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Citations for Gold Biotechnology :
1 - 50 of 73 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... supplemented with 100 µM biotin (GoldBio) under 8% CO2 at 37 °C and used for all protein expressions ...
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bioRxiv - Biochemistry 2023Quote: ... D-biotin was purchased from GoldBio. All solvents and other liquid reagents were purchased from Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... The beads bound with biotinylated cell surface proteins were resuspended in 50 mM Tris (pH 8.5) + 4 M urea + 10 mM TCEP (Gold Biotechnology, TCEP10) and 20 mm IAA (VWR ...
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bioRxiv - Microbiology 2021Quote: ... TRIS (Goldbio, cat. T-400-500), EDTA (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Tris-HCl was purchased from Goldbio (cat. #T-400-5). Urea was purchased from Invitrogen (Carlsbad ...
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bioRxiv - Cancer Biology 2021Quote: ... Blocking was performed in TBS-T with 5% BSA (Gold Biotechnology) for 1h at room temperature ...
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bioRxiv - Genomics 2020Quote: ... Standard 96-well plate medium was used for all camera runs (1X Vogel’s Salts, 0.03% glucose, 0.05% arginine, 1.5% agar, 50 ng/ml biotin, and 25 μM luciferin from GoldBio # 115144-35-9). Liquid cultures were started from fungal plugs as described (Chen et al. ...
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bioRxiv - Cell Biology 2023Quote: PIP Strips (Echelon) were blocked overnight in PBS-T with 3% fatty acid-free bovine serum albumin (GoldBio) at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Clarified cell supernatant containing recombinant antibodies was passed over Protein A Agarose (Goldbio Cat# P-400-5), followed by extensive washing with PBS ...
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bioRxiv - Plant Biology 2023Quote: ... containing 30 µL of 300 mg/mL timentin (final concentration of 300 mg/L) (T-104-25, Goldbio, USA), followed by homogenization with a T18 digital ULTRA TURRAX homogenizer (IKA ...
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bioRxiv - Immunology 2022Quote: ... and antibody was purified using protein A agarose chromatography (Goldbio). Sequences were obtained from PCR reaction products and annotated using the ImMunoGeneTics (IMGT)/V- QUEST database (http://www.imgt.org/IMGT_vquest/)51,52 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in 2 % BSA (A-420-500 GoldBio) in TBS-T to the indicated concentration and incubated with membranes overnight at 4C with rocking ...
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bioRxiv - Cell Biology 2021Quote: ... 200 µl diluted cells (2 x104 cells) and 50 µl (8 mg/ml) D-luciferin (GoldBio, USA) were loaded into each well ...
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bioRxiv - Immunology 2022Quote: ... 4.5 mg E1D1 antibody was incubated with 1 mL Protein A resin (GoldBio, P-400) with rotation at room temperature for 30 minutes and then washed thoroughly with PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were resuspended with 1% low-melt agarose (GoldBio) in PBS at 40°C at ~30 million cells/mL (Fig ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were resuspended with 1% low-melt agarose (GoldBio) in PBS at 40°C at concentrations of ∼15 million cells per milliliter and then loaded into wells of pre-formed phantoms consisting of 1% molecular biology-grade agarose (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were selected in 200µg/mL hygromycin B (Gold Biotechnology), colonies isolated with trypsin-EDTA-soaked filter papers and expanded ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were cultured in media containing 0.1mM D-luciferin (Goldbio) and placed under photomultiplier tubes (PMTs ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... Cell lysate was clarified by centrifugation and glutathione agarose resin (GoldBio) was added to affinity purify the GST-GGA3 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were selected using 200 μg/ml hygromycin B (Gold Biotechnology), and single colonies were isolated using trypsin-soaked filter paper ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were induced with various IPTG concentrations (GoldBio, Cat. # 2481C) at 37°C for 3.5 hours ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were selected in 200 μg ml−1 Hygromycin B (Gold Biotechnology) and colonies were isolated and expanded ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then resuspended in 20 μl 1% low-melt agarose (GoldBio) in PBS at 40°C and loaded into the wells of preformed 1% agarose (Bio-Rad ...
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bioRxiv - Genomics 2021Quote: ... and then stained with the indicated antibody in FACS buffer (PBS with 1% FBS, 10 U/ml DNAse I, GoldBio, St. Louis MO) for 30 minutes on ice with gentle agitation ...
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bioRxiv - Microbiology 2020Quote: ... clarified cell lysates were purified by protein G affinity chromatography (Gold Biotechnology, Inc.). The b12 Fab ...
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bioRxiv - Microbiology 2022Quote: ... cells were induced with 50 μM Isopropyl β-d-1-thiogalactopyranoside (IPTG) (GoldBio). Expression was carried out at 8 °C and cells were collected at 20 hours after inoculation by centrifugation at 5000×g for 20 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with 200 μM auxin (Andole-3-Acetic Acid, Gold Biotechnology) and dTAG7 were added ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were washed in PBS 0.5% BSA (0.5% BSA, Goldbio 9048-46-8) in a round bottom 96-well plate (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were probed with the primary antibodies listed in Supplemental Table 1 overnight in 5% bovine serum albumin (Gold Biotechnology, Cat.# A-420-100) in TBST ...
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bioRxiv - Biophysics 2020Quote: ... Cells were grown in LB media supplemented with 0.2 mg/l of ampicillin (Goldbio) at 37 °C until OD600 of 1.0 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated with D-luciferin (Gold Biotechnology St. Louis, MO; LUCNA-1g) at 37°C and 5% CO2 for one hour in 24 well plate (200 μL/well) ...
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bioRxiv - Cancer Biology 2022Quote: ... All stable cell lines were selected using 2μg/ml of puromycin (Goldbio, P-600). For OVA overexpression ...
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bioRxiv - Microbiology 2021Quote: ... the cell lines were treated with two-fold doubling dilutions of nourseothricin (Gold Biotechnology), S-D ...
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bioRxiv - Biochemistry 2023Quote: ... the cells were induced with 0.4 mM isopropyl β-D-1-thiogalactopyranoside (IPTG, GoldBio). Post induction ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with 8 ug/ml of blasticidin (Gold Biotechnology, B-800-100) for 48 h to select for cells that had integrated the Xlone-mScarlet-Myo10 plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... coli (ZymoResearch T3001) cells and plated on selection media (Ampicillin, Goldbio 69-52-3). Sanger sequencing confirmed plasmids that contained the correct protease insert ...
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bioRxiv - Molecular Biology 2023Quote: ... coli (ZymoResearch T3001) cells and plated on selection media (Ampicillin, Goldbio 69-52-3). A green-white screen46 was used to inoculate assembled colonies in selection liquid media ...
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bioRxiv - Biophysics 2022Quote: ... C43 (DE3) cells and overexpressed in 1L minimal media A supplemented with ampicillin (Gold Biotechnology) as previously described ...
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bioRxiv - Biochemistry 2022Quote: ... and the cell pellet was resuspended in 0.5mg ml-1 luciferin (GoldBio; cat. no. LUCNA) solution prepared in 1X HBSS buffer (Gibco ...
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bioRxiv - Biochemistry 2019Quote: ... Media was aspirated and cells were resuspended in luciferin sodium solution (0.5mg/ml) (Gold Biotech) prepared in 1X Hanks Balanced Salt Solution (HBSS/Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... Each CRISPR/Cas9 plasmid construct was independently transformed into DH5-α Chemically Competent Cells (GoldBio) with chloramphenicol (25 µg/ml ...
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bioRxiv - Biophysics 2023Quote: ... The bacteria cells were lysed in buffer containing 50 mM HEPES pH 7.4 (#H400, Goldbio), 1.5 M NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... the total number of target cells was measured by adding D-luciferin potassium salt (Goldbio) to a final concentration of 0.5 mg/ml and reading the bioluminescence with the Infinite F Plex microplate reader (Tecan) ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then incubated on ice with serum free media containing 10 nM EGF (Gold Biotechnology) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... Expression of the provirus was induced by treating cells with doxycycline (GoldBio, St. Louis, MO, USA) at various concentrations ...
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bioRxiv - Biochemistry 2022Quote: ... Stable cells were selected and maintained with 2,500 µg/ml G-418 (Gold Biotechnology, G-418-25) in the medium with the exception of the 293T-based CAPN5-3×FLAG stable cell line that was selected and maintained with 200 µg/ml hygromycin (Gold Biotechnology ...
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bioRxiv - Bioengineering 2022Quote: ... Bacterial cells were grown in LB broth Miller (Difco) containing chloramphenicol (25 μg/mL) (Gold Biotechnology, USA) at 37 °C to OD600 = 1.7 ...
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bioRxiv - Biochemistry 2021Quote: Cell lysates of Arpin wild-type and mutants C204S and W195D were incubated with glutathioneagarose resin (GoldBio) for 2 h at 4°C (rocking) ...
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bioRxiv - Plant Biology 2022Quote: ... ~0.5cm root tips were harvested from 1000-3000 roots and placed into a 35mm petri dish containing a 70 μm cell strainer and 4.5mL enzyme solution (1.5% [w/v] cellulase [ONOZUKA R-10, GoldBio] ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were washed once with 1× PBS and 75 μL of 2 mM D-luciferin (LUCK-1G, Goldbio) in Leibovitz’s L-15 medium (21083-027 ...