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Citations for Gold Biotechnology :
251 - 300 of 302 citations for 6 nitrobenzo d isoxazol 3 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Bacteria grew at 37°C and protein induction occurred at 20°C for overnight upon addition of 0.1mM isopropyl D-1-thigalactopyranoside (IPTG; Goldbio, I2481C) to the bacterial culture at mid-log phase (OD600nm=0.6) ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were anesthetized and injected retro-orbitally with 1.5 mg (75 mg/kg) of D-luciferin (Gold Biotechnology; LUCK-1G) at the indicated times after injecting cells ...
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bioRxiv - Biochemistry 2024Quote: ... Whole leaves were removed after 4 days of incubation and were sprayed with a solution containing 1 mM D-luciferin (GoldBio) and 0.01% Silwett L-77 (Lehle Seeds Inc ...
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bioRxiv - Biochemistry 2024Quote: ... Two-hundred μl of cells were mixed with 200 μl of 5 mM D-luciferin K+ salt (GoldBio, LUCK-100) diluted in Tyrode’s buffer and incubated at 28 °C for 15 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... the mice were injected intraperitoneally with 10 μl/g of 15 mg/mL D-luciferin potassium salt solution (Gold Biotechnology) and imaged using the Xenogen IVIS 200 In Vivo Imaging System (PerkinElmer) ...
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bioRxiv - Microbiology 2024Quote: ... Transgenic parasites were selected based on plasmid resistance (blasticidin-S-deaminase) to 6 µM blasticidin-S (Gold Biotechnology B-800-100). Transgenic parasites modified with the aptamer/TetR-DOZI system or pTEOE episomes were cultured in 0.5-1 µM anhydrotetracycline (aTc ...
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bioRxiv - Cancer Biology 2022Quote: ... Bioluminescence signals from luciferase-expressing cells in live mice were recorded 10 minutes after intraperitoneal injection of D-luciferin (80 mg/kg) (Gold Biotechnology) with IVIS Lumina III Imaging System (PerkinElmer) ...
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bioRxiv - Biophysics 2019Quote: ... at which point protein expression was induced by the addition of 0.5 mM isopropyl 1-thio-β-D-galactopyranoside (Gold Biotechnology). Temperature was reduced to 16°C for overnight expression ...
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bioRxiv - Biophysics 2020Quote: ... 1% of primary culture was inoculated in 750 mL of LB media and induced with 0.3 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Gold Biotechnology) at OD600 ~ 0.6 and further incubated at 16 °C for 14–16 h with constant shaking at 200 rpm ...
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bioRxiv - Biochemistry 2021Quote: ... expression cultures were grown at 37 °C to an optical density (OD600) of 3.0 before induction with 500 μM isopropyl-β-D-1-thiogalactopyranoside (IPTG; Gold Biotechnology) and incubation overnight at 16 °C ...
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bioRxiv - Physiology 2021Quote: ... in a sealed petri dish containing air-buffered bioluminescence medium (Yamazaki and Takahashi, 2005) plus D-luciferin (100 μM) (Gold Biotechnology) and incubated at 32 °C as previously described (van der Vinne et al. ...
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bioRxiv - Physiology 2021Quote: ... sections containing SCN (150 μm) were cultured on a Millicell membrane in air-buffered media containing 100 μM D-luciferin (Gold Biotechnology) and imaged for 5 days using a Stanford Photonics XR/MEGA-10Z cooled intensified charge-coupled device camera.
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bioRxiv - Cancer Biology 2022Quote: ... recipe of FACS buffer was RPMI-1640 + 5% FBS + 2% BSA + 50 µg/ml DNase-I (Gold Biotechnology, D-301-500). For all other primary cell staining ...
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bioRxiv - Cancer Biology 2022Quote: ... Luciferase expression was checked by using in vitro bioluminescence imaging by adding 100 μL of 15 mg mL-1 D-luciferin solution (#LUCNA-1G, Goldbio, USA) to the cells and imaged after 2 min using a Fusion FX system (Vilber ...
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bioRxiv - Neuroscience 2023Quote: ... Injected postnatal day one old mice were returned to the dam and after one week injected intraperitoneally with 150mg/kg firefly D-luciferin (sterile filtered 15mg/ml in phosphate buffered saline, Gold Biotechnology) (48 ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were grown at 37 °C to an OD600 of 0.8 and then induced using 1 mM isopropyl-β-d-1-thiogalactopyranoside (IPTG; GoldBio, 2481C) for 8 h at 30 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Luc2 and NLuc expression was confirmed by exposing cells seeded in the wells of a flat-bottom white 96-well plate to 1 volume of 15 mg/mL D-luciferin (#LUCNA-1G, Goldbio, USA) or 1 volume of 0.5 mg/mL Nano-Glo In Vivo Substrate ...
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bioRxiv - Microbiology 2023Quote: ... CYE was supplemented with 10 µg mL-1 chloramphenicol for plasmid maintenance and legC4 expression was induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG; GoldBio) where indicated ...
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bioRxiv - Cell Biology 2023Quote: ... into a centrifuge tube and then lysed with lysis buffer (50mM Tris, pH 7.5, 150mM NaCl, and 2 mM Dodecyl-B-D-maltoside (DDM) (GoldBio, catalog #: DDM5) supplemented with complete protease inhibitor cocktail (Roche #04693116001) ...
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bioRxiv - Biochemistry 2024Quote: ... media in the wells was replaced with the assay buffer (20mM HEPES pH 7.4, 1X Hank’s Balanced Salt Solution/ HBSS and 0.5mg/mL D-luciferin (GoldBio, Cat. no: LUCNA-1G). Luminescence was recorded immediately in a microplate reader (BMG Labtech) ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in assay buffer (20mM HEPES pH 7.4, 1X Hank’s Balanced Salt Solution/ HBSS and 0.5mg/mL D-luciferin (GoldBio, Cat. no: LUCNA-1G) and seeded in 96-well plates at a density of 100,000 cells/well ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor volume was monitored through bioluminescence using IVIS Spectrum apparatus (Caliper Life Sciences) after i.p injection of D-luciferin 150mg/Kg (GOLDBIO #LUCK-1G). Mice were sacrificed when manifested more than 20% of body weight loss or neurological symptoms ...
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bioRxiv - Neuroscience 2023Quote: ... Bacteria were grown to an OD600 of 0.6 and induced with 500 µM isopropyl β-D-1-thioga-lactopyranoside (IPTG) (GoldBio, I2481C) for 18 h at 18°C ...
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bioRxiv - Biochemistry 2022Quote: ... cells were detached from the plates, resuspended in assay buffer (1XHBSS, 20 mM HEPES, pH7.4) containing D-luciferin (0.5 mg ml-1, GoldBio, Cat. no. LUCNA-1G) and seeded into 96 well white plates (Corning ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed with lysis buffer (50 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM n-Dodecyl-B-D-maltoside (DDM) (GoldBio, catalog #: DDM-5)) supplemented with protease inhibitor cocktail (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... ATP-free or ATP-containing (150 μM ATP) substrate solutions were prepared containing 150 μg/mL D-Luciferin (Goldbio, St. Louis, MO), 100 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2020Quote: ... the culture was expanded 1:100 up to 2L cultures in Luria broth and induced at A600 ∼0.60 with 0.5 mM isopropyl β-D-thiogalactopyranoside (GoldBio, St. Louis MO) for 24 h at 16°C ...
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bioRxiv - Cell Biology 2021Quote: NSG mice transplanted with GAPDHLuciferase/wt and INSGFP/wt double reporter hESCs lines were i.p injected with 150mg/kg body weight of D-luciferin potassium salt (Gold Biotechnology, luck-2G) in PBS 15 minutes before imaging on a IVIS spectrum optical imaging system (PerkinElmer) ...
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bioRxiv - Biochemistry 2023Quote: ... 20 mM of 4-(2- hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) pH 7.4 and D-luciferin (0.5 mg ml-1) (GoldBio, Cat. no.: LUCNA-1G). Harvested cells were then seeded in an opaque flat bottom white 96 well cell culture plate (SPL life sciences ...
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bioRxiv - Cell Biology 2023Quote: ... were spotted onto LB plates supplemented with 100 µg/mL ampicillin with or without 1 mM isopropyl β-D-1-thiogalacopyranoside (IPTG, Gold Biotechnology). After drying ...
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bioRxiv - Biophysics 2023Quote: ... resuspended in the assay buffer (20mM HEPES pH 7.4 and 1X HBSS) containing 0.5mg ml-1 of D-luciferin (GoldBio, Cat. no: LUCNA-1G) and seeded in 96-well white plates (SPL Life Sciences ...
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bioRxiv - Cell Biology 2023Quote: Cells were harvested and lysed with lysis buffer (50 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM n-Dodecyl-β-D-maltoside (DDM) (GoldBio, catalog #: DDM5)) supplemented with complete protease inhibitor cocktail (Roche ...
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bioRxiv - Cell Biology 2023Quote: PIP Strips (Echelon) were blocked overnight in PBS-T with 3% fatty acid-free bovine serum albumin (GoldBio) at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Fecal samples were collected regularly over 2-6 weeks and plated onto SAB and SAB with nourseothricin (100 μg/mL, Gold Biotechnology, N-500-1) plates.
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bioRxiv - Cancer Biology 2022Quote: ... Spectral bioluminescent images of live cells growing in vitro were acquired 2 minutes after adding 150 ug/ml of D-Luciferin (Goldbio, Saint Louis, MO). Sequential images were acquired through optical filters ranging from 520 to 660nm at 20nm intervals ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor progression was evaluated by luminescence emission on a Xenogen IVIS spectrum after intraperitoneal injection of D-luciferin (Gold Biotechnology, St. Louis, MO) injection according to the manufacturer’s directions ...
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bioRxiv - Synthetic Biology 2021Quote: ... Single colonies were inoculated into 3 mL of rich M9- glycerol media with kanamycin (34 µg/mL, Gold Biotechnology) and ampicillin (100 µg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... the clarified supernatant containing RPA4AZP was applied to a 3 mL pre-equilibrated Ni2+-NTA (Gold Biotechnology Inc., USA) gravity column ...
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bioRxiv - Cancer Biology 2024Quote: ... Blots were blocked for 1 h at RT in 1X TBST + 3% BSA Fraction V (Gold Biotechnology, Olivette, MO) and then incubated in primary antibody diluted in blocking buffer with overnight rocking at 4°C (phosphorylated and total EGFR ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were co-cultured overnight and luminescence was measured the next morning after addition of 150 μg/ml D-luciferin (Gold Biotechnology Cat# LUCNA-2G) and subsequent incubation for 5 mins at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... The virus-serum mix was subsequently added to Vero cells for 48 h before 50 nmoles of D-Luciferin (GoldBio, St Lous, MO, USA) was added to measure luminescence ...
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bioRxiv - Cell Biology 2019Quote: ... after a 3 minutes pre-incubation with of 2.5 µM coelenterazine H (BRET1 for V2R β-arrestin interaction) from Goldbio or coelenterazine 400a (BRET2 for CAAX and FYVE assays ...
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bioRxiv - Bioengineering 2019Quote: ... the following antibiotic concentrations are used (both plates and in culture): 100 μg/ml ampicillin (Ap, GoldBio; CAS#69-52-3); 50 μg/ml kanamycin (Kn ...
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bioRxiv - Cancer Biology 2021Quote: ... Live-cell luciferase readings were performed for 3 consecutive days by adding 150 μg/mL luciferine (GOLDBIO, Catalog No. LUCK-500) and measuring luminescence in a Tecan Infinite F500 reader (Tecan).
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bioRxiv - Cancer Biology 2020Quote: ... Mice were shaved front and back and Nair was applied to remove remainder of the hair before being IP injected with D-luciferin (Goldbio LUCK-1G, 150mg/kg in 150 mL). Mice are placed in a supine position on the light-tight chamber of the CCD camera imaging unit ...
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bioRxiv - Physiology 2020Quote: ... in Krebs-Ringer-HEPES (KRH; pH 7.4) buffer containing 3% fatty acid-free bovine serum albumin (BSA; Gold Biotechnology, St. Louis, NJ), 1 g/L glucose ...
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bioRxiv - Molecular Biology 2023Quote: ... in Krebs-Ringer-HEPES (KRH; pH 7.4) buffer containing 3% fatty acid-free bovine serum albumin (BSA; Gold Biotechnology, St. Louis, NJ), 1 g/L glucose ...
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bioRxiv - Cancer Biology 2020Quote: The cytokine used for Ba/F3 culturing was 10 ng/mL murine IL-3 (Cat.#31310-03-10; Gold Biotechnology, St Louis, US).
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bioRxiv - Genetics 2021Quote: ... After extraction for 30 min at RT samples were centrifuged 600g 3 min and supernatants were precleared with 15μl of protein A agarose beads (Goldbio, cat. P-400-5) for 2 hours at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... the following were added directly to worm plates 3 days after injection: HygR selection −100 ul of 20 mg/ml hygromycin B (GoldBio, St. Louis, MO), NeoR selection - 500 ul of 25 mg/ml G418 disulfate (Sigma ...