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Citations for Gold Biotechnology :
251 - 300 of 308 citations for Mouse Anti Dengue Virus Envelope Protein Serotypes 1 3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... followed by the addition of 500 mM IPTG (β-D-1-thiogalactopyranoside, Gold Biotechnology, Catalog #I2481C25) for protein expression and incubation at 18°C for 15-18 hrs ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked in 5% bovine serum albumin (BSA, #A-420-1, Gold Biotechnology, St. Louis, MO), for phospho-specific antibodies ...
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bioRxiv - Genetics 2022Quote: ... 500 μl of 4 mg/ml stock of Hygromycin solution (Gold Biotechnology, catalog no. H-270-1) was topically added to the bacterial lawn of injection plates to select for transgenic (F1 ...
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bioRxiv - Plant Biology 2023Quote: ... Whole leaves were removed after 4 d incubation and sprayed with a 1 mM D-Luciferin (GoldBio) and 0.01% Silwet L-77 (Lehle Seeds Inc ...
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bioRxiv - Plant Biology 2023Quote: ... The primary transformants were screened on ½ MS plates containing 15 µg ml-1 hygromycin B (Gold Biotechnology). After 2 weeks ...
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bioRxiv - Cell Biology 2024Quote: ... Molecular mass standards for gels were 100 bp and 1 kb DNA ladders (GoldBio, St. Louis, MO).
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bioRxiv - Cell Biology 2020Quote: ... bacterial cells were grown at 37°C in Lucia Broth medium and induced at A600 nm = 0.6-0.7 by the addition of 0.1 mM isopropyl-1-thio-□-D-galactopyranoside (Gold Biotechnology) and harvested after 18h at 18°C ...
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bioRxiv - Biophysics 2019Quote: ... 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (HEPES) and Dithiothreitol (DTT) were purchased from GoldBio (St Louis, MO, US). Ethylene Glycol-bis(β-aminoethyl ether)-N,N,N′,N′-Tetraacetic Acid (EGTA) ...
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bioRxiv - Molecular Biology 2020Quote: ... the old medium was replaced with 60 µl HEPES-buffered explant medium supplemented with luciferin (1 mM) (GoldBio), 10% FBS and antibiotics ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were washed once with 1× PBS and 75 μL of 2 mM D-luciferin (LUCK-1G, Goldbio) in Leibovitz’s L-15 medium (21083-027 ...
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bioRxiv - Cancer Biology 2022Quote: ... Isoflurane-anesthetized mice were injected intraperitoneally (i.p.) with 150 mg kg-1 D-luciferin (#LUCNA-1G, Goldbio, USA) and imaged after 5 min using a Newton 7.0 BLI system (Vilber ...
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bioRxiv - Plant Biology 2023Quote: ... seedlings were transferred to media that were pre-sprayed with 0.5 mL of 1 mM D-Luciferin (GOLDBIO) with 0.01% Tween-20 as a surfactant ...
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bioRxiv - Biochemistry 2020Quote: ... washed twice with PBS and once with 50 mM HEPES pH 7.6, and resuspended in lysis buffer (50 mM HEPES pH 7.6, 1% Nonidet-P40, 0.2 mM TCEP (Gold Biotechnology), and Roche cOmplete EDTA-free Protease Inhibitor Cocktail) ...
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bioRxiv - Biophysics 2020Quote: ... 37 °C) to an OD600 of 0.6-0.8 and induced with 0.75 mM isopropyl β-D-1-thiogalactopyranoside (Gold Biotechnology) for 12 h ...
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bioRxiv - Plant Biology 2021Quote: ... The transformants were screened on 0.5× MS plates supplemented with Hygromycin (35 mg/mL, Gold Biotechnology, H-270-1). Candidates were screened for insertion/deletions (indels ...
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bioRxiv - Plant Biology 2021Quote: Fourteen days old plants grown on half strength MS were sprayed with 1 mM Luciferin (Gold Biotechnology, LUCK-1G) with 0.001% triton X-100 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were collected by centrifugation at 6,000g for 20 min and resuspended in a final volume of 40mL cell lysis buffer (100 mM Tris-HCl, pH 8.0, 1 M NaCl, 20% glycerol, 5 mM TCEP (GoldBio), and 2 cOmplete™ EDTA-free protease inhibitor cocktail tablets (Sigma)) ...
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bioRxiv - Biochemistry 2022Quote: ... Bacterial cells were grown at 37°C in Luria Broth and induced at A600 nm = 0.6–0.7 by the addition of 0.3 mM isopropyl-1-thio-β-D-galactopyranoside (Gold Biotechnology) and harvested after 18 h at 18°C ...
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bioRxiv - Biophysics 2023Quote: ... and resuspended in buffer containing 50 mM Tris pH 8.0 and 150 mM NaCl in the presence of protease inhibitors (2.5 μg ml-1 leupeptin (L-010-100, GoldBio), 1 μg ml-1 pepstatin A (P-020-100 ...
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bioRxiv - Biochemistry 2023Quote: ... Bacterial cells were grown at 37°C in Luria Broth and induced at A600 nm = 0.6–0.7 by the addition of 0.3 mM isopropyl-1-thio-β-d-galactopyranoside (Gold Biotechnology) and harvested after growth for 18 hr at 18°C ...
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bioRxiv - Plant Biology 2023Quote: ... Whole leaves were removed after 4 d incubation and leaves were sprayed with a 1 mM D-luciferin (GoldBio) and 0.01% Silwett L-77 (Lehle Seeds Inc ...
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bioRxiv - Biochemistry 2023Quote: ... A 1:100 dilution into 3 L of the same media was grown to OD600 ∼0.25 and induced with 0.4 mM Isopropyl β–D-1-thiogalactopyranoside (IPTG; Goldbio). Cells were harvested ...
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bioRxiv - Microbiology 2019Quote: ... Yale University] were maintained in Luria-Bertani (LB) medium supplemented with antibiotics as appropriate for plasmid selection [50 µg mL-1 kanamycin (GoldBio), 100 µg mL-1 ampicillin (GoldBio ...
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bioRxiv - Plant Biology 2019Quote: 0.5g inflorescence tissue per sample was ground with liquid nitrogen and homogenized in lysis buffer (50mM Tris pH 7.5, 150mM NaCl, 5mM MgCl2, 10% glycerol, 1% IGEPAL, 0.5mM DTT, 1mM PMSF, and 1X GoldBio protease inhibitor) for 15 minutes ...
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bioRxiv - Cell Biology 2021Quote: GST pulldowns were performed by loading 1 µg of purified GST or GST-p130 onto 15 µL of glutathione beads (GoldBio) in NETN (20 mM Tris pH8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... containing 5 mL of digestion buffer (1 mL of Dispase [Corning, 354235], 50 μL of DNase [5 mg/mL, GoldBio, D-301] ...
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bioRxiv - Biochemistry 2021Quote: ... cells were harvested and resuspended in assay buffer containing D-luciferin (0.5 mg ml−1, GoldBio, Cat. no. LUCNA-1G) in 1X HBSS ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5% Triton with 500 μM each of potassium ferrocyanide and potassium ferricyanide and infiltrated with wash buffer as above containing 1 mM X-Gluc (GoldBio). Samples were incubated at 37°C in the dark until staining became visible and were cleared with ethanol for imaging ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then incubated in X-gal staining solution (X-gal rinse buffer plus 1 mg/mL X-gal (GoldBio X4281C ...
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bioRxiv - Cell Biology 2023Quote: ... and added to the cell medium to 1 μM final concentration. D-luciferin sodium salt (Cat. #LUCNA) and coelenterazine (Cat. #CZ) were purchased from GoldBio and resuspended to 100 mM in 10 mM HEPES buffer ...
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bioRxiv - Microbiology 2023Quote: ... New England Biolabs] were grown at 37 °C in Luria-Bertani (LB) medium supplemented with antibiotics as appropriate for plasmid selection [50 µg mL-1 kanamycin (GoldBio), 25 µg mL-1 chloramphenicol (GoldBio) ...
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bioRxiv - Genetics 2023Quote: ... and depleted of non-transposed cells by resuspending the pelleted cells in 300 mL of fresh SCD media containing 1 mg/mL of 5-fluoroorotic acid (ZymoResearch) and 0.1 mg/mL of nourseothricin (Gold Biotechnology) and shaking overnight at 30°C ...
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bioRxiv - Cell Biology 2023Quote: ... The transformants were selected for 4-5 days in 1 ml of YPD broth with clonNAT (200 µg/mL, GoldBio), resulting in the intermediate MTS-mCherry-GFP1-10-clonNat library ...
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bioRxiv - Biochemistry 2024Quote: ... Whole leaves were removed after 4 days of incubation and were sprayed with a solution containing 1 mM D-luciferin (GoldBio) and 0.01% Silwett L-77 (Lehle Seeds Inc ...
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bioRxiv - Biochemistry 2020Quote: ... Single colonies were isolated from YPD (1% yeast extract, 2% peptone, 2% glucose) agar plates containing 400 μg/ml hygromycin (Gold Biotechnology), and integration was verified by PCR ...
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bioRxiv - Plant Biology 2020Quote: ... Chemiluminescence was detected using the NightSHADE LB 985 system (Berthold) in the presence of 20 mg mL−1 potassium luciferin (Gold Biotech). The LUC/REN ratio was calculated to infer effectiveness of miR775 targeting.
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bioRxiv - Biophysics 2020Quote: ... 1% of primary culture was inoculated in 750 mL of LB media and induced with 0.3 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Gold Biotechnology) at OD600 ~ 0.6 and further incubated at 16 °C for 14–16 h with constant shaking at 200 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... cell extraction was carried out as above with the addition of a phosphatase inhibitor cocktail (Simple Stop 1 #GB-450) purchased from Gold Biotechnology, USA ...
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bioRxiv - Biochemistry 2021Quote: ... Transformed cells were grown at 37°C until OD 0.3 then grown at 18°C until induction at OD 0.6 with 1 mM IPTG (Gold Biotechnology, Olivette, MO). Cells were grown for 15 hours then centrifuged after which the pellet was resuspended in 20 mM Sodium Phosphate pH 8 ...
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bioRxiv - Biochemistry 2021Quote: ... expression cultures were grown at 37 °C to an optical density (OD600) of 3.0 before induction with 500 μM isopropyl-β-D-1-thiogalactopyranoside (IPTG; Gold Biotechnology) and incubation overnight at 16 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Luciferase expression was checked by using in vitro bioluminescence imaging by adding 100 μL of 15 mg mL-1 D-luciferin solution (#LUCNA-1G, Goldbio, USA) to the cells and imaged after 2 min using a Fusion FX system (Vilber ...
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bioRxiv - Cancer Biology 2023Quote: ... Luc2 and NLuc expression was confirmed by exposing cells seeded in the wells of a flat-bottom white 96-well plate to 1 volume of 15 mg/mL D-luciferin (#LUCNA-1G, Goldbio, USA) or 1 volume of 0.5 mg/mL Nano-Glo In Vivo Substrate ...
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bioRxiv - Molecular Biology 2023Quote: ... each plate was sprayed with 1 milliliter of an aqueous solution of 5 mM firefly luciferin (Gold Biotechnology, St Louis, MO) and 0.01% (vol/vol ...
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bioRxiv - Microbiology 2023Quote: ... after completion of virus infection virus containing media was replaced with media containing 5 µg/ml MTT reagent (Thiazolyl blue tetrazolium bromide, cat no.298-93-1, Gold biotechnology). Incubate at 37°C for 3-4 hr and then remove MTT containing media and add 100 µl of DMSO to each well to dissolve the formazan crystals formed ...
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bioRxiv - Neuroscience 2023Quote: ... Bacteria were grown to an OD600 of 0.6 and induced with 500 µM isopropyl β-D-1-thioga-lactopyranoside (IPTG) (GoldBio, I2481C) for 18 h at 18°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Approximate tumor size was monitored via bioluminescence imaging (Xenogen IVIS Spectrum Imaging System) 1-2x per week following intraperitoneal injection of luciferin reagent (GOLDBIO, #LUCK-1G) at 150mg/kg ...
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bioRxiv - Biochemistry 2022Quote: ... cells were detached from the plates, resuspended in assay buffer (1XHBSS, 20 mM HEPES, pH7.4) containing D-luciferin (0.5 mg ml-1, GoldBio, Cat. no. LUCNA-1G) and seeded into 96 well white plates (Corning ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli S17-1 λ pir was routinely grown in Luria Bertani (LB) broth (LabExpress) supplemented with 100 μg/mL carbenicillin (Gold Biotechnology) for plasmid selection.
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bioRxiv - Biophysics 2023Quote: ... resuspended in the assay buffer (20mM HEPES pH 7.4 and 1X HBSS) containing 0.5mg ml-1 of D-luciferin (GoldBio, Cat. no: LUCNA-1G) and seeded in 96-well white plates (SPL Life Sciences ...
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bioRxiv - Systems Biology 2023Quote: ... The NAT-resistance cassettes were transformed into FY5 (MATα) and selected on YPD + NAT (200 µg/mL; Gold Biotechnology #N-500-1) plates ...