Labshake search
Citations for BMG Labtech :
101 - 150 of 154 citations for 7 trifluoromethyl 1 2 3 4 tetrahydro 5 nitroisoquinoline hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... Lysates (or lysis buffer alone as a background control) were incubated for 2 h at 37 °C before reading on a fluorescence plate reader (BMG LABTECH) at excitation 360 nm ...
-
bioRxiv - Cancer Biology 2022Quote: ... A 2-hour incubation was used and fluorescence (excitation 560, emission 590O was measured via FLUOstar Omega plate reader (BMG Labtech). Anoikis resistance for each condition was calculated by creating a ratio of the viability of the low-adhesion plate challenged cells to the positive control normal-adhesion plated cells for cells from each treatment condition ...
-
bioRxiv - Cell Biology 2022Quote: ... CCK-8 solution for 2 h and the absorption was recorded at 450nm by using a microplate reader (POLARstar Omega, BMG LABTECH) according to the manufacturer’s protocol.
-
bioRxiv - Immunology 2023Quote: ... fluorescence (485nm excitation, 535nm emission) was measured every 2 minutes for 60 minutes total on the CLARIOstar plate reader (BMG Labtech). Baseline measurements were determined by incubation of liposomes with LUV buffer alone ...
-
bioRxiv - Biochemistry 2024Quote: ... followed by incubation in the dark at room temperature for 2 min before measuring luminescence in a POLARstar OMEGA plate reader (BMG Labtech). Measurements and data processing were done as described in “BRET measurements in HEK293T cells” except that measurements were taken every 0.96 s (with a signal integration time of 0.96 s) ...
-
bioRxiv - Biochemistry 2024Quote: ... followed by incubation in the dark for 2 min at room temperature before measuring luminescence in a POLARstar OMEGA plate reader (BMG Labtech). Measurements and data processing were done as described in “BRET measurements in HEK293T cells” except that measurements with Gαi3/Gβγ ONEGO were made every 0.48 s (with a signal integration time of 0.48 s).
-
bioRxiv - Cell Biology 2024Quote: ... and Calcium Green-5N fluorescence (excitation 506 nm, emission 531 nm) was monitored every 2 s at RT using a CLARIOstar microplate reader (BMG Labtech) after injection of 40 µM CaCl2 ...
-
bioRxiv - Microbiology 2024Quote: ... The translation of the reporter mRNA was monitored every 2 min by measuring YFP fluorescence was measured on a CLARIOstar plate reader (BMG Labtech).
-
Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The solution was centrifuged 16,000g for 10 minutes at 4°C and the supernatant containing the mitochondrial or cell lysate proteins quantified (Bio-Rad DC Protein Assay, BMG Labtech POLARstar Omega plate reader).
-
bioRxiv - Biochemistry 2024Quote: ... ThT fluorescence was recorded every 5 minutes for 16 h on a FLUOstar Omega plate-reader (BMG LABTECH, excitation: 440 nm, emission: 482 nm). Background ThT fluorescence of chaperones and buffer was subtracted and ThT fluorescence was normalised to the fluorescence intensity of the first time point (t = 0 min) ...
-
bioRxiv - Cancer Biology 2020Quote: ... The plate was incubated at 37°C for 2 hours before the absorbance is measured at 490nm using a POLARstar OPTIMA plate reader (BMG Labtech, UK). Readings were blank corrected by subtracting cell free controls and data normalised to untreated control.
-
bioRxiv - Biochemistry 2020Quote: ... The fluorescent intensity signal was measured at 37 °C in kinetic mode with 2 min intervals using CLARIO star microplate reader (BMG LABTECH, Germany) at excitation/emission wavelengths of 530/590 nm ...
-
bioRxiv - Immunology 2020Quote: ... and the cells were lysed with 2% SDS before the absorbance at 550 nm was measured on FLUOstar OMEGA microplate reader (BMG Labtech, UK). Non-coated wells were also seeded and adhesion results were compared between the two cell genotypes ...
-
bioRxiv - Microbiology 2021Quote: ... The β-lactamase cleavage of the green substrate to the blue product was measured by optical density every 15 min for a 2-hour period using a CLARIOstar (BMG Labtech, Ortenberg, Germany) fluorescence reader with excitation at 405 nm and blue and green emission detected at 445 nm and 520 nm respectively ...
-
bioRxiv - Neuroscience 2024Quote: ... Samples were incubated with the antibodies for 2 h at room temperature and then read using a PheraStar FS multi-mode plate reader (BMG Labtech, Ortenberg, Germany). Insulin concentrations (in ng/mL ...
-
bioRxiv - Biochemistry 2021Quote: ... Thioflavin T (ThT) fluorescence was recorded every 5 minutes for 16 hours on a FLUOstar Omega plate-reader (BMG LABTECH, excitation: 440 nm, emission: 482 nm) to monitor the disaggregation of αSyn amyloid fibres ...
-
bioRxiv - Microbiology 2021Quote: ... Assays were performed in triplicate at 30°C and fluorescence intensity was measured at wavelengths of 485 and 540 nm every 1 minute for 1 hour using a FLUOstar Omega plate reader (BMG Labtech). Vmax was then calculated.
-
bioRxiv - Cell Biology 2019Quote: ... Clarified supernatants were mixed 1:1 with ONE-GLO luciferase reagent and luminescence was analysed on a CLARIOstar microplate reader (BMG Labtech). To normalise the data ...
-
bioRxiv - Microbiology 2023Quote: ... Assays were performed in triplicate at 30°C and fluorescence intensity was measured at wavelengths of 485 nm and 540 nm every 1 minute for 1 hour using a FLUOstar Omega plate reader (BMG Labtech). Maximum enzymatic activity (Vmax ...
-
bioRxiv - Cell Biology 2021Quote: ... The two components were combined 1:1 in a final volume of 20 µL in and the reaction monitored in a Pherastar plate reader (BMG LABTECH GmbH, Germany) using excitation at 485 nm (±10 nm ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by luminescent measurement of caspase-1 activity with a FLUOstar OPTIMA reader (BMG LABTECH).
-
bioRxiv - Microbiology 2022Quote: ... The reaction was measured continuously for 1 h in a CLARIOStar plate reader (BMG Labtech) at OD420 and OD550 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by luminescent measurement of caspase-1 activity with a FLUOstar OPTIM reader (BMG LABTECH).
-
bioRxiv - Microbiology 2024Quote: ... in 1-mL cuvettes at 600nm or in a SpectroStar Nano plate reader (BMG LABTECH) at 540nm in sterile 96-well CELLSTAR® plates sealed with a sterile transparent film ...
-
bioRxiv - Microbiology 2021Quote: ... 1% SDS was added to wells and staining intensity was measured using FLUOstar Omega (BMG Labtech). Percentage cell survival was determined by comparing intensity of staining to an uninfected wells ...
-
bioRxiv - Biochemistry 2022Quote: ... FITC fluorescence was measured every 1 min during 60–90min in a microplate fluorescence reader (Clariostar, BMG Labtech). The excitation and emission wavelengths were set at 495nm and 521nm ...
-
bioRxiv - Microbiology 2021Quote: ... which was measured using an integration time of 1 sec/well by the CLARIOstar microplate reader (BMG Labtech). GSH levels were calculated based on GSH standard curves ...
-
bioRxiv - Genetics 2023Quote: ... Fluorescence was measured at 1 min intervals for 100 min using a FLUOstar Galaxy microplate reader (BMG Labtech) at excitation and emission wavelengths of 390 nm and 460 nm respectively.
-
bioRxiv - Molecular Biology 2022Quote: ... All experiments were performed on a CLARIOstar Plus plate reader at 37°C with 1 mM ATP (BMG Labtech).
-
bioRxiv - Immunology 2021Quote: ... The developing reaction was stopped by adding 50 μl 1 M H2SO4 and absorbance was measured at 450 nm with an ELISA microplate reader (FluoStar Omega 5.11, BMG Labtech) with Omega MARS software for analysis ...
-
bioRxiv - Bioengineering 2020Quote: ... RNA purity was confirmed with 260/280 nm absorbance ratio > 1.7 and 260/230 nm absorbance ratio > 1 (BMG LABTECH CLARIOstar monochromatic microplate reader) ...
-
bioRxiv - Microbiology 2023Quote: ... Resorufin fluorescence was monitored at the 1- hr interval for 6 hours using the CLARIOstar Plus (BMG Labtech, Ortenberg, Germany) microplate reader (excitation = 530 nm ...
-
bioRxiv - Immunology 2022Quote: ... The developing reaction was stopped by adding 50 μl of 1 M H2SO4 and absorbance was measured at 450 nm with an ELISA microplate reader (FluoStar Omega, BMG Labtech) with Omega and Omega MARS software for analysis ...
-
bioRxiv - Neuroscience 2019Quote: ... 5% CO2 for 1 hour and viability was assessed by fluorescence reading (Exc 390/ Em 505 nm) at the PHERAstar (BMG Labtech). Ten μL of Steady-Glo Luciferase Assay System were subsequently added to each well ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Enzyme serial dilutions were added in duplicate to 1 mM ABTS and ABTS oxidation was monitored on a PHERAStar FS plate reader (BMG Labtech) at 420 nm at 25 °C ...
-
bioRxiv - Plant Biology 2020Quote: ... leaf samples were excited in the range of 360–496 nm with a step width of 1 nm and the emission was collected at 520 nm using a CLARIOstar plate reader (BMG Labtech). Non-fluorescent samples of each genotype and treatment were used to subtract the basal background fluorescence.
-
bioRxiv - Immunology 2021Quote: ... The developing reaction was stopped by adding 50 μl of 1 M H2SO4 and absorbance was measured at 450 nm with an ELISA microplate reader (FluoStar Omega, BMG Labtech) with Omega and Omega MARS software for analysis ...
-
bioRxiv - Neuroscience 2020Quote: ... The activity was measured following the increase in absorbance at 388 nm due to PLP formation (extinction coefficient of 4900 M-1cm-1) in a CLARIOstar (BMG LABTECH) microplate reader ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were pre-incubated with PF 06882961 or OWL-833 for 10 min prior to assessment of BRET signal between Nluc-hGLP-1R and AF568-GLP-1 This was assessed using a PHERAstar (BMG LabTech) at 10 sec intervals (25 °C) ...
-
bioRxiv - Microbiology 2020Quote: ... and monitoring the absorbance increase (AcSCh conversion or BcSCh conversion) over 1 hour at 405 nm in a Polarstar Omega microplate reader (BMG Labtech). Parasites cultured with naïve IgG served as a negative control ...
-
bioRxiv - Immunology 2022Quote: ... then the developing reaction was stopped by adding 50 μl 1 M H2SO4 and absorbance was measured at 450 nm with an ELISA microplate reader (FluoStar Omega, BMG Labtech) with Omega and Omega MARS software for analysis ...
-
bioRxiv - Plant Biology 2021Quote: ... Luminescence was measured at 1 s intervals in the dark from subjective dawn using orbital scan mode in a LUMIstar Omega plate reader (BMG Labtech) and 50 µl of DMSO or chemicals were applied with an injector at 30 s for final concentration of 10 µM DPI or 25 µM PI ...
-
bioRxiv - Cell Biology 2021Quote: ... and the fluorescence intensity measured at Ex/Em 480/520nm (ZsProSensor-1/GFP) and 360/460nm (Nuclei/DAPI) using CLARIOstar Plus (BMG Labtech). The GFP-to-DAPI was then determined and the inhibition of the proteasome expressed as a percentage of controls (untreated cells ...
-
bioRxiv - Immunology 2022Quote: ... The developing reaction was stopped by adding 50 μl of 1 M H2SO4 and absorbance was measured at 450 nm with an ELISA microplate reader (FluoStar Omega, BMG Labtech) with Omega and Omega MARS software for analysis ...
-
bioRxiv - Biochemistry 2020Quote: The absorbance spectra of 200 μl of 600-800 μM purified YecA or YchJ in buffer 1 were determined from 300-600 nm using a CLARIOstar plate reader (BMG Labtech) using UV-clear flat bottomed 96-well plates (Greiner) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The increase in absorbance at 410 nm was measured at 1 min intervals for 15 min at room temperature using a FLUOstar Optima microplate reader (BMG Labtech). The change in absorbance against time due to the production of 5-thio-2-nitro-benzoic acid and its extinction coefficient was utilized to calculate the acetylcholinesterase activity (μmoles/min) ...
-
bioRxiv - Biochemistry 2021Quote: ... were recorded with a 70 μs delay to reduce background fluorescence and the reaction was followed over 1 h by recording 60 technical replicates of each data point using a PHERAstar FS microplate reader (BMG Labtech). The TR-FRET signal of each data point was extracted by calculating the 520/490nm ratio ...
-
bioRxiv - Immunology 2021Quote: ... then the developing reaction was stopped by adding 50 μl 1 M H2SO4 and absorbance was measured at 450 nm with an ELISA microplate reader (FluoStar Omega, BMG Labtech) with Omega and Omega MARS software for analysis ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The fluorescence emission spectra of all samples were recorded from 458 nm to 600 nm with 1 nm increments at the excitation wavelength of 433 nm (± 10 nm) on a CLARIOstar Plus plate reader (BMG LABTECH). The spectra were smoothed by using a 7-point moving average ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Plates were centrifuged at 1000 x g for 1 min and fluorescence polarization was measured with a PHERAstar plate reader (BMG Labtech) at 25°C ...