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Citations for Biotage :
101 - 150 of 168 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Mixed-mode Cation Exchange extractions were performed using Oasis PRiME MCX 1 cc cartridges (Waters Corporation, Milford, MA, USA, Part Number: 186008917) and a positive pressure manifold (Biotage PRESSURE+48). All samples were diluted into an equal volume of loading buffer (200 mM ammonium formate with phosphoric acid to achieve pH 5) ...
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bioRxiv - Neuroscience 2022Quote: Hydrophilic-Lipophilic Balanced reversed-phase extractions were conducted using the Oasis PRiME HLB 1 cc cartridges (Waters Corporation, Milford, MA, USA, Part Number: 186008055) and a positive pressure manifold (Biotage PRESSURE+48). All samples were diluted into an equal volume of 0.1% trifluoroacetic acid (TFA ...
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bioRxiv - Microbiology 2022Quote: ... The upper organic phase (1.5 mL) was transferred to 2 mL centrifuge tubes and dried under a flow of nitrogen (TurboVap LV, Biotage, Uppsala, Sweden). The polar phase was re-extracted with 1.5 mL of MTBE ...
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bioRxiv - Immunology 2022Quote: ... The supernatant containing the extract was transferred to a new tube for evaporation using TurboVap LV (Biotage), resuspended in 3:3:1 (vol/vol/vol ...
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bioRxiv - Biochemistry 2022Quote: ... The transferred samples were dried with gentle heat and nitrogen gas flow (Biotage, Charlotte, NC) followed by reconstitution with 50:50 MeCN:H2O containing 0.2% acetic acid ...
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bioRxiv - Microbiology 2022Quote: ... Samples were then extracted with isolute plates (Biotage, Uppsala, Sweden) and analysed by LC-MS/MS (Acquity with Quattro Premier XE ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and automated with Isolera™ One with UV-Vis detection (Biotage).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Reversed-phase column flash chromatography was done on silica-gel C18 (SNAP KP-C18-HS, 50μ, Biotage) and automated with Isolera™ One with UV-Vis detection (Biotage).
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bioRxiv - Neuroscience 2022Quote: ... combined with 200 μL MeOH:H2O (1:1) and subjected to prefractionation via C18 solid phase extraction (SPE isolute C18, Biotage AB, Uppsala, Sweden). SPE eluates were evaporated to dryness under N2 gas and reconstituted in 50 μL DMSO and 200 μL mobile phase B liquid chromatography buffer (see below).
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bioRxiv - Neuroscience 2022Quote: ... Samples were placed briefly on a TurboVap® (Zymark, Biotage, Uppsala, Sweden) to remove the organic solvent ...
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bioRxiv - Neuroscience 2022Quote: ... Lipid analytes were isolated from supernatants and preconcentrated via either C18 solid phase extraction (isolute C18, Biotage AB, Uppsala, Sweden) for glucosylceramides or strong cation exchange (Oasis MCX ...
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bioRxiv - Immunology 2022Quote: ... paw extracts were dried under nitrogen flow using a TurboVap (Biotage) and reconstituted in 70 μL 25% acetonitrile ...
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bioRxiv - Molecular Biology 2022Quote: ... The lower organic phase containing the lipids was collected and dried down under a nitrogen stream using a TurboVap Classic LV (Biotage) at a flow rate of 0.5l/min for 15min until no visible solution remains ...
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bioRxiv - Developmental Biology 2022Quote: ... and loaded onto a supported liquid extraction cartridge (Isolute, Biotage, Charlotte, NC). After 5 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Fractions were dried into pre-weighed vials using a V10-touch evaporator (Biotage) coupled with a Gilson GX-271 Liquid Handler ...
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bioRxiv - Microbiology 2022Quote: ... Fractions were dried into pre-weighed vials using a V10-touch evaporator (Biotage®) coupled with a Gilson GX-271 Liquid Handler ...
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bioRxiv - Immunology 2022Quote: ... The aqueous phase was transferred to a new tube for evaporation using TurboVap LV (Biotage), washed with 60 μl methanol and dried again ...
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bioRxiv - Plant Biology 2022Quote: ... Peptides were further desalted and purified on Isolute C18 SPE cartridges (Biotage, Sweden) and dried in an Eppendorf concentrator (Eppendorf ...
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bioRxiv - Microbiology 2022Quote: ... This resin was then moved forward into parallel synthesis on a Syro II parallel peptide synthesizer (Biotage) at a 5 mg scale ...
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bioRxiv - Biochemistry 2022Quote: ... mucus (300 µL) and media (150 µL) samples were first dried to completion under a stream of nitrogen (TurboVap 96, Biotage) and resolubilized in 50 µL of lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Flowthroughs were then pooled and dried under a stream of nitrogen (TurboVap 96, Biotage) and peptides were resuspended in 100 µL of 0.1% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... The eluates were brought to dryness using a TurboVap LV evaporation system (Biotage, Uppsala, Sweden) and resuspended in 200 μL methanol/water (50/50 ...
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bioRxiv - Neuroscience 2021Quote: The spike peptides were synthesized with a peptide synthesizer Syro Wave (Biotage, Sweden) using Fmoc solid-phase chemistry ...
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bioRxiv - Neuroscience 2021Quote: ... synthesized with a peptide synthesizer Syro Wave (Biotage, Sweden) using Fmoc solid-phase chemistry ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The synthesis was performed on a Syro I instrument from Biotage (Uppsala, Sweden). Coupling conditions were room temperature for 2 × 120 min using 5.2 eq amino acids ...
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bioRxiv - Synthetic Biology 2021Quote: Non-labeled quantification tags were synthesized by Fluorenylmethyloxycarbonyl (Fmoc) chemistry using a peptide synthesizer SyroWave (Biotage, Uppsala, Sweden). Fmoc amino acids were purchased from Watanabe Chemical Industries and contained the following side chain protecting groups ...
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bioRxiv - Plant Biology 2021Quote: ... HAAs were then isolated from digested mixture using flash chromatography on a Biotage (Stockholm, Sweden) Isorela One instrument with a SNAP Ultra C18 12g column (Biotage) using an acetonitrile/water gradient at 12 mL/min flow rate ...
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bioRxiv - Biochemistry 2021Quote: Peptides were synthesized by Fmoc solid phase peptide synthesis on a Syro II peptide synthesizer (Biotage) at ambient temperature and atmosphere on a 12.5 μM using either pre-loaded Wang resin or Rink amide resin (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... Flash chromatography over silica gel column was performed using an Isolera One flash chromatography system from Biotage, Uppsala ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: The preliminary purification runs were performed on ISOLERA ONE (Biotage AB, Uppsala, Sweden) instrument equipped with UV-Vis detector (detection wavelength 254 nm with 15 mAU as threshold) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The column used was a BIOTAGE SNAP (Biotage AB, Uppsala, Sweden) Ultra (10 g ...
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bioRxiv - Physiology 2021Quote: Chicken NPGM was synthesized using fluorenylmethyloxycarbonyl (Fmoc) and a peptide synthesizer (Syro Wave; Biotage, Uppsala, Sweden) according to our previous method (Masuda et al. ...
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bioRxiv - Physiology 2021Quote: ... was synthesized by microwave-assisted solid-phase peptide synthesis using an automated peptide synthesizer (Initiator+ Alstra; Biotage, Uppsala, Sweden) as previously described [21].
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bioRxiv - Immunology 2021Quote: ... The samples were then subjected to solid phase extraction using Isolute C18 columns (Biotage). Lipid mediators were eluted from the column following addition of methyl formate and were then concentrated under N2 gas and resuspended in methanol:water (50:50) ...
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bioRxiv - Microbiology 2021Quote: ... The resulting material was dissolved in MeOH and applied to a 12 g C18 flash chromatography column (Biotage), pre-equilibrated in 70% MeOH ...
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bioRxiv - Biochemistry 2021Quote: Untagged peptides were synthesized at 25 μM scale on NovaPEG Rink Amide resin (0.53 mmol/g) using Fmoc-based chemistry on a Syro I peptide synthesizer (Biotage). Fmoc-protected amino acid (6 eq.) ...
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bioRxiv - Biophysics 2021Quote: ... The crude peptides were purified by flash chromatography on Isolera Prime chromatographer (Biotage, Uppsale, Sweden) using a SNAP Cartridge KP-C18-HS 12g or preparative RP-HPLC on a Phenomenex C18 column (22.1x250 mm ...
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bioRxiv - Neuroscience 2021Quote: Solid phase extraction was carried out on a Pressure+ 48 (positive pressure Manifold, Biotage) at ≥ 2 psi ...
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bioRxiv - Immunology 2021Quote: ... was synthesized using Fmoc solid-phase peptide synthesis on a peptide synthesizer (Syrowave, Biotage). The purity of the peptide was 98% as assessed by analytical reversed-phase HPLC-MS ...
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bioRxiv - Biochemistry 2021Quote: Macrocyclic peptides were synthesized by standard Fmoc solid-phase peptide synthesis (SPPS) using a Syro Wave automated peptide synthesizer (Biotage). The resulting peptide–resin (25 μmol scale ...
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bioRxiv - Microbiology 2021Quote: ... Trityl-ChemMatrix resin was purchased from Biotage (Charlotte, NC). PyBOP was purchased from Oakwood Chemical (Estill ...
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bioRxiv - Microbiology 2021Quote: ... on a hydrazine derivatized trityl-ChemMatrix resin (Biotage). Linear hydrazide peptides were oxidized with NaNO2 ...
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bioRxiv - Biochemistry 2021Quote: ... Peptide synthesis resins (Trityl-OH ChemMatrix and Rink Amide ChemMatrix) were purchased from Biotage. All analytical reversed-phase HPLC (RP-HPLC ...
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bioRxiv - Biochemistry 2021Quote: ... Volatiles were removed under reduced pressure by rotary evaporation or by using the V-10 solvent evaporator system by Biotage® ...
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bioRxiv - Immunology 2021Quote: ... for 96-well plate cultures or a turbovap (Biotage) for bacterial tube cultures or eppendorf tube lysates ...
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bioRxiv - Genomics 2020Quote: ... Analysis of methylation status was performed using Pyro Q-CpG software (Biotage).
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bioRxiv - Genetics 2020Quote: ... Percent CpG methylation was calculated by Pyro Q-CpG 1.0.9 software (Biotage) using the ratio of C-to-T at each site ...
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bioRxiv - Cancer Biology 2020Quote: ... peptides were synthesized using standard Fmoc chemistry on microwave-assisted synthesizer (Biotage, Sweden). The peptides were cleaved from the resin ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Version 3.0 (Biotage®); RediSep® Rf columns (Teledyne ISCO™) ...
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bioRxiv - Genetics 2020Quote: ... CpG site-specific methylation was quantified using the PyroMark™ Q96 MD pyrosequencer (Biotage) as previously described (Kazachenka et al ...