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Citations for Gold Biotechnology :
1 - 50 of 233 citations for Recombinant HIV 1 gp120 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
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bioRxiv - Immunology 2023Quote: ... All recombinant human cytokines were purchased from GoldBio (Massachusetts, USA). Following stimulation ...
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bioRxiv - Cancer Biology 2020Quote: Human recombinant IL-1α (GoldBio, St. Louis, MO; 1110-01A-100), IL-1β (GoldBio ...
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bioRxiv - Cancer Biology 2019Quote: Human recombinant IL-1α (GoldBio, St Louis MO; 1110-01A-100) and IL-1β (GoldBio ...
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bioRxiv - Biochemistry 2020Quote: ... The hexahistidine-tagged proteins were affinity purified from soluble lysate using nickel agarose beads (Gold Biotechnology). Bound protein was washed with 50 mL lysis buffer before elution using 5 mL of elution buffer containing 25 mM Tris HCl pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... The hexahistidine-tagged DXR protein was affinity purified from soluble lysate via nickel agarose beads (Gold Biotechnology). Bound protein was eluted in 300 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... The His6-tagged protein variants present in the supernatant were purified using Ni2+-NTA affinity chromatography (Goldbio) and dialyzed overnight at 4 ⁰C against 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2019Quote: ... The hexahistidine-tagged DXR protein was affinity purified from soluble lysate via nickel agarose beads (Gold Biotechnology). Bound protein was eluted in 300 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... expression of recombinant protein was induced with 0.5 mM isopropyl β-D-thiogalactoside (GoldBio). For purification of PABPC1 and ePAB ...
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bioRxiv - Biochemistry 2020Quote: ... recombinants were selected on YPD agar plates containing 100 μg/ml nourseothricin (Gold Biotechnology), resulting in ySI42 ...
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bioRxiv - Immunology 2020Quote: ... Recombinant human interleukin-15 (IL-15) and Fibroblast Growth Factor-basic (bFGF) were purchased from GoldBio. Recombinant Human RANTES (CCL5 ...
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bioRxiv - Immunology 2020Quote: ... Clarified cell supernatant containing recombinant antibodies was passed over Protein A Agarose (Goldbio Cat# P-400-5), followed by extensive washing with PBS ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (GoldBio) was added to induce protein expression at 37 °C for 4 h ...
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bioRxiv - Cell Biology 2022Quote: ... and rifampicin (GoldBio #R-120-1, 100 μg mL-1). Bacterial treatments for actin dynamics analysis were conducted following previously described methods36 ...
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bioRxiv - Biophysics 2023Quote: ... 1 μg ml-1 pepstatin A (P-020-100, GoldBio), 100 μg ml−1 4-(2-Aminoethyl ...
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bioRxiv - Biochemistry 2024Quote: ... and induced with 1 mM Isopropyl b-d-1-thiogalactopyranoside (IPTG, Goldbio) overnight at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... 1 mM IPTG (Goldbio) was added and the cultures were set at 15 °C for 24 h (230 rpm) ...
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bioRxiv - Plant Biology 2021Quote: ... 1 mM PMSF (GoldBio), 1X Halt Protease Inhibitor Cocktail (Thermo Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM luciferin (GOLDBIO) was applied onto leaves and the images were captured using Lumazone imaging system equipped with 2048B CCD camera (Roper).
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bioRxiv - Biochemistry 2024Quote: ... 1 mM TCEP (GoldBio), 5% glycerol (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... 1 mM IPTG (GoldBio), 2 mg/ml L-arabinose (GoldBio) ...
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bioRxiv - Microbiology 2023Quote: ... CYE was supplemented with 10 µg mL-1 chloramphenicol for plasmid maintenance and legC4 expression was induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG; GoldBio) where indicated ...
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bioRxiv - Microbiology 2019Quote: ... pneumophila was induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG) (GoldBio).
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bioRxiv - Synthetic Biology 2021Quote: ... We used 1 mM of Isopropyl β-D-1-thiogalactopyranoside (IPTG, Gold Biotechnology) for the induction of FnCpf1.
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bioRxiv - Developmental Biology 2023Quote: ... 1 μg/mL leupeptin) containing 1× ProBlock™ Protease Inhibitor Cocktail-50 (GoldBio) and processed as previously described[17] ...
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bioRxiv - Synthetic Biology 2021Quote: ... cultures were induced with 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG, Gold Biotechnology) and then kept at 25 °C with shaking at 225 rpm for 24 h ...
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bioRxiv - Biochemistry 2022Quote: ... protein expression was induced with 1 mM IPTG (isopropyl β-D-1-thiogalactopyranoside; GoldBio) followed by expression for 17 hr at 25°C ...
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bioRxiv - Molecular Biology 2021Quote: ... (1 mg/ml, Gold Biotechnology). SC-Leu+MPA media is minimal media of SC-Leu supplemented with mycophenolic acid (20 ug/ml ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM AEBSF (Gold Biotechnology), 1 mM Benzamidine (Sigma-Aldrich) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1 mM IPTG (GoldBio I2481C), 400 μM erythromycin ...
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bioRxiv - Plant Biology 2021Quote: ... and 1 mM DTT (GoldBio). Protein concentration was measured using the Bradford method (Bradford ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM X-Gluc (GoldBio), and 0.01% Silwet L-77) ...
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bioRxiv - Biophysics 2023Quote: ... 1% of DDM detergent (GoldBio) was added to the lysed cell and the cells was incubated for 1 h at 4°C to extract the membrane protein ...
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bioRxiv - Bioengineering 2023Quote: ... 1 mM IPTG (GoldBio, I2481C), 1mM m-toluic acid (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mM dithiothreitol (GoldBio, DTT25), 5 mM NTP Set (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM IPTG (I2481, GoldBio) was added and the cultures were shifted to 18°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... 1 mM IPTG (Gold Biotechnology) was added to induce the protein expression ...
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bioRxiv - Biochemistry 2021Quote: ... Lipin 1 and lipin 1 ΔM-Lip proteins were purified using Ni-NTA resin (GoldBio). Eluted protein was applied to Streptactin-XT resin equilibrated with equilibration buffer (100 mM Tris ...
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bioRxiv - Microbiology 2019Quote: ... 100 µg mL-1 ampicillin (GoldBio) and 25 µg mL-1 chloramphenicol (GoldBio)] ...
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bioRxiv - Microbiology 2021Quote: ... and 1 mM D-Luciferin (GoldBio) in Tris-HCL ...
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bioRxiv - Cell Biology 2021Quote: ... Puromycine (1 μg/ml,2h, GoldBio), α-Amanitin (5 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... 100 μg ml−1 ampicillin (Goldbio), 200 μg ml−1 hygromycin (Thermofisher) ...
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bioRxiv - Microbiology 2021Quote: ... 5 μg ml−1 gentamicin (Goldbio) and 35 μg ml−1 chloramphenicol (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... and 1 mM D-Luciferin (GoldBio) in Tris-HCL ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 mM DTT (Gold Biotech, DTT50), 1 mM PMSF (RPI ...
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bioRxiv - Immunology 2021Quote: ... and 1 mM D-Luciferin (GoldBio) in Tris-HCL ...
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bioRxiv - Immunology 2020Quote: ... and 1 mM D-Luciferin (GoldBio) in Tris-HCl on a BertholdTech TriStar2S luminometer.
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bioRxiv - Microbiology 2021Quote: ... 50 μg ml−1 kanamycin (Goldbio), 100 μg ml−1 ampicillin (Goldbio) ...
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bioRxiv - Immunology 2022Quote: ... and 1 mM D-Luciferin (GoldBio) in Tris-HCL) ...
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bioRxiv - Microbiology 2023Quote: ... 25 µg mL-1 chloramphenicol (GoldBio), or 100 µg mL-1 ampicillin (GoldBio)] ...