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Citations for Gold Biotechnology :
1 - 50 of 245 citations for Mumps virus lysate Isolate 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... GST lysates were incubated with glutathione agarose resin (GoldBio; 50 μL resin per 0.5-1.5 mL of lysate) for 30 min at 4°C and then washed 6x (3x quick washes ...
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bioRxiv - Biophysics 2024Quote: ... Cleared lysate was loaded onto lysis buffer-equilibrated Ni-NTA resin (GoldBio, 5 mL resin per 50 mL lysate) and rocked gently for 1 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... GST lysates were incubated with glutathione agarose resin (GoldBio) for 30 min at 4°C and then washed 6x (3x quick washes ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... Cell lysate was clarified by centrifugation and glutathione agarose resin (GoldBio) was added to affinity purify the GST-GGA3 ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was then loaded onto Co2+-charged TALON resin (GoldBio), equilibrated previously with the wash buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysates were incubated with equilibrated Glutathione agarose (Gold Biotechnology) for 2 h at 4 °C and the beads washed in a gravity column using 50 ml lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: Ashbya spores were plated onto Ashbya Full Media (AFM) for wild-isolates or AFM with Nourseothricin Sulfate (GoldBio, N-500-100, St. Louis. MO, USA) for wild-isolates with tagged Whi3 ...
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bioRxiv - Biochemistry 2020Quote: ... Cleared lysate was added to 200 μL of Nickel beads (Gold Biotech) in a small drip column (BioRad) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cleared lysates were incubated with 4 mL glutathione agarose resin slurry (GoldBio) for 1 h at 4° C to capture GST-KIX ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysates were fractionated on a Ni2+-NTA agarose column (Gold Biotechnology). RPA was eluted using cell resuspension buffer containing 400 mM imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... Lysate was run over a Co2+ agarose bead (GoldBio, H-310-25) column at 1 mL/min ...
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bioRxiv - Biochemistry 2022Quote: ... the cleared cell lysate was loaded onto a gravity column with 1 mL bed volume of Glutathione agarose resin (Gold Biotechnology, #G-250-5). The resin was extensively washed ...
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bioRxiv - Microbiology 2020Quote: ... clarified cell lysates were purified by protein G affinity chromatography (Gold Biotechnology, Inc.). The b12 Fab ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were applied to GST agarose column (Glutathione agarose resin, GoldBio Cat# G-250-5) and washed 3 times in wash buffer 1x TBS + 1mM DTT) ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were clarified using centrifugation and proteins were purified via nickel agarose beads (Gold Biotechnology), eluted with 300mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... The hexahistidine-tagged proteins were affinity purified from soluble lysate using nickel agarose beads (Gold Biotechnology). Bound protein was washed with 50 mL lysis buffer before elution using 5 mL of elution buffer containing 25 mM Tris HCl pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... GST-KatG was purified from the supernatant by incubating lysate overnight with glutathione agarose resin (Goldbio), washed with 300mL 1X PBS ...
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bioRxiv - Microbiology 2022Quote: ... The virus-serum mix was subsequently added to Vero cells for 48 h before 50 nmoles of D-Luciferin (GoldBio, St Lous, MO, USA) was added to measure luminescence ...
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bioRxiv - Microbiology 2020Quote: ... The hexahistidine-tagged DXR protein was affinity purified from soluble lysate via nickel agarose beads (Gold Biotechnology). Bound protein was eluted in 300 mM imidazole ...
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bioRxiv - Microbiology 2019Quote: ... Lysates were clarified using centrifugation and proteins were purified via nickel agarose beads (Gold Biotechnology, Olivette, MO), eluted with 300mM imidazole ...
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bioRxiv - Microbiology 2019Quote: ... The hexahistidine-tagged DXR protein was affinity purified from soluble lysate via nickel agarose beads (Gold Biotechnology). Bound protein was eluted in 300 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: Cell lysates of Arpin wild-type and mutants C204S and W195D were incubated with glutathioneagarose resin (GoldBio) for 2 h at 4°C (rocking) ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were clarified via centrifugation and applied to 4 mL of pre-equilibrated Ni+-NTA resin (GoldBio) in a gravity flow column ...
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bioRxiv - Biochemistry 2020Quote: ... lysate was cleared at 24,000 xg for one hours and the supernatant incubated with glutathione agarose resin (Goldbio) for 2 hours ...
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bioRxiv - Biochemistry 2022Quote: ... FN3-AFF sensors were purified from the soluble fractions of cell lysates using nickel nitriloacetate resin (Gold Biotechnology, St. Louis, MO) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (GoldBio) was added to induce protein expression at 37 °C for 4 h ...
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bioRxiv - Cell Biology 2022Quote: ... and rifampicin (GoldBio #R-120-1, 100 μg mL-1). Bacterial treatments for actin dynamics analysis were conducted following previously described methods36 ...
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bioRxiv - Biophysics 2023Quote: ... 1 μg ml-1 pepstatin A (P-020-100, GoldBio), 100 μg ml−1 4-(2-Aminoethyl ...
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bioRxiv - Biochemistry 2024Quote: ... and induced with 1 mM Isopropyl b-d-1-thiogalactopyranoside (IPTG, Goldbio) overnight at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... 1 mM IPTG (Goldbio) was added and the cultures were set at 15 °C for 24 h (230 rpm) ...
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bioRxiv - Plant Biology 2021Quote: ... 1 mM PMSF (GoldBio), 1X Halt Protease Inhibitor Cocktail (Thermo Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM luciferin (GOLDBIO) was applied onto leaves and the images were captured using Lumazone imaging system equipped with 2048B CCD camera (Roper).
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bioRxiv - Biochemistry 2024Quote: ... 1 mM TCEP (GoldBio), 5% glycerol (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... 1 mM IPTG (GoldBio), 2 mg/ml L-arabinose (GoldBio) ...
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bioRxiv - Microbiology 2023Quote: ... CYE was supplemented with 10 µg mL-1 chloramphenicol for plasmid maintenance and legC4 expression was induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG; GoldBio) where indicated ...
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bioRxiv - Microbiology 2019Quote: ... pneumophila was induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG) (GoldBio).
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bioRxiv - Synthetic Biology 2021Quote: ... We used 1 mM of Isopropyl β-D-1-thiogalactopyranoside (IPTG, Gold Biotechnology) for the induction of FnCpf1.
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bioRxiv - Developmental Biology 2023Quote: ... 1 μg/mL leupeptin) containing 1× ProBlock™ Protease Inhibitor Cocktail-50 (GoldBio) and processed as previously described[17] ...
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bioRxiv - Synthetic Biology 2021Quote: ... cultures were induced with 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG, Gold Biotechnology) and then kept at 25 °C with shaking at 225 rpm for 24 h ...
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bioRxiv - Biochemistry 2022Quote: ... protein expression was induced with 1 mM IPTG (isopropyl β-D-1-thiogalactopyranoside; GoldBio) followed by expression for 17 hr at 25°C ...
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bioRxiv - Molecular Biology 2021Quote: ... (1 mg/ml, Gold Biotechnology). SC-Leu+MPA media is minimal media of SC-Leu supplemented with mycophenolic acid (20 ug/ml ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM AEBSF (Gold Biotechnology), 1 mM Benzamidine (Sigma-Aldrich) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1 mM IPTG (GoldBio I2481C), 400 μM erythromycin ...
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bioRxiv - Plant Biology 2021Quote: ... and 1 mM DTT (GoldBio). Protein concentration was measured using the Bradford method (Bradford ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM X-Gluc (GoldBio), and 0.01% Silwet L-77) ...
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bioRxiv - Biophysics 2023Quote: ... 1% of DDM detergent (GoldBio) was added to the lysed cell and the cells was incubated for 1 h at 4°C to extract the membrane protein ...
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bioRxiv - Bioengineering 2023Quote: ... 1 mM IPTG (GoldBio, I2481C), 1mM m-toluic acid (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mM dithiothreitol (GoldBio, DTT25), 5 mM NTP Set (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM IPTG (I2481, GoldBio) was added and the cultures were shifted to 18°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... 1 mM IPTG (Gold Biotechnology) was added to induce the protein expression ...