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Citations for Gold Biotechnology :
151 - 200 of 224 citations for Mac 1 SAP rat Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... according to the selectable marker expressed by the lentiviral vector (GoldBio, Cat#: H-270-1). Dox-inducible myrAKT-HA fibroblasts (FIG 4 ...
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bioRxiv - Plant Biology 2024Quote: ... seedlings were rinsed with 1/2MS with 10 µM Timentin (Gold Biotechnology, Cat# T104-2), transferred into a fresh 1/2MS medium ...
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bioRxiv - Biophysics 2019Quote: ... at which point SP expression was induced with 1 mM IPTG (Gold Biotechnology, St Louis, MO). The cells were grown for a further 16 h at 18 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Expression was induced with 0.1 mM isopropyl-1-thio-β-D-galactopyranoside (IPTG; Goldbio, Cat#I2481C25) for 18 hours ...
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bioRxiv - Biochemistry 2020Quote: ... Overexpression was carried out upon induction with 0.1 mM isopropyl β-D-1-thiogalactopyranoside (Gold Biotechnology) for 18-20 hours at 18 °C ...
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bioRxiv - Biophysics 2022Quote: ... at which time HnSpns expression was induced by the addition of 1 mM IPTG (Gold Biotechnology). The cultures were incubated overnight (∼15 h ...
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bioRxiv - Biochemistry 2022Quote: Isopropyl β-D-1-thiogalactopyranoside (IPTG) and 5-aminolevulinic acid (5-ALA) were purchased from GoldBio. NNG was purchased from AAblocks ...
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bioRxiv - Genetics 2023Quote: ... followed by the addition of 500 mM IPTG (β-D-1-thiogalactopyranoside, Gold Biotechnology, Catalog #I2481C25) for protein expression and incubation at 18°C for 15-18 hrs ...
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DeCOIL: Optimization of Degenerate Codon Libraries for Machine Learning-Assisted Protein EngineeringbioRxiv - Bioengineering 2023Quote: ... Protein expression is induced with the addition of 50 μL of IPTG (GoldBio 367-93-1) to a 1 mM final concentration ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked in 5% bovine serum albumin (BSA, #A-420-1, Gold Biotechnology, St. Louis, MO), for phospho-specific antibodies ...
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bioRxiv - Genetics 2022Quote: ... 500 μl of 4 mg/ml stock of Hygromycin solution (Gold Biotechnology, catalog no. H-270-1) was topically added to the bacterial lawn of injection plates to select for transgenic (F1 ...
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bioRxiv - Plant Biology 2023Quote: ... Whole leaves were removed after 4 d incubation and sprayed with a 1 mM D-Luciferin (GoldBio) and 0.01% Silwet L-77 (Lehle Seeds Inc ...
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bioRxiv - Plant Biology 2023Quote: ... The primary transformants were screened on ½ MS plates containing 15 µg ml-1 hygromycin B (Gold Biotechnology). After 2 weeks ...
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bioRxiv - Cell Biology 2024Quote: ... Molecular mass standards for gels were 100 bp and 1 kb DNA ladders (GoldBio, St. Louis, MO).
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bioRxiv - Cell Biology 2020Quote: ... bacterial cells were grown at 37°C in Lucia Broth medium and induced at A600 nm = 0.6-0.7 by the addition of 0.1 mM isopropyl-1-thio-□-D-galactopyranoside (Gold Biotechnology) and harvested after 18h at 18°C ...
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bioRxiv - Biophysics 2019Quote: ... 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (HEPES) and Dithiothreitol (DTT) were purchased from GoldBio (St Louis, MO, US). Ethylene Glycol-bis(β-aminoethyl ether)-N,N,N′,N′-Tetraacetic Acid (EGTA) ...
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bioRxiv - Molecular Biology 2020Quote: ... the old medium was replaced with 60 µl HEPES-buffered explant medium supplemented with luciferin (1 mM) (GoldBio), 10% FBS and antibiotics ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were washed once with 1× PBS and 75 μL of 2 mM D-luciferin (LUCK-1G, Goldbio) in Leibovitz’s L-15 medium (21083-027 ...
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bioRxiv - Cancer Biology 2022Quote: ... Isoflurane-anesthetized mice were injected intraperitoneally (i.p.) with 150 mg kg-1 D-luciferin (#LUCNA-1G, Goldbio, USA) and imaged after 5 min using a Newton 7.0 BLI system (Vilber ...
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bioRxiv - Biochemistry 2022Quote: ... protein expression was induced by adding 600 μL of 1 M isopropyl β-D-thiogalactopyranoside (IPTG, Gold Biotechnology) to a final concentration of 1 mM ...
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bioRxiv - Plant Biology 2023Quote: ... seedlings were transferred to media that were pre-sprayed with 0.5 mL of 1 mM D-Luciferin (GOLDBIO) with 0.01% Tween-20 as a surfactant ...
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bioRxiv - Biochemistry 2020Quote: ... washed twice with PBS and once with 50 mM HEPES pH 7.6, and resuspended in lysis buffer (50 mM HEPES pH 7.6, 1% Nonidet-P40, 0.2 mM TCEP (Gold Biotechnology), and Roche cOmplete EDTA-free Protease Inhibitor Cocktail) ...
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bioRxiv - Biophysics 2020Quote: ... 37 °C) to an OD600 of 0.6-0.8 and induced with 0.75 mM isopropyl β-D-1-thiogalactopyranoside (Gold Biotechnology) for 12 h ...
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bioRxiv - Plant Biology 2021Quote: ... The transformants were screened on 0.5× MS plates supplemented with Hygromycin (35 mg/mL, Gold Biotechnology, H-270-1). Candidates were screened for insertion/deletions (indels ...
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bioRxiv - Plant Biology 2021Quote: Fourteen days old plants grown on half strength MS were sprayed with 1 mM Luciferin (Gold Biotechnology, LUCK-1G) with 0.001% triton X-100 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were collected by centrifugation at 6,000g for 20 min and resuspended in a final volume of 40mL cell lysis buffer (100 mM Tris-HCl, pH 8.0, 1 M NaCl, 20% glycerol, 5 mM TCEP (GoldBio), and 2 cOmplete™ EDTA-free protease inhibitor cocktail tablets (Sigma)) ...
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bioRxiv - Biochemistry 2022Quote: ... Bacterial cells were grown at 37°C in Luria Broth and induced at A600 nm = 0.6–0.7 by the addition of 0.3 mM isopropyl-1-thio-β-D-galactopyranoside (Gold Biotechnology) and harvested after 18 h at 18°C ...
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bioRxiv - Biophysics 2023Quote: ... and resuspended in buffer containing 50 mM Tris pH 8.0 and 150 mM NaCl in the presence of protease inhibitors (2.5 μg ml-1 leupeptin (L-010-100, GoldBio), 1 μg ml-1 pepstatin A (P-020-100 ...
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bioRxiv - Biochemistry 2023Quote: ... Bacterial cells were grown at 37°C in Luria Broth and induced at A600 nm = 0.6–0.7 by the addition of 0.3 mM isopropyl-1-thio-β-d-galactopyranoside (Gold Biotechnology) and harvested after growth for 18 hr at 18°C ...
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bioRxiv - Plant Biology 2023Quote: ... Whole leaves were removed after 4 d incubation and leaves were sprayed with a 1 mM D-luciferin (GoldBio) and 0.01% Silwett L-77 (Lehle Seeds Inc ...
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bioRxiv - Biochemistry 2023Quote: ... A 1:100 dilution into 3 L of the same media was grown to OD600 ∼0.25 and induced with 0.4 mM Isopropyl β–D-1-thiogalactopyranoside (IPTG; Goldbio). Cells were harvested ...
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bioRxiv - Cancer Biology 2024Quote: ... Blots were blocked for 1 h at RT in 1X TBST + 3% BSA Fraction V (Gold Biotechnology, Olivette, MO) and then incubated in primary antibody diluted in blocking buffer with overnight rocking at 4°C (phosphorylated and total EGFR ...
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bioRxiv - Microbiology 2021Quote: ... Lysate was centrifuged at 18,000xg for 1 hr and the supernatant containing soluble protein was bound to nickel agarose beads (Gold Biotechnology), washed with buffer containing 25mM Tris HCl (pH 7.5) ...
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bioRxiv - Microbiology 2019Quote: ... Yale University] were maintained in Luria-Bertani (LB) medium supplemented with antibiotics as appropriate for plasmid selection [50 µg mL-1 kanamycin (GoldBio), 100 µg mL-1 ampicillin (GoldBio ...
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bioRxiv - Plant Biology 2019Quote: 0.5g inflorescence tissue per sample was ground with liquid nitrogen and homogenized in lysis buffer (50mM Tris pH 7.5, 150mM NaCl, 5mM MgCl2, 10% glycerol, 1% IGEPAL, 0.5mM DTT, 1mM PMSF, and 1X GoldBio protease inhibitor) for 15 minutes ...
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bioRxiv - Cell Biology 2021Quote: GST pulldowns were performed by loading 1 µg of purified GST or GST-p130 onto 15 µL of glutathione beads (GoldBio) in NETN (20 mM Tris pH8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... containing 5 mL of digestion buffer (1 mL of Dispase [Corning, 354235], 50 μL of DNase [5 mg/mL, GoldBio, D-301] ...
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bioRxiv - Biochemistry 2021Quote: ... cells were harvested and resuspended in assay buffer containing D-luciferin (0.5 mg ml−1, GoldBio, Cat. no. LUCNA-1G) in 1X HBSS ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5% Triton with 500 μM each of potassium ferrocyanide and potassium ferricyanide and infiltrated with wash buffer as above containing 1 mM X-Gluc (GoldBio). Samples were incubated at 37°C in the dark until staining became visible and were cleared with ethanol for imaging ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then incubated in X-gal staining solution (X-gal rinse buffer plus 1 mg/mL X-gal (GoldBio X4281C ...
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bioRxiv - Cell Biology 2023Quote: ... and added to the cell medium to 1 μM final concentration. D-luciferin sodium salt (Cat. #LUCNA) and coelenterazine (Cat. #CZ) were purchased from GoldBio and resuspended to 100 mM in 10 mM HEPES buffer ...
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bioRxiv - Microbiology 2023Quote: ... New England Biolabs] were grown at 37 °C in Luria-Bertani (LB) medium supplemented with antibiotics as appropriate for plasmid selection [50 µg mL-1 kanamycin (GoldBio), 25 µg mL-1 chloramphenicol (GoldBio) ...
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bioRxiv - Genetics 2023Quote: ... and depleted of non-transposed cells by resuspending the pelleted cells in 300 mL of fresh SCD media containing 1 mg/mL of 5-fluoroorotic acid (ZymoResearch) and 0.1 mg/mL of nourseothricin (Gold Biotechnology) and shaking overnight at 30°C ...
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bioRxiv - Cell Biology 2023Quote: ... Bacteria grew at 37°C and protein induction occurred at 20°C for overnight upon addition of 0.1mM isopropyl D-1-thigalactopyranoside (IPTG; Goldbio, I2481C) to the bacterial culture at mid-log phase (OD600nm=0.6) ...
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bioRxiv - Cell Biology 2023Quote: ... The transformants were selected for 4-5 days in 1 ml of YPD broth with clonNAT (200 µg/mL, GoldBio), resulting in the intermediate MTS-mCherry-GFP1-10-clonNat library ...
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bioRxiv - Biochemistry 2024Quote: ... Whole leaves were removed after 4 days of incubation and were sprayed with a solution containing 1 mM D-luciferin (GoldBio) and 0.01% Silwett L-77 (Lehle Seeds Inc ...
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bioRxiv - Biophysics 2019Quote: ... at which point protein expression was induced by the addition of 0.5 mM isopropyl 1-thio-β-D-galactopyranoside (Gold Biotechnology). Temperature was reduced to 16°C for overnight expression ...
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bioRxiv - Biochemistry 2020Quote: ... Single colonies were isolated from YPD (1% yeast extract, 2% peptone, 2% glucose) agar plates containing 400 μg/ml hygromycin (Gold Biotechnology), and integration was verified by PCR ...
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bioRxiv - Plant Biology 2020Quote: ... Chemiluminescence was detected using the NightSHADE LB 985 system (Berthold) in the presence of 20 mg mL−1 potassium luciferin (Gold Biotech). The LUC/REN ratio was calculated to infer effectiveness of miR775 targeting.
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bioRxiv - Biophysics 2020Quote: ... 1% of primary culture was inoculated in 750 mL of LB media and induced with 0.3 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Gold Biotechnology) at OD600 ~ 0.6 and further incubated at 16 °C for 14–16 h with constant shaking at 200 rpm ...