Labshake search
Citations for Gold Biotechnology :
251 - 300 of 332 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... Bacterial cells were grown at 37°C in Luria Broth and induced at A600 nm = 0.6–0.7 by the addition of 0.3 mM isopropyl-1-thio-β-D-galactopyranoside (Gold Biotechnology) and harvested after 18 h at 18°C ...
-
bioRxiv - Immunology 2024Quote: ... Samples were treated with 1µl D-luciferin (30µg/mL) per 100µl sample volume (Gold Biotechnology, St. Louis, MO, USA) prior to imaging.
-
bioRxiv - Microbiology 2024Quote: Mice received an IP injection of 100 μl of a 2% D-luciferin solution (Goldbio cat #115144-35-9), were anaesthetized with isoflurane gas ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and 120 μl of 20 mg/ml 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-gal, GoldBio) were overlaid on LB agar plates ...
-
bioRxiv - Biochemistry 2023Quote: ... at 37°C in a shaking incubator to the log growth phase (OD600 of 0.6 to 0.8) before being induced with 1 mM isopropyl β-d-1-thiogalactopyranoside (IPTG) (GoldBio). Cultures were then moved to low temperature orbital shakers and incubated at 17°C for 16 hours ...
-
bioRxiv - Biochemistry 2023Quote: ... Bacterial cells were grown at 37°C in Luria Broth and induced at A600 nm = 0.6–0.7 by the addition of 0.3 mM isopropyl-1-thio-β-d-galactopyranoside (Gold Biotechnology) and harvested after growth for 18 hr at 18°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... Mice were intraperitonially injected with 200 μL D-Luciferin (15 mg/mL; Gold Biotechnology, Inc., St. Luis, MO, USA), and Luminescence was taken under anesthesia using IVIS ...
-
bioRxiv - Cancer Biology 2023Quote: ... and imaged within 2-4 minutes after retro-orbital injection of 1.5 mg D-luciferin (GoldBio, Cat: LUCK-10G) dissolved in 100 µL PBS ...
-
bioRxiv - Biochemistry 2023Quote: ... The pellet was resuspended in a solubilization buffer containing 2% n-Dodecyl-B-D-Maltoside (DDM, GoldBio, Cat. # DDM10), 350 mM NaCl in PBS ...
-
bioRxiv - Neuroscience 2023Quote: ... media was exchanged in each well for 30 μL of Assay Buffer containing 1.2 mM of D-Luciferin (GoldBio). Cells were incubated for at least one hour at 37°C before the addition of 15 μl of compounds diluted in series in Assay Buffer ...
-
bioRxiv - Immunology 2023Quote: ... Tumor progression was evaluated by luminescence emission on Xenogen IVIS Spectrum after intraperitoneal injection of 1.5 mg of d-luciferin (GoldBio). Before treatment ...
-
bioRxiv - Biochemistry 2023Quote: ... A 1:100 dilution into 3 L of the same media was grown to OD600 ∼0.25 and induced with 0.4 mM Isopropyl β–D-1-thiogalactopyranoside (IPTG; Goldbio). Cells were harvested ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Plants were screened initially for luciferase expression by spraying 500 µM d-luciferin solution in water (Gold Biotechnology, Inc.) containing 0.01% Tween 20 ...
-
bioRxiv - Neuroscience 2024Quote: ... Mice were intraperitoneally (IP) injected with 200 μL of 15 mg/μL D-Luciferin in PBS (Goldbio, #LUCK-1G), and anaesthetized with 2% isoflurane during imaging ...
-
bioRxiv - Plant Biology 2024Quote: ... Expression of GST-tagged TF proteins was induced between OD600=0.500-0.600 by the addition of 1mM isopropyl-beta-D-thiogalactoside (IPTG) (Goldbio: I2481C25) and 0.05M or 0.1M D-mannitol to bacteria in Lysogeny Broth ...
-
bioRxiv - Synthetic Biology 2024Quote: ... the culture was induced with a final concentration of 1 mM isopropyl B-D-1-thiogalatopyranoside (IPTG) (GoldBio, I2481C). Once DO reached 50% ...
-
bioRxiv - Synthetic Biology 2024Quote: ... the culture was induced with a final concentration of 1 mM isopropyl B-D-1-thiogalatopyranoside (IPTG) (GoldBio, I2481C). Once DO reached 50% ...
-
bioRxiv - Biochemistry 2024Quote: ... Expression of the indicated proteins was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG) (I2481C; GoldBio, MO, USA) (0.5 mM final concentration ...
-
bioRxiv - Biochemistry 2024Quote: ... Extract was cleared by centrifugation at 12,000 rpm for 30 min prior to 2h incubation with nickel affinity beads for Ubp2 purification (GOLDBIO catalog H-355) or glutathione-containing beads (GOLDBIO G-250-5 ...
-
bioRxiv - Biochemistry 2024Quote: ... Media supernatant was further filtered using 0.2 μm PVDF vacuum filter prior to incubation with Ni-NTA agarose resin (GoldBio, H-350-5) (10 mL wet volume per 1 L supernatant ...
-
bioRxiv - Microbiology 2020Quote: ... The mice were anesthetized in isoflurane and imaged after intraperitoneal injection of 150 mg/kg of D-luciferin (Gold Biotechnology). Bioluminescence imaging of MHV68-M3-FL infected mice was collected on the IVIS Lumina Series III (Perkin Elmer) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Mice received an intraperitoneal injection of 200 mg/kg D-luciferin firefly potassium salt diluted in PBS 15 minutes before imaging (GoldBio).
-
bioRxiv - Microbiology 2020Quote: ... Mice infected with Fluc-carrying viruses received in vivo grade D-Luciferin (15 mg/mL in sterile endotoxin-free PBS, GoldBio) (r.o. ...
-
bioRxiv - Biochemistry 2021Quote: ... cells were harvested and resuspended in assay buffer containing D-luciferin (0.5 mg ml−1, GoldBio, Cat. no. LUCNA-1G) in 1X HBSS ...
-
bioRxiv - Microbiology 2021Quote: ... Mice were each injected (i.p.) with 100 µl of 25 mg/ml D-luciferin potassium salt (GoldBio, St. Louis, MO) and imaged daily from days 3 dpi - 7 dpi with an In Vivo Imaging System (IVIS ...
-
bioRxiv - Immunology 2020Quote: ... Cells were plated at equal density and luminescence signals were quantified in the presence of 150 µg/ml D-luciferin (GoldBio) by imaging using cooled CCD optical macroscopic imaging system (IVIS Lumina Series III ...
-
bioRxiv - Developmental Biology 2022Quote: ... 50 μL of DNase (stock: 5 mg/mL; final concentration: 0.025 mg/mL or 50 U/ml, GoldBio, D-301), and 100 μL of Collagenase Type I (stock ...
-
bioRxiv - Biochemistry 2024Quote: ... Whole leaves were removed after 4 days of incubation and were sprayed with a solution containing 1 mM D-luciferin (GoldBio) and 0.01% Silwett L-77 (Lehle Seeds Inc ...
-
bioRxiv - Biochemistry 2024Quote: ... Two-hundred μl of cells were mixed with 200 μl of 5 mM D-luciferin K+ salt (GoldBio, LUCK-100) diluted in Tyrode’s buffer and incubated at 28 °C for 15 minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... the mice were injected intraperitoneally with 10 μl/g of 15 mg/mL D-luciferin potassium salt solution (Gold Biotechnology) and imaged using the Xenogen IVIS 200 In Vivo Imaging System (PerkinElmer) ...
-
bioRxiv - Cell Biology 2023Quote: ... and added to the cell medium to 1 μM final concentration. D-luciferin sodium salt (Cat. #LUCNA) and coelenterazine (Cat. #CZ) were purchased from GoldBio and resuspended to 100 mM in 10 mM HEPES buffer ...
-
bioRxiv - Cancer Biology 2023Quote: ... 15 minutes after subcutaneous injection of 100 μl (150 mg Luciferin/kg body weight) of D-luciferin (Goldbio LUCK-1G). After the final week ...
-
bioRxiv - Cell Biology 2023Quote: ... Bacteria grew at 37°C and protein induction occurred at 20°C for overnight upon addition of 0.1mM isopropyl D-1-thigalactopyranoside (IPTG; Goldbio, I2481C) to the bacterial culture at mid-log phase (OD600nm=0.6) ...
-
bioRxiv - Biophysics 2024Quote: ... Cultures were incubated at 37 °C until the absorbance at 600 nm reached 0.7-0.9 at which point 1 mM isopropyl β-d-1-thiogalactopyranoside (IPTG) (Gold Biotechnology) was added ...
-
bioRxiv - Cancer Biology 2024Quote: ... Orthotopic and tail-vein implanted mouse lung metastases were assessed by serial IVIS imaging after administration of 2mg/mouse D-Luciferin (GOLDBIO). Bioluminescence images were captured using the IVIS spectrum CT In Vivo imaging system (Caliper Life Sciences) ...
-
bioRxiv - Cancer Biology 2024Quote: ... mice were anesthetized and injected retro-orbitally with 1.5 mg (75 mg/kg) of D-luciferin (Gold Biotechnology; LUCK-1G) at the indicated times after injecting cells ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were treated with increasing BIA doses for 5 hours and then exposed to 10 µg/ml of D-Luciferin (Goldbio). Luminescence signal was quantified in the IVIS system.
-
bioRxiv - Cancer Biology 2022Quote: ... mice were anesthetized with 2.5% isoflurane (Fujifilm Wako) and intraperitoneally injected with 3 mg D-luciferin (Gold Biotechnology Inc., MO, USA) in 200 μl PBS as previously described [52 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Bioluminescence signals from luciferase-expressing cells in live mice were recorded 10 minutes after intraperitoneal injection of D-luciferin (80 mg/kg) (Gold Biotechnology) with IVIS Lumina III Imaging System (PerkinElmer) ...
-
bioRxiv - Biophysics 2020Quote: ... 1% of primary culture was inoculated in 750 mL of LB media and induced with 0.3 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Gold Biotechnology) at OD600 ~ 0.6 and further incubated at 16 °C for 14–16 h with constant shaking at 200 rpm ...
-
bioRxiv - Biochemistry 2021Quote: ... expression cultures were grown at 37 °C to an optical density (OD600) of 3.0 before induction with 500 μM isopropyl-β-D-1-thiogalactopyranoside (IPTG; Gold Biotechnology) and incubation overnight at 16 °C ...
-
bioRxiv - Physiology 2021Quote: ... in a sealed petri dish containing air-buffered bioluminescence medium (Yamazaki and Takahashi, 2005) plus D-luciferin (100 μM) (Gold Biotechnology) and incubated at 32 °C as previously described (van der Vinne et al. ...
-
bioRxiv - Physiology 2021Quote: ... sections containing SCN (150 μm) were cultured on a Millicell membrane in air-buffered media containing 100 μM D-luciferin (Gold Biotechnology) and imaged for 5 days using a Stanford Photonics XR/MEGA-10Z cooled intensified charge-coupled device camera.
-
bioRxiv - Cancer Biology 2022Quote: ... recipe of FACS buffer was RPMI-1640 + 5% FBS + 2% BSA + 50 µg/ml DNase-I (Gold Biotechnology, D-301-500). For all other primary cell staining ...
-
bioRxiv - Cancer Biology 2022Quote: ... Luciferase expression was checked by using in vitro bioluminescence imaging by adding 100 μL of 15 mg mL-1 D-luciferin solution (#LUCNA-1G, Goldbio, USA) to the cells and imaged after 2 min using a Fusion FX system (Vilber ...
-
bioRxiv - Neuroscience 2023Quote: ... Bacteria were grown to an OD600 of 0.6 and induced with 500 µM isopropyl β-D-1-thioga-lactopyranoside (IPTG) (GoldBio, I2481C) for 18 h at 18°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... Tumor volume was monitored through bioluminescence using IVIS Spectrum apparatus (Caliper Life Sciences) after i.p injection of D-luciferin 150mg/Kg (GOLDBIO #LUCK-1G). Mice were sacrificed when manifested more than 20% of body weight loss or neurological symptoms ...
-
bioRxiv - Biochemistry 2024Quote: ... media in the wells was replaced with the assay buffer (20mM HEPES pH 7.4, 1X Hank’s Balanced Salt Solution/ HBSS and 0.5mg/mL D-luciferin (GoldBio, Cat. no: LUCNA-1G). Luminescence was recorded immediately in a microplate reader (BMG Labtech) ...
-
bioRxiv - Biochemistry 2024Quote: ... resuspended in assay buffer (20mM HEPES pH 7.4, 1X Hank’s Balanced Salt Solution/ HBSS and 0.5mg/mL D-luciferin (GoldBio, Cat. no: LUCNA-1G) and seeded in 96-well plates at a density of 100,000 cells/well ...
-
bioRxiv - Neuroscience 2023Quote: ... Injected postnatal day one old mice were returned to the dam and after one week injected intraperitoneally with 150mg/kg firefly D-luciferin (sterile filtered 15mg/ml in phosphate buffered saline, Gold Biotechnology) (48 ...