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Citations for Gold Biotechnology :
201 - 247 of 247 citations for PGFM ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... 37 °C) to an OD600 of 0.6-0.8 and induced with 0.75 mM isopropyl β-D-1-thiogalactopyranoside (Gold Biotechnology) for 12 h ...
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bioRxiv - Plant Biology 2021Quote: Fourteen days old plants grown on half strength MS were sprayed with 1 mM Luciferin (Gold Biotechnology, LUCK-1G) with 0.001% triton X-100 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were collected by centrifugation at 6,000g for 20 min and resuspended in a final volume of 40mL cell lysis buffer (100 mM Tris-HCl, pH 8.0, 1 M NaCl, 20% glycerol, 5 mM TCEP (GoldBio), and 2 cOmplete™ EDTA-free protease inhibitor cocktail tablets (Sigma)) ...
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bioRxiv - Biochemistry 2022Quote: ... Bacterial cells were grown at 37°C in Luria Broth and induced at A600 nm = 0.6–0.7 by the addition of 0.3 mM isopropyl-1-thio-β-D-galactopyranoside (Gold Biotechnology) and harvested after 18 h at 18°C ...
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bioRxiv - Biophysics 2023Quote: ... and resuspended in buffer containing 50 mM Tris pH 8.0 and 150 mM NaCl in the presence of protease inhibitors (2.5 μg ml-1 leupeptin (L-010-100, GoldBio), 1 μg ml-1 pepstatin A (P-020-100 ...
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bioRxiv - Biochemistry 2023Quote: ... Bacterial cells were grown at 37°C in Luria Broth and induced at A600 nm = 0.6–0.7 by the addition of 0.3 mM isopropyl-1-thio-β-d-galactopyranoside (Gold Biotechnology) and harvested after growth for 18 hr at 18°C ...
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bioRxiv - Plant Biology 2023Quote: ... Whole leaves were removed after 4 d incubation and leaves were sprayed with a 1 mM D-luciferin (GoldBio) and 0.01% Silwett L-77 (Lehle Seeds Inc ...
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bioRxiv - Biochemistry 2023Quote: ... A 1:100 dilution into 3 L of the same media was grown to OD600 ∼0.25 and induced with 0.4 mM Isopropyl β–D-1-thiogalactopyranoside (IPTG; Goldbio). Cells were harvested ...
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bioRxiv - Cancer Biology 2024Quote: ... Blots were blocked for 1 h at RT in 1X TBST + 3% BSA Fraction V (Gold Biotechnology, Olivette, MO) and then incubated in primary antibody diluted in blocking buffer with overnight rocking at 4°C (phosphorylated and total EGFR ...
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bioRxiv - Microbiology 2021Quote: ... Lysate was centrifuged at 18,000xg for 1 hr and the supernatant containing soluble protein was bound to nickel agarose beads (Gold Biotechnology), washed with buffer containing 25mM Tris HCl (pH 7.5) ...
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bioRxiv - Microbiology 2019Quote: ... Yale University] were maintained in Luria-Bertani (LB) medium supplemented with antibiotics as appropriate for plasmid selection [50 µg mL-1 kanamycin (GoldBio), 100 µg mL-1 ampicillin (GoldBio ...
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bioRxiv - Plant Biology 2019Quote: 0.5g inflorescence tissue per sample was ground with liquid nitrogen and homogenized in lysis buffer (50mM Tris pH 7.5, 150mM NaCl, 5mM MgCl2, 10% glycerol, 1% IGEPAL, 0.5mM DTT, 1mM PMSF, and 1X GoldBio protease inhibitor) for 15 minutes ...
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bioRxiv - Cell Biology 2021Quote: GST pulldowns were performed by loading 1 µg of purified GST or GST-p130 onto 15 µL of glutathione beads (GoldBio) in NETN (20 mM Tris pH8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... containing 5 mL of digestion buffer (1 mL of Dispase [Corning, 354235], 50 μL of DNase [5 mg/mL, GoldBio, D-301] ...
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bioRxiv - Biochemistry 2021Quote: ... cells were harvested and resuspended in assay buffer containing D-luciferin (0.5 mg ml−1, GoldBio, Cat. no. LUCNA-1G) in 1X HBSS ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5% Triton with 500 μM each of potassium ferrocyanide and potassium ferricyanide and infiltrated with wash buffer as above containing 1 mM X-Gluc (GoldBio). Samples were incubated at 37°C in the dark until staining became visible and were cleared with ethanol for imaging ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then incubated in X-gal staining solution (X-gal rinse buffer plus 1 mg/mL X-gal (GoldBio X4281C ...
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bioRxiv - Cell Biology 2023Quote: ... and added to the cell medium to 1 μM final concentration. D-luciferin sodium salt (Cat. #LUCNA) and coelenterazine (Cat. #CZ) were purchased from GoldBio and resuspended to 100 mM in 10 mM HEPES buffer ...
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bioRxiv - Microbiology 2023Quote: ... New England Biolabs] were grown at 37 °C in Luria-Bertani (LB) medium supplemented with antibiotics as appropriate for plasmid selection [50 µg mL-1 kanamycin (GoldBio), 25 µg mL-1 chloramphenicol (GoldBio) ...
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bioRxiv - Genetics 2023Quote: ... and depleted of non-transposed cells by resuspending the pelleted cells in 300 mL of fresh SCD media containing 1 mg/mL of 5-fluoroorotic acid (ZymoResearch) and 0.1 mg/mL of nourseothricin (Gold Biotechnology) and shaking overnight at 30°C ...
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bioRxiv - Cell Biology 2023Quote: ... Bacteria grew at 37°C and protein induction occurred at 20°C for overnight upon addition of 0.1mM isopropyl D-1-thigalactopyranoside (IPTG; Goldbio, I2481C) to the bacterial culture at mid-log phase (OD600nm=0.6) ...
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bioRxiv - Cell Biology 2023Quote: ... The transformants were selected for 4-5 days in 1 ml of YPD broth with clonNAT (200 µg/mL, GoldBio), resulting in the intermediate MTS-mCherry-GFP1-10-clonNat library ...
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bioRxiv - Biochemistry 2024Quote: ... Whole leaves were removed after 4 days of incubation and were sprayed with a solution containing 1 mM D-luciferin (GoldBio) and 0.01% Silwett L-77 (Lehle Seeds Inc ...
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bioRxiv - Biophysics 2019Quote: ... at which point protein expression was induced by the addition of 0.5 mM isopropyl 1-thio-β-D-galactopyranoside (Gold Biotechnology). Temperature was reduced to 16°C for overnight expression ...
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bioRxiv - Plant Biology 2020Quote: ... Chemiluminescence was detected using the NightSHADE LB 985 system (Berthold) in the presence of 20 mg mL−1 potassium luciferin (Gold Biotech). The LUC/REN ratio was calculated to infer effectiveness of miR775 targeting.
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bioRxiv - Biophysics 2020Quote: ... 1% of primary culture was inoculated in 750 mL of LB media and induced with 0.3 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Gold Biotechnology) at OD600 ~ 0.6 and further incubated at 16 °C for 14–16 h with constant shaking at 200 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... cell extraction was carried out as above with the addition of a phosphatase inhibitor cocktail (Simple Stop 1 #GB-450) purchased from Gold Biotechnology, USA ...
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bioRxiv - Biochemistry 2021Quote: ... Transformed cells were grown at 37°C until OD 0.3 then grown at 18°C until induction at OD 0.6 with 1 mM IPTG (Gold Biotechnology, Olivette, MO). Cells were grown for 15 hours then centrifuged after which the pellet was resuspended in 20 mM Sodium Phosphate pH 8 ...
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bioRxiv - Biochemistry 2021Quote: ... expression cultures were grown at 37 °C to an optical density (OD600) of 3.0 before induction with 500 μM isopropyl-β-D-1-thiogalactopyranoside (IPTG; Gold Biotechnology) and incubation overnight at 16 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Luciferase expression was checked by using in vitro bioluminescence imaging by adding 100 μL of 15 mg mL-1 D-luciferin solution (#LUCNA-1G, Goldbio, USA) to the cells and imaged after 2 min using a Fusion FX system (Vilber ...
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bioRxiv - Microbiology 2023Quote: ... after completion of virus infection virus containing media was replaced with media containing 5 µg/ml MTT reagent (Thiazolyl blue tetrazolium bromide, cat no.298-93-1, Gold biotechnology). Incubate at 37°C for 3-4 hr and then remove MTT containing media and add 100 µl of DMSO to each well to dissolve the formazan crystals formed ...
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bioRxiv - Neuroscience 2023Quote: ... Bacteria were grown to an OD600 of 0.6 and induced with 500 µM isopropyl β-D-1-thioga-lactopyranoside (IPTG) (GoldBio, I2481C) for 18 h at 18°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Approximate tumor size was monitored via bioluminescence imaging (Xenogen IVIS Spectrum Imaging System) 1-2x per week following intraperitoneal injection of luciferin reagent (GOLDBIO, #LUCK-1G) at 150mg/kg ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli S17-1 λ pir was routinely grown in Luria Bertani (LB) broth (LabExpress) supplemented with 100 μg/mL carbenicillin (Gold Biotechnology) for plasmid selection.
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bioRxiv - Biophysics 2023Quote: ... resuspended in the assay buffer (20mM HEPES pH 7.4 and 1X HBSS) containing 0.5mg ml-1 of D-luciferin (GoldBio, Cat. no: LUCNA-1G) and seeded in 96-well white plates (SPL Life Sciences ...
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bioRxiv - Systems Biology 2023Quote: ... The NAT-resistance cassettes were transformed into FY5 (MATα) and selected on YPD + NAT (200 µg/mL; Gold Biotechnology #N-500-1) plates ...
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bioRxiv - Plant Biology 2020Quote: ... 200 mM IAA in ETOH was first diluted in sterile water and then added to hydroponically growing rice roots approximately 1 day after germination to create a final concentration of 200 nM (GoldBio, I-110-25) ...
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bioRxiv - Genomics 2021Quote: ... and then stained with the indicated antibody in FACS buffer (PBS with 1% FBS, 10 U/ml DNAse I, GoldBio, St. Louis MO) for 30 minutes on ice with gentle agitation ...
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bioRxiv - Biochemistry 2022Quote: ... in the medium with the exception of the 293T-based CAPN5-3×FLAG stable cell line that was selected and maintained with 200 µg/ml hygromycin (Gold Biotechnology, H-270-1).
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bioRxiv - Microbiology 2021Quote: ... Fecal samples were collected regularly over 2-6 weeks and plated onto SAB and SAB with nourseothricin (100 μg/mL, Gold Biotechnology, N-500-1) plates.
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bioRxiv - Biochemistry 2022Quote: ... the cleared cell lysate was loaded onto a gravity column with 1 mL bed volume of Glutathione agarose resin (Gold Biotechnology, #G-250-5). The resin was extensively washed ...
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bioRxiv - Cell Biology 2022Quote: KO2 cells of an early passage were infected with lentiviruses expressing HMGB2- eYFP or FOXM1-eYFP fusion proteins and selected with 20 µg/ml hygromycin B (Goldbio Biotechnology, H-270-1). Pools of cells were then cultured in the presence of hygromycin ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were probed with the primary antibodies listed in Supplemental Table 1 overnight in 5% bovine serum albumin (Gold Biotechnology, Cat.# A-420-100) in TBST ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was filtered and then incubated with nutation at 4°C for 1 hr with Ni-NTA agarose (Gold Biotechnology #H-350-100) that was pre-equilibrated with lysis buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... CAS NO 3483-12-3), isopropyl-β-thiogalactopyranoside (IPTG, CAS NO 367-93-1) and bovine serum albumin (BSA) were obtained from Gold Biotechnology (Burlington, MA). Alexa Fluor 594 C5 maleimide was purchased from BroadPharm (San Diego ...
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bioRxiv - Plant Biology 2020Quote: Histochemical GUS staining was done by submerging leaf cuttings in the GUS staining solution containing 1 mM X-Gluc (Gold Biotechnologies, St. Louis, MO, USA) according to Jefferson et al ...
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bioRxiv - Cell Biology 2021Quote: ... tricornutum cell line (maintained in the Allen Lab; McQuaid et al., 2018) was additionally supplemented with 200 μg/mL nourseothricin (GoldBio, Saint Louis, MO, Catalog #N-500-1). All transconjugant P ...