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Citations for Gold Biotechnology :
1 - 50 of 288 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... at 1 mg/mL and elastase at 6 U/mL (E-240, Goldbio) for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... 20 mM KCl and home-made protease inhibitor cocktail (500 μM AEBSF, 1 μM E-64, 1 μM Leupeptin, 150 nM Aprotinin) (Gold Biotechnology). Total cellular membranes were harvested by ultracentrifugation ...
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bioRxiv - Biochemistry 2022Quote: ... protein expression was induced with 1 mM IPTG (isopropyl β-D-1-thiogalactopyranoside; GoldBio) followed by expression for 17 hr at 25°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein expression was induced by adding 1 mM IPTG (GoldBio) and grown at 37°C for 5 h with agitation.
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bioRxiv - Biochemistry 2021Quote: ... Lipin 1 and lipin 1 ΔM-Lip proteins were purified using Ni-NTA resin (GoldBio). Eluted protein was applied to Streptactin-XT resin equilibrated with equilibration buffer (100 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... Amersham) and protein expression induced with 1 mM final IPTG (Goldbio) for 4 hr at 37 °C ...
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bioRxiv - Biophysics 2020Quote: ... and protein expression was induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (Goldbio, St Lousi, MO, USA). After induction ...
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bioRxiv - Biochemistry 2021Quote: ... and protein expression was induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (Goldbio, St Lousi, MO, USA). After induction ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein expression was induced for ∼6 h by 1 mM isopropyl β-a-D-1-thiogalactopyranoside (IPTG; GoldBio) in an 18 °C shaker ...
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bioRxiv - Molecular Biology 2021Quote: ... Cultures were cold-shocked on ice for 1 hour and protein expression was induced with 1 mM IPTG (GoldBio). Culture induction was continued for 16 hours 18 °C while shaking ...
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bioRxiv - Biochemistry 2020Quote: ... Induction of protein expression was induced by addition of 1 mM IPTG (Gold Biotech) and cells were grown overnight at 18°C ...
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bioRxiv - Biochemistry 2022Quote: ... followed by protein induction with 0.5 mM isopropyl ß-D-1-thiogalactopyranoside (Gold Biotechnology) at 18°C for 16 hrs ...
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bioRxiv - Biophysics 2019Quote: ... protein expression was then induced with Isopropyl-β-D-1-thiogalactopyranoside 0.1 mM (GoldBio, Canada) for 12 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... 4.5 mg E1D1 antibody was incubated with 1 mL Protein A resin (GoldBio, P-400) with rotation at room temperature for 30 minutes and then washed thoroughly with PBS ...
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DeCOIL: Optimization of Degenerate Codon Libraries for Machine Learning-Assisted Protein EngineeringbioRxiv - Bioengineering 2023Quote: ... Protein expression is induced with the addition of 50 μL of IPTG (GoldBio 367-93-1) to a 1 mM final concentration ...
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bioRxiv - Microbiology 2020Quote: ... alongside a protein molecular weight marker (BLUEstain protein ladder, Gold Biotechnology). Gels were run at 100 V for 15 min and then 150 V for 40 min in NuPAGE MES SDS running buffer and the proteins were subsequently transferred onto PVDF or nitrocellulose membranes using iBlot transfer stacks (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: TNFα (Human) was purchased from GoldBio (#1130-01-100) and suspended in sterilized water to 1 mg/mL concentration ...
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bioRxiv - Immunology 2021Quote: ... RBD proteins were purified using protein A agarose resin (Goldbio CAT# P-400), followed by on-column cleavage with HRV3C protease (made in-house ...
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bioRxiv - Biochemistry 2022Quote: ... protein expression was induced by adding 600 μL of 1 M isopropyl β-D-thiogalactopyranoside (IPTG, Gold Biotechnology) to a final concentration of 1 mM ...
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bioRxiv - Microbiology 2022Quote: ... The virus-serum mix was subsequently added to Vero cells for 48 h before 50 nmoles of D-Luciferin (GoldBio, St Lous, MO, USA) was added to measure luminescence ...
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bioRxiv - Microbiology 2020Quote: ... Secreted hACE2-Fc and mACE2-Fc proteins were purified by protein A chromatography (Goldbio).
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bioRxiv - Immunology 2021Quote: ... 100 ng/ml human IL-21 (GoldBio #1110-21-10), 5 µg/ml TLR9-activator ODN2006 (IDT DNA ...
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bioRxiv - Immunology 2021Quote: ... 100 ng/ml human IL-2 (GoldBio #1110-02-50), 100 ng/ml human IL-21 (GoldBio #1110-21-10) ...
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bioRxiv - Immunology 2023Quote: ... All recombinant human cytokines were purchased from GoldBio (Massachusetts, USA). Following stimulation ...
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bioRxiv - Immunology 2023Quote: ... 100 ng/ml human IL-2 (GoldBio #1110-02-50), and 100 ng/ml human IL-21 (GoldBio #1110-21-10 ...
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bioRxiv - Immunology 2023Quote: ... 100 ng/ml human IL-2 (GoldBio #1110-02-50), and 100 ng/ml human IL-21 (GoldBio #1110-21-10) ...
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bioRxiv - Immunology 2023Quote: ... 100 ng/ml human IL-2 (GoldBio #1110-02-50), 100 ng/ml human IL-21 (GoldBio #1110-21-10) ...
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bioRxiv - Immunology 2023Quote: ... 100 ng/ml human IL-21 (GoldBio #1110-21-10). At day 14 ...
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bioRxiv - Molecular Biology 2024Quote: Human tumor necrosis factor alpha (TNFα, GoldBio #1130-01-100), Interferon alpha (IFNα ...
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bioRxiv - Immunology 2024Quote: ... 100 ng/mL human IL-2 (GoldBio #1110-02-50), and 100 ng/mL human IL-21 (GoldBio #1110-21-10 ...
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bioRxiv - Microbiology 2021Quote: ... Lysate was centrifuged at 18,000xg for 1 hr and the supernatant containing soluble protein was bound to nickel agarose beads (Gold Biotechnology), washed with buffer containing 25mM Tris HCl (pH 7.5) ...
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bioRxiv - Cell Biology 2023Quote: ... Bacteria grew at 37°C and protein induction occurred at 20°C for overnight upon addition of 0.1mM isopropyl D-1-thigalactopyranoside (IPTG; Goldbio, I2481C) to the bacterial culture at mid-log phase (OD600nm=0.6) ...
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bioRxiv - Cancer Biology 2020Quote: Human recombinant IL-1α (GoldBio, St. Louis, MO; 1110-01A-100), IL-1β (GoldBio ...
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bioRxiv - Cancer Biology 2019Quote: Human recombinant IL-1α (GoldBio, St Louis MO; 1110-01A-100) and IL-1β (GoldBio ...
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bioRxiv - Immunology 2023Quote: ... and 100 ng/ml human IL-21 (GoldBio #1110-21-10) to promote expansion and differentiation of B cells into antibody-secreting cells ...
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bioRxiv - Immunology 2023Quote: ... and 100 ng/ml human IL-21 (GoldBio #1110-21-10), without 3T3 cells ...
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bioRxiv - Immunology 2024Quote: ... and 100 ng/mL human IL-21 (GoldBio #1110-21-10) to promote expansion and differentiation of B cells into antibody-secreting cells ...
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bioRxiv - Biophysics 2019Quote: ... at which point protein expression was induced by the addition of 0.5 mM isopropyl 1-thio-β-D-galactopyranoside (Gold Biotechnology). Temperature was reduced to 16°C for overnight expression ...
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bioRxiv - Bioengineering 2023Quote: ... Human hairs were embedded in a 4% agarose block (A-204-25, GoldBio), and the block was placed in the water-filled customized container during imaging ...
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bioRxiv - Molecular Biology 2021Quote: Purification of the mouse lectin-mIgG2a fusion proteins was performed using Protein A agarose resin (Gold Biotechnology, P-400-5). The protein A beads were pelleted at 150g for 5 min and washed once with 1x binding buffer (0.02 M Sodium Phosphate ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein expression was induced with IPTG (GoldBio, I2481C) at 16°C for 18 hours ...
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bioRxiv - Cell Biology 2022Quote: KO2 cells of an early passage were infected with lentiviruses expressing HMGB2- eYFP or FOXM1-eYFP fusion proteins and selected with 20 µg/ml hygromycin B (Goldbio Biotechnology, H-270-1). Pools of cells were then cultured in the presence of hygromycin ...
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bioRxiv - Immunology 2021Quote: ... followed by purification with protein A agarose resin (GoldBio). Expi293F cells were cultured in Expi293 Expression Medium (Gibco ...
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bioRxiv - Biophysics 2020Quote: ... Protein expression was induced by adding 0.2 % arabinose (Goldbio) for 3 hr at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... and mAbs were purified with protein A agarose (GoldBio).
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bioRxiv - Immunology 2020Quote: ... and incubated overnight with Protein A Agarose Resin (GoldBio). The resin was collected in a chromatography column ...
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bioRxiv - Microbiology 2023Quote: ... and incubated overnight with Protein A Agarose Resin (GoldBio). The resin was collected in a chromatography column ...
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bioRxiv - Microbiology 2023Quote: ... and incubated overnight with Protein A Agarose Resin (GoldBio) at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... or Protein A Agarose Resin (Gold Biotechnology, P-400-5). Beads and antibody were incubated and rotated at room temperature for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Bulk IgGs were captured by protein A agarose resin (GoldBio) at 4°C rotating overnight ...