Labshake search
Citations for Gold Biotechnology :
251 - 300 of 331 citations for 7 Bromo 1 2 3 4 tetrahydronaphthalen 1 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... with 10% (v/v) FBS and 2 mg/mL D-luciferin (Goldbio) and incubated for 2 hours at room temperature (RT ...
-
bioRxiv - Microbiology 2021Quote: ... Lysate was centrifuged at 18,000xg for 1 hr and the supernatant containing soluble protein was bound to nickel agarose beads (Gold Biotechnology), washed with buffer containing 25mM Tris HCl (pH 7.5) ...
-
bioRxiv - Microbiology 2019Quote: ... Yale University] were maintained in Luria-Bertani (LB) medium supplemented with antibiotics as appropriate for plasmid selection [50 µg mL-1 kanamycin (GoldBio), 100 µg mL-1 ampicillin (GoldBio ...
-
bioRxiv - Plant Biology 2019Quote: 0.5g inflorescence tissue per sample was ground with liquid nitrogen and homogenized in lysis buffer (50mM Tris pH 7.5, 150mM NaCl, 5mM MgCl2, 10% glycerol, 1% IGEPAL, 0.5mM DTT, 1mM PMSF, and 1X GoldBio protease inhibitor) for 15 minutes ...
-
bioRxiv - Cell Biology 2021Quote: GST pulldowns were performed by loading 1 µg of purified GST or GST-p130 onto 15 µL of glutathione beads (GoldBio) in NETN (20 mM Tris pH8.0 ...
-
bioRxiv - Developmental Biology 2021Quote: ... containing 5 mL of digestion buffer (1 mL of Dispase [Corning, 354235], 50 μL of DNase [5 mg/mL, GoldBio, D-301] ...
-
bioRxiv - Biochemistry 2021Quote: ... cells were harvested and resuspended in assay buffer containing D-luciferin (0.5 mg ml−1, GoldBio, Cat. no. LUCNA-1G) in 1X HBSS ...
-
bioRxiv - Molecular Biology 2021Quote: ... 0.5% Triton with 500 μM each of potassium ferrocyanide and potassium ferricyanide and infiltrated with wash buffer as above containing 1 mM X-Gluc (GoldBio). Samples were incubated at 37°C in the dark until staining became visible and were cleared with ethanol for imaging ...
-
bioRxiv - Developmental Biology 2023Quote: ... Samples were then incubated in X-gal staining solution (X-gal rinse buffer plus 1 mg/mL X-gal (GoldBio X4281C ...
-
bioRxiv - Cell Biology 2023Quote: ... and added to the cell medium to 1 μM final concentration. D-luciferin sodium salt (Cat. #LUCNA) and coelenterazine (Cat. #CZ) were purchased from GoldBio and resuspended to 100 mM in 10 mM HEPES buffer ...
-
bioRxiv - Microbiology 2023Quote: ... New England Biolabs] were grown at 37 °C in Luria-Bertani (LB) medium supplemented with antibiotics as appropriate for plasmid selection [50 µg mL-1 kanamycin (GoldBio), 25 µg mL-1 chloramphenicol (GoldBio) ...
-
bioRxiv - Genetics 2023Quote: ... and depleted of non-transposed cells by resuspending the pelleted cells in 300 mL of fresh SCD media containing 1 mg/mL of 5-fluoroorotic acid (ZymoResearch) and 0.1 mg/mL of nourseothricin (Gold Biotechnology) and shaking overnight at 30°C ...
-
bioRxiv - Cell Biology 2023Quote: ... Bacteria grew at 37°C and protein induction occurred at 20°C for overnight upon addition of 0.1mM isopropyl D-1-thigalactopyranoside (IPTG; Goldbio, I2481C) to the bacterial culture at mid-log phase (OD600nm=0.6) ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Single colonies were inoculated into 3 mL of rich M9- glycerol media with kanamycin (34 µg/mL, Gold Biotechnology) and ampicillin (100 µg/mL ...
-
bioRxiv - Biochemistry 2023Quote: ... the clarified supernatant containing RPA4AZP was applied to a 3 mL pre-equilibrated Ni2+-NTA (Gold Biotechnology Inc., USA) gravity column ...
-
bioRxiv - Molecular Biology 2021Quote: ... strains were selected on yeast peptone 2% dextrose (YPD) plates containing nourseothricin (GoldBio), hygromycin B (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2024Quote: ... The supernatants were applied to a 2 ml nickel agarose (high density, GoldBio) for binding for 1 hour ...
-
bioRxiv - Biophysics 2019Quote: ... at which point protein expression was induced by the addition of 0.5 mM isopropyl 1-thio-β-D-galactopyranoside (Gold Biotechnology). Temperature was reduced to 16°C for overnight expression ...
-
bioRxiv - Plant Biology 2020Quote: ... Chemiluminescence was detected using the NightSHADE LB 985 system (Berthold) in the presence of 20 mg mL−1 potassium luciferin (Gold Biotech). The LUC/REN ratio was calculated to infer effectiveness of miR775 targeting.
-
bioRxiv - Biophysics 2020Quote: ... 1% of primary culture was inoculated in 750 mL of LB media and induced with 0.3 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Gold Biotechnology) at OD600 ~ 0.6 and further incubated at 16 °C for 14–16 h with constant shaking at 200 rpm ...
-
bioRxiv - Cell Biology 2021Quote: ... cell extraction was carried out as above with the addition of a phosphatase inhibitor cocktail (Simple Stop 1 #GB-450) purchased from Gold Biotechnology, USA ...
-
bioRxiv - Biochemistry 2021Quote: ... Transformed cells were grown at 37°C until OD 0.3 then grown at 18°C until induction at OD 0.6 with 1 mM IPTG (Gold Biotechnology, Olivette, MO). Cells were grown for 15 hours then centrifuged after which the pellet was resuspended in 20 mM Sodium Phosphate pH 8 ...
-
bioRxiv - Biochemistry 2021Quote: ... expression cultures were grown at 37 °C to an optical density (OD600) of 3.0 before induction with 500 μM isopropyl-β-D-1-thiogalactopyranoside (IPTG; Gold Biotechnology) and incubation overnight at 16 °C ...
-
bioRxiv - Cancer Biology 2022Quote: ... Luciferase expression was checked by using in vitro bioluminescence imaging by adding 100 μL of 15 mg mL-1 D-luciferin solution (#LUCNA-1G, Goldbio, USA) to the cells and imaged after 2 min using a Fusion FX system (Vilber ...
-
bioRxiv - Cancer Biology 2023Quote: ... Luc2 and NLuc expression was confirmed by exposing cells seeded in the wells of a flat-bottom white 96-well plate to 1 volume of 15 mg/mL D-luciferin (#LUCNA-1G, Goldbio, USA) or 1 volume of 0.5 mg/mL Nano-Glo In Vivo Substrate ...
-
bioRxiv - Molecular Biology 2023Quote: ... each plate was sprayed with 1 milliliter of an aqueous solution of 5 mM firefly luciferin (Gold Biotechnology, St Louis, MO) and 0.01% (vol/vol ...
-
bioRxiv - Microbiology 2023Quote: ... after completion of virus infection virus containing media was replaced with media containing 5 µg/ml MTT reagent (Thiazolyl blue tetrazolium bromide, cat no.298-93-1, Gold biotechnology). Incubate at 37°C for 3-4 hr and then remove MTT containing media and add 100 µl of DMSO to each well to dissolve the formazan crystals formed ...
-
bioRxiv - Neuroscience 2023Quote: ... Bacteria were grown to an OD600 of 0.6 and induced with 500 µM isopropyl β-D-1-thioga-lactopyranoside (IPTG) (GoldBio, I2481C) for 18 h at 18°C ...
-
bioRxiv - Microbiology 2023Quote: ... Lysates were clarified via centrifugation and applied to 4 mL of pre-equilibrated Ni+-NTA resin (GoldBio) in a gravity flow column ...
-
bioRxiv - Plant Biology 2020Quote: ... The center 8-10 cm of the second leaf from 7-9 plants were cut into thin strips perpendicular to the veins and immediately submerged in 10 mL protoplasting solution (0.6 M mannitol, 10 mM MES, 15 mg/mL cellulase R-10 [GoldBio], 3 mg/mL Macerozyme R-10 [GoldBio] ...
-
bioRxiv - Plant Biology 2021Quote: ... The center 8 to 10 cm of the second leaf from 10 to 12 plants were cut into thin strips perpendicular to the veins and immediately submerged in 10 mL protoplasting solution (0.6 M mannitol, 10 mM MES, 15 mg/mL cellulase R-10 [GoldBio], 3 mg/mL Macerozyme R-10 [GoldBio] ...
-
bioRxiv - Cell Biology 2019Quote: ... after a 3 minutes pre-incubation with of 2.5 µM coelenterazine H (BRET1 for V2R β-arrestin interaction) from Goldbio or coelenterazine 400a (BRET2 for CAAX and FYVE assays ...
-
bioRxiv - Cancer Biology 2021Quote: ... Approximate tumor size was monitored via bioluminescence imaging (Xenogen IVIS Spectrum Imaging System) 1-2x per week following intraperitoneal injection of luciferin reagent (GOLDBIO, #LUCK-1G) at 150mg/kg ...
-
bioRxiv - Biochemistry 2022Quote: ... cells were detached from the plates, resuspended in assay buffer (1XHBSS, 20 mM HEPES, pH7.4) containing D-luciferin (0.5 mg ml-1, GoldBio, Cat. no. LUCNA-1G) and seeded into 96 well white plates (Corning ...
-
bioRxiv - Synthetic Biology 2021Quote: ... coli S17-1 λ pir was routinely grown in Luria Bertani (LB) broth (LabExpress) supplemented with 100 μg/mL carbenicillin (Gold Biotechnology) for plasmid selection.
-
bioRxiv - Biophysics 2023Quote: ... resuspended in the assay buffer (20mM HEPES pH 7.4 and 1X HBSS) containing 0.5mg ml-1 of D-luciferin (GoldBio, Cat. no: LUCNA-1G) and seeded in 96-well white plates (SPL Life Sciences ...
-
bioRxiv - Systems Biology 2023Quote: ... The NAT-resistance cassettes were transformed into FY5 (MATα) and selected on YPD + NAT (200 µg/mL; Gold Biotechnology #N-500-1) plates ...
-
bioRxiv - Neuroscience 2023Quote: ... containing ProBlock Gold Extra Strength Protease Inhibitor Cocktail Kit (GoldBio, Cat. #: GB-116-2). The cortical lysate was then aliquoted into a 96 well plate and stored at −80°C.
-
bioRxiv - Cancer Biology 2022Quote: ... mice were anesthetized with 2.5% isoflurane (Fujifilm Wako) and intraperitoneally injected with 3 mg D-luciferin (Gold Biotechnology Inc., MO, USA) in 200 μl PBS as previously described [52 ...
-
bioRxiv - Bioengineering 2019Quote: ... the following antibiotic concentrations are used (both plates and in culture): 100 μg/ml ampicillin (Ap, GoldBio; CAS#69-52-3); 50 μg/ml kanamycin (Kn ...
-
bioRxiv - Cancer Biology 2021Quote: ... Live-cell luciferase readings were performed for 3 consecutive days by adding 150 μg/mL luciferine (GOLDBIO, Catalog No. LUCK-500) and measuring luminescence in a Tecan Infinite F500 reader (Tecan).
-
bioRxiv - Plant Biology 2020Quote: ... 200 mM IAA in ETOH was first diluted in sterile water and then added to hydroponically growing rice roots approximately 1 day after germination to create a final concentration of 200 nM (GoldBio, I-110-25) ...
-
bioRxiv - Genomics 2021Quote: ... and then stained with the indicated antibody in FACS buffer (PBS with 1% FBS, 10 U/ml DNAse I, GoldBio, St. Louis MO) for 30 minutes on ice with gentle agitation ...
-
bioRxiv - Biochemistry 2020Quote: ... and His SUMO tags were cleaved with Ulp1 protease overnight at 4°C in Buffer C (25mM Tris-HCl pH 8.5, 150mM KCl, 2mM TCEP (GoldBio), 50mM Imidazole ...
-
bioRxiv - Plant Biology 2021Quote: ... The GUS staining buffer was then supplemented with fresh 2 mM X-Gluc (GoldBio, MO) and vacuum infiltrated again ...
-
bioRxiv - Genomics 2021Quote: ... Nanoluciferase activity was evaluated from the conditioned medium using a non-lytic 2× coelenterazine (Goldbio) reagent as previously described (91) ...
-
bioRxiv - Neuroscience 2022Quote: ... followed by a second elution with 45 µl 2% n-β-dodecylmaltoside (DDM) (Gold Biotechnologies) for 5 minutes at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... 5mM glycerol 2-phosphate and 1X ProBlock Gold Protease Inhibitor Cocktail (GoldBio, St. Louis, MO), and cooled to 4°C before use ...
-
bioRxiv - Physiology 2020Quote: ... in Krebs-Ringer-HEPES (KRH; pH 7.4) buffer containing 3% fatty acid-free bovine serum albumin (BSA; Gold Biotechnology, St. Louis, NJ), 1 g/L glucose ...
-
bioRxiv - Bioengineering 2023Quote: ... Images were acquired 10 min after intraperitoneal injection of 3 mg d-Luciferin Potassium Salt (Gold Biotechnology, Inc., St Louis MO, USA) in 200 μL 0.1 M phosphate buffered saline ...