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Citations for Gold Biotechnology :
101 - 150 of 1191 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant contains solubilized mitochondrial proteins and mixed with 5% G-250 (GoldBio, cat. C-460-5) and 1x NativePAGE sample buffer (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Coelenterazine (GoldBio, Cat #CZ) was resuspended to 10 mM in ethanol and stored protected from light ...
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bioRxiv - Cell Biology 2024Quote: ... D-luciferin (Goldbio, Cat# LUCNA-100) was resuspended in 10 mM HEPES buffer and used at 150 μg/mL final concentration ...
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bioRxiv - Microbiology 2024Quote: ... A Nickel-Nitrilotriacetic acid (Ni-NTA, GOLDBIO) column was washed with water and equilibrated in Buffer A containing 20 mM imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... Luminescence was measured for 2 s in a Lumat LB 9507 (Berthold Technologies) after injection of 100 µl 250 μM Luciferin (Gold Biotechnology). Luciferase Refolding served as a measure for disaggregation activity ...
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bioRxiv - Developmental Biology 2024Quote: ... the buffer was replaced with a fresh incubation buffer (which is same as above, but contains 50 µM puromycin (GoldBio P-600-100)) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 150 mg/kg D-Luciferin (Goldbio) was administered via IP injection ...
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bioRxiv - Biochemistry 2024Quote: ... The soluble fraction was then applied to a 5 mL packed Ni-NTA resin bed (GoldBio) and equilibrated with 10 column volumes of TBS ...
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bioRxiv - Biochemistry 2024Quote: ... Extract was cleared by centrifugation at 12,000 rpm for 30 min prior to 2h incubation with nickel affinity beads for Ubp2 purification (GOLDBIO catalog H-355) or glutathione-containing beads (GOLDBIO G-250-5 ...
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bioRxiv - Pathology 2024Quote: SAECs were lysed in RIPA buffer containing phosphatases and proteases inhibitors (Gold Biotechnology), centrifuged at 13000xg for 10minutes ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with DTT (GoldBio), and proteins were detected with a mouse antibody to GFP (1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... Mice received luciferin in their water (10 mM, Gold Biotechnology) ad libitum over the 2 days of recording ...
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bioRxiv - Neuroscience 2024Quote: ... slides were incubated in nitro blue tetrazolium (NBT, 100mg/mL in 70% dimethyl formamide/30% DEPC-H20, Gold Biotechnology, St. Louis, MO) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP ...
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bioRxiv - Neuroscience 2024Quote: ... and 5-bromo-4-chloro-3-indolyl phosphate (BCIP, 50mg/mL in 100% dimethyl formamide, Gold Biotechnology, St. Louis, MO) in NTMT ...
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bioRxiv - Molecular Biology 2024Quote: ... mice were injected with 150 mg/kg D-luciferin (Gold Biotechnology) intraperitoneally and bioluminescence was acquired using IVIS Lumina (Revvity ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 % Pen/Strep and 5 µg/ml Puromycin (Goldbio). Cells were seeded into collagen-coated 24-well plates (50 µg/ml ...
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bioRxiv - Bioengineering 2024Quote: ... ∼2 mL of 2% low melting-point agarose (GoldBio, Cat# A-204-100) in ASW was pipetted into the imaging spacer until it completely fills the chamber ...
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bioRxiv - Biophysics 2024Quote: ... and 0.25 mg/mL bovine serum albumin (BSA) (GoldBio #A-420-250)) ...
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bioRxiv - Biophysics 2024Quote: ... Isopropyl-β-D-thiogalactoside (IPTG, 1 mM; Gold Biotechnology, cat I2481C50) was used for induction and cells were grown for another 15 hours at 25° C ...
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bioRxiv - Biophysics 2024Quote: ... with added antibiotics (100 µg/ml of ampicillin, Sisco Research Laboratory, cat 61314 or 50 µg/ml kanamycin monosulfate, Gold Biotechnology, cat K-120-5) till OD600 reached 0.6 ...
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bioRxiv - Cancer Biology 2024Quote: ... mice were injected with 150 mg/kg of D-luciferin (LUCK-4G, Gold Biotechnologies), and imaged using the IVIS Spectrum Imaging System 15 minutes post-injection.
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bioRxiv - Cancer Biology 2024Quote: Tumor growth was monitored by bioluminescence on a Xenogen IVIS Spectrum 10 min after intraperitoneal injection of 150 mg/kg D-luciferin prepared according to the manufacturer’s directions (Gold Biotechnology, #LUCK-100). The total flux signal was used for quantification in all studies.
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bioRxiv - Neuroscience 2024Quote: ... Coelenterazine 400a (5uM, GoldBIO.com) diluted in BRET buffer (In mM ...
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bioRxiv - Neuroscience 2024Quote: ... sodium salt (GoldBio, Cat. eLUCNA) and Gibco™ DPBS (no calcium ...
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bioRxiv - Biophysics 2024Quote: ... Cultures were incubated at 37 °C until the absorbance at 600 nm reached 0.7-0.9 at which point 1 mM isopropyl β-d-1-thiogalactopyranoside (IPTG) (Gold Biotechnology) was added ...
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bioRxiv - Biophysics 2024Quote: ... supplemented with 1 mM dithiothreitol (DTT) (Gold Biotechnology), and subsequently centrifuged at 200,000xg for 1.5 hours to isolate the membranes.
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bioRxiv - Biophysics 2024Quote: ... Transformant colonies were used to inoculate Luria-Bertani (LB) media (LabExpress) containing 0.1 mg/mL ampicillin (Gold Biotechnology). Overnight colonies were grown at 34 °C for approximately 16 hours and used to inoculate minimal medium A at a 3:200 dilution ...
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bioRxiv - Biophysics 2024Quote: ... Cells were harvested by centrifugation at 5,000xg and were resuspended in 40 mL of lysis buffer (100 mM KPi, pH 7.5) supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) (Gold Biotechnology). Cells were lysed by sonication on ice with continuous stirring ...
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bioRxiv - Biophysics 2024Quote: ... puromycin dihydrochloride (Goldbio, Catalog Number P-600-100) was added to a final concentration of 2 mM and the centrifuge tubes were inverted to mix ...
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bioRxiv - Cancer Biology 2024Quote: ... Orthotopic and tail-vein implanted mouse lung metastases were assessed by serial IVIS imaging after administration of 2mg/mouse D-Luciferin (GOLDBIO). Bioluminescence images were captured using the IVIS spectrum CT In Vivo imaging system (Caliper Life Sciences) ...
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bioRxiv - Cancer Biology 2024Quote: ... CLEC18A-Fc fusion was purified from the supernatant with an Protein A agarose resin (Gold Biotechnology). Protein A beads in an ethanol solution were centrifuged at 150 x g for 5 min to remove the ethanol solution ...
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bioRxiv - Cell Biology 2024Quote: ... and RNaseA from Gold Biotechnology. ANTI-FLAG® M2 Affinity Gel was purchased from Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: Samples were triple-rinsed with phosphate buffer before using X-gal (Goldbio) for rhizobia staining ...
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bioRxiv - Biochemistry 2024Quote: ... all STU and MTU constructs were transformed into Agrobacterium tumefaciens GV3101 cells (Goldbio) by electroporation as previously described (Kamileen et al. ...
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bioRxiv - Biochemistry 2024Quote: ... 2.5× MS vitamin mixture and 500 μg/mL of Timentin (GoldBio, USA)) until harvested for analysis.
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bioRxiv - Bioengineering 2024Quote: ... The culture was then quickly cooled on ice for 10-15 minutes and induced with 1 mM isopropyl ß-D-1-thiogalactopyranoside (IPTG) (Gold Biotechnology, Cat# I2481C100). For CODE constructs that were built based on the pET-PE2-His backbone (Addgene ...
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bioRxiv - Biochemistry 2024Quote: ... the culture was cooled to 22°C and grown overnight after addition of 1 ml of 1 M IPTG (GoldBio). Cells were harvested by centrifugation at 6000 rpm for 30 min at 4°C and resuspended in binding buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... TCEP hydrochloride was purchased from Gold Biotechnology. Cyanine5 maleimide was purchased from Lumiprobe ...
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bioRxiv - Biophysics 2024Quote: ... 4 °C) and the resulting supernatant was incubated with His-tag cobalt resin (GoldBio) for 1 hr ...
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bioRxiv - Biophysics 2024Quote: All solvents and reagents were purchased from commercial sources (Sigma-Aldrich, VWR, GoldBio, EMD, P3 Bio Systems, etc.) and used without further purification ...
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bioRxiv - Bioengineering 2024Quote: ... D-Luciferin (Gold Biotechnology, catalog no. LUCNA-2G) at 150 μg/ml was added to the cells and incubated for 5 minutes at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... B27 containing “Air DMEM media” containing 0.1mM D-luciferin (Goldbio), sealed with vacuum grease ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µL of media from each well was added to 10 µL of non-lytic 2x coelenterazine reagent (300 mM sodium ascorbate, 5 mM NaCl, 0.1% BSA, 40 μM coelenterazine (Goldbio CZ25)) in separate wells of a 384-well plate ...
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bioRxiv - Microbiology 2024Quote: ... and 1% egg white lysozyme (GoldBio) solution (20 mg/ml in 50 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... 200 μg/ml gentamicin (Goldbio) and 25 μg/ml erythromycin (VWR).
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bioRxiv - Microbiology 2024Quote: ... and 50 mg of egg white lysozyme (Gold Biotechnology)) ...
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bioRxiv - Microbiology 2024Quote: ... and 1% egg white lysozyme (GoldBio) solution (20 mg/ml in 50 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... The membrane is then saturated in 5% BSA (GoldBio #A-420) in TBS-Tween and then probed overnight at 4C with primaries antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM dithiothreitol (cat # DTT10, GoldBio; St Louis, MO), 500 µM ascorbic acid ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.28 mg/ml luciferin (Goldbio LUCK-1G)) ...