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Citations for AmsBio :
251 - 290 of 290 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... and then clonal PGCs were cryopreserved in STEM-CELLBANKER (AMSBIO).
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bioRxiv - Genomics 2020Quote: ... cells from overnight cultures were pelleted at 1000g for 3 minutes and resuspended in 50 μl of 300U/ml Zymolyase 100-T (AMSBIO) solution ...
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bioRxiv - Cancer Biology 2020Quote: ... Type 2 RGF PathClear (Amsbio 3533-010-02). Organoids were cultured in IntesticultTM Organoid Growth media (06005 ...
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bioRxiv - Developmental Biology 2020Quote: ... Macrophages were incubated with 3 ug/mL biotinylated Hyaluronan binding protein (Amsbio) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Lentiviral particles were commercially obtained from AMSBIO (Abingdon, UK). Viral transduction of organoids for stable expression of fluorescent markers in hCCOs was performed according to a protocol published by Broutier et al ...
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bioRxiv - Cell Biology 2020Quote: hCCOs were transduced using a third generation lentivector (pLV-Puro-EF1A-H2B/EGFP:T2A:LifeAct/mCherry, vector ID: IK-VB180119-1097haw, custom-made by and commercially obtained from AMSBIO, Abingdon, UK) for stable expression of the fluorescent fusion proteins H2B-eGFP to visualise cell nuclei and LifeAct-mCherry to visualise the F-actin cytoskeleton ...
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bioRxiv - Cell Biology 2020Quote: ... and re-plated in a BMER (AMSBIO cat# 3533-005-02) drop ...
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bioRxiv - Neuroscience 2020Quote: The S1 proteins (Raybiotech, Cat no 230-30161, Peachtree Corners, GA; Amsbio, AMS.S1N-C52H3, Abingdon, UK) were provided by the manufacturer dissolved in phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2020Quote: ... the injection contained 1 μg/mouse of unlabeled S1 (Amsbio), mouse acyl ghrelin (CBio ...
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bioRxiv - Microbiology 2020Quote: ... Similar results were also obtained using a commercially available pseudotyped SARS-CoV-2 S protein expressing viral particles (Amsbio LLC).
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bioRxiv - Bioengineering 2020Quote: ... IVDD was induced via percutaneous intradiscal injection (29-gauge needle) of 1U of C-ABC (Amsbio, Cambridge, MA) in 200 µL of vehicle (sterile 0.1% bovine serum albumin in 1x PBS - Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM β-mercaptoethanol) and spheroplasted with 2 mg/ml Zymolyase 20T (Amsbio LLC, 120491-1) at 23 °C for 45 min ...
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Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasisbioRxiv - Cancer Biology 2020Quote: ... D2A1 and E0771 cells were luciferase transduced with lentiviral expression particles containing a firefly luciferase gene and a blasticidin-resistance gene (Amsbio, LVP326). Where indicated ...
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bioRxiv - Neuroscience 2020Quote: ... RNA was extracted using STAT60 reagent (Amsbio, MA, USA). Complementary DNA from 1 µg of input RNA was generated with the High Capacity cDNA Reverse Transcription Kits (Life Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... overnight at 22 °C followed with two bead purification steps with 2 volumes of MagSi-NGSPREP Plus beads (AMSBIO). A PCR enrichment test was performed in order to determine the number of PCR cycles that are nesessery for each pool followed by a bead purification step and a QC with DNA HS kit (Agilent) ...
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bioRxiv - Microbiology 2020Quote: ... the reactions were neutralized with Acetic acid and were purified with 2 volumes of MagSi-NGSPREP Plus beads (AMSBIO). A second 3Tr3 adaptor were ligated to the cDNA using T4 RNA Ligase (NEB ...
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bioRxiv - Microbiology 2020Quote: ... Barcoded DNA Libraries were purified using magnetic MagSi-NGSPREP Plus beads (AMSBIO) at a 1.8 ratio of beads to sample volume ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-mouse heparan sulfate (10E4 epitope, 1:100, AMSBIO, catalog F58-10E4), rabbit anti-mouse NeuN (1:250 ...
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bioRxiv - Genomics 2020Quote: ... Samples were mixed with 10x Laemlli buffer (42556.01, Amsbio, Cambridge, MA), boiled for 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mL of RNA STAT-60 (Amsbio LLC, Cambridge, MA) was added to each tube ...
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bioRxiv - Neuroscience 2020Quote: ... or in the same solution supplemented with 0.2 U/ml of protease-free chondroitinase ABC (ChABC) from Proteus vulgaris (Amsbio, UK). Next ...
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bioRxiv - Microbiology 2020Quote: Localization of PDGFRβ in formalin-fixed paraffin-embedded normal human skin and liver sections (Amsbio, MA, USA) was performed using a EnVision G2 Doublestain System ...
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bioRxiv - Cancer Biology 2020Quote: ... and human adipose derived stem cells were from Amsbio (Cambridge, MA, USA). Human MSCs were cultured in StemMACS MSC Expansion Medium (Miltenyi Biotec ...
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bioRxiv - Biophysics 2020Quote: ... The production of tau seeds was carried out by incubating 16uM wild-type tau with 1:1 molar ratio of Heparin (AMSbio) for 1 hour at 37°C in 30 mM MOPS pH7.4 ...
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bioRxiv - Cancer Biology 2020Quote: ... BME (Amsbio) was disturbed by pipetting and organoids were disassociated using TrypLE (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... and stored in an equal volume of RNA-Bee (Amsbio, CS-104B) at -80-degree C until proceeding to RNA extraction ...
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bioRxiv - Neuroscience 2020Quote: ... and stored in an equal volume of RNA-Bee (Amsbio, CS-104B) at −80°C until RNA extraction ...
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bioRxiv - Cancer Biology 2020Quote: ... HS was stained using an antibody (370255, Amsbio). Quantification of HA expression of cells in well plates was performed by imaging on a Zeiss Axiovert fluorescent microscope with a 20X objective ...
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bioRxiv - Microbiology 2020Quote: ... then heparan sulfate was stained using 1:30 of F58-10E4 as primary antibody (AMSBIO, cat#370255-S) in PBS 3% BSA for 30 to 40 minutes on ice then washed with PBS 1% FBS and incubated with 1:30 anti-mouse Alexa Fluor 594 (Thermo ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated recombinant human FcRn (Amsbio LLC, Cambridge, MA) was diluted to 5 μg/ml in running buffer - 100 mM Tris pH 6 (adjusted by addition of dry citric acid) ...
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bioRxiv - Cancer Biology 2020Quote: ... All lines were cultured in drops of Cultrex PathClear Reduced Growth Factor Basement Membrane Extract Type 2 (Amsbio, 3533-005-02) in murine small intestinal organoids medium containing advanced DMEM/F12 medium (adDMEM/F12 ...
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bioRxiv - Microbiology 2020Quote: ... then suspended again in 3 mL of spheroplasting buffer containing 100 μg oxalyticase (Zymolyase®-20T, AMSBIO). Mixtures were incubated at 37°C for 1 hr with gentle shaking ...
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bioRxiv - Developmental Biology 2020Quote: ... were differentiated three times toward skeletal muscle lineage using commercial media designed from Caron’s work (23) (Skeletal Muscle Induction medium SKM01, Myoblast Cell Culture Medium SKM02, Myotube Cell Culture Medium SKM03, AMSbio). This protocol is a 2D directed differentiation that uses 3 consecutive defined media (SKM01 from day 0 to 10 ...
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bioRxiv - Immunology 2020Quote: ... InvivoGen) and 6G9 (human:mouse chimeric IgG anti-N; Amsbio) were used as calibrators for SARS-CoV-2 antibody concentrations.
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bioRxiv - Genetics 2020Quote: ... 2015) was cultured in domes of Cultrex Pathclear Reduced Growth Factor Basement Membrane Extract (BME) (3533-001, Amsbio). Domes were covered by medium containing Advanced DMEM/F12 (Gibco) ...
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bioRxiv - Genetics 2020Quote: ... Each PGC line was in culture for approximately 3 weeks before freezing in aliquots of 50,000 cells resuspended in 125 μl of Stem-Cellbanker (Amsbio) and stored at −150 °C.
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bioRxiv - Cancer Biology 2020Quote: ... organoid cells were resuspended in basement membrane matrix BME (Amsbio) and plated as 15 μl droplets ...
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bioRxiv - Cell Biology 2020Quote: ... cell walls were digested for 30 minutes with 250 μg/ml zymolyase 100T (Amsbio; 120493-1) in zymolyase buffer (1.2M Sorbitol ...
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bioRxiv - Cell Biology 2020Quote: ... followed by spheroplasting for 90 min at 30°C in zymolyase buffer containing 0.43 mg/mL zymolyase 20T (Amsbio 120491-1). The resulting spheroplasts were washed once with 30°C zymolyase buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... 100-T Zymolyase (AMSBIO) was added to a final concentration of 1 mg/mL and incubated at 30°C for an additional 10 min ...