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Citations for Gold Biotechnology :
1 - 50 of 106 citations for Mouse Anti Hepatitis C Virus Core Protein Antibody 1868 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and antibody was purified using protein A agarose chromatography (Goldbio). Sequences were obtained from PCR reaction products and annotated using the ImMunoGeneTics (IMGT)/V- QUEST database (http://www.imgt.org/IMGT_vquest/)51,52 ...
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bioRxiv - Molecular Biology 2021Quote: Purification of the mouse lectin-mIgG2a fusion proteins was performed using Protein A agarose resin (Gold Biotechnology, P-400-5). The protein A beads were pelleted at 150g for 5 min and washed once with 1x binding buffer (0.02 M Sodium Phosphate ...
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bioRxiv - Biophysics 2023Quote: ... The temperature was then set to 30 °C and protein expression was induced by adding 0.2 % arabinose (Goldbio). Cells were grown for additional 16 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Bacteria grew at 37°C and protein induction occurred at 20°C for overnight upon addition of 0.1mM isopropyl D-1-thigalactopyranoside (IPTG; Goldbio, I2481C) to the bacterial culture at mid-log phase (OD600nm=0.6) ...
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bioRxiv - Immunology 2022Quote: ... 4.5 mg E1D1 antibody was incubated with 1 mL Protein A resin (GoldBio, P-400) with rotation at room temperature for 30 minutes and then washed thoroughly with PBS ...
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bioRxiv - Molecular Biology 2023Quote: To the soluble fraction from isolated cores (prepared as described above) 25mM TCEP (Gold Biotechnology, no. 51805-45-9) and 4% SDS (Carl Roth ...
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bioRxiv - Biophysics 2023Quote: ... the temperature was reduced to 20 °C and protein expression was induced with the addition of IPTG (GoldBio, St. Louis, MO, USA) to a final concentration of 1 mM ...
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bioRxiv - Immunology 2020Quote: ... Clarified cell supernatant containing recombinant antibodies was passed over Protein A Agarose (Goldbio Cat# P-400-5), followed by extensive washing with PBS ...
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bioRxiv - Microbiology 2020Quote: ... alongside a protein molecular weight marker (BLUEstain protein ladder, Gold Biotechnology). Gels were run at 100 V for 15 min and then 150 V for 40 min in NuPAGE MES SDS running buffer and the proteins were subsequently transferred onto PVDF or nitrocellulose membranes using iBlot transfer stacks (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 100 μg/mL carbenicillin (GoldBio C-103), 25 μg/mL chloramphenicol (GoldBio C-105 ...
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bioRxiv - Biochemistry 2022Quote: Chloramphenicol (Goldbio, cat. no. C-105-5)
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bioRxiv - Bioengineering 2023Quote: ... 100 µg/ml carbenicillin (GoldBio, C-103) and 25 µg/ml chloramphenicol (GoldBio ...
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bioRxiv - Immunology 2021Quote: ... RBD proteins were purified using protein A agarose resin (Goldbio CAT# P-400), followed by on-column cleavage with HRV3C protease (made in-house ...
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bioRxiv - Microbiology 2022Quote: ... The virus-serum mix was subsequently added to Vero cells for 48 h before 50 nmoles of D-Luciferin (GoldBio, St Lous, MO, USA) was added to measure luminescence ...
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bioRxiv - Biochemistry 2020Quote: ... and His SUMO tags were cleaved with Ulp1 protease overnight at 4°C in Buffer C (25mM Tris-HCl pH 8.5, 150mM KCl, 2mM TCEP (GoldBio), 50mM Imidazole ...
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bioRxiv - Microbiology 2020Quote: ... Secreted hACE2-Fc and mACE2-Fc proteins were purified by protein A chromatography (Goldbio).
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bioRxiv - Biochemistry 2023Quote: ... and 0.05% CHAPS (GoldBio, Cat# C-080-100). We pooled and concentrated peak fractions to 5-10μM before we aliquoted and flash froze with liquid nitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... after 10 μM coelenterazine 400a (#C-320, Gold Biotechnology) addition ...
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bioRxiv - Bioengineering 2023Quote: Carbenicillin disodium (Gold Biotechnology cat. no. C-103-5)
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bioRxiv - Biochemistry 2023Quote: ... Then 10 µM of coelenterazine 400a (GoldBio, #C-320) was added to the cells and BRET readings were recorded simultaneously at λemission 410 ± 40 nm (RLU ...
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bioRxiv - Biochemistry 2022Quote: ... 10μl of a 20μM coelenterazine 400A (GoldBio; cat. no. C-320) solution diluted in Krebs/HEPES was added 5min before the end of the stimulation period ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein expression was induced with IPTG (GoldBio, I2481C) at 16°C for 18 hours ...
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bioRxiv - Immunology 2021Quote: ... followed by purification with protein A agarose resin (GoldBio). Expi293F cells were cultured in Expi293 Expression Medium (Gibco ...
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bioRxiv - Biophysics 2020Quote: ... Protein expression was induced by adding 0.2 % arabinose (Goldbio) for 3 hr at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... and mAbs were purified with protein A agarose (GoldBio).
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bioRxiv - Immunology 2020Quote: ... and incubated overnight with Protein A Agarose Resin (GoldBio). The resin was collected in a chromatography column ...
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bioRxiv - Microbiology 2023Quote: ... and incubated overnight with Protein A Agarose Resin (GoldBio). The resin was collected in a chromatography column ...
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bioRxiv - Microbiology 2023Quote: ... and incubated overnight with Protein A Agarose Resin (GoldBio) at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... or Protein A Agarose Resin (Gold Biotechnology, P-400-5). Beads and antibody were incubated and rotated at room temperature for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Bulk IgGs were captured by protein A agarose resin (GoldBio) at 4°C rotating overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Heterologous protein expression was induced with 0.5 mM IPTG (GoldBio), and cultures were grown overnight at 19°C with gentle agitation.
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bioRxiv - Molecular Biology 2021Quote: ... Protein expression was induced by adding 0.5 mM IPTG (GoldBio) and grown overnight at 16°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein expression was induced by adding 1 mM IPTG (GoldBio) and grown at 37°C for 5 h with agitation.
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bioRxiv - Molecular Biology 2021Quote: ... Protein expression was induced by adding 0.5 mM IPTG (GoldBio) followed by growth overnight at 16°C ...
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bioRxiv - Immunology 2023Quote: WP and HIP were mixed with Protein A-agarose (GoldBio), Protein G-agarose (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... then stimulated with the coelenterazine 400A substrate (5 mM) (Gold Biotechnology #C-320-1) and read for 30 min on Mithras2 Plate Reader (Berthold ...
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bioRxiv - Microbiology 2021Quote: ... Amersham) and protein expression induced with 1 mM final IPTG (Goldbio) for 4 hr at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The supernatant was filtered and passed over Protein A resin (GoldBio), washed with 10 mM sodium phosphate pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was purified using glutathione agarose resin (Gold Biotechnology Inc.). The protein was subjected to TEV cleavage to remove the GST tag and then further purified using a Mono Q 5/50 GL anion exchange column (GE Healthcare Life Sciences) ...
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bioRxiv - Biochemistry 2022Quote: ... The digested protein was passed over a 2-mL Ni-HTC (GoldBio) gravity column to separate the tag-free NnlA ...
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bioRxiv - Immunology 2021Quote: ... The cleaved product was incubated with 1mL of Protein A resin (GoldBio) per 10mg of initial IgG and incubated at room temp for 1 hr to bind any uncleaved IgG and digested Fc ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
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bioRxiv - Microbiology 2020Quote: ... clarified cell lysates were purified by protein G affinity chromatography (Gold Biotechnology, Inc.). The b12 Fab ...
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bioRxiv - Immunology 2020Quote: ... The RBD was purified using protein A agarose resin (GoldBio, Cat# P-400) and cleaving the Fc domain using HRV3C protease (made in house ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant containing GST-Rev1 protein was incubated with glutathione agarose resin (Goldbio) for 2 hours ...
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bioRxiv - Physiology 2022Quote: ... N2 worms were egg-prepped onto solid NGM with 50 μg/ml carbenicillin (GoldBio, C-103-5) and HT115(DE3 ...
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bioRxiv - Biophysics 2024Quote: ... at 18 °C for 16 hours after induction with 0.2 mM isopropyl-β-D-thiogalactoside (Goldbio, I2481C100). The cells were resuspended and disrupted in lysis buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... Each mouse was intraperitoneally injected with 3-mg D-luciferin (Gold Biotechnology Inc.) in 200-μL PBS before observation ...
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bioRxiv - Molecular Biology 2021Quote: ... expression of recombinant protein was induced with 0.5 mM isopropyl β-D-thiogalactoside (GoldBio). For purification of PABPC1 and ePAB ...
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bioRxiv - Biochemistry 2020Quote: ... Induction of protein expression was induced by addition of 1 mM IPTG (Gold Biotech) and cells were grown overnight at 18°C ...