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Citations for Gold Biotechnology :
51 - 100 of 157 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted by adding lysis buffer containing 35 mM reduced glutathione (Gold Biotechnology). The elute fractions were run on a 4-12% NuPAGE Bis-Tris protein gel (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... 500 μl of 4 mg/ml stock of Hygromycin solution (Gold Biotechnology, catalog no. H-270-1) was topically added to the bacterial lawn of injection plates to select for transgenic (F1 ...
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bioRxiv - Plant Biology 2023Quote: ... Whole leaves were removed after 4 d incubation and sprayed with a 1 mM D-Luciferin (GoldBio) and 0.01% Silwet L-77 (Lehle Seeds Inc ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were clarified via centrifugation and applied to 4 mL of pre-equilibrated Ni+-NTA resin (GoldBio) in a gravity flow column ...
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bioRxiv - Biophysics 2019Quote: ... protein expression was then induced with Isopropyl-β-D-1-thiogalactopyranoside 0.1 mM (GoldBio, Canada) for 12 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were clarified using centrifugation and proteins were purified via nickel agarose beads (Gold Biotechnology), eluted with 300mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... along with 2.5 μl of a pre-stained protein ladder (#P008; GoldBio, St. Louis, MO), and separated on an SDS-polyacrylamide gel ...
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bioRxiv - Immunology 2022Quote: ... 4.5 mg E1D1 antibody was incubated with 1 mL Protein A resin (GoldBio, P-400) with rotation at room temperature for 30 minutes and then washed thoroughly with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Lipin 1 and lipin 1 ΔM-Lip proteins were purified using Ni-NTA resin (GoldBio). Eluted protein was applied to Streptactin-XT resin equilibrated with equilibration buffer (100 mM Tris ...
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bioRxiv - Immunology 2020Quote: Supernatant containing RBD-Fc was purified over protein A agarose resin (Goldbio, Cat# P-400), cleaved with HRV3C protease (made in house ...
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bioRxiv - Immunology 2021Quote: ... All GST fusion proteins were purified using Glutathione Agarose 4B (Gold Biotechnology, #G-250-50), and eluted with HEPES buffered saline (HBS ...
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bioRxiv - Biophysics 2019Quote: ... 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (HEPES) and Dithiothreitol (DTT) were purchased from GoldBio (St Louis, MO, US). Ethylene Glycol-bis(β-aminoethyl ether)-N,N,N′,N′-Tetraacetic Acid (EGTA) ...
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bioRxiv - Biochemistry 2020Quote: ... The hexahistidine-tagged proteins were affinity purified from soluble lysate using nickel agarose beads (Gold Biotechnology). Bound protein was washed with 50 mL lysis buffer before elution using 5 mL of elution buffer containing 25 mM Tris HCl pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... the GltPh proteins were reduced with 500 µM tris(2-carboxy-ethyl) phosphine (TCEP, Gold Biotechnology) for 30 min at room temperature ...
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DeCOIL: Optimization of Degenerate Codon Libraries for Machine Learning-Assisted Protein EngineeringbioRxiv - Bioengineering 2023Quote: ... Protein expression is induced with the addition of 50 μL of IPTG (GoldBio 367-93-1) to a 1 mM final concentration ...
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bioRxiv - Biochemistry 2020Quote: ... and His SUMO tags were cleaved with Ulp1 protease overnight at 4°C in Buffer C (25mM Tris-HCl pH 8.5, 150mM KCl, 2mM TCEP (GoldBio), 50mM Imidazole ...
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bioRxiv - Molecular Biology 2022Quote: ... and 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal) for blue white selection (Gold Biotechnology, 2016).
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bioRxiv - Synthetic Biology 2023Quote: ... and 120 μl of 20 mg/ml 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-gal, GoldBio) were overlaid on LB agar plates ...
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bioRxiv - Cancer Biology 2023Quote: ... and imaged within 2-4 minutes after retro-orbital injection of 1.5 mg D-luciferin (GoldBio, Cat: LUCK-10G) dissolved in 100 µL PBS ...
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bioRxiv - Plant Biology 2023Quote: ... Whole leaves were removed after 4 d incubation and leaves were sprayed with a 1 mM D-luciferin (GoldBio) and 0.01% Silwett L-77 (Lehle Seeds Inc ...
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bioRxiv - Microbiology 2020Quote: ... The hexahistidine-tagged DXR protein was affinity purified from soluble lysate via nickel agarose beads (Gold Biotechnology). Bound protein was eluted in 300 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... The His6-tagged protein variants present in the supernatant were purified using Ni2+-NTA affinity chromatography (Goldbio) and dialyzed overnight at 4 ⁰C against 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2019Quote: ... Lysates were clarified using centrifugation and proteins were purified via nickel agarose beads (Gold Biotechnology, Olivette, MO), eluted with 300mM imidazole ...
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bioRxiv - Microbiology 2019Quote: ... The hexahistidine-tagged DXR protein was affinity purified from soluble lysate via nickel agarose beads (Gold Biotechnology). Bound protein was eluted in 300 mM imidazole ...
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bioRxiv - Molecular Biology 2022Quote: ... 120 µg protein was mixed with substrate containing 15mM ONPG (2-Nitrophenyl-β-D-galactopyranoside, Goldbio, #N27510), 5mM DTT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Equal volumes of each sample were loaded onto a 4-12% SDS-PAGE gel along with the BLUEstainTM 2 (GoldBio) protein ladder ...
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bioRxiv - Biochemistry 2022Quote: ... #DF0446-07-5) or 7H10 (Difco, cat# DF0627-17-4) plates containing 50 ug/mL hygromycin (GoldBio, cat. #H-270) and incubated at 37° C for 3-5 days ...
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bioRxiv - Cell Biology 2023Quote: ... The transformants were selected for 4-5 days in 1 ml of YPD broth with clonNAT (200 µg/mL, GoldBio), resulting in the intermediate MTS-mCherry-GFP1-10-clonNat library ...
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bioRxiv - Biochemistry 2024Quote: ... Whole leaves were removed after 4 days of incubation and were sprayed with a solution containing 1 mM D-luciferin (GoldBio) and 0.01% Silwett L-77 (Lehle Seeds Inc ...
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bioRxiv - Cancer Biology 2023Quote: ... 4×10e7 cfu were plated on-trp Gal/Raf supplemented with 1mg/ml 5-fluoroorotic acid (5-FOA) (Gold Biotechnology) and were grown at 30 degrees for 96 hours ...
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bioRxiv - Biophysics 2020Quote: ... and protein expression was induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (Goldbio, St Lousi, MO, USA). After induction ...
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bioRxiv - Bioengineering 2022Quote: ... Protein expression was induced by the addition of 1mM isopro-pyl-L-thio-D-galactopyranoside (IPTG) (Gold Biotechnology) at 37°c and after 35 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... and protein expression was induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (Goldbio, St Lousi, MO, USA). After induction ...
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bioRxiv - Biochemistry 2022Quote: ... protein expression was induced by adding 600 μL of 1 M isopropyl β-D-thiogalactopyranoside (IPTG, Gold Biotechnology) to a final concentration of 1 mM ...
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bioRxiv - Biophysics 2023Quote: ... The temperature was then set to 30 °C and protein expression was induced by adding 0.2 % arabinose (Goldbio). Cells were grown for additional 16 hours ...
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bioRxiv - Biochemistry 2023Quote: ... Culture growth at 37 °C was monitored by measuring absorbance at 600 nm (A600) and protein expression was induced at A600 = ∼0.7 with 0.5 mM IPTG (GoldBio). The culture was grown at 18 °C for ∼15 hr ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein expression was induced for ∼6 h by 1 mM isopropyl β-a-D-1-thiogalactopyranoside (IPTG; GoldBio) in an 18 °C shaker ...
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bioRxiv - Molecular Biology 2021Quote: ... Cultures were cold-shocked on ice for 1 hour and protein expression was induced with 1 mM IPTG (GoldBio). Culture induction was continued for 16 hours 18 °C while shaking ...
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bioRxiv - Microbiology 2020Quote: ... Strains were patched onto medium containing 50 g/ml X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside; Gold Biotechnology), 1 mM IPTG ...
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bioRxiv - Biochemistry 2023Quote: ... 20 mM of 4-(2- hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) pH 7.4 and D-luciferin (0.5 mg ml-1) (GoldBio, Cat. no.: LUCNA-1G). Harvested cells were then seeded in an opaque flat bottom white 96 well cell culture plate (SPL life sciences ...
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bioRxiv - Microbiology 2021Quote: ... Lysate was centrifuged at 18,000xg for 1 hr and the supernatant containing soluble protein was bound to nickel agarose beads (Gold Biotechnology), washed with buffer containing 25mM Tris HCl (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... The protein was eluted with 5 mL of elution buffer (20 mM TEA, 50 mM KCl, 500 mM imidazole (GoldBio), pH 7) ...
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bioRxiv - Cell Biology 2023Quote: ... Bacteria grew at 37°C and protein induction occurred at 20°C for overnight upon addition of 0.1mM isopropyl D-1-thigalactopyranoside (IPTG; Goldbio, I2481C) to the bacterial culture at mid-log phase (OD600nm=0.6) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were harvested by centrifugation (6000xg for 20 min) and lysed with B-PER Bacterial Protein Extraction Reagent (Thermo Fischer) supplemented with DNAse I (GoldBio). Lysate was incubated at 60°C for 20 min and cleared by centrifugation at 14,000xg for 30 min ...
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bioRxiv - Bioengineering 2024Quote: ... Mice were imaged on the IVIS every 3-4 days after being sedated with Isoflurane and intraperitoneal injections of ∼120 mg/kg D-luciferin (GoldBio, St. Louis, MI). Mice were injected with 100,000 IU of human IL-2 (National Cancer Institute ...
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bioRxiv - Biophysics 2019Quote: ... at which point protein expression was induced by the addition of 0.5 mM isopropyl 1-thio-β-D-galactopyranoside (Gold Biotechnology). Temperature was reduced to 16°C for overnight expression ...
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bioRxiv - Cell Biology 2021Quote: ... 200 µl diluted cells (2 x104 cells) and 50 µl (8 mg/ml) D-luciferin (GoldBio, USA) were loaded into each well ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was filtered and then incubated with nutation at 4°C for 1 hr with Ni-NTA agarose (Gold Biotechnology #H-350-100) that was pre-equilibrated with lysis buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 5-bromo-4-chloro-3-indolyl-β-D-galactoside (X- GAL) (X4281C10) for blue-white screening were purchased from Gold Biotechnology (Olivette, Missouri). Lysozyme (L6876-10G) ...
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bioRxiv - Biophysics 2023Quote: ... the temperature was reduced to 20 °C and protein expression was induced with the addition of IPTG (GoldBio, St. Louis, MO, USA) to a final concentration of 1 mM ...