescence images of firefly luciferase ...
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bioRxiv - Plant Biology 2022Quote: ... ~0.5cm root tips were harvested from 1000-3000 roots and placed into a 35mm petri dish containing a 70 μm cell strainer and 4.5mL enzyme solution (1.5% [w/v] cellulase [ONOZUKA R-10, GoldBio] ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were injected intraperitoneally with 100 μl of PBS containing D-luciferin monopotassium salt (40 mg/ml) (Goldbio) and mice were anaesthetized with isoflurane 2 min before imaging ...
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bioRxiv - Microbiology 2021Quote: ... 200 uL of the suspension was plated on selective LB agar plates containing nourseothricin (GoldBio, St Louis, MO) at multiple two-fold doubling dilution concentrations and incubated for 48-72 hours at 35°C in ambient air until mutant colonies became visible ...
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bioRxiv - Molecular Biology 2023Quote: ... spread on selective Luria broth (LB) agar plates containing 50 μg/mL carbenicillin (GoldBio Cat #: C-103-25), and grown in a 37 °C incubator overnight ...
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bioRxiv - Microbiology 2024Quote: ... The cells were grown in Luria Broth Media (Research Product Internationals) containing 50 µg/mL Kanamycin (Gold Biotechnology) at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... expression of recombinant protein was induced with 0.5 mM isopropyl β-D-thiogalactoside (GoldBio). For purification of PABPC1 and ePAB ...
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bioRxiv - Biochemistry 2020Quote: ... Induction of protein expression was induced by addition of 1 mM IPTG (Gold Biotech) and cells were grown overnight at 18°C ...
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bioRxiv - Biochemistry 2022Quote: ... protein expression was induced with 1 mM IPTG (isopropyl β-D-1-thiogalactopyranoside; GoldBio) followed by expression for 17 hr at 25°C ...
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bioRxiv - Biochemistry 2022Quote: ... followed by protein induction with 0.5 mM isopropyl ß-D-1-thiogalactopyranoside (Gold Biotechnology) at 18°C for 16 hrs ...
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bioRxiv - Biochemistry 2023Quote: ... The pellet was resuspended in a solubilization buffer containing 2% n-Dodecyl-B-D-Maltoside (DDM, GoldBio, Cat. # DDM10), 350 mM NaCl in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... with all purification buffers for full-length WT NEMO also containing 5 mM Tris(2-carboxyethyl)phosphine (TCEP) (GoldBio) to minimize the formation of disulfide0bonded aggregates ...
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bioRxiv - Biochemistry 2023Quote: ... ten-fold serial dilutions were plated onto synthetic complete agar lacking histidine and containing 0.1% w/v 5-fluoroorotic acid (GoldBio). Plasmid shuffling experiments involving Use1 were conducted in an analogous manner ...
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bioRxiv - Plant Biology 2023Quote: ... containing 30 µL of 300 mg/mL timentin (final concentration of 300 mg/L) (T-104-25, Goldbio, USA), followed by homogenization with a T18 digital ULTRA TURRAX homogenizer (IKA ...
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bioRxiv - Neuroscience 2023Quote: ... media was exchanged in each well for 30 μL of Assay Buffer containing 1.2 mM of D-Luciferin (GoldBio). Cells were incubated for at least one hour at 37°C before the addition of 15 μl of compounds diluted in series in Assay Buffer ...
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bioRxiv - Biochemistry 2023Quote: ... the clarified supernatant containing RPA4AZP was applied to a 3 mL pre-equilibrated Ni2+-NTA (Gold Biotechnology Inc., USA) gravity column ...
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bioRxiv - Biophysics 2019Quote: ... protein expression was then induced with Isopropyl-β-D-1-thiogalactopyranoside 0.1 mM (GoldBio, Canada) for 12 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were clarified using centrifugation and proteins were purified via nickel agarose beads (Gold Biotechnology), eluted with 300mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... along with 2.5 μl of a pre-stained protein ladder (#P008; GoldBio, St. Louis, MO), and separated on an SDS-polyacrylamide gel ...
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bioRxiv - Biochemistry 2021Quote: ... Lipin 1 and lipin 1 ΔM-Lip proteins were purified using Ni-NTA resin (GoldBio). Eluted protein was applied to Streptactin-XT resin equilibrated with equilibration buffer (100 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... All GST fusion proteins were purified using Glutathione Agarose 4B (Gold Biotechnology, #G-250-50), and eluted with HEPES buffered saline (HBS ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were resuspended in 3ml assay buffer (HBSS buffer with 0.01% BSA and 5mM HEPES, pH 7.4) containing 10μM coelenterazine (GoldBio; cat. no. CZ05) at final concentration ...
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bioRxiv - Biophysics 2022Quote: ... A transformant colony was used to inoculate Luria–Bertani (LB) media (Fisher Bioreagents) containing 0.1 mg/mL ampicillin (Gold Biotechnology), which was grown overnight (∼15 h ...
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bioRxiv - Developmental Biology 2021Quote: ... containing 5 mL of digestion buffer (1 mL of Dispase [Corning, 354235], 50 μL of DNase [5 mg/mL, GoldBio, D-301] ...
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bioRxiv - Biochemistry 2021Quote: ... cells were harvested and resuspended in assay buffer containing D-luciferin (0.5 mg ml−1, GoldBio, Cat. no. LUCNA-1G) in 1X HBSS ...
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bioRxiv - Biophysics 2019Quote: ... A dense overnight culture from a single transformant was used to inoculate 10 mL of LB broth (LabExpress) containing 0.1 mg/mL ampicillin (Gold Biotechnology) to a starting Abs600nm of 0.0375 ...
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bioRxiv - Cancer Biology 2021Quote: Commensal dysbiosis was initiated in mice by orally gavaging mice daily for 14 days with 100 μl of an antibiotic cocktail containing vancomycin (0.5 mg/ml, Gold Biotechnology), ampicillin (1 mg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5% Triton with 500 μM each of potassium ferrocyanide and potassium ferricyanide and infiltrated with wash buffer as above containing 1 mM X-Gluc (GoldBio). Samples were incubated at 37°C in the dark until staining became visible and were cleared with ethanol for imaging ...
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bioRxiv - Microbiology 2022Quote: For the lacP1 measurements strains containing the reporter plasmid were grown on LB agar plates with 400 μg/ml Carbenicillin (GoldBio) at 37°C to obtain single colonies ...
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bioRxiv - Biochemistry 2022Quote: ... #DF0446-07-5) or 7H10 (Difco, cat# DF0627-17-4) plates containing 50 ug/mL hygromycin (GoldBio, cat. #H-270) and incubated at 37° C for 3-5 days ...
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bioRxiv - Biophysics 2023Quote: ... resuspended in the assay buffer (5mM HEPES pH 7.4, 1X HBSS and 0.01% w/v BSA) containing 10µM of coelenterazine (GoldBio, Cat. no: CZ05) and seeded in 96-well white plates (SPL Life Sciences ...