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Citations for Gold Biotechnology :
101 - 146 of 146 citations for 6 Chloro 3 2 chloroethyl 1H pyrrolo 2 3 b pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: The cytokine used for Ba/F3 culturing was 10 ng/mL murine IL-3 (Cat.#31310-03-10; Gold Biotechnology, St Louis, US).
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bioRxiv - Genetics 2021Quote: ... After extraction for 30 min at RT samples were centrifuged 600g 3 min and supernatants were precleared with 15μl of protein A agarose beads (Goldbio, cat. P-400-5) for 2 hours at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Mice were imaged on the IVIS every 3-4 days after being sedated with Isoflurane and intraperitoneal injections of ∼120 mg/kg D-luciferin (GoldBio, St. Louis, MI). Mice were injected with 100,000 IU of human IL-2 (National Cancer Institute ...
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bioRxiv - Biochemistry 2022Quote: ... in the medium with the exception of the 293T-based CAPN5-3×FLAG stable cell line that was selected and maintained with 200 µg/ml hygromycin (Gold Biotechnology, H-270-1).
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bioRxiv - Synthetic Biology 2023Quote: ... CAS NO 3483-12-3), isopropyl-β-thiogalactopyranoside (IPTG, CAS NO 367-93-1) and bovine serum albumin (BSA) were obtained from Gold Biotechnology (Burlington, MA). Alexa Fluor 594 C5 maleimide was purchased from BroadPharm (San Diego ...
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bioRxiv - Microbiology 2022Quote: ... selected with 5 μg/ml blasticidin (GoldBio #B-800-25), and subcloned by serial dilution to obtain single cell clones ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were selected in 200µg/mL hygromycin B (Gold Biotechnology), colonies isolated with trypsin-EDTA-soaked filter papers and expanded ...
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bioRxiv - Biochemistry 2021Quote: ... All lysis buffers used were supplemented with 1X protease inhibitor cocktail11 and 2 mM AEBSF (Gold Biotech). A list of constructs used can be found in Supplementary Table 2.
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bioRxiv - Cell Biology 2021Quote: ... 200 µl diluted cells (2 x104 cells) and 50 µl (8 mg/ml) D-luciferin (GoldBio, USA) were loaded into each well ...
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bioRxiv - Molecular Biology 2022Quote: ... 120 µg protein was mixed with substrate containing 15mM ONPG (2-Nitrophenyl-β-D-galactopyranoside, Goldbio, #N27510), 5mM DTT ...
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bioRxiv - Biophysics 2019Quote: ... 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (HEPES) and Dithiothreitol (DTT) were purchased from GoldBio (St Louis, MO, US). Ethylene Glycol-bis(β-aminoethyl ether)-N,N,N′,N′-Tetraacetic Acid (EGTA) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Inducers and antibiotics were used at the following working concentrations: 2 mg/mL L-arabinose (GoldBio A-300), 1 mM IPTG (GoldBio I2481C) ...
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bioRxiv - Physiology 2020Quote: ... were washed twice with PBS and starved of serum for 2 h in DMEM with 0.1% BSA (GoldBio). Following starvation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were washed once with 1× PBS and 75 μL of 2 mM D-luciferin (LUCK-1G, Goldbio) in Leibovitz’s L-15 medium (21083-027 ...
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bioRxiv - Bioengineering 2023Quote: ... Inducers and antibiotics were used at the following working concentrations: 2 mg/ml L-arabinose (GoldBio, A-300), 1 mM IPTG (GoldBio ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were selected using 200 μg/ml hygromycin B (Gold Biotechnology), and single colonies were isolated using trypsin-soaked filter paper ...
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bioRxiv - Molecular Biology 2023Quote: ... with all purification buffers for full-length WT NEMO also containing 5 mM Tris(2-carboxyethyl)phosphine (TCEP) (GoldBio) to minimize the formation of disulfide0bonded aggregates ...
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bioRxiv - Cancer Biology 2023Quote: ... and imaged within 2-4 minutes after retro-orbital injection of 1.5 mg D-luciferin (GoldBio, Cat: LUCK-10G) dissolved in 100 µL PBS ...
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bioRxiv - Plant Biology 2023Quote: ... 75 µL of water was replaced with 25 µL of a 2 mM Luciferin (GoldBio; Catalog number: LUCNA-100) solution ...
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bioRxiv - Microbiology 2024Quote: Mice received an IP injection of 100 μl of a 2% D-luciferin solution (Goldbio cat #115144-35-9), were anaesthetized with isoflurane gas ...
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bioRxiv - Microbiology 2020Quote: ... 2.5 Units/ml polymyxin B (all from Gold Biotechnology, St Louis, MO), and 0.2% (wt/vol ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were selected in 200 μg ml−1 Hygromycin B (Gold Biotechnology) and colonies were isolated and expanded ...
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bioRxiv - Biochemistry 2024Quote: ... and induced with 1 mM Isopropyl b-d-1-thiogalactopyranoside (IPTG, Goldbio) overnight at room temperature ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Equal volumes of each sample were loaded onto a 4-12% SDS-PAGE gel along with the BLUEstainTM 2 (GoldBio) protein ladder ...
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bioRxiv - Cancer Biology 2022Quote: ... recipe of FACS buffer was RPMI-1640 + 5% FBS + 2% BSA + 50 µg/ml DNase-I (Gold Biotechnology, D-301-500). For all other primary cell staining ...
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bioRxiv - Systems Biology 2023Quote: ... Permeabilized cells were washed twice with 1X PBS then resuspended in 0.01% Triton-X-100/2% BSA/1X PBS (GoldBio #A-420) at room temperature on a shaker for 1 hour or overnight at 4 °C to block non-specific protein binding ...
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bioRxiv - Biochemistry 2023Quote: ... Luminescence was measured for 2 s in a Lumat LB 9507 (Berthold Technologies) after injection of 100 µl 250 nM Luciferin (Gold Biotechnology). Luciferase Refolding served as a measure for disaggregation activity ...
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bioRxiv - Genetics 2022Quote: ... 60 nmol dye aliquots were made from either Cy3 (Gold Biotechnology, B-230-1) or Alexa 647 (ThermoFisher Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... which were selected for resistance to 300 μg/mL hygromycin B (GoldBio, H-270), 100 μg/mL nourseothricin sulfate (GoldBio ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with 8 ug/ml of blasticidin (Gold Biotechnology, B-800-100) for 48 h to select for cells that had integrated the Xlone-mScarlet-Myo10 plasmid ...
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bioRxiv - Synthetic Biology 2021Quote: ... and to this 0.85 mL of liquid (70 °C) top agar (2XYT media + 0.6% agar) supplemented with 2% Bluo-Gal (GoldBio, CAS #97753-82-7) was added and mixed by pipetting up and down once ...
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bioRxiv - Biochemistry 2023Quote: ... 20 mM of 4-(2- hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) pH 7.4 and D-luciferin (0.5 mg ml-1) (GoldBio, Cat. no.: LUCNA-1G). Harvested cells were then seeded in an opaque flat bottom white 96 well cell culture plate (SPL life sciences ...
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bioRxiv - Cell Biology 2023Quote: Cells were harvested and lysed with lysis buffer (50 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM n-Dodecyl-β-D-maltoside (DDM) (GoldBio, catalog #: DDM5)) supplemented with complete protease inhibitor cocktail (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... or fog-2(q71) along with approximately 30 wild type males were placed on RNAi plates [MYOB containing 1mM IPTG (GoldBio, Cat. No. I2481C) and seeded with the appropriate dsRNA expressing HT115 cells] ...
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bioRxiv - Cancer Biology 2022Quote: ... Spectral bioluminescent images of live cells growing in vitro were acquired 2 minutes after adding 150 ug/ml of D-Luciferin (Goldbio, Saint Louis, MO). Sequential images were acquired through optical filters ranging from 520 to 660nm at 20nm intervals ...
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bioRxiv - Biophysics 2023Quote: ... selection media was HCT116 growth media supplemented with 6µg/mL of blasticidin (GoldBio, B-800-25).
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bioRxiv - Microbiology 2021Quote: ... coli TOP10 and plated on LB agar plates supplemented with 100 μg/mL hygromycin B (GoldBio, USA). An individual colony was picked and transferred to 5 mL of LB medium supplemented with 100 μg/mL hygromycin B and incubated overnight at 37 °C ...
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bioRxiv - Plant Biology 2023Quote: ... The primary transformants were screened on ½ MS plates containing 15 µg ml-1 hygromycin B (Gold Biotechnology). After 2 weeks ...
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bioRxiv - Microbiology 2022Quote: ... Transduced cells were selected at 48 hours post-transduction with 8μg/mL Blasticidin S (GoldBio, #B-800-100), expanded ...
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bioRxiv - Bioengineering 2024Quote: ... at 1 mg/mL and elastase at 6 U/mL (E-240, Goldbio) for 1 h ...
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bioRxiv - Microbiology 2019Quote: ... kanamycin (Km; E.coli at 50 μg/mL or B. bronchiseptica at 125 μg/mL; Gold Biotechnology, Cat#: K-120-25), or diaminopimelic acid (DAP ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were harvested by centrifugation (6000xg for 20 min) and lysed with B-PER Bacterial Protein Extraction Reagent (Thermo Fischer) supplemented with DNAse I (GoldBio). Lysate was incubated at 60°C for 20 min and cleared by centrifugation at 14,000xg for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... and screening for loss of b-glucuronidase activity via plating on YT plates containing 50 μg/ml X-Gluc (cyclohexlammonium salt, Gold Biotechnologies, G1281C1). Strains were confirmed by PCR amplification and DNA sequencing of the region of interest.
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bioRxiv - Cancer Biology 2024Quote: ... Protein expression was induced for ∼6 h by 1 mM isopropyl β-a-D-1-thiogalactopyranoside (IPTG; GoldBio) in an 18 °C shaker ...
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bioRxiv - Cell Biology 2022Quote: KO2 cells of an early passage were infected with lentiviruses expressing HMGB2- eYFP or FOXM1-eYFP fusion proteins and selected with 20 µg/ml hygromycin B (Goldbio Biotechnology, H-270-1). Pools of cells were then cultured in the presence of hygromycin ...
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bioRxiv - Biochemistry 2020Quote: ... washed twice with PBS and once with 50 mM HEPES pH 7.6, and resuspended in lysis buffer (50 mM HEPES pH 7.6, 1% Nonidet-P40, 0.2 mM TCEP (Gold Biotechnology), and Roche cOmplete EDTA-free Protease Inhibitor Cocktail) ...