Labshake search
Citations for Gold Biotechnology :
301 - 350 of 363 citations for 7 Bromo 4 chlorothieno 3 2 d pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... resuspended in assay buffer (20mM HEPES pH 7.4, 1X Hank’s Balanced Salt Solution/ HBSS and 0.5mg/mL D-luciferin (GoldBio, Cat. no: LUCNA-1G) and seeded in 96-well plates at a density of 100,000 cells/well ...
-
bioRxiv - Cancer Biology 2024Quote: ... Tumor volume was monitored through bioluminescence using IVIS Spectrum apparatus (Caliper Life Sciences) after i.p injection of D-luciferin 150mg/Kg (GOLDBIO #LUCK-1G). Mice were sacrificed when manifested more than 20% of body weight loss or neurological symptoms ...
-
bioRxiv - Neuroscience 2023Quote: ... Bacteria were grown to an OD600 of 0.6 and induced with 500 µM isopropyl β-D-1-thioga-lactopyranoside (IPTG) (GoldBio, I2481C) for 18 h at 18°C ...
-
bioRxiv - Cell Biology 2021Quote: ... a solution of 2% low-melt agarose (Gold Biotechnology, A-204) was made in L-15 media (Gibco ...
-
bioRxiv - Cell Biology 2023Quote: ... selection was performed with 2 μg/mL puromycin (GoldBio, P-600). The protocol for generating ATG3 KO Hela cells stably expressing HaloTag-LC3B is available at dx.doi.org/10.17504/protocols.io.5qpvo3xq9v4o/v1.
-
bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in 2 % BSA (A-420-500 GoldBio) in TBS-T to the indicated concentration and incubated with membranes overnight at 4C with rocking ...
-
bioRxiv - Biochemistry 2022Quote: ... cells were detached from the plates, resuspended in assay buffer (1XHBSS, 20 mM HEPES, pH7.4) containing D-luciferin (0.5 mg ml-1, GoldBio, Cat. no. LUCNA-1G) and seeded into 96 well white plates (Corning ...
-
bioRxiv - Microbiology 2020Quote: ... ATP-free or ATP-containing (150 μM ATP) substrate solutions were prepared containing 150 μg/mL D-Luciferin (Goldbio, St. Louis, MO), 100 mM Tris pH 8 ...
-
bioRxiv - Biochemistry 2020Quote: ... the culture was expanded 1:100 up to 2L cultures in Luria broth and induced at A600 ∼0.60 with 0.5 mM isopropyl β-D-thiogalactopyranoside (GoldBio, St. Louis MO) for 24 h at 16°C ...
-
bioRxiv - Cell Biology 2021Quote: NSG mice transplanted with GAPDHLuciferase/wt and INSGFP/wt double reporter hESCs lines were i.p injected with 150mg/kg body weight of D-luciferin potassium salt (Gold Biotechnology, luck-2G) in PBS 15 minutes before imaging on a IVIS spectrum optical imaging system (PerkinElmer) ...
-
bioRxiv - Cell Biology 2023Quote: ... were spotted onto LB plates supplemented with 100 µg/mL ampicillin with or without 1 mM isopropyl β-D-1-thiogalacopyranoside (IPTG, Gold Biotechnology). After drying ...
-
bioRxiv - Biophysics 2023Quote: ... resuspended in the assay buffer (20mM HEPES pH 7.4 and 1X HBSS) containing 0.5mg ml-1 of D-luciferin (GoldBio, Cat. no: LUCNA-1G) and seeded in 96-well white plates (SPL Life Sciences ...
-
bioRxiv - Cell Biology 2023Quote: PIP Strips (Echelon) were blocked overnight in PBS-T with 3% fatty acid-free bovine serum albumin (GoldBio) at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... containing phosphatase and protease inhibitor cocktail (Goldbio Cat. No. GB-108-2). Western blotting was performed using 40 μg protein/well as previously described (Mohammed et al ...
-
bioRxiv - Biochemistry 2020Quote: Simple Stop™ 2 Phosphatase Inhibitor Cocktail (Gold Biotechnology, Cat. # GB-451)
-
bioRxiv - Biochemistry 2022Quote: ... The digested protein was passed over a 2-mL Ni-HTC (GoldBio) gravity column to separate the tag-free NnlA ...
-
bioRxiv - Biochemistry 2022Quote: Tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl, Gold Biotechnology cat. no. TCEP1)
-
bioRxiv - Cancer Biology 2024Quote: ... Tumor progression was evaluated by luminescence emission on a Xenogen IVIS spectrum after intraperitoneal injection of D-luciferin (Gold Biotechnology, St. Louis, MO) injection according to the manufacturer’s directions ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Single colonies were inoculated into 3 mL of rich M9- glycerol media with kanamycin (34 µg/mL, Gold Biotechnology) and ampicillin (100 µg/mL ...
-
bioRxiv - Biochemistry 2023Quote: ... the clarified supernatant containing RPA4AZP was applied to a 3 mL pre-equilibrated Ni2+-NTA (Gold Biotechnology Inc., USA) gravity column ...
-
bioRxiv - Cancer Biology 2024Quote: ... Blots were blocked for 1 h at RT in 1X TBST + 3% BSA Fraction V (Gold Biotechnology, Olivette, MO) and then incubated in primary antibody diluted in blocking buffer with overnight rocking at 4°C (phosphorylated and total EGFR ...
-
bioRxiv - Bioengineering 2022Quote: ... Cells were co-cultured overnight and luminescence was measured the next morning after addition of 150 μg/ml D-luciferin (Gold Biotechnology Cat# LUCNA-2G) and subsequent incubation for 5 mins at 37°C ...
-
bioRxiv - Microbiology 2022Quote: ... The virus-serum mix was subsequently added to Vero cells for 48 h before 50 nmoles of D-Luciferin (GoldBio, St Lous, MO, USA) was added to measure luminescence ...
-
bioRxiv - Molecular Biology 2021Quote: ... strains were selected on yeast peptone 2% dextrose (YPD) plates containing nourseothricin (GoldBio), hygromycin B (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2024Quote: ... The supernatants were applied to a 2 ml nickel agarose (high density, GoldBio) for binding for 1 hour ...
-
bioRxiv - Genetics 2022Quote: ... 500 μl of 4 mg/ml stock of Hygromycin solution (Gold Biotechnology, catalog no. H-270-1) was topically added to the bacterial lawn of injection plates to select for transgenic (F1 ...
-
bioRxiv - Microbiology 2023Quote: ... Lysates were clarified via centrifugation and applied to 4 mL of pre-equilibrated Ni+-NTA resin (GoldBio) in a gravity flow column ...
-
bioRxiv - Plant Biology 2020Quote: ... The center 8-10 cm of the second leaf from 7-9 plants were cut into thin strips perpendicular to the veins and immediately submerged in 10 mL protoplasting solution (0.6 M mannitol, 10 mM MES, 15 mg/mL cellulase R-10 [GoldBio], 3 mg/mL Macerozyme R-10 [GoldBio] ...
-
bioRxiv - Plant Biology 2021Quote: ... The center 8 to 10 cm of the second leaf from 10 to 12 plants were cut into thin strips perpendicular to the veins and immediately submerged in 10 mL protoplasting solution (0.6 M mannitol, 10 mM MES, 15 mg/mL cellulase R-10 [GoldBio], 3 mg/mL Macerozyme R-10 [GoldBio] ...
-
bioRxiv - Cell Biology 2019Quote: ... after a 3 minutes pre-incubation with of 2.5 µM coelenterazine H (BRET1 for V2R β-arrestin interaction) from Goldbio or coelenterazine 400a (BRET2 for CAAX and FYVE assays ...
-
bioRxiv - Neuroscience 2023Quote: ... containing ProBlock Gold Extra Strength Protease Inhibitor Cocktail Kit (GoldBio, Cat. #: GB-116-2). The cortical lysate was then aliquoted into a 96 well plate and stored at −80°C.
-
bioRxiv - Bioengineering 2019Quote: ... the following antibiotic concentrations are used (both plates and in culture): 100 μg/ml ampicillin (Ap, GoldBio; CAS#69-52-3); 50 μg/ml kanamycin (Kn ...
-
bioRxiv - Cancer Biology 2021Quote: ... Live-cell luciferase readings were performed for 3 consecutive days by adding 150 μg/mL luciferine (GOLDBIO, Catalog No. LUCK-500) and measuring luminescence in a Tecan Infinite F500 reader (Tecan).
-
bioRxiv - Biochemistry 2020Quote: ... and His SUMO tags were cleaved with Ulp1 protease overnight at 4°C in Buffer C (25mM Tris-HCl pH 8.5, 150mM KCl, 2mM TCEP (GoldBio), 50mM Imidazole ...
-
bioRxiv - Plant Biology 2021Quote: ... The GUS staining buffer was then supplemented with fresh 2 mM X-Gluc (GoldBio, MO) and vacuum infiltrated again ...
-
bioRxiv - Genomics 2021Quote: ... Nanoluciferase activity was evaluated from the conditioned medium using a non-lytic 2× coelenterazine (Goldbio) reagent as previously described (91) ...
-
bioRxiv - Neuroscience 2022Quote: ... followed by a second elution with 45 µl 2% n-β-dodecylmaltoside (DDM) (Gold Biotechnologies) for 5 minutes at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... 5mM glycerol 2-phosphate and 1X ProBlock Gold Protease Inhibitor Cocktail (GoldBio, St. Louis, MO), and cooled to 4°C before use ...
-
bioRxiv - Plant Biology 2024Quote: ... seedlings were rinsed with 1/2MS with 10 µM Timentin (Gold Biotechnology, Cat# T104-2), transferred into a fresh 1/2MS medium ...
-
bioRxiv - Cancer Biology 2020Quote: ... Mice were shaved front and back and Nair was applied to remove remainder of the hair before being IP injected with D-luciferin (Goldbio LUCK-1G, 150mg/kg in 150 mL). Mice are placed in a supine position on the light-tight chamber of the CCD camera imaging unit ...
-
bioRxiv - Physiology 2020Quote: ... in Krebs-Ringer-HEPES (KRH; pH 7.4) buffer containing 3% fatty acid-free bovine serum albumin (BSA; Gold Biotechnology, St. Louis, NJ), 1 g/L glucose ...
-
bioRxiv - Molecular Biology 2023Quote: ... in Krebs-Ringer-HEPES (KRH; pH 7.4) buffer containing 3% fatty acid-free bovine serum albumin (BSA; Gold Biotechnology, St. Louis, NJ), 1 g/L glucose ...
-
bioRxiv - Biochemistry 2022Quote: ... #DF0446-07-5) or 7H10 (Difco, cat# DF0627-17-4) plates containing 50 ug/mL hygromycin (GoldBio, cat. #H-270) and incubated at 37° C for 3-5 days ...
-
bioRxiv - Cell Biology 2023Quote: ... The transformants were selected for 4-5 days in 1 ml of YPD broth with clonNAT (200 µg/mL, GoldBio), resulting in the intermediate MTS-mCherry-GFP1-10-clonNat library ...
-
bioRxiv - Cancer Biology 2023Quote: ... 4×10e7 cfu were plated on-trp Gal/Raf supplemented with 1mg/ml 5-fluoroorotic acid (5-FOA) (Gold Biotechnology) and were grown at 30 degrees for 96 hours ...
-
bioRxiv - Biophysics 2021Quote: ... the GltPh proteins were reduced with 500 µM tris(2-carboxy-ethyl) phosphine (TCEP, Gold Biotechnology) for 30 min at room temperature ...
-
bioRxiv - Cancer Biology 2020Quote: The cytokine used for Ba/F3 culturing was 10 ng/mL murine IL-3 (Cat.#31310-03-10; Gold Biotechnology, St Louis, US).
-
bioRxiv - Genetics 2021Quote: ... After extraction for 30 min at RT samples were centrifuged 600g 3 min and supernatants were precleared with 15μl of protein A agarose beads (Goldbio, cat. P-400-5) for 2 hours at RT ...
-
bioRxiv - Molecular Biology 2024Quote: ... the following were added directly to worm plates 3 days after injection: HygR selection −100 ul of 20 mg/ml hygromycin B (GoldBio, St. Louis, MO), NeoR selection - 500 ul of 25 mg/ml G418 disulfate (Sigma ...
-
bioRxiv - Cancer Biology 2022Quote: ... The beads bound with biotinylated cell surface proteins were resuspended in 50 mM Tris (pH 8.5) + 4 M urea + 10 mM TCEP (Gold Biotechnology, TCEP10) and 20 mm IAA (VWR ...