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Citations for Cambridge Isotope Labs :
251 - 300 of 341 citations for Trans Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%;1 D 97% 97% Chemical Purity since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Dried pellet from unlabeled samples was mixed with [U13C]-D/L-2HG (internal standard for unlabeled samples, Cambridge isotope laboratories ...
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bioRxiv - Biochemistry 2020Quote: ... or ‘heavy’ 13C615N2-Lysine (Silantes; 211604102) and 13C615N4-Arginine (Cambridge Isotope Labs Inc; CNLM-539-H-1). Cells were cultured in SILAC ‘light’ or ‘heavy’ media for two weeks prior to harvesting by centrifugation (800g ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell were depleted of methionine for 1h and then 1 mM L-azidohomoalanine (AHA, Cambridge Isotope Laboratories) was added to the media with a dose of CDCexo (103 EV/ cell ...
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bioRxiv - Microbiology 2024Quote: ... they were diluted 1:5 with an internal standard mixture (L-Adenine-8-13C (Cambridge Isotope Lab), L-Phenyl-d5-Alanine-2,3,3-d3 (Cambridge Isotope Lab) ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 15NH4Cl and when needed 13C-D-glucose as sole nitrogen and carbon sources (purchased from Cambridge Isotope Laboratories). The cells were grown at 37°C before they were induced with 0.2 mM IPTG at an OD600 of 1.2 for TB and 0.8 for M9 minimal medium and incubated at 17°C for 16 h.
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bioRxiv - Molecular Biology 2024Quote: EGM-2 media was replaced with DMEM media containing D-Glucose (U-13C6, CLM-1396-PK, Cambridge Isotope Laboratories Inc.) 3 hours prior to collection ...
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bioRxiv - Neuroscience 2023Quote: ... the powder was first dissolved in DMSO (100 mM, Cambridge Isotope Labs), which does not have a known effect on sensory axons or reflexes but was nonetheless kept at a minimum (final concentration <0.5%) ...
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bioRxiv - Microbiology 2019Quote: ... or 1 mM unlabeled methanol and 10 mM13C-labeled sodium bicarbonate (13C-labeled substrates provided by Cambridge Isotope Laboratories ...
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bioRxiv - Developmental Biology 2022Quote: ... flies were fed for 12hrs a mixture of 0.5M 13C-glucose (Cambridge Isotope Laboratories, Inc #CLM-1396-1) and 0.5M unlabeled glucose (Sigma #G5400) ...
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bioRxiv - Systems Biology 2023Quote: ... T87 cells were cultured in 15N-enriched NT-1 liquid medium containing K15NO3 and 15NH415NO3 (Cambridge Isotope Labs), under identical culture conditions ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated with media containing the designated tracer γ-15N-glutamine (Cambridge Isotope Laboratories: NLM-557-1), or U-13C-glucose (Cambridge Isotope Laboratories ...
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bioRxiv - Biochemistry 2022Quote: ... Cell pellets were resuspended in 250 mL of isotopically labelled minimal media M9 salt solution supplemented with 13C D-glucose 4.0 g/L and 15NH4Cl 1.0 g/L (Cambridge Isotope Laboratories, Inc.), then incubated to allow the recovery of growth and clearance of unlabeled metabolites ...
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bioRxiv - Molecular Biology 2020Quote: ... cultures were first grown at 37°C in 1 L of LB media until an OD600 of 0.6 before the cells were pelleted and resuspended in 0.5 L M9 minimal media prepared in D2O supplemented with 13C-D-glucose and 15NH4Cl (Cambridge Isotope Laboratories). After 1 hour recovery at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... 450 μL of supernatant were transferred to a new tube and 150 μL of deuterated water were added (D2O, D, 99.9%, Cambridge Isotope Laboratories). Each sample was vortex-mixed for 1 min before transferring into 5 mm SampleJet NMR tubes ...
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bioRxiv - Neuroscience 2023Quote: ... Experiments with deuterated lipids were conducted by replacing glucose with 10mM D-glucose (U-13C6, Cambridge isotope laboratories CLM-1396-PK) for 24h hours prior to treatment with 5 μM Aβ fibrils or no treatment.
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bioRxiv - Cancer Biology 2023Quote: ... no-glucose and no-glutamine media was supplemented with 5 mM of 13C6 D-Glucose (Cambridge Isotope Laboratories, Inc., CLM-1396) and 4 mM unlabeled glutamine ...
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bioRxiv - Cancer Biology 2023Quote: U-[13C]-Glc labelling media consisted of DMEM supplemented with 10 mM D-glucose (U-13C6; CLM-1396; Cambridge Isotope Laboratories), 2 mM glutamine ...
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bioRxiv - Cell Biology 2020Quote: ... 100 μl volume uptake reactions with 50 μM 13C5-NAD+ (Cambridge Isotope Laboratories) were initiated by adding substrate to 80 μg of mitochondria in incubation media containing 0.1% BSA and 100 μM unlabeled NAD+ at 30°C ...
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bioRxiv - Biophysics 2021Quote: ... Deuterated RNA samples were prepared in the same way using selective deuterated rNTPs (D-H5, H3′, H4′, H5′ and H5′′) and 13C/15N-labeled samples were prepared using labeled rNTPs (Cambridge Isotope Laboratories). The resulting RNAs were purified for NMR studies by gel electrophoresis ...
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bioRxiv - Biophysics 2019Quote: ... and uniformly 15N- or 15N,13C-labeled proteins were purified from cells grown in M9 minimal media with 15N ammonium chloride and/or 13C D-glucose (Cambridge Isotope Labs) as the sole nitrogen and/or carbon source ...
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bioRxiv - Microbiology 2021Quote: ... and Rpk9-I53-50A trimers were H/D exchanged (HDX) in the deuteration buffer (pH* 7.5, 85% D2O, Cambridge Isotope Laboratories, Inc.) for 3 ...
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bioRxiv - Immunology 2020Quote: 3 µg of monomeric RBD and RBD-8GS-I53-50A were incubated and H/D exchanged (HDX) in the deuteration buffer (pH* 7.6, 85% D2O, Cambridge Isotope Laboratories, Inc.) for 3 ...
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bioRxiv - Microbiology 2021Quote: ... Commercial reagents and standards for analysis were purchased from the following suppliers: Deuterated Dimethylsulfoxide (DMSO-d6, 99.9%) and 4,4-dimethyl-4-silapentane-1-sulfonic acid (Cambridge Isotope Laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21(DE3)pLysS cells in standard M9 minimal medium using 1 g/l 15N NH4Cl (Cambridge Isotope Laboratories) as a sole nitrogen source ...
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bioRxiv - Immunology 2024Quote: ... and replated in media containing the heavy isotope of interest (13C6-glucose (Cambridge Isotope Laboratories, Inc., CLM-1396-1) and 13C5-glutamine (CK Isotopes ...
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bioRxiv - Bioengineering 2021Quote: ... 5 μL of 100/3 mM DSS-D6 in deuterium oxide (Cambridge Isotope Laboratories) were added to 1.7 mm NMR tubes (Bruker BioSpin) ...
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bioRxiv - Molecular Biology 2020Quote: ... Unlabeled samples were prepared using Luria Broth (LB) (Melford) and minimal media M9 with 15NH4Cl and/or D- [13C] glucose (Cambridge Isotope Laboratories, Inc) were used to prepare the labeled samples (25) ...
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bioRxiv - Microbiology 2020Quote: ... the experiment was additionally repeated in media made from 18O enriched water (OLM-240-10-1, Cambridge Isotope Laboratories, Inc.) at a final δ18Owater of approximately +100‰.
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bioRxiv - Systems Biology 2019Quote: ... 20nM isotope-labeled fatty acid standards (U-13C-C16:0, U-13C-C18:1, U-13C-C18:2; Cambridge Isotope) were added before saponification as internal standards ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mM MgCl2, 0.5 mM EDTA, 0.5 mM ATP, 1 mM DTT, 10% glycerol [v/v], D2O [Cambridge Isotope Laboratories]) at 25°C and quenched by a 2-fold dilution with ice-cold quench buffer (0.8 M guanidine hydrochloride ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were labeled with 40 mM isotopically distinct dimethyl formaldehyde (light, CH2O, for the parental RPE-1 cells (Cambridge Isotope Laboratories ...
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bioRxiv - Plant Biology 2023Quote: ... The following isotope-labelled standards were added at 1 pmol per sample: 13C6-IAA (Cambridge Isotope Laboratories, Tewksbury, MA, USA); 2H4-SA (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... and resuspended in different volumes of water containing labeled non-standard amino acid mix (Cambridge Isotope Laboratories, MSK-NCAA-1) to account for differences in starting tissue weight.
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bioRxiv - Biophysics 2024Quote: ... 1 L cultures of autoinduction media (Cold Spring Harbor Protocols) were used with 15N labeled ammonium chloride (Cambridge Isotope Laboratories). The final antibiotic concentration was increased to 0.1 mg/mL of kanamycin to reduce plasmid loss and poor yields ...
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bioRxiv - Microbiology 2023Quote: ... 2.8 ml of gas was removed and immediately replaced with 2.8 ml of 15N2 (98% enriched, Cambridge Isotope Laboratories Inc, USA). Vials were overpressured by adding 0.3 ml of air or N2 gas in oxic or anoxic incubations ...
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bioRxiv - Biophysics 2021Quote: ... 100 µL of 140 mM [6,6’-2H2]glucose solution (Cambridge Isotope Laboratories, Tewksbury, MA, USA) was injected into each tube for a final 2H-labeled glucose concentration of 20 mM (and final volume=700 µL ...
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bioRxiv - Biophysics 2021Quote: ... the RNA samples were lyophilized and dissolved in 100% D2O (99.8%, Cambridge Isotope Laboratories, Inc.) and transferred to an Eppendorf tube containing lyophilized buffer (50 mM K-phosphate buffer (pH=7.5 or pH=5.8 ...
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bioRxiv - Biochemistry 2020Quote: ... Spectra were recorded either under conditions of neutral pH in 100% D2O (Cambridge Isotope Laboratories), or following alkalinization (i.e ...
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bioRxiv - Biophysics 2023Quote: ... The exo-PS accumulated on the 10 kDa membrane surface as a clear gel and was collected and dialyzed extensively against MilliQ-grade H2O or D2O (Cambridge Isotope Labs, 99.9% D). The H2O solutions provide scattering profiles at an additional contrast ...
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bioRxiv - Cell Biology 2023Quote: For whole cell proteomics lysine auxotrophic strains were grown in yeast nitrogen base (YNB) medium containing either 30 mg/ml L-Lysine or 30 mg/ ml 13C6;15N2-L-Lysine (Cambridge Isotope Laboratories, USA). Cells were grown to logarithmic phase and 1 OD unit of each strain was harvested ...
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bioRxiv - Cell Biology 2023Quote: Lysine auxotrophic strains were grown in yeast nitrogen base (YNB) medium containing either 30 mg/ml L-Lysine or 30 mg/ml 13C6;15N2-L-Lysine (Cambridge Isotope Laboratories, USA). Cells were grown to logarithmic phase and up to 350 OD600 units were harvested of labeled cells ...
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bioRxiv - Cell Biology 2023Quote: ... lysine auxotrophic strains were grown in yeast nitrogen base (YNB) medium containing either 30 mg/ml L-Lysine or 30 mg/ml 13C6;15N2-L-Lysine (Cambridge Isotope Laboratories, USA). Cells were grown to logarithmic phase and the same amount of OD units of the control strain and the mutant strain were harvested and combined ...
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bioRxiv - Microbiology 2021Quote: ... each of which was 25% labeled with 13C (i.e. mixed 1:4 with 25% H13CO3, 13C12H6O12, or CH313CO2, respectively; Cambridge Isotope Laboratories). All saltwater media was adapted from the Halohandbook and contained NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... Extraction solution contained 80:20 methanol:water with 1:2500 dilution of a heavy amino acid mix standard (MSK-A2, Cambridge Isotope Laboratories); derivatization solution is described below ...
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bioRxiv - Cancer Biology 2022Quote: ... Omental tumor biopsies from HGSOC patients were sectioned (0.5-1 mm) and incubated in DMEM supplemented with 10 mM [U-13C]-glucose (Cambridge Isotope Laboratories) and 2 mM glutamine ...
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bioRxiv - Cancer Biology 2023Quote: ... Two transitions were optimised using an authentic standard of glycocholic acid (glycine-1-13C, CLM-191-PK, Cambridge Isotope Laobratories, UK), from the negative precursor ion (m/z 465 ...
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bioRxiv - Biochemistry 2021Quote: ... with inclusion of 3 µL 100 µM labeled (13C,15N) amino acid standard mix (Cambridge Isotope Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were washed with 0.9% NaCl twice and lysed with pre-chilled extraction buffer that was composed of 480 ul of MeOH mixed with 120 ul of H2O and 1 uM of pre-mixed Heavy Amino Acid mix (Cambridge Isotope Laboratories). Lysates were transferred to an Eppendorf tube and vortexed at 4 °C for 10 min before being centrifuged at 16,000 g at 4 °C for 10 min ...
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bioRxiv - Developmental Biology 2021Quote: ... /water (2:2:1) containing isotope-labeled internal standards ([U-13C6]glucose; [U-13C3]lactate; [U-13C5]glutamine; and [U-13C5]glutamate; Cambridge Isotope Laboratories) were spiked into media samples ...
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bioRxiv - Biochemistry 2022Quote: ... modified glucose-free DMEM (Hyclone) was adjusted to 25 mM glucose with either 1-13C-glucose or 6-13C-glucose (Cambridge Isotope Laboratories). Cells were reverse transfected as described above ...