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Citations for Gold Biotechnology :
101 - 148 of 148 citations for 7 Chloro 1H pyrrolo 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and cultured axenically at 21°C in HL5 media (Froquet et al., 2009) supplemented with 300 µg/mL Streptomycin Sulfate (Gold Biotechnology) on 10 cm plates ...
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bioRxiv - Plant Biology 2021Quote: ... The GUS staining buffer was then supplemented with fresh 2 mM X-Gluc (GoldBio, MO) and vacuum infiltrated again ...
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bioRxiv - Genomics 2021Quote: ... Nanoluciferase activity was evaluated from the conditioned medium using a non-lytic 2× coelenterazine (Goldbio) reagent as previously described (91) ...
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bioRxiv - Neuroscience 2022Quote: ... followed by a second elution with 45 µl 2% n-β-dodecylmaltoside (DDM) (Gold Biotechnologies) for 5 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 5mM glycerol 2-phosphate and 1X ProBlock Gold Protease Inhibitor Cocktail (GoldBio, St. Louis, MO), and cooled to 4°C before use ...
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bioRxiv - Plant Biology 2024Quote: ... seedlings were rinsed with 1/2MS with 10 µM Timentin (Gold Biotechnology, Cat# T104-2), transferred into a fresh 1/2MS medium ...
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bioRxiv - Physiology 2020Quote: ... in Krebs-Ringer-HEPES (KRH; pH 7.4) buffer containing 3% fatty acid-free bovine serum albumin (BSA; Gold Biotechnology, St. Louis, NJ), 1 g/L glucose ...
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bioRxiv - Bioengineering 2023Quote: ... Images were acquired 10 min after intraperitoneal injection of 3 mg d-Luciferin Potassium Salt (Gold Biotechnology, Inc., St Louis MO, USA) in 200 μL 0.1 M phosphate buffered saline ...
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bioRxiv - Molecular Biology 2023Quote: ... in Krebs-Ringer-HEPES (KRH; pH 7.4) buffer containing 3% fatty acid-free bovine serum albumin (BSA; Gold Biotechnology, St. Louis, NJ), 1 g/L glucose ...
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bioRxiv - Microbiology 2020Quote: ... prepared in PBS and then incubated overnight at 37°C (without carbon dioxide) with freshly prepared staining solution (1mg/ml of X-gal (GoldBio Technology, USA) in 40 mM citric acid/sodium phosphate ...
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bioRxiv - Molecular Biology 2020Quote: ... and grown at 37 °C in LB Broth Miller (EMD) overnight with the respective antibiotics (100 μg/ml ampicillin (Gold Biotechnology, Inc.)) for pAS06 plasmid and 30 μg/ml chloramphenicol (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2022Quote: Individual 6-day-old seedlings expressing a CCA1::LUC reporter grown under LD cycles at 22°C were arrayed on 1/2x MS + 1% Sucrose plates and sprayed with 5mM luciferin (GoldBio, Olivette, MO) prepared in 0.01% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... were transformed and plated on LB Agar (Fisher BioReagents Cat#: BP1425-500) containing 50 µg/mL carbenicillin (GoldBio Cat#: C-103-5) and incubated overnight at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... the temperature was reduced to 20 °C and protein expression was induced with the addition of IPTG (GoldBio, St. Louis, MO, USA) to a final concentration of 1 mM ...
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bioRxiv - Biophysics 2021Quote: ... the GltPh proteins were reduced with 500 µM tris(2-carboxy-ethyl) phosphine (TCEP, Gold Biotechnology) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: The cytokine used for Ba/F3 culturing was 10 ng/mL murine IL-3 (Cat.#31310-03-10; Gold Biotechnology, St Louis, US).
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bioRxiv - Genetics 2021Quote: ... After extraction for 30 min at RT samples were centrifuged 600g 3 min and supernatants were precleared with 15μl of protein A agarose beads (Goldbio, cat. P-400-5) for 2 hours at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... the following were added directly to worm plates 3 days after injection: HygR selection −100 ul of 20 mg/ml hygromycin B (GoldBio, St. Louis, MO), NeoR selection - 500 ul of 25 mg/ml G418 disulfate (Sigma ...
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bioRxiv - Bioengineering 2024Quote: ... Mice were imaged on the IVIS every 3-4 days after being sedated with Isoflurane and intraperitoneal injections of ∼120 mg/kg D-luciferin (GoldBio, St. Louis, MI). Mice were injected with 100,000 IU of human IL-2 (National Cancer Institute ...
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bioRxiv - Biochemistry 2022Quote: ... in the medium with the exception of the 293T-based CAPN5-3×FLAG stable cell line that was selected and maintained with 200 µg/ml hygromycin (Gold Biotechnology, H-270-1).
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bioRxiv - Synthetic Biology 2023Quote: ... CAS NO 3483-12-3), isopropyl-β-thiogalactopyranoside (IPTG, CAS NO 367-93-1) and bovine serum albumin (BSA) were obtained from Gold Biotechnology (Burlington, MA). Alexa Fluor 594 C5 maleimide was purchased from BroadPharm (San Diego ...
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bioRxiv - Biochemistry 2021Quote: ... All lysis buffers used were supplemented with 1X protease inhibitor cocktail11 and 2 mM AEBSF (Gold Biotech). A list of constructs used can be found in Supplementary Table 2.
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bioRxiv - Cell Biology 2021Quote: ... 200 µl diluted cells (2 x104 cells) and 50 µl (8 mg/ml) D-luciferin (GoldBio, USA) were loaded into each well ...
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bioRxiv - Molecular Biology 2022Quote: ... 120 µg protein was mixed with substrate containing 15mM ONPG (2-Nitrophenyl-β-D-galactopyranoside, Goldbio, #N27510), 5mM DTT ...
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bioRxiv - Bioengineering 2023Quote: ... The cultures were grown at 37°C at 220 rpm and induced at an OD600 of 1-1.2 using 1 mM isopropylthio-β-galactoside (IPTG) (GoldBio, St. Lois, MO, USA). Cultures for fluorescence imaging were grown C aerobically at 16° for 16h post-induction ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was filtered and then incubated with nutation at 4°C for 1 hr with Ni-NTA agarose (Gold Biotechnology #H-350-100) that was pre-equilibrated with lysis buffer ...
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bioRxiv - Biophysics 2019Quote: ... 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (HEPES) and Dithiothreitol (DTT) were purchased from GoldBio (St Louis, MO, US). Ethylene Glycol-bis(β-aminoethyl ether)-N,N,N′,N′-Tetraacetic Acid (EGTA) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Inducers and antibiotics were used at the following working concentrations: 2 mg/mL L-arabinose (GoldBio A-300), 1 mM IPTG (GoldBio I2481C) ...
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bioRxiv - Physiology 2020Quote: ... were washed twice with PBS and starved of serum for 2 h in DMEM with 0.1% BSA (GoldBio). Following starvation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were washed once with 1× PBS and 75 μL of 2 mM D-luciferin (LUCK-1G, Goldbio) in Leibovitz’s L-15 medium (21083-027 ...
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bioRxiv - Bioengineering 2023Quote: ... Inducers and antibiotics were used at the following working concentrations: 2 mg/ml L-arabinose (GoldBio, A-300), 1 mM IPTG (GoldBio ...
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bioRxiv - Microbiology 2022Quote: ... coli cultures were grown at 37 °C on LC medium containing either kanamycin or spectinomycin (50 or 100 μg/ml, respectively; Gold Biotechnology, Inc., St. Louis, MO) and used as the host for recombinant plasmids made via sequence and ligation-independent cloning (SLIC ...
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bioRxiv - Molecular Biology 2023Quote: ... with all purification buffers for full-length WT NEMO also containing 5 mM Tris(2-carboxyethyl)phosphine (TCEP) (GoldBio) to minimize the formation of disulfide0bonded aggregates ...
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bioRxiv - Cancer Biology 2023Quote: ... and imaged within 2-4 minutes after retro-orbital injection of 1.5 mg D-luciferin (GoldBio, Cat: LUCK-10G) dissolved in 100 µL PBS ...
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bioRxiv - Biochemistry 2023Quote: ... The pellet was resuspended in a solubilization buffer containing 2% n-Dodecyl-B-D-Maltoside (DDM, GoldBio, Cat. # DDM10), 350 mM NaCl in PBS ...
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bioRxiv - Plant Biology 2023Quote: ... 75 µL of water was replaced with 25 µL of a 2 mM Luciferin (GoldBio; Catalog number: LUCNA-100) solution ...
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bioRxiv - Microbiology 2024Quote: Mice received an IP injection of 100 μl of a 2% D-luciferin solution (Goldbio cat #115144-35-9), were anaesthetized with isoflurane gas ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Equal volumes of each sample were loaded onto a 4-12% SDS-PAGE gel along with the BLUEstainTM 2 (GoldBio) protein ladder ...
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bioRxiv - Cancer Biology 2022Quote: ... recipe of FACS buffer was RPMI-1640 + 5% FBS + 2% BSA + 50 µg/ml DNase-I (Gold Biotechnology, D-301-500). For all other primary cell staining ...
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bioRxiv - Systems Biology 2023Quote: ... Permeabilized cells were washed twice with 1X PBS then resuspended in 0.01% Triton-X-100/2% BSA/1X PBS (GoldBio #A-420) at room temperature on a shaker for 1 hour or overnight at 4 °C to block non-specific protein binding ...
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bioRxiv - Cell Biology 2023Quote: ... into a centrifuge tube and then lysed with lysis buffer (50mM Tris, pH 7.5, 150mM NaCl, and 2 mM Dodecyl-B-D-maltoside (DDM) (GoldBio, catalog #: DDM5) supplemented with complete protease inhibitor cocktail (Roche #04693116001) ...
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bioRxiv - Biochemistry 2023Quote: ... Luminescence was measured for 2 s in a Lumat LB 9507 (Berthold Technologies) after injection of 100 µl 250 nM Luciferin (Gold Biotechnology). Luciferase Refolding served as a measure for disaggregation activity ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed with lysis buffer (50 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM n-Dodecyl-B-D-maltoside (DDM) (GoldBio, catalog #: DDM-5)) supplemented with protease inhibitor cocktail (Roche) ...
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bioRxiv - Biochemistry 2023Quote: ... 20 mM of 4-(2- hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) pH 7.4 and D-luciferin (0.5 mg ml-1) (GoldBio, Cat. no.: LUCNA-1G). Harvested cells were then seeded in an opaque flat bottom white 96 well cell culture plate (SPL life sciences ...
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bioRxiv - Cell Biology 2023Quote: Cells were harvested and lysed with lysis buffer (50 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM n-Dodecyl-β-D-maltoside (DDM) (GoldBio, catalog #: DDM5)) supplemented with complete protease inhibitor cocktail (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... or fog-2(q71) along with approximately 30 wild type males were placed on RNAi plates [MYOB containing 1mM IPTG (GoldBio, Cat. No. I2481C) and seeded with the appropriate dsRNA expressing HT115 cells] ...
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bioRxiv - Cancer Biology 2022Quote: ... Spectral bioluminescent images of live cells growing in vitro were acquired 2 minutes after adding 150 ug/ml of D-Luciferin (Goldbio, Saint Louis, MO). Sequential images were acquired through optical filters ranging from 520 to 660nm at 20nm intervals ...
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bioRxiv - Microbiology 2021Quote: ... Fecal samples were collected regularly over 2-6 weeks and plated onto SAB and SAB with nourseothricin (100 μg/mL, Gold Biotechnology, N-500-1) plates.