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Citations for AmsBio :
51 - 87 of 87 citations for 7 Methyl 1 5 dioxo 1 2 3 5 tetrahydro indolizine 6 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... cell walls were digested for 30 minutes with 250 μg/ml zymolyase 100T (Amsbio; 120493-1) in zymolyase buffer (1.2M Sorbitol ...
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bioRxiv - Molecular Biology 2022Quote: ... UIR and UUO whole kidney lysates (n=4-6 per group) with RNA Stat-60 extraction reagent (Amsbio, CS-111) and purified using the GeneJET RNA purification kit (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... Single cells were resuspended in 40 μL drops of phenol red-free Cultrex RGF BME Type 2 (BME 2, Amsbio, #3533-005-02), and seeded in CellCarrier-96 Ultra Microplates (PerkinElmer ...
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bioRxiv - Genetics 2023Quote: ... We partially digested the cell walls of the fixed yeast cells with Zymolyase 20T (Amsbio, #120491-1) to enable lysis in the Chromium device from 10x Genomics ...
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bioRxiv - Developmental Biology 2022Quote: ... Type 2 RGF PathClear (Amsbio, Abbingdon, UK, 3533-010-02). Briefly ...
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bioRxiv - Biophysics 2024Quote: ... reconstituted in CELLBANKER 2® freezing media (Amsbio LLC, 11891) and kept in liquid nitrogen for future use ...
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bioRxiv - Microbiology 2020Quote: ... then heparan sulfate was stained using 1:30 of F58-10E4 as primary antibody (AMSBIO, cat#370255-S) in PBS 3% BSA for 30 to 40 minutes on ice then washed with PBS 1% FBS and incubated with 1:30 anti-mouse Alexa Fluor 594 (Thermo ...
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bioRxiv - Cell Biology 2021Quote: ... and 7,000 cells were seeded into each well of Lipidure-coated 96-well U-bottomed plate (Amsbio AMS LCP-A-U96-6) and cultured in mTeSR-1 medium supplemented with 10 uM ROCK inhibitor Y-27632 (Chemdea ...
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bioRxiv - Neuroscience 2023Quote: ... 2-µm diameter electrospun microfibers (Amsbio, Mimetix aligned 12-well inserts) that mimic the shape of axons were coated with poly-D-lysine and immunopanned OPCs were seeded onto the microfibers in differentiation media.
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bioRxiv - Pharmacology and Toxicology 2019Quote: Arrays of freshly frozen human colorectal tumor (37) and healthy colon tissues (3) were obtained from Amsbio. A list of the 40 different tissues ...
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bioRxiv - Microbiology 2020Quote: ... then suspended again in 3 mL of spheroplasting buffer containing 100 μg oxalyticase (Zymolyase®-20T, AMSBIO). Mixtures were incubated at 37°C for 1 hr with gentle shaking ...
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bioRxiv - Immunology 2023Quote: ... After the PBMC suspension was resuspended in CELLBANKER (Amsbio CELLBANKER 2, 11914), the collected PBMCs were immediately cryopreserved and stored in a freezing container (Nalgene Mr ...
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bioRxiv - Molecular Biology 2021Quote: ... cell pellets in a 50ml conical centrifuge tube (Falcon) were resuspended in 2mL 1M sorbitol with 0.5 mL of 2.5 mg/mL Zymolyase 100T (Amsbio, 120493-1) and shaken gently (75rpm ...
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bioRxiv - Genetics 2020Quote: ... Each PGC line was in culture for approximately 3 weeks before freezing in aliquots of 50,000 cells resuspended in 125 μl of Stem-Cellbanker (Amsbio) and stored at −150 °C.
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bioRxiv - Cell Biology 2023Quote: ... 50 mM 2-mercaptoethanol) and incubated with 50 mg/mL zymolyase 100T (AMSBIO) at 37°C for 30-60 minutes until the mixture became clear ...
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bioRxiv - Cell Biology 2020Quote: ... followed by spheroplasting for 90 min at 30°C in zymolyase buffer containing 0.43 mg/mL zymolyase 20T (Amsbio 120491-1). The resulting spheroplasts were washed once with 30°C zymolyase buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... tumour cells were transduced with lentiviral expression particles containing either: (1) a firefly luciferase gene with a blasticidin-resistance gene (cells denoted -Luc) (Amsbio, LVP326); (2 ...
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bioRxiv - Neuroscience 2024Quote: ... PNN “stubs” after ABC digestion were identified by staining with mouse anti-chontroitin sulfate (1B5) antibody (1:20, Amsbio, 270431-CS). Secondary antibodies were AlexaFluor 405 goat anti-rabbit for PV or AlexaFluor 594 goat anti-mouse (1:500 ...
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bioRxiv - Genomics 2020Quote: ... cells from overnight cultures were pelleted at 1000g for 3 minutes and resuspended in 50 μl of 300U/ml Zymolyase 100-T (AMSBIO) solution ...
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bioRxiv - Genetics 2023Quote: ... We resuspended 1 ml of the sporulation cultures in 100 μl of water and added 20 μl of 2000 U/ml Zymolyase 20T (Amsbio, #120491-1). The spores were digested for 25 minutes at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were resuspended in ice-cold BME (BME 2 RGF, AMSBIO, 3533-010-02) at a density of 3,000 cells per μl ...
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bioRxiv - Developmental Biology 2022Quote: 96-well plates with parallel-aligned 2-4 μm electrospun fibers (AMS.TECL-005-8X, AMSBio) were washed with 70% ethanol followed by coating with PO for at least 1 hour at 37°C and laminin for at least 3 hours at 37°C ...
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bioRxiv - Genomics 2021Quote: ... ESC were adapted to 2i media (serum-free N2B27 medium supplemented with MEK inhibitor PD0325901 [0.4 μM, Miltenyi Biotec], GSK3β inhibitor CHIR99021 [3 μM, Amsbio], and LIF) for at least four days in gelatin-coated tissue culture plates79 ...
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bioRxiv - Molecular Biology 2024Quote: ... Human embryonic kidney HEK293T (ATCC, CRL-3216), human breast cancer MDA-MB-231 (ATCC, HTB-26) and SK-BR-3 (AMSBIO, Abingdon, UK) cell lines were cultured under standard conditions in DMEM supplemented with 10% Fetal Bovine Serum ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor cells were grown as organoids embedded in basement membrane extract (Cultrex BME Type 2, AMSbio) using tumor organoid medium composed of Advanced DMEM/F12 (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... 75 cm2 flasks were incubated in TS medium containing 2 μg/mL iMatrix-55 (AMSBIO, Abingdon, UK) for 10 minutes at 37°C in 5% CO2 ...
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bioRxiv - Cell Biology 2019Quote: ... 2 × 103 cells were then seeded in each well of a 96-well lipidure plate (Cat.#AMS.51011610, Amsbio) and maintained in GK15 media composed by Glasgow’s MEM (GMEM ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant FIBCD1-FReD18 was added to each well in a constant concentration of 100 ng/ml in TBS containing 0.05% Tween and 5mM CaCl2 together with 2-fold dilutions of CS-4S and CS-6S (Amsbio). After incubation overnight at 4°C and washing ...
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bioRxiv - Microbiology 2020Quote: ... overnight at 22 °C followed with two bead purification steps with 2 volumes of MagSi-NGSPREP Plus beads (AMSBIO). A PCR enrichment test was performed in order to determine the number of PCR cycles that are nesessery for each pool followed by a bead purification step and a QC with DNA HS kit (Agilent) ...
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bioRxiv - Microbiology 2020Quote: ... the reactions were neutralized with Acetic acid and were purified with 2 volumes of MagSi-NGSPREP Plus beads (AMSBIO). A second 3Tr3 adaptor were ligated to the cDNA using T4 RNA Ligase (NEB ...
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bioRxiv - Microbiology 2020Quote: ... Similar results were also obtained using a commercially available pseudotyped SARS-CoV-2 S protein expressing viral particles (Amsbio LLC).
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bioRxiv - Cancer Biology 2021Quote: ... organoids were suspended in 20μl 80:20 ECM:Media droplets with a protein concentration of between 6.4-9.6mg/ml (BME-2, Amsbio 3533-005-02) on pre-warmed 6-well plates using previously published media recipes2,10 ...
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bioRxiv - Cancer Biology 2020Quote: ... All lines were cultured in drops of Cultrex PathClear Reduced Growth Factor Basement Membrane Extract Type 2 (Amsbio, 3533-005-02) in murine small intestinal organoids medium containing advanced DMEM/F12 medium (adDMEM/F12 ...
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bioRxiv - Cancer Biology 2024Quote: ... cell pellet resuspended in 80% growth factor-reduced basement matrix (either Matrigel®, Corning, 356231; or BME-2®, AMSBIO, 3533) and seeded at the concentration of approximately 50,000 cells/mL by depositing at least six 40 μL drops at the bottom of a non-tissue culture treated plate ...
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bioRxiv - Cell Biology 2022Quote: ... before high-pressure freezing the carriers containing the 3D cell cultures were dipped for one minute either directly in Cellbanker 2 cryo-preserving medium (catalog number 11891, AMSBIO, Cambridge -US) or in 20% Ficoll 70.000 MW (catalog number F2878 ...
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bioRxiv - Cancer Biology 2021Quote: Dissociated prostate cells were resuspended in 80% growth factor-reduced basement matrix (either Matrigel®,Corning, 356231; or BME-2®,AMSBIO, 3533) and seeded at the concentration of approximately 50,000 cells/mL ...
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bioRxiv - Developmental Biology 2022Quote: Parallel-aligned 2-4 μm electrospun fibers fitted to 12-well plate inserts were placed in 12 well plates (AMSBio, AMS-TECL-006-4x). Prior to use ...