Labshake search
Citations for BMG Labtech :
1 - 50 of 113 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Cells were grown to ~90% confluency in a 96-well plate prior to addition of serially diluted virus to achieve luminescence signals of 30,000-1,000,000 (BMG Labtech PolarStar Optima plate reader at maximum gain) ...
-
Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The solution was centrifuged 16,000g for 10 minutes at 4°C and the supernatant containing the mitochondrial or cell lysate proteins quantified (Bio-Rad DC Protein Assay, BMG Labtech POLARstar Omega plate reader).
-
bioRxiv - Microbiology 2020Quote: ... Virus was subsequently added (up to 10% viral supernatant by volume) to achieve uninhibited luminescence signals of >30,000 (BMG Labtech PolarStar Optima plate reader at maximum gain ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cell viability was measured using CellTiter Glo 2 reagent and CLARIOstar Plus microplate reader (BMG Labtech).
-
bioRxiv - Physiology 2021Quote: ... cells were washed 2 × with D-PBS and immediately transferred to a plate reader (ClarioStar, BMG Labtech), where fluorescent CellROX oxidation products were excited at 640 nm and light emission detected at 665 nm ...
-
bioRxiv - Immunology 2020Quote: ... and the cells were lysed with 2% SDS before the absorbance at 550 nm was measured on FLUOstar OMEGA microplate reader (BMG Labtech, UK). Non-coated wells were also seeded and adhesion results were compared between the two cell genotypes ...
-
bioRxiv - Genomics 2021Quote: ... Protein concentration was measured using the Pierce™ BCA Protein Assay Kit on the POLARstar® Omega microplate reader (BMG Labtech). 7μg of protein lysate was diluted with 4x Laemmli Sample Buffer (BioRad cat # 1610747 ...
-
Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... Proteins were suspended in 50mM ammonium bicarbonate in 8M urea and then quantified (Bio-Rad DC Protein Assay, BMG Labtech POLARstar Omega plate reader). To reduce disulphide bonds ...
-
bioRxiv - Plant Biology 2022Quote: ... selected for Paromomycin resistance were grown in 200μl TAP in 96-well plates under 50μmol photons m-2 s-2 for 3-5 days and then screened for Venus expression in a fluorescence plate reader (CLARIOstar, BMG Labtech) as described previously (Caspari ...
-
bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were then measured using a CLARIOstar plate reader (BMG Labtech), and sample concentrations calculated based on the BSA standard curve.
-
bioRxiv - Cancer Biology 2019Quote: ... Colour change was read and protein quantifications calculated using PolarStar Omega (BMG Labtech) and Mars-data analysis software (BMG Labtech).
-
bioRxiv - Microbiology 2023Quote: ... Protein fluorescence was measured at 25 °C on a CLARIOstar multimode plate reader (BMG Labtech), with excitation at 295 ± 10 nm ...
-
bioRxiv - Molecular Biology 2021Quote: ... with readings taken at 2 min intervals using a multiplate reader (CLARIOstar, BMG Labtech). PMNs were added in the dark ...
-
bioRxiv - Biochemistry 2024Quote: ... for 2 min before measuring luminescence in a POLARstar OMEGA plate reader (BMG Labtech) at 28°C ...
-
bioRxiv - Cancer Biology 2021Quote: ... After protein concentration determination using a UV/VIS Spectrometer at 280 nm (SPECTROstar Nano, BMG Labtech), each sample was spiked with fixed ratios of E ...
-
Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The mitochondrial suspension was quantified (Bio-Rad DC Protein Assay, BMG Labtech POLARstar Omega plate reader) and then suspended in miR05 respiration buffer (110mM D-sucrose ...
-
bioRxiv - Biochemistry 2020Quote: ... for 2 min before measuring luminescence signals in a POLARstar OMEGA plate reader (BMG Labtech) at 28 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... The signal between 2 and 9.5 min was used for baseline subtraction (Mars software, BMG Labtech).
-
bioRxiv - Synthetic Biology 2023Quote: ... The fluorescent signals were recorded every 2 mins using a plate reader (ClarioStar Plus, BMG Labtech).
-
bioRxiv - Plant Biology 2024Quote: ... Luminescence was captured over 2 hours (90 cycles) using a PHERAstar FS plate reader (BMG Labtech) controlled through the PHERAstar control software ...
-
bioRxiv - Physiology 2019Quote: ... using 1 to 2 µL aliquots of the sample in a PolarStar Omega (BMG labtech, Ortenberg, Germany) [17].
-
bioRxiv - Bioengineering 2022Quote: ... WST-1 assay was assessed by absorbance after 2 hours with an Omega PolarStar (BMG Labtech, Germany). Live imaging was performed using a Zeiss Observer Z1 inverted microscope (Zeiss ...
-
bioRxiv - Cell Biology 2023Quote: ... Plates were then centrifuged (2k rpm, 2 min) before being analysed in a PheraStar FS (BMG Labtech) under the following conditions ...
-
bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations were determined by UV spectrophotometry at 280 nm using a FLUOstar Omega multi-detection microplate reader (BMG LABTECH). The resulting values were plotted using GraphPad Prism 7.04 and the PEG midpoint score was derived from the midpoint of the sigmoidal dose-response (variable slope ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Absorbance for protein concentration measurements was acquired at 280 nm on a SPECTROstar® Nano Plate Reader (BMG Labtech, Germany) using UV-transparent 96 well plates.
-
bioRxiv - Neuroscience 2022Quote: ... baseline absorbance was read for 2 minutes at 340nm and 25°C using a SPECTROstar Nano (BMG Labtech). After recording of the baseline ...
-
bioRxiv - Neuroscience 2022Quote: ... baseline absorbance was read for 2 minutes at 412nm at 25°C using a SPECTROstar Nano (BMG Labtech). After recording of the baseline ...
-
bioRxiv - Cancer Biology 2020Quote: ... Protein quantification was performed according to the bicinchoninic acid method (Pierce BCA Protein Assay Kit, Life Tech) using the OMEGA plate reader (BMG Labtech) at 562nm ...
-
bioRxiv - Cell Biology 2023Quote: ... 100 nM of FITC-labeled CPI motif peptide and varying concentration of yeast capping protein were incubated for 15 minutes in room temperature before the fluorescence anisotropy was measured with ClarioStar plate reader (BMG labtech). Anisotropy values were calculated with MARS data analysis software (BMG labtech) ...
-
bioRxiv - Immunology 2024Quote: ... the OD600 of liposomes without added protein was measured for two minutes on a Clariostar plus plate reader (BMG Labtech, Germany) before WT or R188A CRP (1000 nM ...
-
bioRxiv - Molecular Biology 2022Quote: ... plates were read out several times over the next 1 to 2 days using a PHERAstar FX (BMG Labtech) plate reader ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein concentration was measured using the Pierce BCA Protein Assay Kit (ThermoScientific) and the absorbance at 562 nm was evaluated using a CLARIOstar® microplate reader (BMG Labtech) or a TriStar plate reader (Berthold) ...
-
bioRxiv - Molecular Biology 2020Quote: ... and the time course of fluorescence-intensity (see Fig. 2) was recorded with a microplate reader (POLARstar OPTIMA, BMG LABTECH) over 16 hours ...
-
bioRxiv - Biochemistry 2021Quote: ... 1 mM DTT and 2 mM ATPγS and measured in 384-well non-stick black plates using ClarioStar PLUS (BMG LabTech) at room temperature ...
-
bioRxiv - Biochemistry 2020Quote: ... Fluorescence anisotropy of the prepared samples was measured at 25°C for 2 h using a plate reader (CLARIOstar, BMG Labtech, Excitation ...
-
bioRxiv - Biochemistry 2022Quote: ... in a ratio of 2:1:1 and monitoring the absorption at 340 nm for 200-300 s in a microplate reader (CLARIOstar, BMG Labtech). Protein activity was calculated according to the Lambert-Beer law and converted to the observed catalytic activity at the given substrate concentration of 1 mM (kobs) ...
-
bioRxiv - Plant Biology 2021Quote: ... 50 µl ½ MS containing DMSO or chemicals were added for a final concentration of 5 µM DPI or 25 µM PI and luminescence was measured at 2 min intervals by orbital scan mode in a LUMIstar Omega plate reader (BMG Labtech). After 1 h ...
-
bioRxiv - Microbiology 2022Quote: ... Turbidity was monitored in 2 min intervals at 30 °C in flat-bottom 96 well plates using a FLUOstar OMEGA plate reader (BMG LABTECH). Plates were agitated before each measurement ...
-
bioRxiv - Cell Biology 2020Quote: ... ligand was added and luminescence measured every 2 min for kinetics data or after 20 min for drug response curves using a Pherastar FS (BMG labtech) plate reader.
-
bioRxiv - Immunology 2019Quote: ... Reactions were stopped with 50 μL/well 2 M H2SO4 and read at 450 and 570 nm on a FLUOstar Omega microplate reader (BMG Labtech). Data were analyzed with Microsoft Excel and GraphPad Prism 6.0 ...
-
bioRxiv - Neuroscience 2022Quote: ... Lysates (or lysis buffer alone as a background control) were incubated for 2 h at 37 °C before reading on a fluorescence plate reader (BMG LABTECH) at excitation 360 nm ...
-
bioRxiv - Cancer Biology 2022Quote: ... A 2-hour incubation was used and fluorescence (excitation 560, emission 590O was measured via FLUOstar Omega plate reader (BMG Labtech). Anoikis resistance for each condition was calculated by creating a ratio of the viability of the low-adhesion plate challenged cells to the positive control normal-adhesion plated cells for cells from each treatment condition ...
-
bioRxiv - Cell Biology 2022Quote: ... CCK-8 solution for 2 h and the absorption was recorded at 450nm by using a microplate reader (POLARstar Omega, BMG LABTECH) according to the manufacturer’s protocol.
-
bioRxiv - Immunology 2023Quote: ... fluorescence (485nm excitation, 535nm emission) was measured every 2 minutes for 60 minutes total on the CLARIOstar plate reader (BMG Labtech). Baseline measurements were determined by incubation of liposomes with LUV buffer alone ...
-
bioRxiv - Microbiology 2024Quote: ... The translation of the reporter mRNA was monitored every 2 min by measuring YFP fluorescence was measured on a CLARIOstar plate reader (BMG Labtech).
-
bioRxiv - Bioengineering 2024Quote: ... 5% CO2 for 2 h after which MTS absorbance was measured at 490 nm using a plate reader (BMG Labtech Clariostar). For the LDH assay ...
-
bioRxiv - Cell Biology 2024Quote: ... and Calcium Green-5N fluorescence (excitation 506 nm, emission 531 nm) was monitored every 2 s at RT using a CLARIOstar microplate reader (BMG Labtech) after injection of 40 µM CaCl2 ...
-
bioRxiv - Biochemistry 2024Quote: ... followed by incubation in the dark at room temperature for 2 min before measuring luminescence in a POLARstar OMEGA plate reader (BMG Labtech). Measurements and data processing were done as described in “BRET measurements in HEK293T cells” except that measurements were taken every 0.96 s (with a signal integration time of 0.96 s) ...
-
bioRxiv - Biochemistry 2024Quote: ... followed by incubation in the dark for 2 min at room temperature before measuring luminescence in a POLARstar OMEGA plate reader (BMG Labtech). Measurements and data processing were done as described in “BRET measurements in HEK293T cells” except that measurements with Gαi3/Gβγ ONEGO were made every 0.48 s (with a signal integration time of 0.48 s).
-
bioRxiv - Cancer Biology 2020Quote: ... The plate was incubated at 37°C for 2 hours before the absorbance is measured at 490nm using a POLARstar OPTIMA plate reader (BMG Labtech, UK). Readings were blank corrected by subtracting cell free controls and data normalised to untreated control.