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Citations for Gold Biotechnology :
101 - 138 of 138 citations for DNA Damage 8 OHdG ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Single colonies from the selection plates were grown in selective LB media containing 50 μg/mL ampicillin (Goldbio Cat #: A-301-55) overnight in a 37 °C shaking incubator (180 rpm) ...
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bioRxiv - Cell Biology 2024Quote: ... Molecular mass standards for gels were 100 bp and 1 kb DNA ladders (GoldBio, St. Louis, MO).
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bioRxiv - Biophysics 2021Quote: ... and resuspended in assay buffer (HBSS with 20 mM HEPES pH 7.45) with 5 μg/mL freshly prepared coelenterazine 400a (GoldBio). Cells were then placed in white-walled ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were collected by centrifugation at 6,000g for 20 min and resuspended in a final volume of 40mL cell lysis buffer (100 mM Tris-HCl, pH 8.0, 1 M NaCl, 20% glycerol, 5 mM TCEP (GoldBio), and 2 cOmplete™ EDTA-free protease inhibitor cocktail tablets (Sigma)) ...
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bioRxiv - Molecular Biology 2022Quote: ... and 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal) for blue white selection (Gold Biotechnology, 2016).
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bioRxiv - Biochemistry 2022Quote: ... then the cleared bacterial lysate was passed through a 0.22 μm PES filter (Genessee Scientific) and then applied to a 5 mL packed Ni-NTA agarose beads (Goldbio) equilibrated with Lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... with all purification buffers for full-length WT NEMO also containing 5 mM Tris(2-carboxyethyl)phosphine (TCEP) (GoldBio) to minimize the formation of disulfide0bonded aggregates ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 120 μl of 20 mg/ml 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-gal, GoldBio) were overlaid on LB agar plates ...
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bioRxiv - Biochemistry 2023Quote: ... ten-fold serial dilutions were plated onto synthetic complete agar lacking histidine and containing 0.1% w/v 5-fluoroorotic acid (GoldBio). Plasmid shuffling experiments involving Use1 were conducted in an analogous manner ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were transferred onto fresh media and treated with 100 µg/ml Carbenicillin (GoldBio, C-103-5, lot # 0129.091814A).
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bioRxiv - Biophysics 2024Quote: ... Cleared lysate was loaded onto lysis buffer-equilibrated Ni-NTA resin (GoldBio, 5 mL resin per 50 mL lysate) and rocked gently for 1 h at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... or fog-2(q71) along with approximately 30 wild type males were placed on RNAi plates [MYOB containing 1mM IPTG (GoldBio, Cat. No. I2481C) and seeded with the appropriate dsRNA expressing HT115 cells] ...
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bioRxiv - Molecular Biology 2024Quote: ... the following were added directly to worm plates 3 days after injection: HygR selection −100 ul of 20 mg/ml hygromycin B (GoldBio, St. Louis, MO), NeoR selection - 500 ul of 25 mg/ml G418 disulfate (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: Purification of the mouse lectin-mIgG2a fusion proteins was performed using Protein A agarose resin (Gold Biotechnology, P-400-5). The protein A beads were pelleted at 150g for 5 min and washed once with 1x binding buffer (0.02 M Sodium Phosphate ...
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bioRxiv - Microbiology 2020Quote: ... The protein was eluted with 5 mL of elution buffer (20 mM TEA, 50 mM KCl, 500 mM imidazole (GoldBio), pH 7) ...
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bioRxiv - Microbiology 2020Quote: ... The protein eluted and collected in 5-1mL aliquots of elution buffer (50 mM NaH2PO4, 300 mM NaCl, and 250 mM imidazole (GoldBio) (pH 8.0)) ...
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bioRxiv - Developmental Biology 2022Quote: ... 50 μL of DNase (stock: 5 mg/mL; final concentration: 0.025 mg/mL or 50 U/ml, GoldBio, D-301), and 100 μL of Collagenase Type I (stock ...
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bioRxiv - Genetics 2023Quote: ... and depleted of non-transposed cells by resuspending the pelleted cells in 300 mL of fresh SCD media containing 1 mg/mL of 5-fluoroorotic acid (ZymoResearch) and 0.1 mg/mL of nourseothricin (Gold Biotechnology) and shaking overnight at 30°C ...
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bioRxiv - Cell Biology 2023Quote: ... The transformants were selected for 4-5 days in 1 ml of YPD broth with clonNAT (200 µg/mL, GoldBio), resulting in the intermediate MTS-mCherry-GFP1-10-clonNat library ...
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bioRxiv - Biochemistry 2024Quote: ... Two-hundred μl of cells were mixed with 200 μl of 5 mM D-luciferin K+ salt (GoldBio, LUCK-100) diluted in Tyrode’s buffer and incubated at 28 °C for 15 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Correct genotypes were selected on YES plates with the corresponding antibiotic (200 μg/mL G418, Sigma; 100 μg/mL Nourseothricin, GoldBio; 100 μg/mL phleomycin, Invivogen). Other strains were created by mating and antibiotic selection ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were resuspended in 3 ml assay buffer (HBSS buffer with 0.01% BSA and 5 mM HEPES, pH 7.4) containing 10 μM coelenterazine (Goldbio, Cat. no: CZ05) at final concentration ...
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bioRxiv - Cancer Biology 2022Quote: ... recipe of FACS buffer was RPMI-1640 + 5% FBS + 2% BSA + 50 µg/ml DNase-I (Gold Biotechnology, D-301-500). For all other primary cell staining ...
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bioRxiv - Biochemistry 2022Quote: ... and resuspended in assay buffer (1XHBSS, 5 mM HEPES, pH 7.4, 0.01% BSA) containing 10 μM coelenterazine (GoldBio, Cat no. CZ2.5). Resuspended cells were seeded in a white flat bottom 96-well plate (100 μl well-1) ...
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bioRxiv - Biochemistry 2023Quote: ... and resuspended in the NanoBiT assay buffer (HBSS containing 0.01% BSA, 5 mM HEPES, pH 7.4, and 10 μM coelenterazine (GoldBio, Cat no: CZ05). After resuspension ...
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bioRxiv - Microbiology 2023Quote: ... after completion of virus infection virus containing media was replaced with media containing 5 µg/ml MTT reagent (Thiazolyl blue tetrazolium bromide, cat no.298-93-1, Gold biotechnology). Incubate at 37°C for 3-4 hr and then remove MTT containing media and add 100 µl of DMSO to each well to dissolve the formazan crystals formed ...
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bioRxiv - Microbiology 2020Quote: ... Strains were patched onto medium containing 50 g/ml X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside; Gold Biotechnology), 1 mM IPTG ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed with lysis buffer (50 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM n-Dodecyl-B-D-maltoside (DDM) (GoldBio, catalog #: DDM-5)) supplemented with protease inhibitor cocktail (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... were transformed and plated on LB Agar (Fisher BioReagents Cat#: BP1425-500) containing 50 µg/mL carbenicillin (GoldBio Cat#: C-103-5) and incubated overnight at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... The G418-resistance cassettes were transformed into FY4 (MATa) and selected on YPD + G418 (400 µg/mL; Gold Biotechnology #G-418-5) plates ...
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bioRxiv - Genetics 2021Quote: ... After extraction for 30 min at RT samples were centrifuged 600g 3 min and supernatants were precleared with 15μl of protein A agarose beads (Goldbio, cat. P-400-5) for 2 hours at RT ...
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bioRxiv - Biochemistry 2022Quote: ... the cleared cell lysate was loaded onto a gravity column with 1 mL bed volume of Glutathione agarose resin (Gold Biotechnology, #G-250-5). The resin was extensively washed ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were probed with the primary antibodies listed in Supplemental Table 1 overnight in 5% bovine serum albumin (Gold Biotechnology, Cat.# A-420-100) in TBST ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 5-bromo-4-chloro-3-indolyl-β-D-galactoside (X- GAL) (X4281C10) for blue-white screening were purchased from Gold Biotechnology (Olivette, Missouri). Lysozyme (L6876-10G) ...
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bioRxiv - Genomics 2022Quote: ... then the mixed libraries were separated on 1.5% agarose gel for 45 min at 95V along with 50 bp (Fisher, Hampton, NH, USA) and 100 bp DNA ladders (GoldBio, St. Louis, MO, USA). The sharp band at ~286 bp was excised and purified with gel extraction kit (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... containing ProBlock Gold Extra Strength Protease Inhibitor Cocktail Kit (GoldBio, Cat. #: GB-116-2). The cortical lysate was then aliquoted into a 96 well plate and stored at −80°C.
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bioRxiv - Synthetic Biology 2019Quote: ... using the Illumination™ Firefly & Renilla Luciferase Enhanced Assay Kit (Goldbio®, cat# I-920) per manufacturer instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were lysed and assessed for NF-κB activity with an Illumination Firefly Luciferase Enhanced Assay Kit (Gold Biotechnology). The results were normalized by samples’ protein concentrations.