Labshake search
Citations for BMG Labtech :
1051 - 1100 of 1148 citations for Allopregnanolone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and HgCl2-preserved and frozen (−20°C) subsamples in the home lab after thawing using a microtiter plate reader (FLUOstar Optima, BMG Labtech, Germany) following established procedures for N oxides (NOx)34 ...
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bioRxiv - Biochemistry 2022Quote: ... After addition of substrate reaction was measured at excitation/emission wavelength 340/420 nm on a plate reader (Clariostar, BMG Labtech, Germany). For estimation of first order rate constants of kcat/Km substrate concentrations from 0.8 to 83 μM were used ...
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bioRxiv - Biochemistry 2022Quote: ... was loaded into a 96 well full-area plate (non-binding, clear bottomed) and incubated at 37 °C for ∼ 140 h in a CLARIOstar Plus microplate reader (BMG Labtech, Germany). Aggregation was promoted through linear shaking (300 rpm ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and Lumi4™-Tb cryptate conjugated anti-IP1 antibody (emission at 620 nm) was detected using a CLARIOstar plate reader (BMG Labtech). Results were calculated from the 665/620 nm ratio and normalised to the maximum response stimulated by ACh.
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bioRxiv - Plant Biology 2019Quote: The UV-visible spectra of affinity purified recombinant DGK4 (200 µg/mL) was recorded on a PHERAstar FS micro-plate reader (BMG Labtech, USA). The heme environment of DGK4 was characterized by the addition of a reducing agent ...
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bioRxiv - Cancer Biology 2020Quote: ... The plate was incubated at 37°C for 2 hours before the absorbance is measured at 490nm using a POLARstar OPTIMA plate reader (BMG Labtech, UK). Readings were blank corrected by subtracting cell free controls and data normalised to untreated control.
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bioRxiv - Biochemistry 2020Quote: ... and β-NADH absorbance at 340 nm (A340) was measured over time at 25°C with a Clariostar plate reader (BMG Labtech). Linear regression analysis was performed with Prism 8 (GraphPad ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... we measured the kinetic absorbance at 25 °C every 76 s for 100 cycles using a BMG Fluorostar Omega plate reader at 595 nm (BMG LABTECH, UK). Three different sources of data ...
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bioRxiv - Plant Biology 2019Quote: ... Rehydration was started during the measurement by injection of water by the built-in reagent injectors of a CLARIOstar plate reader (BMG LABTECH GmbH), while maintaining a constant temperature of 25 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorescent intensity (parallel and perpendicular polarization) of each reaction were measured using a ClarioStar Plus FP plate reader (BMG Labtech, Ortenberg, Germany) and fluorescent anisotropy values of each protein titration were calculated ...
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bioRxiv - Biophysics 2021Quote: ... The kinetics of amyloid fibril formation were monitored at 37°C either under continuous shaking (600 rpm) or under quiescent conditions by measuring ThT fluorescence intensity through the bottom of the plate using a FLUOstar (BMG LABTECH, Germany) microplate reader (readings were taken every 150 or 300 seconds) ...
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bioRxiv - Cancer Biology 2021Quote: ... and RNA purity was evaluated by 260/230 nm and 260/280 nm ratios using a CLARIOstar plate reader (BMG LABTECH, Germany). cDNA was synthesised from 2 μg RNA using the GoScriptTM Reverse Transcription kit (Promega ...
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bioRxiv - Biophysics 2024Quote: ... The kinetics of amyloid fibril formation were monitored at 37°C under continuous shaking (300 rpm) conditions by measuring ThT fluorescence intensity through the bottom of the plate using a PHERAstar (BMG LABTECH, Germany) microplate reader (readings were taken every 150 seconds) ...
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bioRxiv - Cell Biology 2024Quote: ... Cell viability was determined upon addition of 25 µl CellTiter-Glo® reagent in accordance with manufacturer’s instructions and luminescence was measured using a ClarioSTAR Plus plate reader (BMG Labtech, Germany).
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bioRxiv - Microbiology 2024Quote: ... Absorbance at 450Dnm (mAb binding intensities) was measured with a SPECTROstar® Nano absorbance plate reader using the MARS software (BMG Labtech). Fucan from Laminaria (Glycomix ...
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bioRxiv - Biophysics 2023Quote: ... Luminescence was read on a CLARIOstar plate reader and data analyzed using MARS software (both reader and software, BMG Labtech, Ortenberg, GER). Data was further processed using GraphPad-Prism software.
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bioRxiv - Biochemistry 2023Quote: ... which converts resazurin into resorufin (excitation 550 nm, emission 585 nm) that was quantitatively analyzed by plate reader (CLARIOstar Plus, BMG Labtech, German).
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bioRxiv - Biochemistry 2023Quote: ... which converts resazurin into resorufin (excitation 550 nm, emission 585 nm) that was quantitatively analyzed by plate reader (CLARIOstar Plus, BMG Labtech, German). The concentrations of recombinant proteins were determined by Pierce™ Rapid Gold BCA Protein Assay Kit (Cat#A53225 ...
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bioRxiv - Biophysics 2023Quote: A calibration curve was generated to correct raw absorbance readings collected outside the linear range of the plate-reader (POLARstar Omega, BMG Labtech, Germany) used in growth assays ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Protein concentration was determined by BCA assay (Pierce) and the absorbance at 562 nm measured by spectrophotometry on a plate reader (BMG Labtech PHERastar). Samples were separated by SDS-PAGE using 20 μg of protein per well of NuPAGE Novex 4-12% BIS-TRIS gels (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The cells were pelleted and the remaining cytochrome C in the supernatant was measured spectrophotometrically at OD410 using a FLUOstar OMEGA plate reader (BMG Labtech, UK).
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bioRxiv - Neuroscience 2023Quote: ... ThT fluorescence was monitored with bottom optics at λex = 448 nm and λem = 482 nm in a fluorescence plate reader with orbital averaging on 3 mm (Clariostar or Polarstar Optima, BMG labtech, GE). Prior to measurement start ...
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bioRxiv - Neuroscience 2023Quote: ... The MTT formazan product was quantified by measuring the absorbance at 570 nm corrected by subtraction of the absorbance at 660 nm in a FluoroStar Optima plate reader (BMG labtech, GE). All results were normalized to untreated cells grown in medium only ...
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bioRxiv - Cell Biology 2023Quote: ... Fluorescence per well was measured in a 4×4 matrix (1400 gain) using a FLUOstar OPTIMA plate reader (BMG Labtech, Ortenberg, Germany) with emission filters at 485 nm and an excitation filter at 520 nm ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 0.5 s integration time and 1 min intervals at 37 °C using a CLARIOstar® Plus Multimode Plate Reader (BMG Labtech, Germany). The final volume in each well was 125 μL ...
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bioRxiv - Molecular Biology 2019Quote: ... The absorbance increase was monitored every 5 min at 405 nm in a Polarstar Omega microplate reader (BMG Labtech). Specific activity was calculated using the initial velocity of the reaction and extinction coefficient of 13,260 M−1 cm−1 for TNB ...
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bioRxiv - Molecular Biology 2024Quote: ... OD600nm was measured every 5 min over 18 h at 37 °C using a SPECTROstar Omega spectrophotometer (BMG Labtech) with shaking before every measurement ...
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bioRxiv - Plant Biology 2020Quote: ... mScarlet-I (target) and Venus (reference) were excited at 560 ± 10 nm and 497 ± 15 nm in a CLARIOstar plate reader (BMG Labtech, Ortenberg, Germany). Emission was recorded at 595 ± 10 nm (mScarlet-I ...
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bioRxiv - Developmental Biology 2019Quote: ... Oxidation of Amplex Red generates a fluorescence signal measurable at 560nm/590nm excitation/emission wavelengths and was detected using a fluorescence plate reader (BMG Labtech, Ortenberg, Germany) using a 96-well format ...
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bioRxiv - Biochemistry 2020Quote: QF peptide cleavage assays were conducted in 96-well black microtiter plates (Scientific Laboratories Supplies Ltd, Wilford, UK) using a Fluostar Omega microplate reader (BMG Labtech, Aylesbury, UK). The activity of ADAMTS-4 and −5 was monitored for 2 h using the fluorescent peptide substrates fluorescein 5(6)-carbonyl -Ala-Glu-Leu-Asn-Gly-Arg-Pro-Ile-Ser-Ile-Ala-Lys-N,N,N0,N0-tetramethyl-6-carboxyrhodamine-NH2 (FAM-AE~LQGRPISIAK-TAMRA ...
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bioRxiv - Biophysics 2021Quote: ... The assay was then initiated by placing the microplate at 37 °C under quiescent conditions in a plate reader (FLUOstar Omega, BMG Labtech, Aylesbury, UK). The ThT fluorescence was measured through the bottom of the plate with a 440 nm excitation filter and a 480 nm emission filter ...
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bioRxiv - Cell Biology 2021Quote: ... The two components were combined 1:1 in a final volume of 20 µL in and the reaction monitored in a Pherastar plate reader (BMG LABTECH GmbH, Germany) using excitation at 485 nm (±10 nm ...
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bioRxiv - Microbiology 2020Quote: ... plates were read to quantify SYBR Green I® fluorescence intensity in each well by a PHERAstar® FSX microplate reader (BMG Labtech) with excitation and emission wavelengths of 485 and 520nm respectively ...
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bioRxiv - Neuroscience 2022Quote: Ligand-binding activity of cell lines co-expressing Orco and ORs was tested via a calcium fluorescence assay using a CLARIOstar Omega plate reader (BMG Labtech, Ortenberg, Germany) according to previously described protocols (Andersson et al ...
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bioRxiv - Physiology 2022Quote: ... cells were incubated with Caspase-Glo® 3/7 reagent at room temperature before recording luminescence with a POLASTAR plate reader (BMG Labtech, Germany).
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bioRxiv - Biophysics 2022Quote: ... emission 535 nm) was measured in parallel and perpendicular to the excitation plane on a PheraStar FS plate reader (BMG Labtech GmbH, Germany) and polarization was calculated ...
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bioRxiv - Biophysics 2022Quote: ... emission 535 nm) was measured in parallel and perpendicular to the excitation plane on a PheraStar FS plate reader (BMG Labtech GmbH, Germany). The measured intensities were reduced by the values of only AcmJRL in TBS ...
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bioRxiv - Immunology 2022Quote: ... Fluorescence intensity at 460 and 530 nm emission and 405 nm excitation was measured by an PHERAstar plate reader (BMG LABTECH, Cary, NC). Data were represented as the ratio of the emission wavelengths (460nm/530nm).
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bioRxiv - Bioengineering 2022Quote: ... Afterwards the supernatants were transferred to 96 well plates and the absorbance was measured at 580 nm in a microplate reader (FLUOstar, BMG LabTech, Offenburg, Germany). A standard curve was prepared by measuring the absorbance for a series of trypan blue solutions at different concentrations ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... followed by addition of detection solution (60 minutes, room temperature) and measurement of chemiluminescence on a CLARIOstar plate reader (BMG Labtech, Ortenberg, Germany).
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bioRxiv - Synthetic Biology 2020Quote: ... The solution underwent centrifugation at 4000 r.p.m for 20 minutes and the top hexane layer was transferred to a shallow 96-well plate and absorbance at 530 nm was determined (BMG LABTECH POLARstar Omega).
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bioRxiv - Bioengineering 2019Quote: ... The concentration of NP solution was determined with fluorescent standard curves generated from known concentrations of as-received Fluorospheres (FLUOstar Omega fluorescence plate reader; BMG Labtech, Ortenberg, Germany). Yields of NPs ranged between 40-60 % ...
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bioRxiv - Microbiology 2021Quote: ... Concentration and quality of extracted DNA was determined with spectrometry absorbance between 230–280 nm detected by a NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies) and OPTIMA fluorescence plate reader (BMG LABTECH, Jena, Germany). Fungal DNA were subsequently amplified by PCR targeting the internal transcribed spacer (ITS2 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were transferred to 384-well plate and emission (530 nm) was measured with excitation wavelengths of 400 and 482 nm using a Clariostar plate reader (multi-chromatic) (BMG Labtech, Ortenberg, Germany). The calculated ratio of 400/482 nm was then plotted using GraphPad Prism 8.0.2 software ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were transferred into a flat-bottom microtiter plate and hemoglobin release was quantified (490 nm, FLUOstar Omega, BMG Labtech, Cary, NC, USA). Values are expressed in percent hemolysis relative to a 100% lysis control ...
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bioRxiv - Immunology 2021Quote: ... The colorimetric reaction was then terminated with 100 µL of 0.3 M sulfuric acid and the absorbance of the yellow resultant product was measured for each sample at 450nm using a plate reader (FLUOstar Omega, BMG Labtech, Ortenberg Germany). Per Bethyl Laboratories protocol ...
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bioRxiv - Immunology 2021Quote: ... An excitation filter of 485nm and an emission filter of 520nm was used to quantitate mean fluorescent intensity using a fluorescent plate reader (FLUOstar Omega, BMG Labtech, Ortenberg, Germany).
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bioRxiv - Cancer Biology 2019Quote: ... The TR-FRET signal (fluorescence emission ratio of 10,000 × 520 nm/490 nm) from each well was collected with a PHERAstar FS plate reader (BMG LABTECH Inc., Cary, NC). The final tested compound concentration was 20 µM and the final DMSO concentration was 0.2% for all wells in the primary screening ...
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bioRxiv - Bioengineering 2022Quote: ... The converted fluorescent products were photometrically quantified at an excitation wavelength of 544 nm and an emission wavelength of 590 nm using a plate reader (FLUOstar, BMG LabTech, Offenburg, Germany). Cells without films were used as negative control ...
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bioRxiv - Molecular Biology 2022Quote: ... Time-resolved fluorescent measurements were carried out at an excitation wavelength of 340 nm and an emission wavelength of 614 nm on a BMG POLARstar plate reader (BMG Labtech, Melbourne, Australia). Binding was performed in at least three independent experiments with triplicate determinations within each assay ...