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Citations for Gold Biotechnology :
51 - 100 of 304 citations for 7 chloro 5 methylpyrazolo 1 5 A pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ProBlock Gold Mammalian Protease Inhibitor Cocktail (Gold Biotechnology, Cat. # GB-331-5)
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bioRxiv - Biochemistry 2021Quote: ProBlock Gold Mammalian Protease Inhibitor Cocktail (Gold Biotechnology, Cat. # GB-331-5)
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bioRxiv - Synthetic Biology 2023Quote: ... purified using Nickel NTA agarose beads (Catalog No. H-350-5, GoldBio), and eluted (500 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM MgCl2 and 2 mM Tris (2-carboxyethyl) phosphine (TCEP, GoldBio).
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bioRxiv - Biochemistry 2023Quote: ... coli and isolated using nickel agarose beads (Goldbio, Cat# H-350-5). 5 μM of HaBlap was mixed with 50 μM nitrocefin in 5 mM phosphate buffer pH 7 ...
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bioRxiv - Molecular Biology 2023Quote: ... each plate was sprayed with 1 milliliter of an aqueous solution of 5 mM firefly luciferin (Gold Biotechnology, St Louis, MO) and 0.01% (vol/vol ...
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bioRxiv - Microbiology 2023Quote: ... after completion of virus infection virus containing media was replaced with media containing 5 µg/ml MTT reagent (Thiazolyl blue tetrazolium bromide, cat no.298-93-1, Gold biotechnology). Incubate at 37°C for 3-4 hr and then remove MTT containing media and add 100 µl of DMSO to each well to dissolve the formazan crystals formed ...
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bioRxiv - Biochemistry 2019Quote: ... wells were washed 3x with Buffer A and blocked with 5% BSA (GoldBio) in HBS with 2 mM Mg2+ ...
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bioRxiv - Physiology 2023Quote: ... 5 mL of MS-EGTA buffer with 0.2% BSA (Goldbio #A-421-250) is added to the tube to stop the trypsin reaction ...
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bioRxiv - Physiology 2023Quote: ... 5 mL of MS-EGTA buffer with 0.2% BSA (Goldbio #A-421-250) is added to the tube to stop the trypsin reaction ...
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bioRxiv - Microbiology 2021Quote: ... Ampicillin sodium (cat #A-301-5) was purchased from Gold Biotechnology (St. Louis, MO).
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bioRxiv - Cell Biology 2021Quote: ... Successful integration was positively selected for using G418 (GoldBio Cat no. G-418-5); animals retaining an extrachromosomal array were killed by heat-shock (2 hours ...
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bioRxiv - Biochemistry 2022Quote: ... the cleared cell lysate was loaded onto a gravity column with 1 mL bed volume of Glutathione agarose resin (Gold Biotechnology, #G-250-5). The resin was extensively washed ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were probed with the primary antibodies listed in Supplemental Table 1 overnight in 5% bovine serum albumin (Gold Biotechnology, Cat.# A-420-100) in TBST ...
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bioRxiv - Genomics 2020Quote: AEBSF (Gold Biotechnology CAS#30827-99-7) is added to the Proteinase K (NEB Proteinase K #P8107S ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were applied to GST agarose column (Glutathione agarose resin, GoldBio Cat# G-250-5) and washed 3 times in wash buffer 1x TBS + 1mM DTT) ...
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bioRxiv - Biophysics 2022Quote: ... Tris-(carboxyethyl) phosphine hydrochloride (TCEP) and adenosine-5’-triphosphate (ATP) disodium trihydrate were purchased from GoldBio. Imidazole was purchased from Acros Organics ...
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bioRxiv - Cancer Biology 2021Quote: ... the medium was supplemented with 100 μg/ml hygromycin (#H-270-5; Goldbio, St Louis MO). For serum stimulation ...
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bioRxiv - Bioengineering 2023Quote: ... Bioluminescence images were taken once weekly 5 min following injection of D-luciferin (Gold Biotechnology, US) with an in vivo imaging system (IVIS Spectrum ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% (w/v) bovine serum albumin (BSA; Gold Biotechnology, A-420-100) in tris-buffered saline (TBS ...
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bioRxiv - Physiology 2022Quote: ... N2 worms were egg-prepped onto solid NGM with 50 μg/ml carbenicillin (GoldBio, C-103-5) and HT115(DE3 ...
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bioRxiv - Immunology 2020Quote: ... Clarified cell supernatant containing recombinant antibodies was passed over Protein A Agarose (Goldbio Cat# P-400-5), followed by extensive washing with PBS ...
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bioRxiv - Neuroscience 2020Quote: Each Tg(Gfap-luc) animal was given 5 mg (100 μL of 50 mg/mL) D-luciferin (GoldBio) in saline by intraperitoneal injection ...
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bioRxiv - Cell Biology 2022Quote: ... the procedure was similar except the culture media also contained 5ng/ml GM-CSF (GoldBio # 1320-03-5) to stimulate microglial proliferation ...
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bioRxiv - Developmental Biology 2023Quote: ... plants were incubated with an aqueous solution of 100 µg/ml Carbenicillin (GoldBio, C-103-5, lot # 0129.091814A) for 30 minutes.
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bioRxiv - Biophysics 2021Quote: ... and resuspended in assay buffer (HBSS with 20 mM HEPES pH 7.45) with 5 μg/mL freshly prepared coelenterazine 400a (GoldBio). Cells were then placed in white-walled ...
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bioRxiv - Biochemistry 2022Quote: ... then the cleared bacterial lysate was passed through a 0.22 μm PES filter (Genessee Scientific) and then applied to a 5 mL packed Ni-NTA agarose beads (Goldbio) equilibrated with Lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... with all purification buffers for full-length WT NEMO also containing 5 mM Tris(2-carboxyethyl)phosphine (TCEP) (GoldBio) to minimize the formation of disulfide0bonded aggregates ...
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bioRxiv - Biochemistry 2023Quote: ... ten-fold serial dilutions were plated onto synthetic complete agar lacking histidine and containing 0.1% w/v 5-fluoroorotic acid (GoldBio). Plasmid shuffling experiments involving Use1 were conducted in an analogous manner ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were transferred onto fresh media and treated with 100 µg/ml Carbenicillin (GoldBio, C-103-5, lot # 0129.091814A).
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bioRxiv - Biophysics 2024Quote: ... Cleared lysate was loaded onto lysis buffer-equilibrated Ni-NTA resin (GoldBio, 5 mL resin per 50 mL lysate) and rocked gently for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: Purification of the mouse lectin-mIgG2a fusion proteins was performed using Protein A agarose resin (Gold Biotechnology, P-400-5). The protein A beads were pelleted at 150g for 5 min and washed once with 1x binding buffer (0.02 M Sodium Phosphate ...
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bioRxiv - Microbiology 2020Quote: ... The protein was eluted with 5 mL of elution buffer (20 mM TEA, 50 mM KCl, 500 mM imidazole (GoldBio), pH 7) ...
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bioRxiv - Microbiology 2020Quote: ... The protein eluted and collected in 5-1mL aliquots of elution buffer (50 mM NaH2PO4, 300 mM NaCl, and 250 mM imidazole (GoldBio) (pH 8.0)) ...
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bioRxiv - Developmental Biology 2022Quote: ... 50 μL of DNase (stock: 5 mg/mL; final concentration: 0.025 mg/mL or 50 U/ml, GoldBio, D-301), and 100 μL of Collagenase Type I (stock ...
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bioRxiv - Cell Biology 2022Quote: ... animals were grown on 6 cm NGM plates that were supplemented with 250 μl of 5 mg/ml of hygromycin B (GoldBio) in M9.
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bioRxiv - Biochemistry 2022Quote: ... #DF0446-07-5) or 7H10 (Difco, cat# DF0627-17-4) plates containing 50 ug/mL hygromycin (GoldBio, cat. #H-270) and incubated at 37° C for 3-5 days ...
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bioRxiv - Biochemistry 2024Quote: ... Two-hundred μl of cells were mixed with 200 μl of 5 mM D-luciferin K+ salt (GoldBio, LUCK-100) diluted in Tyrode’s buffer and incubated at 28 °C for 15 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were resuspended in 3 ml assay buffer (HBSS buffer with 0.01% BSA and 5 mM HEPES, pH 7.4) containing 10 μM coelenterazine (Goldbio, Cat. no: CZ05) at final concentration ...
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bioRxiv - Cancer Biology 2022Quote: ... recipe of FACS buffer was RPMI-1640 + 5% FBS + 2% BSA + 50 µg/ml DNase-I (Gold Biotechnology, D-301-500). For all other primary cell staining ...
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bioRxiv - Biochemistry 2022Quote: ... and resuspended in assay buffer (1XHBSS, 5 mM HEPES, pH 7.4, 0.01% BSA) containing 10 μM coelenterazine (GoldBio, Cat no. CZ2.5). Resuspended cells were seeded in a white flat bottom 96-well plate (100 μl well-1) ...
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bioRxiv - Biochemistry 2023Quote: ... and resuspended in the NanoBiT assay buffer (HBSS containing 0.01% BSA, 5 mM HEPES, pH 7.4, and 10 μM coelenterazine (GoldBio, Cat no: CZ05). After resuspension ...
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bioRxiv - Microbiology 2022Quote: ... samples were diluted in 7 mL of 0.1 M Tris (Gold Biotechnology), pH 7.5 containing 8 M urea and 25 mM 2-iodoacetamide (both from Acros Organics ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed with lysis buffer (50 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM n-Dodecyl-B-D-maltoside (DDM) (GoldBio, catalog #: DDM-5)) supplemented with protease inhibitor cocktail (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... were transformed and plated on LB Agar (Fisher BioReagents Cat#: BP1425-500) containing 50 µg/mL carbenicillin (GoldBio Cat#: C-103-5) and incubated overnight at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... The G418-resistance cassettes were transformed into FY4 (MATa) and selected on YPD + G418 (400 µg/mL; Gold Biotechnology #G-418-5) plates ...
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bioRxiv - Genetics 2021Quote: ... After extraction for 30 min at RT samples were centrifuged 600g 3 min and supernatants were precleared with 15μl of protein A agarose beads (Goldbio, cat. P-400-5) for 2 hours at RT ...
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bioRxiv - Synthetic Biology 2021Quote: ... and to this 0.85 mL of liquid (70 °C) top agar (2XYT media + 0.6% agar) supplemented with 2% Bluo-Gal (GoldBio, CAS #97753-82-7) was added and mixed by pipetting up and down once ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (GoldBio) was added to induce protein expression at 37 °C for 4 h ...
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bioRxiv - Cell Biology 2022Quote: ... and rifampicin (GoldBio #R-120-1, 100 μg mL-1). Bacterial treatments for actin dynamics analysis were conducted following previously described methods36 ...