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Citations for Gold Biotechnology :
151 - 200 of 209 citations for 6 Bromo 2 methylthiazolo 5 4 b pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Bioluminescence images were taken once weekly 5 min following injection of D-luciferin (Gold Biotechnology, US) with an in vivo imaging system (IVIS Spectrum ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% (w/v) bovine serum albumin (BSA; Gold Biotechnology, A-420-100) in tris-buffered saline (TBS ...
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bioRxiv - Neuroscience 2023Quote: ... containing ProBlock Gold Extra Strength Protease Inhibitor Cocktail Kit (GoldBio, Cat. #: GB-116-2). The cortical lysate was then aliquoted into a 96 well plate and stored at −80°C.
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bioRxiv - Physiology 2022Quote: ... N2 worms were egg-prepped onto solid NGM with 50 μg/ml carbenicillin (GoldBio, C-103-5) and HT115(DE3 ...
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bioRxiv - Immunology 2020Quote: ... Clarified cell supernatant containing recombinant antibodies was passed over Protein A Agarose (Goldbio Cat# P-400-5), followed by extensive washing with PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked in 5% bovine serum albumin (BSA, #A-420-1, Gold Biotechnology, St. Louis, MO), for phospho-specific antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... and His SUMO tags were cleaved with Ulp1 protease overnight at 4°C in Buffer C (25mM Tris-HCl pH 8.5, 150mM KCl, 2mM TCEP (GoldBio), 50mM Imidazole ...
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bioRxiv - Plant Biology 2023Quote: ... Whole leaves were removed after 4 d incubation and leaves were sprayed with a 1 mM D-luciferin (GoldBio) and 0.01% Silwett L-77 (Lehle Seeds Inc ...
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bioRxiv - Plant Biology 2021Quote: ... The GUS staining buffer was then supplemented with fresh 2 mM X-Gluc (GoldBio, MO) and vacuum infiltrated again ...
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bioRxiv - Genomics 2021Quote: ... Nanoluciferase activity was evaluated from the conditioned medium using a non-lytic 2× coelenterazine (Goldbio) reagent as previously described (91) ...
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bioRxiv - Neuroscience 2022Quote: ... followed by a second elution with 45 µl 2% n-β-dodecylmaltoside (DDM) (Gold Biotechnologies) for 5 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 5mM glycerol 2-phosphate and 1X ProBlock Gold Protease Inhibitor Cocktail (GoldBio, St. Louis, MO), and cooled to 4°C before use ...
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bioRxiv - Plant Biology 2024Quote: ... seedlings were rinsed with 1/2MS with 10 µM Timentin (Gold Biotechnology, Cat# T104-2), transferred into a fresh 1/2MS medium ...
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bioRxiv - Neuroscience 2020Quote: Each Tg(Gfap-luc) animal was given 5 mg (100 μL of 50 mg/mL) D-luciferin (GoldBio) in saline by intraperitoneal injection ...
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bioRxiv - Cell Biology 2022Quote: ... the procedure was similar except the culture media also contained 5ng/ml GM-CSF (GoldBio # 1320-03-5) to stimulate microglial proliferation ...
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bioRxiv - Developmental Biology 2023Quote: ... plants were incubated with an aqueous solution of 100 µg/ml Carbenicillin (GoldBio, C-103-5, lot # 0129.091814A) for 30 minutes.
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bioRxiv - Biochemistry 2024Quote: ... Whole leaves were removed after 4 days of incubation and were sprayed with a solution containing 1 mM D-luciferin (GoldBio) and 0.01% Silwett L-77 (Lehle Seeds Inc ...
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bioRxiv - Biophysics 2021Quote: ... the GltPh proteins were reduced with 500 µM tris(2-carboxy-ethyl) phosphine (TCEP, Gold Biotechnology) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... The beads bound with biotinylated cell surface proteins were resuspended in 50 mM Tris (pH 8.5) + 4 M urea + 10 mM TCEP (Gold Biotechnology, TCEP10) and 20 mm IAA (VWR ...
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bioRxiv - Biophysics 2021Quote: ... and resuspended in assay buffer (HBSS with 20 mM HEPES pH 7.45) with 5 μg/mL freshly prepared coelenterazine 400a (GoldBio). Cells were then placed in white-walled ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were collected by centrifugation at 6,000g for 20 min and resuspended in a final volume of 40mL cell lysis buffer (100 mM Tris-HCl, pH 8.0, 1 M NaCl, 20% glycerol, 5 mM TCEP (GoldBio), and 2 cOmplete™ EDTA-free protease inhibitor cocktail tablets (Sigma)) ...
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bioRxiv - Biochemistry 2022Quote: ... then the cleared bacterial lysate was passed through a 0.22 μm PES filter (Genessee Scientific) and then applied to a 5 mL packed Ni-NTA agarose beads (Goldbio) equilibrated with Lysis buffer ...
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bioRxiv - Biochemistry 2023Quote: ... ten-fold serial dilutions were plated onto synthetic complete agar lacking histidine and containing 0.1% w/v 5-fluoroorotic acid (GoldBio). Plasmid shuffling experiments involving Use1 were conducted in an analogous manner ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were transferred onto fresh media and treated with 100 µg/ml Carbenicillin (GoldBio, C-103-5, lot # 0129.091814A).
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bioRxiv - Biophysics 2024Quote: ... Cleared lysate was loaded onto lysis buffer-equilibrated Ni-NTA resin (GoldBio, 5 mL resin per 50 mL lysate) and rocked gently for 1 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... All lysis buffers used were supplemented with 1X protease inhibitor cocktail11 and 2 mM AEBSF (Gold Biotech). A list of constructs used can be found in Supplementary Table 2.
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bioRxiv - Cell Biology 2021Quote: ... 200 µl diluted cells (2 x104 cells) and 50 µl (8 mg/ml) D-luciferin (GoldBio, USA) were loaded into each well ...
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bioRxiv - Molecular Biology 2022Quote: ... 120 µg protein was mixed with substrate containing 15mM ONPG (2-Nitrophenyl-β-D-galactopyranoside, Goldbio, #N27510), 5mM DTT ...
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bioRxiv - Molecular Biology 2021Quote: Purification of the mouse lectin-mIgG2a fusion proteins was performed using Protein A agarose resin (Gold Biotechnology, P-400-5). The protein A beads were pelleted at 150g for 5 min and washed once with 1x binding buffer (0.02 M Sodium Phosphate ...
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bioRxiv - Microbiology 2020Quote: ... The protein was eluted with 5 mL of elution buffer (20 mM TEA, 50 mM KCl, 500 mM imidazole (GoldBio), pH 7) ...
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bioRxiv - Microbiology 2020Quote: ... The protein eluted and collected in 5-1mL aliquots of elution buffer (50 mM NaH2PO4, 300 mM NaCl, and 250 mM imidazole (GoldBio) (pH 8.0)) ...
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bioRxiv - Developmental Biology 2022Quote: ... 50 μL of DNase (stock: 5 mg/mL; final concentration: 0.025 mg/mL or 50 U/ml, GoldBio, D-301), and 100 μL of Collagenase Type I (stock ...
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bioRxiv - Genetics 2023Quote: ... and depleted of non-transposed cells by resuspending the pelleted cells in 300 mL of fresh SCD media containing 1 mg/mL of 5-fluoroorotic acid (ZymoResearch) and 0.1 mg/mL of nourseothricin (Gold Biotechnology) and shaking overnight at 30°C ...
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bioRxiv - Biochemistry 2024Quote: ... Two-hundred μl of cells were mixed with 200 μl of 5 mM D-luciferin K+ salt (GoldBio, LUCK-100) diluted in Tyrode’s buffer and incubated at 28 °C for 15 minutes ...
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bioRxiv - Synthetic Biology 2022Quote: ... Inducers and antibiotics were used at the following working concentrations: 2 mg/mL L-arabinose (GoldBio A-300), 1 mM IPTG (GoldBio I2481C) ...
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bioRxiv - Physiology 2020Quote: ... were washed twice with PBS and starved of serum for 2 h in DMEM with 0.1% BSA (GoldBio). Following starvation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were washed once with 1× PBS and 75 μL of 2 mM D-luciferin (LUCK-1G, Goldbio) in Leibovitz’s L-15 medium (21083-027 ...
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bioRxiv - Bioengineering 2023Quote: ... Inducers and antibiotics were used at the following working concentrations: 2 mg/ml L-arabinose (GoldBio, A-300), 1 mM IPTG (GoldBio ...
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bioRxiv - Bioengineering 2024Quote: ... Mice were imaged on the IVIS every 3-4 days after being sedated with Isoflurane and intraperitoneal injections of ∼120 mg/kg D-luciferin (GoldBio, St. Louis, MI). Mice were injected with 100,000 IU of human IL-2 (National Cancer Institute ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were resuspended in 3 ml assay buffer (HBSS buffer with 0.01% BSA and 5 mM HEPES, pH 7.4) containing 10 μM coelenterazine (Goldbio, Cat. no: CZ05) at final concentration ...
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bioRxiv - Biochemistry 2022Quote: ... and resuspended in assay buffer (1XHBSS, 5 mM HEPES, pH 7.4, 0.01% BSA) containing 10 μM coelenterazine (GoldBio, Cat no. CZ2.5). Resuspended cells were seeded in a white flat bottom 96-well plate (100 μl well-1) ...
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bioRxiv - Molecular Biology 2023Quote: ... each plate was sprayed with 1 milliliter of an aqueous solution of 5 mM firefly luciferin (Gold Biotechnology, St Louis, MO) and 0.01% (vol/vol ...
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bioRxiv - Biochemistry 2023Quote: ... and resuspended in the NanoBiT assay buffer (HBSS containing 0.01% BSA, 5 mM HEPES, pH 7.4, and 10 μM coelenterazine (GoldBio, Cat no: CZ05). After resuspension ...
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bioRxiv - Microbiology 2023Quote: ... after completion of virus infection virus containing media was replaced with media containing 5 µg/ml MTT reagent (Thiazolyl blue tetrazolium bromide, cat no.298-93-1, Gold biotechnology). Incubate at 37°C for 3-4 hr and then remove MTT containing media and add 100 µl of DMSO to each well to dissolve the formazan crystals formed ...
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bioRxiv - Plant Biology 2023Quote: ... 75 µL of water was replaced with 25 µL of a 2 mM Luciferin (GoldBio; Catalog number: LUCNA-100) solution ...
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bioRxiv - Microbiology 2024Quote: Mice received an IP injection of 100 μl of a 2% D-luciferin solution (Goldbio cat #115144-35-9), were anaesthetized with isoflurane gas ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was filtered and then incubated with nutation at 4°C for 1 hr with Ni-NTA agarose (Gold Biotechnology #H-350-100) that was pre-equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... were transformed and plated on LB Agar (Fisher BioReagents Cat#: BP1425-500) containing 50 µg/mL carbenicillin (GoldBio Cat#: C-103-5) and incubated overnight at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... The G418-resistance cassettes were transformed into FY4 (MATa) and selected on YPD + G418 (400 µg/mL; Gold Biotechnology #G-418-5) plates ...
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bioRxiv - Genetics 2021Quote: ... After extraction for 30 min at RT samples were centrifuged 600g 3 min and supernatants were precleared with 15μl of protein A agarose beads (Goldbio, cat. P-400-5) for 2 hours at RT ...