Labshake search
Citations for Gold Biotechnology :
301 - 350 of 367 citations for 6 BROMOPYRIDO 2 3 D PYRIMIDINE 2 4 1H 3H DIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... A 1:100 dilution into 3 L of the same media was grown to OD600 ∼0.25 and induced with 0.4 mM Isopropyl β–D-1-thiogalactopyranoside (IPTG; Goldbio). Cells were harvested ...
-
bioRxiv - Immunology 2024Quote: ... Samples were treated with 1µl D-luciferin (30µg/mL) per 100µl sample volume (Gold Biotechnology, St. Louis, MO, USA) prior to imaging.
-
bioRxiv - Cell Biology 2022Quote: ... animals were grown on 6 cm NGM plates that were supplemented with 250 μl of 5 mg/ml of hygromycin B (GoldBio) in M9.
-
bioRxiv - Molecular Biology 2024Quote: ... The extract was centrifuged and 3 ml of glutathione agarose resin (GoldBio, cat# G-250) were added to 150 ml supernatant of GST-YFP-PANT ...
-
bioRxiv - Bioengineering 2023Quote: ... Human hairs were embedded in a 4% agarose block (A-204-25, GoldBio), and the block was placed in the water-filled customized container during imaging ...
-
bioRxiv - Microbiology 2020Quote: ... The mice were anesthetized in isoflurane and imaged after intraperitoneal injection of 150 mg/kg of D-luciferin (Gold Biotechnology). Bioluminescence imaging of MHV68-M3-FL infected mice was collected on the IVIS Lumina Series III (Perkin Elmer) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Mice received an intraperitoneal injection of 200 mg/kg D-luciferin firefly potassium salt diluted in PBS 15 minutes before imaging (GoldBio).
-
bioRxiv - Microbiology 2020Quote: ... Mice infected with Fluc-carrying viruses received in vivo grade D-Luciferin (15 mg/mL in sterile endotoxin-free PBS, GoldBio) (r.o. ...
-
bioRxiv - Biochemistry 2021Quote: ... cells were harvested and resuspended in assay buffer containing D-luciferin (0.5 mg ml−1, GoldBio, Cat. no. LUCNA-1G) in 1X HBSS ...
-
bioRxiv - Cell Biology 2019Quote: ... was measured at the indicated intervals by replacing 10% of media with MuSC media supplemented with 1.5mg/ml D-Luciferin (Gold Biotechnology) and recorded using an IVIS Spectrum (Perkin Elmer).
-
bioRxiv - Microbiology 2021Quote: ... Mice were each injected (i.p.) with 100 µl of 25 mg/ml D-luciferin potassium salt (GoldBio, St. Louis, MO) and imaged daily from days 3 dpi - 7 dpi with an In Vivo Imaging System (IVIS ...
-
bioRxiv - Immunology 2020Quote: ... Cells were plated at equal density and luminescence signals were quantified in the presence of 150 µg/ml D-luciferin (GoldBio) by imaging using cooled CCD optical macroscopic imaging system (IVIS Lumina Series III ...
-
bioRxiv - Developmental Biology 2022Quote: ... 50 μL of DNase (stock: 5 mg/mL; final concentration: 0.025 mg/mL or 50 U/ml, GoldBio, D-301), and 100 μL of Collagenase Type I (stock ...
-
bioRxiv - Cell Biology 2023Quote: ... and added to the cell medium to 1 μM final concentration. D-luciferin sodium salt (Cat. #LUCNA) and coelenterazine (Cat. #CZ) were purchased from GoldBio and resuspended to 100 mM in 10 mM HEPES buffer ...
-
bioRxiv - Cancer Biology 2023Quote: ... 15 minutes after subcutaneous injection of 100 μl (150 mg Luciferin/kg body weight) of D-luciferin (Goldbio LUCK-1G). After the final week ...
-
bioRxiv - Cell Biology 2023Quote: ... Bacteria grew at 37°C and protein induction occurred at 20°C for overnight upon addition of 0.1mM isopropyl D-1-thigalactopyranoside (IPTG; Goldbio, I2481C) to the bacterial culture at mid-log phase (OD600nm=0.6) ...
-
bioRxiv - Cancer Biology 2023Quote: ... mice were anesthetized and injected retro-orbitally with 1.5 mg (75 mg/kg) of D-luciferin (Gold Biotechnology; LUCK-1G) at the indicated times after injecting cells ...
-
bioRxiv - Biochemistry 2024Quote: ... Two-hundred μl of cells were mixed with 200 μl of 5 mM D-luciferin K+ salt (GoldBio, LUCK-100) diluted in Tyrode’s buffer and incubated at 28 °C for 15 minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... the mice were injected intraperitoneally with 10 μl/g of 15 mg/mL D-luciferin potassium salt solution (Gold Biotechnology) and imaged using the Xenogen IVIS 200 In Vivo Imaging System (PerkinElmer) ...
-
bioRxiv - Microbiology 2024Quote: ... Transgenic parasites were selected based on plasmid resistance (blasticidin-S-deaminase) to 6 µM blasticidin-S (Gold Biotechnology B-800-100). Transgenic parasites modified with the aptamer/TetR-DOZI system or pTEOE episomes were cultured in 0.5-1 µM anhydrotetracycline (aTc ...
-
bioRxiv - Cancer Biology 2022Quote: ... Bioluminescence signals from luciferase-expressing cells in live mice were recorded 10 minutes after intraperitoneal injection of D-luciferin (80 mg/kg) (Gold Biotechnology) with IVIS Lumina III Imaging System (PerkinElmer) ...
-
bioRxiv - Biophysics 2019Quote: ... at which point protein expression was induced by the addition of 0.5 mM isopropyl 1-thio-β-D-galactopyranoside (Gold Biotechnology). Temperature was reduced to 16°C for overnight expression ...
-
bioRxiv - Biophysics 2020Quote: ... 1% of primary culture was inoculated in 750 mL of LB media and induced with 0.3 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Gold Biotechnology) at OD600 ~ 0.6 and further incubated at 16 °C for 14–16 h with constant shaking at 200 rpm ...
-
bioRxiv - Biochemistry 2021Quote: ... expression cultures were grown at 37 °C to an optical density (OD600) of 3.0 before induction with 500 μM isopropyl-β-D-1-thiogalactopyranoside (IPTG; Gold Biotechnology) and incubation overnight at 16 °C ...
-
bioRxiv - Physiology 2021Quote: ... in a sealed petri dish containing air-buffered bioluminescence medium (Yamazaki and Takahashi, 2005) plus D-luciferin (100 μM) (Gold Biotechnology) and incubated at 32 °C as previously described (van der Vinne et al. ...
-
bioRxiv - Physiology 2021Quote: ... sections containing SCN (150 μm) were cultured on a Millicell membrane in air-buffered media containing 100 μM D-luciferin (Gold Biotechnology) and imaged for 5 days using a Stanford Photonics XR/MEGA-10Z cooled intensified charge-coupled device camera.
-
bioRxiv - Cancer Biology 2022Quote: ... Luciferase expression was checked by using in vitro bioluminescence imaging by adding 100 μL of 15 mg mL-1 D-luciferin solution (#LUCNA-1G, Goldbio, USA) to the cells and imaged after 2 min using a Fusion FX system (Vilber ...
-
bioRxiv - Neuroscience 2023Quote: ... Injected postnatal day one old mice were returned to the dam and after one week injected intraperitoneally with 150mg/kg firefly D-luciferin (sterile filtered 15mg/ml in phosphate buffered saline, Gold Biotechnology) (48 ...
-
bioRxiv - Neuroscience 2023Quote: ... The cells were grown at 37 °C to an OD600 of 0.8 and then induced using 1 mM isopropyl-β-d-1-thiogalactopyranoside (IPTG; GoldBio, 2481C) for 8 h at 30 °C ...
-
bioRxiv - Cancer Biology 2023Quote: ... Luc2 and NLuc expression was confirmed by exposing cells seeded in the wells of a flat-bottom white 96-well plate to 1 volume of 15 mg/mL D-luciferin (#LUCNA-1G, Goldbio, USA) or 1 volume of 0.5 mg/mL Nano-Glo In Vivo Substrate ...
-
bioRxiv - Microbiology 2023Quote: ... CYE was supplemented with 10 µg mL-1 chloramphenicol for plasmid maintenance and legC4 expression was induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG; GoldBio) where indicated ...
-
bioRxiv - Biochemistry 2024Quote: ... media in the wells was replaced with the assay buffer (20mM HEPES pH 7.4, 1X Hank’s Balanced Salt Solution/ HBSS and 0.5mg/mL D-luciferin (GoldBio, Cat. no: LUCNA-1G). Luminescence was recorded immediately in a microplate reader (BMG Labtech) ...
-
bioRxiv - Biochemistry 2024Quote: ... resuspended in assay buffer (20mM HEPES pH 7.4, 1X Hank’s Balanced Salt Solution/ HBSS and 0.5mg/mL D-luciferin (GoldBio, Cat. no: LUCNA-1G) and seeded in 96-well plates at a density of 100,000 cells/well ...
-
bioRxiv - Cancer Biology 2024Quote: ... Tumor volume was monitored through bioluminescence using IVIS Spectrum apparatus (Caliper Life Sciences) after i.p injection of D-luciferin 150mg/Kg (GOLDBIO #LUCK-1G). Mice were sacrificed when manifested more than 20% of body weight loss or neurological symptoms ...
-
bioRxiv - Neuroscience 2023Quote: ... Bacteria were grown to an OD600 of 0.6 and induced with 500 µM isopropyl β-D-1-thioga-lactopyranoside (IPTG) (GoldBio, I2481C) for 18 h at 18°C ...
-
bioRxiv - Biochemistry 2022Quote: ... cells were detached from the plates, resuspended in assay buffer (1XHBSS, 20 mM HEPES, pH7.4) containing D-luciferin (0.5 mg ml-1, GoldBio, Cat. no. LUCNA-1G) and seeded into 96 well white plates (Corning ...
-
bioRxiv - Microbiology 2020Quote: ... ATP-free or ATP-containing (150 μM ATP) substrate solutions were prepared containing 150 μg/mL D-Luciferin (Goldbio, St. Louis, MO), 100 mM Tris pH 8 ...
-
bioRxiv - Biochemistry 2020Quote: ... the culture was expanded 1:100 up to 2L cultures in Luria broth and induced at A600 ∼0.60 with 0.5 mM isopropyl β-D-thiogalactopyranoside (GoldBio, St. Louis MO) for 24 h at 16°C ...
-
bioRxiv - Cell Biology 2021Quote: NSG mice transplanted with GAPDHLuciferase/wt and INSGFP/wt double reporter hESCs lines were i.p injected with 150mg/kg body weight of D-luciferin potassium salt (Gold Biotechnology, luck-2G) in PBS 15 minutes before imaging on a IVIS spectrum optical imaging system (PerkinElmer) ...
-
bioRxiv - Cell Biology 2023Quote: ... were spotted onto LB plates supplemented with 100 µg/mL ampicillin with or without 1 mM isopropyl β-D-1-thiogalacopyranoside (IPTG, Gold Biotechnology). After drying ...
-
bioRxiv - Biophysics 2023Quote: ... resuspended in the assay buffer (20mM HEPES pH 7.4 and 1X HBSS) containing 0.5mg ml-1 of D-luciferin (GoldBio, Cat. no: LUCNA-1G) and seeded in 96-well white plates (SPL Life Sciences ...
-
bioRxiv - Cell Biology 2023Quote: PIP Strips (Echelon) were blocked overnight in PBS-T with 3% fatty acid-free bovine serum albumin (GoldBio) at 4°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... Tumor progression was evaluated by luminescence emission on a Xenogen IVIS spectrum after intraperitoneal injection of D-luciferin (Gold Biotechnology, St. Louis, MO) injection according to the manufacturer’s directions ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Single colonies were inoculated into 3 mL of rich M9- glycerol media with kanamycin (34 µg/mL, Gold Biotechnology) and ampicillin (100 µg/mL ...
-
bioRxiv - Biochemistry 2023Quote: ... the clarified supernatant containing RPA4AZP was applied to a 3 mL pre-equilibrated Ni2+-NTA (Gold Biotechnology Inc., USA) gravity column ...
-
bioRxiv - Cancer Biology 2024Quote: ... Blots were blocked for 1 h at RT in 1X TBST + 3% BSA Fraction V (Gold Biotechnology, Olivette, MO) and then incubated in primary antibody diluted in blocking buffer with overnight rocking at 4°C (phosphorylated and total EGFR ...
-
bioRxiv - Bioengineering 2022Quote: ... Cells were co-cultured overnight and luminescence was measured the next morning after addition of 150 μg/ml D-luciferin (Gold Biotechnology Cat# LUCNA-2G) and subsequent incubation for 5 mins at 37°C ...
-
bioRxiv - Microbiology 2022Quote: ... The virus-serum mix was subsequently added to Vero cells for 48 h before 50 nmoles of D-Luciferin (GoldBio, St Lous, MO, USA) was added to measure luminescence ...
-
bioRxiv - Genetics 2022Quote: ... 500 μl of 4 mg/ml stock of Hygromycin solution (Gold Biotechnology, catalog no. H-270-1) was topically added to the bacterial lawn of injection plates to select for transgenic (F1 ...
-
bioRxiv - Microbiology 2023Quote: ... Lysates were clarified via centrifugation and applied to 4 mL of pre-equilibrated Ni+-NTA resin (GoldBio) in a gravity flow column ...