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Citations for Gold Biotechnology :
301 - 350 of 472 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... into a centrifuge tube and then lysed with lysis buffer (50mM Tris, pH 7.5, 150mM NaCl, and 2 mM Dodecyl-B-D-maltoside (DDM) (GoldBio, catalog #: DDM5) supplemented with complete protease inhibitor cocktail (Roche #04693116001) ...
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bioRxiv - Biochemistry 2024Quote: ... media in the wells was replaced with the assay buffer (20mM HEPES pH 7.4, 1X Hank’s Balanced Salt Solution/ HBSS and 0.5mg/mL D-luciferin (GoldBio, Cat. no: LUCNA-1G). Luminescence was recorded immediately in a microplate reader (BMG Labtech) ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in assay buffer (20mM HEPES pH 7.4, 1X Hank’s Balanced Salt Solution/ HBSS and 0.5mg/mL D-luciferin (GoldBio, Cat. no: LUCNA-1G) and seeded in 96-well plates at a density of 100,000 cells/well ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor volume was monitored through bioluminescence using IVIS Spectrum apparatus (Caliper Life Sciences) after i.p injection of D-luciferin 150mg/Kg (GOLDBIO #LUCK-1G). Mice were sacrificed when manifested more than 20% of body weight loss or neurological symptoms ...
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bioRxiv - Microbiology 2020Quote: ... clarified cell lysates were purified by protein G affinity chromatography (Gold Biotechnology, Inc.). The b12 Fab ...
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bioRxiv - Immunology 2020Quote: ... The RBD was purified using protein A agarose resin (GoldBio, Cat# P-400) and cleaving the Fc domain using HRV3C protease (made in house ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant containing GST-Rev1 protein was incubated with glutathione agarose resin (Goldbio) for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... Strains were patched onto medium containing 50 g/ml X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside; Gold Biotechnology), 1 mM IPTG ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed with lysis buffer (50 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM n-Dodecyl-B-D-maltoside (DDM) (GoldBio, catalog #: DDM-5)) supplemented with protease inhibitor cocktail (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... ATP-free or ATP-containing (150 μM ATP) substrate solutions were prepared containing 150 μg/mL D-Luciferin (Goldbio, St. Louis, MO), 100 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2020Quote: ... the culture was expanded 1:100 up to 2L cultures in Luria broth and induced at A600 ∼0.60 with 0.5 mM isopropyl β-D-thiogalactopyranoside (GoldBio, St. Louis MO) for 24 h at 16°C ...
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bioRxiv - Cell Biology 2021Quote: NSG mice transplanted with GAPDHLuciferase/wt and INSGFP/wt double reporter hESCs lines were i.p injected with 150mg/kg body weight of D-luciferin potassium salt (Gold Biotechnology, luck-2G) in PBS 15 minutes before imaging on a IVIS spectrum optical imaging system (PerkinElmer) ...
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bioRxiv - Bioengineering 2023Quote: ... Images were acquired 10 min after intraperitoneal injection of 3 mg d-Luciferin Potassium Salt (Gold Biotechnology, Inc., St Louis MO, USA) in 200 μL 0.1 M phosphate buffered saline ...
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bioRxiv - Cell Biology 2023Quote: Cells were harvested and lysed with lysis buffer (50 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM n-Dodecyl-β-D-maltoside (DDM) (GoldBio, catalog #: DDM5)) supplemented with complete protease inhibitor cocktail (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... Spectral bioluminescent images of live cells growing in vitro were acquired 2 minutes after adding 150 ug/ml of D-Luciferin (Goldbio, Saint Louis, MO). Sequential images were acquired through optical filters ranging from 520 to 660nm at 20nm intervals ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor progression was evaluated by luminescence emission on a Xenogen IVIS spectrum after intraperitoneal injection of D-luciferin (Gold Biotechnology, St. Louis, MO) injection according to the manufacturer’s directions ...
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bioRxiv - Bioengineering 2024Quote: ... Mice were imaged on the IVIS every 3-4 days after being sedated with Isoflurane and intraperitoneal injections of ∼120 mg/kg D-luciferin (GoldBio, St. Louis, MI). Mice were injected with 100,000 IU of human IL-2 (National Cancer Institute ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted by adding lysis buffer containing 35 mM reduced glutathione (Gold Biotechnology). The elute fractions were run on a 4-12% NuPAGE Bis-Tris protein gel (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were co-cultured overnight and luminescence was measured the next morning after addition of 150 μg/ml D-luciferin (Gold Biotechnology Cat# LUCNA-2G) and subsequent incubation for 5 mins at 37°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 5-bromo-4-chloro-3-indolyl-β-D-galactoside (X- GAL) (X4281C10) for blue-white screening were purchased from Gold Biotechnology (Olivette, Missouri). Lysozyme (L6876-10G) ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were clarified using centrifugation and proteins were purified via nickel agarose beads (Gold Biotechnology), eluted with 300mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... along with 2.5 μl of a pre-stained protein ladder (#P008; GoldBio, St. Louis, MO), and separated on an SDS-polyacrylamide gel ...
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bioRxiv - Immunology 2020Quote: Supernatant containing RBD-Fc was purified over protein A agarose resin (Goldbio, Cat# P-400), cleaved with HRV3C protease (made in house ...
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bioRxiv - Immunology 2021Quote: ... All GST fusion proteins were purified using Glutathione Agarose 4B (Gold Biotechnology, #G-250-50), and eluted with HEPES buffered saline (HBS ...
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bioRxiv - Biochemistry 2020Quote: ... The hexahistidine-tagged proteins were affinity purified from soluble lysate using nickel agarose beads (Gold Biotechnology). Bound protein was washed with 50 mL lysis buffer before elution using 5 mL of elution buffer containing 25 mM Tris HCl pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... the GltPh proteins were reduced with 500 µM tris(2-carboxy-ethyl) phosphine (TCEP, Gold Biotechnology) for 30 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The hexahistidine-tagged DXR protein was affinity purified from soluble lysate via nickel agarose beads (Gold Biotechnology). Bound protein was eluted in 300 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... The His6-tagged protein variants present in the supernatant were purified using Ni2+-NTA affinity chromatography (Goldbio) and dialyzed overnight at 4 ⁰C against 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2019Quote: ... Lysates were clarified using centrifugation and proteins were purified via nickel agarose beads (Gold Biotechnology, Olivette, MO), eluted with 300mM imidazole ...
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bioRxiv - Immunology 2020Quote: ... Clarified cell supernatant containing recombinant antibodies was passed over Protein A Agarose (Goldbio Cat# P-400-5), followed by extensive washing with PBS ...
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bioRxiv - Microbiology 2019Quote: ... The hexahistidine-tagged DXR protein was affinity purified from soluble lysate via nickel agarose beads (Gold Biotechnology). Bound protein was eluted in 300 mM imidazole ...
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bioRxiv - Cancer Biology 2020Quote: ... Mice were shaved front and back and Nair was applied to remove remainder of the hair before being IP injected with D-luciferin (Goldbio LUCK-1G, 150mg/kg in 150 mL). Mice are placed in a supine position on the light-tight chamber of the CCD camera imaging unit ...
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bioRxiv - Cell Biology 2022Quote: ... and rifampicin (GoldBio #R-120-1, 100 μg mL-1). Bacterial treatments for actin dynamics analysis were conducted following previously described methods36 ...
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bioRxiv - Biophysics 2023Quote: ... 1 μg ml-1 pepstatin A (P-020-100, GoldBio), 100 μg ml−1 4-(2-Aminoethyl ...
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bioRxiv - Biophysics 2023Quote: ... The temperature was then set to 30 °C and protein expression was induced by adding 0.2 % arabinose (Goldbio). Cells were grown for additional 16 hours ...
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bioRxiv - Biochemistry 2023Quote: ... Culture growth at 37 °C was monitored by measuring absorbance at 600 nm (A600) and protein expression was induced at A600 = ∼0.7 with 0.5 mM IPTG (GoldBio). The culture was grown at 18 °C for ∼15 hr ...
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bioRxiv - Bioengineering 2022Quote: ... 1 mM IPTG (Goldbio) was added and the cultures were set at 15 °C for 24 h (230 rpm) ...
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bioRxiv - Plant Biology 2021Quote: ... 1 mM PMSF (GoldBio), 1X Halt Protease Inhibitor Cocktail (Thermo Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM luciferin (GOLDBIO) was applied onto leaves and the images were captured using Lumazone imaging system equipped with 2048B CCD camera (Roper).
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bioRxiv - Bioengineering 2024Quote: ... 1 mM IPTG (GoldBio), 2 mg/ml L-arabinose (GoldBio) ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM TCEP (GoldBio), 5% glycerol (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... The protein was eluted with 5 mL of elution buffer (20 mM TEA, 50 mM KCl, 500 mM imidazole (GoldBio), pH 7) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were harvested by centrifugation (6000xg for 20 min) and lysed with B-PER Bacterial Protein Extraction Reagent (Thermo Fischer) supplemented with DNAse I (GoldBio). Lysate was incubated at 60°C for 20 min and cleared by centrifugation at 14,000xg for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 μg/mL leupeptin) containing 1× ProBlock™ Protease Inhibitor Cocktail-50 (GoldBio) and processed as previously described[17] ...
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bioRxiv - Cancer Biology 2022Quote: ... The beads bound with biotinylated cell surface proteins were resuspended in 50 mM Tris (pH 8.5) + 4 M urea + 10 mM TCEP (Gold Biotechnology, TCEP10) and 20 mm IAA (VWR ...
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bioRxiv - Molecular Biology 2021Quote: ... (1 mg/ml, Gold Biotechnology). SC-Leu+MPA media is minimal media of SC-Leu supplemented with mycophenolic acid (20 ug/ml ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM AEBSF (Gold Biotechnology), 1 mM Benzamidine (Sigma-Aldrich) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1 mM IPTG (GoldBio I2481C), 400 μM erythromycin ...
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bioRxiv - Plant Biology 2021Quote: ... and 1 mM DTT (GoldBio). Protein concentration was measured using the Bradford method (Bradford ...
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bioRxiv - Biophysics 2023Quote: ... 1% of DDM detergent (GoldBio) was added to the lysed cell and the cells was incubated for 1 h at 4°C to extract the membrane protein ...