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Citations for Gold Biotechnology :
351 - 400 of 404 citations for Methylphosphonic Acid 13C 99%; Methyl D3 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... then the cleared bacterial lysate was passed through a 0.22 μm PES filter (Genessee Scientific) and then applied to a 5 mL packed Ni-NTA agarose beads (Goldbio) equilibrated with Lysis buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 120 μl of 20 mg/ml 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-gal, GoldBio) were overlaid on LB agar plates ...
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bioRxiv - Cancer Biology 2023Quote: ... Mice were intraperitonially injected with 200 μL D-Luciferin (15 mg/mL; Gold Biotechnology, Inc., St. Luis, MO, USA), and Luminescence was taken under anesthesia using IVIS ...
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bioRxiv - Biophysics 2023Quote: ... The suspended cells were broken by an Emulsiflex C3 high-pressure homogenizer (Avestin Inc.) in the presence of 0.5 mg/ml lysozyme (Goldbio) and 1 mM phenylmethanesulfonyl fluoride (PMSF ...
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bioRxiv - Plant Biology 2023Quote: ... containing 30 µL of 300 mg/mL timentin (final concentration of 300 mg/L) (T-104-25, Goldbio, USA), followed by homogenization with a T18 digital ULTRA TURRAX homogenizer (IKA ...
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bioRxiv - Biochemistry 2023Quote: ... the clarified supernatant containing RPA4AZP was applied to a 3 mL pre-equilibrated Ni2+-NTA (Gold Biotechnology Inc., USA) gravity column ...
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bioRxiv - Immunology 2024Quote: ... Samples were treated with 1µl D-luciferin (30µg/mL) per 100µl sample volume (Gold Biotechnology, St. Louis, MO, USA) prior to imaging.
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bioRxiv - Microbiology 2019Quote: ... Yale University] were maintained in Luria-Bertani (LB) medium supplemented with antibiotics as appropriate for plasmid selection [50 µg mL-1 kanamycin (GoldBio), 100 µg mL-1 ampicillin (GoldBio ...
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bioRxiv - Genomics 2020Quote: ... Standard 96-well plate medium was used for all camera runs (1X Vogel’s Salts, 0.03% glucose, 0.05% arginine, 1.5% agar, 50 ng/ml biotin, and 25 μM luciferin from GoldBio # 115144-35-9). Liquid cultures were started from fungal plugs as described (Chen et al. ...
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bioRxiv - Biophysics 2022Quote: ... A transformant colony was used to inoculate Luria–Bertani (LB) media (Fisher Bioreagents) containing 0.1 mg/mL ampicillin (Gold Biotechnology), which was grown overnight (∼15 h ...
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bioRxiv - Microbiology 2020Quote: ... Mice infected with Fluc-carrying viruses received in vivo grade D-Luciferin (15 mg/mL in sterile endotoxin-free PBS, GoldBio) (r.o. ...
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bioRxiv - Biochemistry 2021Quote: ... cells were harvested and resuspended in assay buffer containing D-luciferin (0.5 mg ml−1, GoldBio, Cat. no. LUCNA-1G) in 1X HBSS ...
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bioRxiv - Cell Biology 2019Quote: ... was measured at the indicated intervals by replacing 10% of media with MuSC media supplemented with 1.5mg/ml D-Luciferin (Gold Biotechnology) and recorded using an IVIS Spectrum (Perkin Elmer).
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bioRxiv - Microbiology 2020Quote: ... The protein was eluted with 5 mL of elution buffer (20 mM TEA, 50 mM KCl, 500 mM imidazole (GoldBio), pH 7) ...
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bioRxiv - Microbiology 2020Quote: ... The column was washed with 40 mL of wash buffer (20 mM TEA, 50 mM KCl, 50 mM imidazole (GoldBio), pH 7) ...
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bioRxiv - Immunology 2020Quote: ... Cells were plated at equal density and luminescence signals were quantified in the presence of 150 µg/ml D-luciferin (GoldBio) by imaging using cooled CCD optical macroscopic imaging system (IVIS Lumina Series III ...
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bioRxiv - Microbiology 2022Quote: For the lacP1 measurements strains containing the reporter plasmid were grown on LB agar plates with 400 μg/ml Carbenicillin (GoldBio) at 37°C to obtain single colonies ...
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bioRxiv - Cell Biology 2022Quote: ... animals were grown on 6 cm NGM plates that were supplemented with 250 μl of 5 mg/ml of hygromycin B (GoldBio) in M9.
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then incubated in X-gal staining solution (X-gal rinse buffer plus 1 mg/mL X-gal (GoldBio X4281C ...
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bioRxiv - Microbiology 2023Quote: ... New England Biolabs] were grown at 37 °C in Luria-Bertani (LB) medium supplemented with antibiotics as appropriate for plasmid selection [50 µg mL-1 kanamycin (GoldBio), 25 µg mL-1 chloramphenicol (GoldBio) ...
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bioRxiv - Cancer Biology 2024Quote: ... the mice were injected intraperitoneally with 10 μl/g of 15 mg/mL D-luciferin potassium salt solution (Gold Biotechnology) and imaged using the Xenogen IVIS 200 In Vivo Imaging System (PerkinElmer) ...
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bioRxiv - Biochemistry 2020Quote: ... Single colonies were isolated from YPD (1% yeast extract, 2% peptone, 2% glucose) agar plates containing 400 μg/ml hygromycin (Gold Biotechnology), and integration was verified by PCR ...
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bioRxiv - Plant Biology 2020Quote: ... Chemiluminescence was detected using the NightSHADE LB 985 system (Berthold) in the presence of 20 mg mL−1 potassium luciferin (Gold Biotech). The LUC/REN ratio was calculated to infer effectiveness of miR775 targeting.
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bioRxiv - Biophysics 2020Quote: ... 1% of primary culture was inoculated in 750 mL of LB media and induced with 0.3 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Gold Biotechnology) at OD600 ~ 0.6 and further incubated at 16 °C for 14–16 h with constant shaking at 200 rpm ...
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bioRxiv - Cancer Biology 2021Quote: ... Live-cell luciferase readings were performed for 3 consecutive days by adding 150 μg/mL luciferine (GOLDBIO, Catalog No. LUCK-500) and measuring luminescence in a Tecan Infinite F500 reader (Tecan).
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bioRxiv - Cancer Biology 2022Quote: ... recipe of FACS buffer was RPMI-1640 + 5% FBS + 2% BSA + 50 µg/ml DNase-I (Gold Biotechnology, D-301-500). For all other primary cell staining ...
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bioRxiv - Cancer Biology 2022Quote: ... Antigen retrieval was performed by incubating the sections with Proteinase K (20 μg/ml in Tris-EDTA buffer) (Goldbio, St.Louis, MO) for 20 minutes at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Injected postnatal day one old mice were returned to the dam and after one week injected intraperitoneally with 150mg/kg firefly D-luciferin (sterile filtered 15mg/ml in phosphate buffered saline, Gold Biotechnology) (48 ...
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bioRxiv - Cancer Biology 2023Quote: ... Luc2 and NLuc expression was confirmed by exposing cells seeded in the wells of a flat-bottom white 96-well plate to 1 volume of 15 mg/mL D-luciferin (#LUCNA-1G, Goldbio, USA) or 1 volume of 0.5 mg/mL Nano-Glo In Vivo Substrate ...
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bioRxiv - Microbiology 2023Quote: ... CYE was supplemented with 10 µg mL-1 chloramphenicol for plasmid maintenance and legC4 expression was induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG; GoldBio) where indicated ...
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bioRxiv - Microbiology 2023Quote: ... after completion of virus infection virus containing media was replaced with media containing 5 µg/ml MTT reagent (Thiazolyl blue tetrazolium bromide, cat no.298-93-1, Gold biotechnology). Incubate at 37°C for 3-4 hr and then remove MTT containing media and add 100 µl of DMSO to each well to dissolve the formazan crystals formed ...
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bioRxiv - Biochemistry 2024Quote: ... media in the wells was replaced with the assay buffer (20mM HEPES pH 7.4, 1X Hank’s Balanced Salt Solution/ HBSS and 0.5mg/mL D-luciferin (GoldBio, Cat. no: LUCNA-1G). Luminescence was recorded immediately in a microplate reader (BMG Labtech) ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in assay buffer (20mM HEPES pH 7.4, 1X Hank’s Balanced Salt Solution/ HBSS and 0.5mg/mL D-luciferin (GoldBio, Cat. no: LUCNA-1G) and seeded in 96-well plates at a density of 100,000 cells/well ...
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bioRxiv - Cell Biology 2024Quote: ... and cultured axenically at 21°C in HL5 media (Froquet et al., 2009) supplemented with 300 µg/mL Streptomycin Sulfate (Gold Biotechnology) on 10 cm plates ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then serially diluted in sterile water and 5 µL of each dilution was plated to-trp Gal/Raf plates supplemented with 1mg/ml 5-FOA (Gold Biotechnology). After 4 days at 30 degrees ...
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bioRxiv - Microbiology 2020Quote: ... prepared in PBS and then incubated overnight at 37°C (without carbon dioxide) with freshly prepared staining solution (1mg/ml of X-gal (GoldBio Technology, USA) in 40 mM citric acid/sodium phosphate ...
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bioRxiv - Synthetic Biology 2021Quote: ... and to this 0.85 mL of liquid (70 °C) top agar (2XYT media + 0.6% agar) supplemented with 2% Bluo-Gal (GoldBio, CAS #97753-82-7) was added and mixed by pipetting up and down once ...
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bioRxiv - Microbiology 2020Quote: ... Strains were patched onto medium containing 50 g/ml X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside; Gold Biotechnology), 1 mM IPTG ...
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bioRxiv - Cell Biology 2022Quote: ... and screening for loss of b-glucuronidase activity via plating on YT plates containing 50 μg/ml X-Gluc (cyclohexlammonium salt, Gold Biotechnologies, G1281C1). Strains were confirmed by PCR amplification and DNA sequencing of the region of interest.
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bioRxiv - Biochemistry 2022Quote: ... cells were detached from the plates, resuspended in assay buffer (1XHBSS, 20 mM HEPES, pH7.4) containing D-luciferin (0.5 mg ml-1, GoldBio, Cat. no. LUCNA-1G) and seeded into 96 well white plates (Corning ...
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bioRxiv - Microbiology 2020Quote: ... ATP-free or ATP-containing (150 μM ATP) substrate solutions were prepared containing 150 μg/mL D-Luciferin (Goldbio, St. Louis, MO), 100 mM Tris pH 8 ...
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bioRxiv - Microbiology 2023Quote: ... were transformed and plated on LB Agar (Fisher BioReagents Cat#: BP1425-500) containing 50 µg/mL carbenicillin (GoldBio Cat#: C-103-5) and incubated overnight at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... resuspended in the assay buffer (20mM HEPES pH 7.4 and 1X HBSS) containing 0.5mg ml-1 of D-luciferin (GoldBio, Cat. no: LUCNA-1G) and seeded in 96-well white plates (SPL Life Sciences ...
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bioRxiv - Systems Biology 2023Quote: ... The G418-resistance cassettes were transformed into FY4 (MATa) and selected on YPD + G418 (400 µg/mL; Gold Biotechnology #G-418-5) plates ...
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bioRxiv - Systems Biology 2023Quote: ... The NAT-resistance cassettes were transformed into FY5 (MATα) and selected on YPD + NAT (200 µg/mL; Gold Biotechnology #N-500-1) plates ...
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bioRxiv - Molecular Biology 2023Quote: ... Single colonies from the selection plates were grown in selective LB media containing 50 μg/mL ampicillin (Goldbio Cat #: A-301-55) overnight in a 37 °C shaking incubator (180 rpm) ...
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bioRxiv - Cancer Biology 2020Quote: The cytokine used for Ba/F3 culturing was 10 ng/mL murine IL-3 (Cat.#31310-03-10; Gold Biotechnology, St Louis, US).
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bioRxiv - Genomics 2021Quote: ... and then stained with the indicated antibody in FACS buffer (PBS with 1% FBS, 10 U/ml DNAse I, GoldBio, St. Louis MO) for 30 minutes on ice with gentle agitation ...
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bioRxiv - Biochemistry 2022Quote: ... in the medium with the exception of the 293T-based CAPN5-3×FLAG stable cell line that was selected and maintained with 200 µg/ml hygromycin (Gold Biotechnology, H-270-1).
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bioRxiv - Biochemistry 2022Quote: ... the cleared cell lysate was loaded onto a gravity column with 1 mL bed volume of Glutathione agarose resin (Gold Biotechnology, #G-250-5). The resin was extensively washed ...