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Citations for Gold Biotechnology :
351 - 400 of 444 citations for 7 Benzyl 4 chloro 5 6 7 8 tetrahydropyrido 3 4 d pyrimidin 2 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... in a sealed petri dish containing air-buffered bioluminescence medium (Yamazaki and Takahashi, 2005) plus D-luciferin (100 μM) (Gold Biotechnology) and incubated at 32 °C as previously described (van der Vinne et al. ...
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bioRxiv - Physiology 2021Quote: ... sections containing SCN (150 μm) were cultured on a Millicell membrane in air-buffered media containing 100 μM D-luciferin (Gold Biotechnology) and imaged for 5 days using a Stanford Photonics XR/MEGA-10Z cooled intensified charge-coupled device camera.
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bioRxiv - Cancer Biology 2022Quote: ... Luciferase expression was checked by using in vitro bioluminescence imaging by adding 100 μL of 15 mg mL-1 D-luciferin solution (#LUCNA-1G, Goldbio, USA) to the cells and imaged after 2 min using a Fusion FX system (Vilber ...
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bioRxiv - Neuroscience 2023Quote: ... Injected postnatal day one old mice were returned to the dam and after one week injected intraperitoneally with 150mg/kg firefly D-luciferin (sterile filtered 15mg/ml in phosphate buffered saline, Gold Biotechnology) (48 ...
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bioRxiv - Cancer Biology 2023Quote: ... Luc2 and NLuc expression was confirmed by exposing cells seeded in the wells of a flat-bottom white 96-well plate to 1 volume of 15 mg/mL D-luciferin (#LUCNA-1G, Goldbio, USA) or 1 volume of 0.5 mg/mL Nano-Glo In Vivo Substrate ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were grown at 37 °C to an OD600 of 0.8 and then induced using 1 mM isopropyl-β-d-1-thiogalactopyranoside (IPTG; GoldBio, 2481C) for 8 h at 30 °C ...
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bioRxiv - Microbiology 2023Quote: ... CYE was supplemented with 10 µg mL-1 chloramphenicol for plasmid maintenance and legC4 expression was induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG; GoldBio) where indicated ...
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bioRxiv - Biochemistry 2024Quote: ... media in the wells was replaced with the assay buffer (20mM HEPES pH 7.4, 1X Hank’s Balanced Salt Solution/ HBSS and 0.5mg/mL D-luciferin (GoldBio, Cat. no: LUCNA-1G). Luminescence was recorded immediately in a microplate reader (BMG Labtech) ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in assay buffer (20mM HEPES pH 7.4, 1X Hank’s Balanced Salt Solution/ HBSS and 0.5mg/mL D-luciferin (GoldBio, Cat. no: LUCNA-1G) and seeded in 96-well plates at a density of 100,000 cells/well ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor volume was monitored through bioluminescence using IVIS Spectrum apparatus (Caliper Life Sciences) after i.p injection of D-luciferin 150mg/Kg (GOLDBIO #LUCK-1G). Mice were sacrificed when manifested more than 20% of body weight loss or neurological symptoms ...
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bioRxiv - Neuroscience 2023Quote: ... Bacteria were grown to an OD600 of 0.6 and induced with 500 µM isopropyl β-D-1-thioga-lactopyranoside (IPTG) (GoldBio, I2481C) for 18 h at 18°C ...
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bioRxiv - Cell Biology 2021Quote: ... a solution of 2% low-melt agarose (Gold Biotechnology, A-204) was made in L-15 media (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... selection was performed with 2 μg/mL puromycin (GoldBio, P-600). The protocol for generating ATG3 KO Hela cells stably expressing HaloTag-LC3B is available at dx.doi.org/10.17504/protocols.io.5qpvo3xq9v4o/v1.
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in 2 % BSA (A-420-500 GoldBio) in TBS-T to the indicated concentration and incubated with membranes overnight at 4C with rocking ...
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bioRxiv - Biochemistry 2019Quote: ... wells were washed 3x with Buffer A and blocked with 5% BSA (GoldBio) in HBS with 2 mM Mg2+ ...
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bioRxiv - Physiology 2023Quote: ... 5 mL of MS-EGTA buffer with 0.2% BSA (Goldbio #A-421-250) is added to the tube to stop the trypsin reaction ...
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bioRxiv - Physiology 2023Quote: ... 5 mL of MS-EGTA buffer with 0.2% BSA (Goldbio #A-421-250) is added to the tube to stop the trypsin reaction ...
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bioRxiv - Biochemistry 2022Quote: ... cells were detached from the plates, resuspended in assay buffer (1XHBSS, 20 mM HEPES, pH7.4) containing D-luciferin (0.5 mg ml-1, GoldBio, Cat. no. LUCNA-1G) and seeded into 96 well white plates (Corning ...
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bioRxiv - Microbiology 2020Quote: ... ATP-free or ATP-containing (150 μM ATP) substrate solutions were prepared containing 150 μg/mL D-Luciferin (Goldbio, St. Louis, MO), 100 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2020Quote: ... the culture was expanded 1:100 up to 2L cultures in Luria broth and induced at A600 ∼0.60 with 0.5 mM isopropyl β-D-thiogalactopyranoside (GoldBio, St. Louis MO) for 24 h at 16°C ...
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bioRxiv - Cell Biology 2021Quote: NSG mice transplanted with GAPDHLuciferase/wt and INSGFP/wt double reporter hESCs lines were i.p injected with 150mg/kg body weight of D-luciferin potassium salt (Gold Biotechnology, luck-2G) in PBS 15 minutes before imaging on a IVIS spectrum optical imaging system (PerkinElmer) ...
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bioRxiv - Cell Biology 2023Quote: ... were spotted onto LB plates supplemented with 100 µg/mL ampicillin with or without 1 mM isopropyl β-D-1-thiogalacopyranoside (IPTG, Gold Biotechnology). After drying ...
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bioRxiv - Biophysics 2023Quote: ... resuspended in the assay buffer (20mM HEPES pH 7.4 and 1X HBSS) containing 0.5mg ml-1 of D-luciferin (GoldBio, Cat. no: LUCNA-1G) and seeded in 96-well white plates (SPL Life Sciences ...
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bioRxiv - Cell Biology 2023Quote: PIP Strips (Echelon) were blocked overnight in PBS-T with 3% fatty acid-free bovine serum albumin (GoldBio) at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Ampicillin sodium (cat #A-301-5) was purchased from Gold Biotechnology (St. Louis, MO).
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bioRxiv - Cell Biology 2021Quote: ... Successful integration was positively selected for using G418 (GoldBio Cat no. G-418-5); animals retaining an extrachromosomal array were killed by heat-shock (2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... then stimulated with the coelenterazine 400A substrate (5 mM) (Gold Biotechnology #C-320-1) and read for 30 min on Mithras2 Plate Reader (Berthold ...
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bioRxiv - Neuroscience 2021Quote: ... containing phosphatase and protease inhibitor cocktail (Goldbio Cat. No. GB-108-2). Western blotting was performed using 40 μg protein/well as previously described (Mohammed et al ...
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bioRxiv - Biochemistry 2020Quote: Simple Stop™ 2 Phosphatase Inhibitor Cocktail (Gold Biotechnology, Cat. # GB-451)
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bioRxiv - Biochemistry 2022Quote: ... The digested protein was passed over a 2-mL Ni-HTC (GoldBio) gravity column to separate the tag-free NnlA ...
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bioRxiv - Biochemistry 2022Quote: Tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl, Gold Biotechnology cat. no. TCEP1)
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bioRxiv - Cancer Biology 2024Quote: ... Tumor progression was evaluated by luminescence emission on a Xenogen IVIS spectrum after intraperitoneal injection of D-luciferin (Gold Biotechnology, St. Louis, MO) injection according to the manufacturer’s directions ...
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bioRxiv - Synthetic Biology 2021Quote: ... Single colonies were inoculated into 3 mL of rich M9- glycerol media with kanamycin (34 µg/mL, Gold Biotechnology) and ampicillin (100 µg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... the clarified supernatant containing RPA4AZP was applied to a 3 mL pre-equilibrated Ni2+-NTA (Gold Biotechnology Inc., USA) gravity column ...
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bioRxiv - Cancer Biology 2024Quote: ... Blots were blocked for 1 h at RT in 1X TBST + 3% BSA Fraction V (Gold Biotechnology, Olivette, MO) and then incubated in primary antibody diluted in blocking buffer with overnight rocking at 4°C (phosphorylated and total EGFR ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were co-cultured overnight and luminescence was measured the next morning after addition of 150 μg/ml D-luciferin (Gold Biotechnology Cat# LUCNA-2G) and subsequent incubation for 5 mins at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... The virus-serum mix was subsequently added to Vero cells for 48 h before 50 nmoles of D-Luciferin (GoldBio, St Lous, MO, USA) was added to measure luminescence ...
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bioRxiv - Cell Biology 2022Quote: ... Antibiotics used in this study include kanamycin (GoldBio #K-120-5, 50 μg mL-1), and rifampicin (GoldBio #R-120-1 ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were applied to GST agarose column (Glutathione agarose resin, GoldBio Cat# G-250-5) and washed 3 times in wash buffer 1x TBS + 1mM DTT) ...
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bioRxiv - Molecular Biology 2021Quote: ... strains were selected on yeast peptone 2% dextrose (YPD) plates containing nourseothricin (GoldBio), hygromycin B (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... The supernatants were applied to a 2 ml nickel agarose (high density, GoldBio) for binding for 1 hour ...
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bioRxiv - Cell Biology 2019Quote: ... after a 3 minutes pre-incubation with of 2.5 µM coelenterazine H (BRET1 for V2R β-arrestin interaction) from Goldbio or coelenterazine 400a (BRET2 for CAAX and FYVE assays ...
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bioRxiv - Biophysics 2022Quote: ... Tris-(carboxyethyl) phosphine hydrochloride (TCEP) and adenosine-5’-triphosphate (ATP) disodium trihydrate were purchased from GoldBio. Imidazole was purchased from Acros Organics ...
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bioRxiv - Cancer Biology 2021Quote: ... the medium was supplemented with 100 μg/ml hygromycin (#H-270-5; Goldbio, St Louis MO). For serum stimulation ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% (w/v) bovine serum albumin (BSA; Gold Biotechnology, A-420-100) in tris-buffered saline (TBS ...
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bioRxiv - Neuroscience 2023Quote: ... containing ProBlock Gold Extra Strength Protease Inhibitor Cocktail Kit (GoldBio, Cat. #: GB-116-2). The cortical lysate was then aliquoted into a 96 well plate and stored at −80°C.
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bioRxiv - Bioengineering 2019Quote: ... the following antibiotic concentrations are used (both plates and in culture): 100 μg/ml ampicillin (Ap, GoldBio; CAS#69-52-3); 50 μg/ml kanamycin (Kn ...
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bioRxiv - Cancer Biology 2021Quote: ... Live-cell luciferase readings were performed for 3 consecutive days by adding 150 μg/mL luciferine (GOLDBIO, Catalog No. LUCK-500) and measuring luminescence in a Tecan Infinite F500 reader (Tecan).
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bioRxiv - Physiology 2022Quote: ... N2 worms were egg-prepped onto solid NGM with 50 μg/ml carbenicillin (GoldBio, C-103-5) and HT115(DE3 ...
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bioRxiv - Immunology 2020Quote: ... Clarified cell supernatant containing recombinant antibodies was passed over Protein A Agarose (Goldbio Cat# P-400-5), followed by extensive washing with PBS ...