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Citations for AmsBio :
1 - 50 of 82 citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Similar results were also obtained using a commercially available pseudotyped SARS-CoV-2 S protein expressing viral particles (Amsbio LLC).
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bioRxiv - Developmental Biology 2020Quote: ... Single cells were resuspended in 40 μL drops of phenol red-free Cultrex RGF BME Type 2 (BME 2, Amsbio, #3533-005-02), and seeded in CellCarrier-96 Ultra Microplates (PerkinElmer ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were resuspended in ice-cold BME (BME 2 RGF, AMSBIO, 3533-010-02) at a density of 3,000 cells per μl ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor cells were grown as organoids embedded in basement membrane extract (Cultrex BME Type 2, AMSbio) using tumor organoid medium composed of Advanced DMEM/F12 (Gibco) ...
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bioRxiv - Cell Biology 2019Quote: ... 2 × 103 cells were then seeded in each well of a 96-well lipidure plate (Cat.#AMS.51011610, Amsbio) and maintained in GK15 media composed by Glasgow’s MEM (GMEM ...
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bioRxiv - Cancer Biology 2024Quote: ... cell pellet resuspended in 80% growth factor-reduced basement matrix (either Matrigel®, Corning, 356231; or BME-2®, AMSBIO, 3533) and seeded at the concentration of approximately 50,000 cells/mL by depositing at least six 40 μL drops at the bottom of a non-tissue culture treated plate ...
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bioRxiv - Cell Biology 2022Quote: ... before high-pressure freezing the carriers containing the 3D cell cultures were dipped for one minute either directly in Cellbanker 2 cryo-preserving medium (catalog number 11891, AMSBIO, Cambridge -US) or in 20% Ficoll 70.000 MW (catalog number F2878 ...
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bioRxiv - Cancer Biology 2021Quote: Dissociated prostate cells were resuspended in 80% growth factor-reduced basement matrix (either Matrigel®,Corning, 356231; or BME-2®,AMSBIO, 3533) and seeded at the concentration of approximately 50,000 cells/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... Type 2 RGF PathClear (Amsbio 3533-010-02). Organoids were cultured in IntesticultTM Organoid Growth media (06005 ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μM synthetic retinoid Ec23 (Amsbio #AMS.SRP002-2), 500 nM Smoothened agonist SAG (Millipore #5666600 ...
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bioRxiv - Biochemistry 2021Quote: ... 1-2 mg/mL Zymolyase (AMSBIO, 120493-1)] for lysis at 30°C for 15 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Type 2 RGF PathClear (Amsbio 3533-010-02). In brief ...
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bioRxiv - Developmental Biology 2022Quote: ... Type 2 RGF PathClear (Amsbio, Abbingdon, UK, 3533-010-02). Briefly ...
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bioRxiv - Biophysics 2024Quote: ... reconstituted in CELLBANKER 2® freezing media (Amsbio LLC, 11891) and kept in liquid nitrogen for future use ...
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bioRxiv - Neuroscience 2023Quote: ... 2-µm diameter electrospun microfibers (Amsbio, Mimetix aligned 12-well inserts) that mimic the shape of axons were coated with poly-D-lysine and immunopanned OPCs were seeded onto the microfibers in differentiation media.
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bioRxiv - Immunology 2023Quote: ... After the PBMC suspension was resuspended in CELLBANKER (Amsbio CELLBANKER 2, 11914), the collected PBMCs were immediately cryopreserved and stored in a freezing container (Nalgene Mr ...
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bioRxiv - Cell Biology 2023Quote: ... 50 mM 2-mercaptoethanol) and incubated with 50 mg/mL zymolyase 100T (AMSBIO) at 37°C for 30-60 minutes until the mixture became clear ...
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bioRxiv - Plant Biology 2022Quote: ... coli cells (Amsbio) were transformed by heat shock at 42 °C ...
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bioRxiv - Developmental Biology 2022Quote: 96-well plates with parallel-aligned 2-4 μm electrospun fibers (AMS.TECL-005-8X, AMSBio) were washed with 70% ethanol followed by coating with PO for at least 1 hour at 37°C and laminin for at least 3 hours at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting single cell suspension was cryopreserved in Cell Banker 1 (Amsbio) for long-term storage ...
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bioRxiv - Cancer Biology 2023Quote: ... Healthy CD34+ cells (Amsbio) were cultured in SFEMII with StemSpan CD34 Expansion Supplement (Stem Cell Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM β-mercaptoethanol) and spheroplasted with 2 mg/ml Zymolyase 20T (Amsbio LLC, 120491-1) at 23 °C for 45 min ...
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bioRxiv - Microbiology 2023Quote: ... 75 cm2 flasks were incubated in TS medium containing 2 μg/mL iMatrix-55 (AMSBIO, Abingdon, UK) for 10 minutes at 37°C in 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant FIBCD1-FReD18 was added to each well in a constant concentration of 100 ng/ml in TBS containing 0.05% Tween and 5mM CaCl2 together with 2-fold dilutions of CS-4S and CS-6S (Amsbio). After incubation overnight at 4°C and washing ...
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bioRxiv - Microbiology 2020Quote: ... overnight at 22 °C followed with two bead purification steps with 2 volumes of MagSi-NGSPREP Plus beads (AMSBIO). A PCR enrichment test was performed in order to determine the number of PCR cycles that are nesessery for each pool followed by a bead purification step and a QC with DNA HS kit (Agilent) ...
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bioRxiv - Microbiology 2020Quote: ... the reactions were neutralized with Acetic acid and were purified with 2 volumes of MagSi-NGSPREP Plus beads (AMSBIO). A second 3Tr3 adaptor were ligated to the cDNA using T4 RNA Ligase (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were passaged to mitomycin-C inactivated CF-1 MEFs (3×103 cells/cm2; GSC-6101G, Amsbio) in media consisting of DMEM/F12 with 20% Knockout Serum Replacement (10828010 ...
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bioRxiv - Genetics 2023Quote: ... We partially digested the cell walls of the fixed yeast cells with Zymolyase 20T (Amsbio, #120491-1) to enable lysis in the Chromium device from 10x Genomics ...
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bioRxiv - Physiology 2022Quote: Monolayers of HCAEC grown in Transwell membranes were stained for heparan sulfate using mouse monoclonal anti-heparan sulfate antibody (10E4 epitope, 1:100 in 2% goat serum, AMSBIO) following previously established protocols.[31] Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... organoids were suspended in 20μl 80:20 ECM:Media droplets with a protein concentration of between 6.4-9.6mg/ml (BME-2, Amsbio 3533-005-02) on pre-warmed 6-well plates using previously published media recipes2,10 ...
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bioRxiv - Developmental Biology 2020Quote: ... were differentiated three times toward skeletal muscle lineage using commercial media designed from Caron’s work (23) (Skeletal Muscle Induction medium SKM01, Myoblast Cell Culture Medium SKM02, Myotube Cell Culture Medium SKM03, AMSbio). This protocol is a 2D directed differentiation that uses 3 consecutive defined media (SKM01 from day 0 to 10 ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were counted with a hemocytometer and immediately used for luciferase assay or cryopreserved in Cell Banker medium (Amsbio) at a density of 4×106 cells/mL.
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bioRxiv - Cancer Biology 2020Quote: ... All lines were cultured in drops of Cultrex PathClear Reduced Growth Factor Basement Membrane Extract Type 2 (Amsbio, 3533-005-02) in murine small intestinal organoids medium containing advanced DMEM/F12 medium (adDMEM/F12 ...
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bioRxiv - Cell Biology 2023Quote: Trophoblast Stem Cells (TSC) (CT29, Acquired from RIKEN Cell Bank) were maintained in a self-renewing state on iMatrix511 (Amsbio) coated plates in TS Complete media ...
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bioRxiv - Cell Biology 2024Quote: ... hiPSC-derived skeletal myoblasts were seeded and differentiated towards myotubes using commercial media (Myoblast Cell Culture Medium SKM02, Myotube Cell Culture Medium SKM03, AMSbio). Myoblasts were thawed and seeded at 8 000 cells/cm² in SKM02 for amplification ...
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bioRxiv - Cancer Biology 2022Quote: ... and mixed at a 1:1 ratio with Cultrex® PathClear Reduced Growth Factors Basement Membrane Extract (BME) Type 2 (Amsbio, Abingdon, England). The BME-cell suspension was seeded as 30 μL drops on prewarmed 24-wells suspension culture plates (Greiner Bio-One ...
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Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... resuspended with Cell banker Type I (Amsbio, ZNQ CB011) and cryopreserved in liquid nitrogen until use.
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bioRxiv - Genetics 2022Quote: ... cells were immersed in Cellbanker 1 (Amsbio, catalogue #11888) and frozen in liquid nitrogen ...
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bioRxiv - Developmental Biology 2022Quote: Parallel-aligned 2-4 μm electrospun fibers fitted to 12-well plate inserts were placed in 12 well plates (AMSBio, AMS-TECL-006-4x). Prior to use ...
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bioRxiv - Developmental Biology 2021Quote: ... samples were incubated in FACSmax cell dissociation solution (Amsbio, T200100) with 30U/mL Papain (Sigma ...
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bioRxiv - Physiology 2022Quote: ... dead cells and debris were removed using ClioCell nanoparticles (Amsbio) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... and dead cells were removed using magnetic beads (AMSBIO, CB002) to ensure cell viability >80% ...
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bioRxiv - Cancer Biology 2020Quote: ... organoid cells were resuspended in basement membrane matrix BME (Amsbio) and plated as 15 μl droplets ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were stored at -80° C in Cellbanker1 (Amsbio LLC), and thawed >1 week prior to any imaging.
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bioRxiv - Developmental Biology 2023Quote: ... Cells were maintained in Stemfit Basic04 (Amsbio, SFB-504-CT) on Geltrex (Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... To induce 3- germ-layer differentiation iPSCs were dissociated into single cells using TrypLE Express and plated in an ultra-low-binding 96-well plate (9000 cells/well; Amsbio, lipidure-coat plate A-U96) in Pluripro-medium (PP ...
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bioRxiv - Developmental Biology 2021Quote: ... the medium was changed to myotube cell culture medium (SKM03, Amsbio) and freshly changed every 3 days for more than 6 days ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were spheroplasted using 10 mg zymolyase (100T, AMSBIO, 120493-1) per 100 OD660 cells ...
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bioRxiv - Biochemistry 2023Quote: ... Cell walls were digested with zymolase 100-T (120493-1; Amsbio) at a ratio of 75 uL zymolase (5 mg/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... and maintained for 6 days in myoblast cell culture medium (SKM02, Amsbio). At day 12 ...